BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	96,360	T→C	pseudogene (274/723 nt)	tagH ←	type VI secretion system‑associated FHA domain protein TagH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	96,360	0	T	C	99.8%	2322.2 / NA	589	pseudogene (274/723 nt)	tagH	type VI secretion system‑associated FHA domain protein TagH
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (259/329); minor base A (1/0); total (260/329)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 622 total aligned reads displayed.

GATCCGGCGCCATGAACGCCTGGTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACAGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGTTGTCCAGCGTGCGCAGCACGTCCACCAGTCCTTCCACCAGACAGCGATAGGTGCGTCGGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTC‑ACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAGGTCGCCGCCGGCCG  >  NC_008463/96213‑96505

  tCCGGCGCCATGAACGCCTGGTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCgcgcgcgc                                                                                                                                                >  2:6526149/1‑150 (MQ=255)

       cgcCATGAACGCCTGGTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCAc                                                                                                                                           <  1:9367076/150‑1 (MQ=255)

          cATGAACGCCTGGTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTc                                                                                                                                        >  2:2745869/1‑150 (MQ=255)

             gAACGCCTGGTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTccac                                                                                                                                     >  1:8167000/1‑150 (MQ=255)

                 gCCTGGTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGt                                                                                                                                 <  2:1774586/150‑1 (MQ=255)

                  ccTGGTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTc                                                                                                                                >  2:10808384/1‑150 (MQ=255)

                   cTGGTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTcc                                                                                                                               >  2:1941952/1‑150 (MQ=255)

                      gTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTc                                                                                                                            >  2:6906267/1‑150 (MQ=255)

                       ttGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTcc                                                                                                                           <  1:5124688/150‑1 (MQ=255)

                        tGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTcca                                                                                                                          <  1:10316600/150‑1 (MQ=255)

                        tGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTcca                                                                                                                          <  2:10418791/150‑1 (MQ=255)

                         gTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTccac                                                                                                                         <  2:4971875/150‑1 (MQ=255)

                          tCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTccacc                                                                                                                        <  1:2645499/150‑1 (MQ=255)

                          tCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCGTccacc                                                                                                                        <  2:8854250/150‑1 (MQ=255)

                               gcgcAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAggcc                                                                                                                   <  2:7561186/150‑4 (MQ=255)

                               gcgcAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAggcc                                                                                                                   >  1:10878943/1‑147 (MQ=255)

                                cgcAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAggccg                                                                                                                  >  1:6873054/1‑146 (MQ=255)

                                cgcAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAggccg                                                                                                                  <  1:1470905/150‑5 (MQ=255)

                                 gcagcaGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAggccgc                                                                                                                 <  2:4233764/150‑6 (MQ=255)

                                 gcagcaGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAggccgc                                                                                                                 <  2:6719551/150‑6 (MQ=255)

                                    gcagGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGAt                                                                                                              <  1:1710623/150‑1 (MQ=255)

                                     cagGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATa                                                                                                             >  2:7375474/1‑150 (MQ=255)

                                     cagGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATa                                                                                                             <  2:3821238/150‑1 (MQ=255)

                                        gAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAgct                                                                                                          >  1:7954933/1‑148 (MQ=255)

                                         agcagcATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTg                                                                                                         <  2:11054649/150‑1 (MQ=255)

                                         agcagcATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTg                                                                                                         >  2:4835014/1‑150 (MQ=255)

                                         agcagcATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTg                                                                                                         >  2:9029522/1‑150 (MQ=255)

                                          gcagcaTGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGc                                                                                                        >  2:6348745/1‑150 (MQ=255)

                                          gcagcaTGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGc                                                                                                        >  1:2205741/1‑150 (MQ=255)

                                          gcagcaTGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTCTTCAGCACCGGCTGGAGCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGc                                                                                                        >  2:5424067/1‑150 (MQ=255)

                                             gcaTGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCggc                                                                                                     <  1:11085441/150‑3 (MQ=255)

                                             gcaTGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCggc                                                                                                     <  1:9021340/150‑3 (MQ=255)

                                             gcaTGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCggc                                                                                                     <  1:8464245/150‑3 (MQ=255)

                                               aTGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCggccg                                                                                                   >  1:7855028/1‑146 (MQ=255)

                                               aTGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCggccg                                                                                                   >  2:1411813/1‑146 (MQ=255)

                                               aTGGCTTCGTCGACGGTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCggccg                                                                                                   <  1:609227/150‑5 (MQ=255)

                                                 ggCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGAt                                                                                                 <  2:8744524/150‑1 (MQ=255)

                                                  gCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATg                                                                                                >  2:1587578/1‑150 (MQ=255)

                                                       gtcgACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTc                                                                                           <  2:5225906/150‑1 (MQ=255)

                                                         cgACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCa                                                                                         <  1:6957239/150‑1 (MQ=255)

                                                         cgACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCa                                                                                         <  1:4446789/150‑1 (MQ=255)

                                                           aCGTTCGGCGCGACCTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATc                                                                                       <  1:7433583/150‑1 (MQ=255)

                                                              ttCGGCGCGAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTgc                                                                                    <  1:100397/150‑1 (MQ=255)

                                                                    gcgAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCg                                                                              <  1:4290696/150‑1 (MQ=255)

                                                                     cgAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCgg                                                                             >  1:771428/1‑150 (MQ=255)

                                                                      gAACTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGgc                                                                            >  1:9731013/1‑150 (MQ=255)

                                                                       aaCTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGgcg                                                                           >  2:4788934/1‑150 (MQ=255)

                                                                       aaCTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGgcg                                                                           >  2:2918319/1‑150 (MQ=255)

                                                                        aCTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGgcgc                                                                          <  1:2558793/150‑1 (MQ=255)

                                                                        aCTTCAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGgcgc                                                                          <  1:1957379/150‑1 (MQ=255)

                                                                            cAGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCgccggc                                                                      <  2:952317/150‑1 (MQ=255)

                                                                            cAGCGGGGTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCgccggc                                                                      <  1:2078316/150‑1 (MQ=255)

                                                                             aGCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCgccggcc                                                                     >  2:3396330/1‑150 (MQ=255)

                                                                              gCGGGTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCgccggccg                                                                    >  2:311152/1‑150 (MQ=255)

                                                                                gggTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCggg                                                                  <  1:2365691/150‑1 (MQ=255)

                                                                                gggTTGTTCTGCACCGGCTGGATCAGGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCggg                                                                  >  2:4524391/1‑150 (MQ=255)

                                                                                 ggTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCCGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGt                                                                 >  2:771375/1‑150 (MQ=255)

                                                                                 ggTTGTTCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGt                                                                 >  2:9663416/1‑150 (MQ=255)

                                                                                     gttCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCTGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGAc                                                             >  1:3731507/1‑150 (MQ=255)

                                                                                     gttCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGAc                                                             <  1:3304022/150‑1 (MQ=255)

                                                                                       tCTGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCt                                                           <  1:7068072/150‑1 (MQ=255)

                                                                                         tGCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTc                                                         >  1:7477224/1‑150 (MQ=255)

                                                                                          gCACCGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTC‑a                                                       >  2:207768/1‑150 (MQ=255)

                                                                                            accggCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTC‑aac                                                     >  1:2999462/4‑148 (MQ=255)

                                                                                              cggCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTC‑aactg                                                   <  1:5457790/149‑5 (MQ=255)

                                                                                              cggCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTC‑aactg                                                   <  1:9127337/149‑5 (MQ=255)

                                                                                                gCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTggg                                                  <  2:9391037/150‑1 (MQ=255)

                                                                                                gCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTggg                                                  <  1:4026841/150‑1 (MQ=255)

                                                                                                gCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTggg                                                  <  2:7164834/150‑1 (MQ=255)

                                                                                                   ggATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCa                                               <  1:10567581/150‑1 (MQ=255)

                                                                                                   ggATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCa                                               >  2:4108761/1‑150 (MQ=255)

                                                                                                     aTCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGCCCTTCAACTGGGTCATg                                             >  2:8138891/1‑150 (MQ=255)

                                                                                                     aTCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATg                                             <  1:4791704/150‑1 (MQ=255)

                                                                                                     aTCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATg                                             <  2:39255/150‑1 (MQ=255)

                                                                                                      tCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGc                                            >  1:2262166/1‑150 (MQ=255)

                                                                                                      tCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGc                                            >  1:5993606/1‑150 (MQ=255)

                                                                                                       cATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGcc                                           <  1:8822863/150‑1 (MQ=255)

                                                                                                           gTCTGCGCCATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGgcg                                       >  1:3128308/1‑150 (MQ=255)

                                                                                                                  ccATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCa                                <  2:1239977/150‑1 (MQ=255)

                                                                                                                  ccATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCa                                >  1:951170/1‑150 (MQ=255)

                                                                                                                   cATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAg                               <  1:8585918/150‑1 (MQ=255)

                                                                                                                   cATGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAg                               <  1:2870999/150‑1 (MQ=255)

                                                                                                                    aTGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAgg                              <  2:2572137/150‑1 (MQ=255)

                                                                                                                    aTGCGGAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAgg                              <  2:1118680/150‑1 (MQ=255)

                                                                                                                         gAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGc                         <  1:5859545/150‑1 (MQ=255)

                                                                                                                         gAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGc                         <  2:8211745/150‑1 (MQ=255)

                                                                                                                         gAACTCGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGc                         >  2:10120092/1‑150 (MQ=255)

                                                                                                                          caCTCGCCCTCCAGGCTCGCCCGCGCGCGCAGCACGACCACCACTCCTTCCACCAGGCCTCGATAGCTGCGGCCGAAGGCTTCCCTCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGcc                        <  2:5828683/149‑1 (MQ=255)

                                                                                                                              cGCCCTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTgca                    >  1:5564206/1‑150 (MQ=255)

                                                                                                                                 ccTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTgcagct                 >  1:6485343/1‑149 (MQ=255)

                                                                                                                                 ccTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTgcagca                 >  2:2277975/1‑150 (MQ=255)

                                                                                                                                  cTTCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGcagcag                >  1:7337233/1‑150 (MQ=255)

                                                                                                                                   ttCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAgg               >  2:3596143/1‑150 (MQ=255)

                                                                                                                                    tCAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAGGt              <  2:7814012/150‑1 (MQ=255)

                                                                                                                                     cAGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAGGTc             >  2:9166193/1‑150 (MQ=255)

                                                                                                                                      aGGCTGGCCCGCGCGCGCAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAGNNcg            >  2:4647436/1‑150 (MQ=255)

                                                                                                                                           ggcccgcgcgcgcAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAGGTCgccgcc       <  2:9787241/145‑1 (MQ=255)

                                                                                                                                            gcccgcgcgcgcAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAGGTCGccgccg      >  2:10673650/5‑150 (MQ=255)

                                                                                                                                             cccgcgcgcgcAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAGGTCGCCgccgg     <  2:8604082/147‑1 (MQ=255)

                                                                                                                                              ccgcgcgcgcAGCACGTCCACCAGTCCTTCCACCAGGCCGCGATAGCTGCGGCCGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTCAACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAGGTCGCCgccggc    <  1:7283401/148‑1 (MQ=255)

GATCCGGCGCCATGAACGCCTGGTTGTCGCGGCGCAGCAGGAGCAGCATGGCTTCGTCGACGTTCGGCGCGAACTTCAGCGGGTTGTTCTGCACAGGCTGGATCATGGTCTGCGCCATGCGGAACTCGCCCTTCAGGTTGTCCAGCGTGCGCAGCACGTCCACCAGTCCTTCCACCAGACAGCGATAGGTGCGTCGGATGGCTTCCATCTGCGCTTCGGCGCCGGCCGGGTCGACCTTC‑ACTGGGTCATGCCGGCGCCGCGCAGGAACGCCTGCAGCAGGTCGCCGCCGGCCG  >  NC_008463/96213‑96505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`