Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,375,947 A→G intergenic (+18/‑38) PA14_RS06470 → / → PA14_RS06475 PspA/IM30 family protein/DUF1449 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,375,9470AG99.8% 1653.1 / NA 420intergenic (+18/‑38)PA14_RS06470/PA14_RS06475PspA/IM30 family protein/DUF1449 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (193/226);  minor base T (1/0);  total (194/226)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 427 total aligned reads displayed.

GGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGA  >  NC_008463/1375800‑1376093
                                                                                                                                                   |                                                                                                                                                  
ggCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCgaa                                                                                                                                                  >  2:2793317/1‑150 (MQ=255)
ggCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCgaa                                                                                                                                                  >  2:4626752/1‑150 (MQ=255)
ggCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCgaa                                                                                                                                                  <  1:9412115/150‑1 (MQ=255)
 gCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAg                                                                                                                                                 >  2:3521218/1‑150 (MQ=255)
  ccGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAgg                                                                                                                                                <  1:2621092/150‑1 (MQ=255)
     aaCTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGt                                                                                                                                             >  1:11113548/1‑150 (MQ=255)
      aCTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCggc                                                                                                                                            >  1:1468452/1‑148 (MQ=255)
       cTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTcc                                                                                                                                           <  1:7773757/150‑1 (MQ=255)
       cTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTcc                                                                                                                                           <  2:10780983/150‑1 (MQ=255)
       cTGGCCAGCGCGGCCGCGCCGGCCGAGGCGCTGGACGCCAAGCGGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCGGGCGCGCCTGAAGCAAAAGGGCAAGGCCGGATAGGCCCGCGGCCCGGCGAAGGCGTcc                                                                                                                                           <  2:6908652/150‑1 (MQ=255)
           ccAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGc                                                                                                                                       <  2:9505562/150‑1 (MQ=255)
             aGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGccc                                                                                                                                     >  2:3307198/1‑150 (MQ=255)
             aGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGccc                                                                                                                                     >  2:1791052/1‑150 (MQ=255)
             aGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGccc                                                                                                                                     <  1:4833739/150‑1 (MQ=255)
               cgcgGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGt                                                                                                                                   <  2:2416428/150‑1 (MQ=255)
                gcggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGtt                                                                                                                                  >  1:3233123/1‑150 (MQ=255)
                 cggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGttt                                                                                                                                 >  2:10531671/1‑150 (MQ=255)
                   gcggcgCCGCACGATGCGCTGGCCGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAACAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGttttt                                                                                                                               <  2:11191922/150‑1 (MQ=255)
                      gcgcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAg                                                                                                                            <  2:7383288/150‑1 (MQ=255)
                       cgcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAgg                                                                                                                           >  2:1319050/1‑150 (MQ=255)
                        gcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAggg                                                                                                                          >  2:7238030/1‑150 (MQ=255)
                        gcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAggg                                                                                                                          <  2:4290727/150‑1 (MQ=255)
                            gACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATag                                                                                                                      >  1:9983938/1‑150 (MQ=255)
                              cGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATagag                                                                                                                    >  1:2799802/1‑150 (MQ=255)
                                 tGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGa                                                                                                                 <  1:5566748/150‑1 (MQ=255)
                                   cgcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACa                                                                                                               <  1:2100280/150‑1 (MQ=255)
                                   cgcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACa                                                                                                               <  2:1791881/150‑1 (MQ=255)
                                    gcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACaa                                                                                                              <  2:5821969/150‑1 (MQ=255)
                                    gcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACaa                                                                                                              <  2:5073342/150‑1 (MQ=255)
                                     cTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAAt                                                                                                             >  1:4369344/1‑150 (MQ=255)
                                       ggACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATgg                                                                                                           <  1:3969609/150‑1 (MQ=255)
                                           gCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAgg                                                                                                       <  1:7624239/150‑1 (MQ=255)
                                           gCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAgg                                                                                                       >  1:10005927/1‑150 (MQ=255)
                                             cacgCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCCAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGATTTTCAGGGATAGAGCGACAATGGAAGGtt                                                                                                     <  2:708602/147‑1 (MQ=255)
                                             cAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGtt                                                                                                     >  1:2272136/1‑150 (MQ=255)
                                              aaGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAATGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGttt                                                                                                    >  2:1243278/1‑150 (MQ=255)
                                                gCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTcc                                                                                                  <  1:7161959/150‑1 (MQ=255)
                                                  tGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTg                                                                                                >  2:5977801/1‑150 (MQ=255)
                                                   gcgcgcGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGc                                                                                               >  1:4061367/1‑150 (MQ=255)
                                                    cgcgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCa                                                                                              <  1:2020075/150‑1 (MQ=255)
                                                    cgcgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCGGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCa                                                                                              <  1:91175/150‑1 (MQ=255)
                                                     gcgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGGCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAg                                                                                             >  1:1304730/1‑150 (MQ=255)
                                                        cgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACg                                                                                          <  2:2310868/150‑1 (MQ=255)
                                                            cGGGATCAAGGCCTCGGGCCACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGgcgc                                                                                      <  2:6530970/150‑1 (MQ=255)
                                                              ggATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTg                                                                                    <  2:11093337/150‑1 (MQ=255)
                                                                 tCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCg                                                                                 <  1:7104142/150‑1 (MQ=255)
                                                                  cAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGt                                                                                >  2:8087719/1‑150 (MQ=255)
                                                                    aGGACTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCATGGACCGTTTTTCTGGGATAGTGCGCCATTGGATGTTTTCCTGCAGACGGCGCTGTCGttt                                                                              <  2:9683627/150‑1 (MQ=255)
                                                                       ccTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCg                                                                           <  1:10139306/150‑1 (MQ=255)
                                                                             gCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTg                                                                     <  1:10952513/150‑1 (MQ=255)
                                                                              cAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGc                                                                    <  2:9136255/150‑1 (MQ=255)
                                                                              cAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGc                                                                    <  1:1223305/150‑1 (MQ=255)
                                                                               aaCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCt                                                                   >  2:2055994/1‑150 (MQ=255)
                                                                                aCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGCAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTg                                                                  >  2:4957424/1‑150 (MQ=255)
                                                                                aCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTg                                                                  >  1:3571219/1‑150 (MQ=255)
                                                                                aCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTg                                                                  <  2:5475111/150‑1 (MQ=255)
                                                                                   gCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTc                                                               <  1:6826490/150‑1 (MQ=255)
                                                                                    cGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCa                                                              <  1:1680731/150‑1 (MQ=255)
                                                                                      tGGATGGCGTGCTGGCGCGCCTGAAGGAAATGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGc                                                            >  2:9404877/1‑150 (MQ=255)
                                                                                      tGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGc                                                            >  1:10403558/1‑150 (MQ=255)
                                                                                      tGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGc                                                            <  2:4888250/150‑1 (MQ=255)
                                                                                        gATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCtt                                                          <  1:6685772/150‑1 (MQ=255)
                                                                                         aTGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGTCCGTGCTGTTCTGCtgt                                                         >  2:10996359/1‑148 (MQ=255)
                                                                                         aTGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCttt                                                         <  1:4934581/150‑1 (MQ=255)
                                                                                           ggCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTttct                                                       <  1:8815129/150‑1 (MQ=255)
                                                                                              gTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTtcttct                                                    >  2:2564600/1‑150 (MQ=255)
                                                                                               tGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTg                                                   <  2:3247060/150‑1 (MQ=255)
                                                                                                 caggCGCGCCTGAAGGAAAAGGGCAAGGACTGATAGGCCCGCTGCCCGGCTAAGGCGTCAGGGCCCGTTTTTAAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGATCAGCTTTCTTCTGAt                                                 >  2:4108650/3‑150 (MQ=255)
                                                                                                  tGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTATCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATc                                                >  1:5237191/1‑150 (MQ=255)
                                                                                                      gcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGACGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcg                                            <  1:6112641/150‑1 (MQ=255)
                                                                                                      gcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcg                                            >  2:9318644/1‑150 (MQ=255)
                                                                                                        gcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcgcg                                          >  1:8437489/1‑150 (MQ=255)
                                                                                                         ccTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGa                                         >  2:9857680/1‑150 (MQ=255)
                                                                                                         ccTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGa                                         <  2:6160202/150‑1 (MQ=255)
                                                                                                         ccTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCGTTCTTCTGATCCTCGCGa                                         >  1:1081171/1‑150 (MQ=255)
                                                                                                              aGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatcatc                                    >  1:8572394/1‑150 (MQ=255)
                                                                                                                 aaaaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTAc                                 <  1:2430530/150‑1 (MQ=255)
                                                                                                                 aaaaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTAc                                 >  2:8606197/1‑150 (MQ=255)
                                                                                                                   aaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTg                               >  1:8316818/1‑150 (MQ=255)
                                                                                                                    aGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgg                              <  1:1061125/150‑1 (MQ=255)
                                                                                                                    aGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgg                              <  1:5919992/150‑1 (MQ=255)
                                                                                                                    aGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgg                              >  2:7869938/1‑150 (MQ=255)
                                                                                                                     gggCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTggg                             <  1:3104337/150‑1 (MQ=255)
                                                                                                                      ggCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgggg                            <  1:9255393/150‑1 (MQ=255)
                                                                                                                      ggCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgggg                            <  1:6373655/150‑1 (MQ=255)
                                                                                                                         aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAt                         >  1:3701593/1‑150 (MQ=255)
                                                                                                                         aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAt                         >  2:6709185/1‑150 (MQ=255)
                                                                                                                         aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAt                         <  2:791573/150‑1 (MQ=255)
                                                                                                                          aGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtc                        >  1:5855717/1‑150 (MQ=255)
                                                                                                                                gATAGGCCCGCGGCCCGGCGACGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCGGTTCAGCGTGCGTCTGATCCTCGCGATCCTCTACTGGGGCATCGTCGcc                  >  2:10405377/1‑150 (MQ=255)
                                                                                                                                gATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGcc                  >  1:8501882/1‑150 (MQ=255)
                                                                                                                                gATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGcc                  >  2:162065/1‑150 (MQ=255)
                                                                                                                                  tAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCt                >  2:2449065/1‑150 (MQ=255)
                                                                                                                                     gCCCGCGGCCCGGCGAAGTCGTCGGGGCCGGTTTTTCAGGGATAGAGCGTCAATGGAAGGTTTCCTGCTGTCGGCGCTGTAGTTTCCGACTGTGATGTTCAGCTTTCTTCTGATCCTCGCGATCATATACTGGGGCAACGTCGCCCTggg             <  1:4431853/150‑1 (MQ=255)
                                                                                                                                     gCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTggg             <  1:139711/150‑1 (MQ=255)
                                                                                                                                        cgcgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCAt          <  1:8021540/150‑1 (MQ=255)
                                                                                                                                         gcgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATg         <  2:7528410/150‑1 (MQ=255)
                                                                                                                                           ggCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGt       <  1:9884823/150‑1 (MQ=255)
                                                                                                                                                ggCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCgaga  <  1:9655187/150‑1 (MQ=255)
                                                                                                                                                ggCGAAGGCGTCCGGGCCCGTTTTTCAGGGAGAGAGCGACAATGGAAGGTTTCCTGCTGACGGCGCTGTCGTTTCCGACCGTGCAGTTCAGCTTTCTTCTGTTCCTCGCGATCAGCTCCTGGGGCATCGTCGCCCTGGGGATGGTCgtgt  >  2:8807860/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGA  >  NC_008463/1375800‑1376093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑