BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,001,811	A→C	Q66P (CAG→CCG)	pgl →	6‑phosphogluconolactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,001,811	0	A	C	100.0%	1583.3 / NA	406	Q66P (CAG→CCG)	pgl	6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (185/221); total (185/221)

Only 100 of 406 total aligned reads displayed.

CAGCGCCGCCGAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGATCAGGTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCCTCTACCAG  >  NC_008463/2001668‑2001953

 aGCGCCGCCTAGCAGGCCCGCTGCCGGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCTAGCAGGGCCAGGCGCTGCTGTTGGTGTCCGGTGGGCGCAGTCAGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGAc                                                                                                                                         <  2:4233715/150‑1 (MQ=255)

  gcgcCGCCGAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACt                                                                                                                                        >  1:9573247/1‑150 (MQ=255)

  gcgcCGCCGAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACt                                                                                                                                        >  1:9045102/1‑150 (MQ=255)

     ccgccgAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGt                                                                                                                                     >  2:3163221/1‑150 (MQ=255)

        ccgAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTcgc                                                                                                                                  >  1:2471361/1‑150 (MQ=255)

          gAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCgg                                                                                                                                >  2:2422328/1‑150 (MQ=255)

           aGCAGGCCCGCGTCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGa                                                                                                                               <  1:9794724/150‑1 (MQ=255)

           aGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGa                                                                                                                               <  2:2490680/150‑1 (MQ=255)

                gCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCAcc                                                                                                                          >  2:1467401/1‑150 (MQ=255)

                  ccGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCt                                                                                                                        <  1:3944198/150‑1 (MQ=255)

                   cgcgGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTc                                                                                                                       <  1:8888447/150‑1 (MQ=255)

                    gcgGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCa                                                                                                                      >  1:6015752/1‑150 (MQ=255)

                     cgGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAg                                                                                                                     <  1:8773843/150‑1 (MQ=255)

                       gCCGGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGcc                                                                                                                   <  2:5400545/150‑1 (MQ=255)

                        ccTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCt                                                                                                                  <  1:10433051/150‑1 (MQ=255)

                        ccTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCt                                                                                                                  <  2:2130569/150‑1 (MQ=255)

                        ccTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCt                                                                                                                  <  1:7090273/150‑1 (MQ=255)

                         cTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTg                                                                                                                 >  2:8961901/1‑150 (MQ=255)

                             ccgccgAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCcg                                                                                                             >  1:8337174/1‑150 (MQ=255)

                               gccgAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCcgac                                                                                                           <  2:7244563/150‑1 (MQ=255)

                               gccgAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCcgac                                                                                                           <  2:7713045/150‑1 (MQ=255)

                               gccgAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCcgac                                                                                                           >  2:8445360/1‑150 (MQ=255)

                                  gAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAg                                                                                                        <  2:1386766/150‑1 (MQ=255)

                                   aGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAgc                                                                                                       >  2:6867757/1‑150 (MQ=255)

                                    ggTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAgcg                                                                                                      <  2:1295048/150‑1 (MQ=255)

                                    ggTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAgcg                                                                                                      <  2:10409457/150‑1 (MQ=255)

                                         ccggccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTggg                                                                                                 <  2:3537292/150‑1 (MQ=255)

                                         ccggccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTggg                                                                                                 <  2:8890303/150‑1 (MQ=255)

                                         ccggccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTggg                                                                                                 <  1:7343225/150‑1 (MQ=255)

                                          cggccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGt                                                                                                <  1:3404652/150‑1 (MQ=255)

                                           ggccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTg                                                                                               <  2:4704405/150‑1 (MQ=255)

                                           ggccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTg                                                                                               >  1:10315010/1‑150 (MQ=255)

                                           ggccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTg                                                                                               <  1:5319225/150‑1 (MQ=255)

                                           ggccggTTGCGCTCGGCGCTGGCAGAGCTGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGAATGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTg                                                                                               <  2:5918299/150‑1 (MQ=255)

                                             ccggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGcc                                                                                             >  2:2973023/1‑150 (MQ=255)

                                               ggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCgg                                                                                           <  1:10802955/150‑1 (MQ=255)

                                               ggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCgg                                                                                           <  1:4964116/150‑1 (MQ=255)

                                               ggTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCgg                                                                                           >  2:6128752/1‑150 (MQ=255)

                                                gTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGa                                                                                          <  1:3194825/150‑1 (MQ=255)

                                                gTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGa                                                                                          >  1:10009282/1‑150 (MQ=255)

                                                gTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGa                                                                                          <  2:6669709/150‑1 (MQ=255)

                                                   gcgcTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTc                                                                                       >  2:7993364/1‑150 (MQ=255)

                                                   gcgcTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTc                                                                                       <  2:2844979/150‑1 (MQ=255)

                                                      cTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCa                                                                                    <  1:8577336/150‑1 (MQ=255)

                                                        cGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATg                                                                                  >  1:1069230/1‑150 (MQ=255)

                                                          gcgcTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGcc                                                                                <  2:2806193/150‑1 (MQ=255)

                                                            gcTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGa                                                                              >  2:5793783/1‑150 (MQ=255)

                                                            gcTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGa                                                                              <  1:9893078/150‑1 (MQ=255)

                                                             cTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGAt                                                                             >  2:4412525/1‑150 (MQ=255)

                                                              tGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATa                                                                            <  2:3936292/150‑1 (MQ=255)

                                                               ggCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAg                                                                           >  2:1992372/1‑150 (MQ=255)

                                                                gCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGc                                                                          <  2:10693240/150‑1 (MQ=255)

                                                                gCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGc                                                                          <  2:5016615/150‑1 (MQ=255)

                                                                gCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGc                                                                          <  1:9295835/150‑1 (MQ=255)

                                                                   gAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAATCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAAc                                                                       >  1:571473/1‑150 (MQ=255)

                                                                    cgCAGGGCCAGGCGCTGCTGGTGGGGTCCGGTGGGCGCCGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGCCTGGTCGCGTATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCACCg                                                                      <  2:11188092/149‑1 (MQ=255)

                                                                    aGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACg                                                                      <  2:9197298/150‑1 (MQ=255)

                                                                      cAGGGCCCGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTGGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCATCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGcc                                                                    <  2:4838544/150‑1 (MQ=255)

                                                                      cAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGAGAGCAACGcc                                                                    <  1:4122269/150‑1 (MQ=255)

                                                                       aGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCg                                                                   >  1:7468007/1‑150 (MQ=255)

                                                                        gggCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGCAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCgg                                                                  <  1:4537244/150‑1 (MQ=255)

                                                                         ggCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGc                                                                 >  1:13402/1‑150 (MQ=255)

                                                                             aGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTgg                                                             >  2:10543361/1‑150 (MQ=255)

                                                                              ggCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGt                                                            >  1:760413/1‑150 (MQ=255)

                                                                                cgcTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTc                                                          <  1:1568270/150‑1 (MQ=255)

                                                                                cgcTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTCGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTc                                                          <  1:5876751/150‑1 (MQ=255)

                                                                                    gctgGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTcgccg                                                      >  1:6633368/1‑150 (MQ=255)

                                                                                          ggtgTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCTACGCCGGCCTGGTTCGCCGCCACCt                                                <  1:6845860/150‑1 (MQ=255)

                                                                                            ggtCCGGTGGGCGCAGTCCGGTGGCCGTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGc                                              <  2:8195261/149‑1 (MQ=255)

                                                                                             gtCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCt                                             <  2:2657652/150‑1 (MQ=255)

                                                                                              tCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGTCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTc                                            >  1:736606/1‑150 (MQ=255)

                                                                                               ccGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTcc                                           >  1:8052464/1‑150 (MQ=255)

                                                                                                 ggTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGt                                         <  2:4680225/150‑1 (MQ=255)

                                                                                                  gTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTg                                        >  1:9180702/1‑150 (MQ=255)

                                                                                                   tGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTgg                                       <  1:2717102/150‑1 (MQ=255)

                                                                                                      gcgcAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGa                                    <  2:9622013/150‑1 (MQ=255)

                                                                                                         cAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAggc                                 >  1:9399985/1‑150 (MQ=255)

                                                                                                          aGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAggcg                                <  2:7822115/150‑1 (MQ=255)

                                                                                                           gTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAggcgg                               <  1:2975324/150‑1 (MQ=255)

                                                                                                           gTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAggcgg                               >  1:4313729/1‑150 (MQ=255)

                                                                                                           gTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAggcgg                               <  1:5546968/150‑1 (MQ=255)

                                                                                                                  ggCCTTCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGgc                        <  2:8034662/150‑1 (MQ=255)

                                                                                                                      ttCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGgcgcgc                    >  2:10144754/1‑150 (MQ=255)

                                                                                                                      ttCCTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGgcgcgc                    <  2:11073412/150‑1 (MQ=255)

                                                                                                                         cTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTc                 <  2:10072949/150‑1 (MQ=255)

                                                                                                                         cTCGAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTc                 <  2:10827489/150‑1 (MQ=255)

                                                                                                                            gAAGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATc              >  1:1645823/1‑150 (MQ=255)

                                                                                                                             aaGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCg             <  2:6505065/150‑1 (MQ=255)

                                                                                                                             aaGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCg             >  2:94845/1‑150 (MQ=255)

                                                                                                                             aaGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCg             <  2:1726615/150‑1 (MQ=255)

                                                                                                                              aGCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCgg            <  1:1956893/150‑1 (MQ=255)

                                                                                                                               gCCTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGCGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGc           <  1:1255792/150‑1 (MQ=255)

                                                                                                                                 cTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCct         <  1:6497191/150‑1 (MQ=255)

                                                                                                                                 cTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCct         >  2:10239162/1‑150 (MQ=255)

                                                                                                                                 cTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCct         >  2:3301943/1‑150 (MQ=255)

                                                                                                                                 cTTGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCct         >  1:4036150/1‑150 (MQ=255)

                                                                                                                                   tGAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCctct       >  2:930495/1‑150 (MQ=255)

                                                                                                                                    gAGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCCTCTa      >  1:1787032/1‑150 (MQ=255)

                                                                                                                                     aGCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTAAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGCAGGCGCGCTTCATCGGCCTCTAc     >  2:5139928/1‑150 (MQ=255)

                                                                                                                                      gCGAGGAGCCGCTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCCTCTAcc    >  2:1868189/1‑150 (MQ=255)

CAGCGCCGCCGAGCAGGCCCGCGGCCTGGCCGCCGAGGTCGCCGGCCGGTTGCGCTCGGCGCTGGCCGAGCAGGGCCAGGCGCTGCTGGTGGTGTCCGGTGGGCGCAGTCCGGTGGCCTTCCTCGAAGCCTTGAGCGAGGATCAGGTGGACTGGTCGCGGATCACCCTCAGCCTGGCCGACGAGCGCTGGGTGCCGGAGTCGCATGCCGATAGCAACGCCGGCCTGGTTCGCCGCCACCTGCTCCGTGGCGAGGCGGCGAAGGCGCGCTTCATCGGCCTCTACCAG  >  NC_008463/2001668‑2001953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`