Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,150,987 Δ1 bp intergenic (‑192/+24) pvcA ← / ← PA14_RS14420 paerucumarin biosynthesis protein PvcA/asparaginase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,150,9850C.99.7% 1900.9 / ‑0.7 387intergenic (‑190/+26)pvcA/PA14_RS14420paerucumarin biosynthesis protein PvcA/asparaginase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base . (233/152);  minor base G (0/1);  total (233/154)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 395 total aligned reads displayed.

GGCAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGCCCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTCCACCA  >  NC_008463/3150835‑3151131
                                                                                                                                                      |                                                                                                                                                  
ggCAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGc                                                                                                                                                    <  1:3630428/151‑1 (MQ=255)
     cGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGccgccg                                                                                                                                               <  2:2267615/151‑5 (MQ=255)
      ggAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCcgg                                                                                                                                             <  1:2694894/151‑1 (MQ=255)
      ggAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCcgg                                                                                                                                             <  1:8086151/151‑1 (MQ=255)
            cGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCggcgggcg                                                                                                                                       <  1:8402724/151‑1 (MQ=255)
             gACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGgcgggcgg                                                                                                                                      >  1:31942/1‑151 (MQ=255)
             gACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGgcgggcgg                                                                                                                                      <  1:7198960/151‑1 (MQ=255)
               cATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGcc                                                                                                                                    <  1:7076198/151‑1 (MQ=255)
                  ccTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGACGGAAGCGG‑CCGCCGGCGGGCGGCCGCa                                                                                                                                 >  1:9231878/1‑151 (MQ=255)
                  ccTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCa                                                                                                                                 >  1:1825127/1‑151 (MQ=255)
                        gACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCgg                                                                                                                           >  2:6481830/1‑151 (MQ=255)
                          cgcgccgcCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTGGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTc                                                                                                                         <  2:3888689/149‑1 (MQ=255)
                           tCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCa                                                                                                                        >  1:2855050/1‑151 (MQ=255)
                             gccgccGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCAGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTAACGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGt                                                                                                                      >  2:723409/1‑151 (MQ=255)
                              ccgccgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTc                                                                                                                     <  2:9253001/151‑1 (MQ=255)
                               cgccgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCg                                                                                                                    <  1:9954602/151‑1 (MQ=255)
                                gccgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGGTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCgg                                                                                                                   >  1:5142219/1‑151 (MQ=255)
                                 ccgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCCGTCGGc                                                                                                                  >  2:7646738/1‑151 (MQ=255)
                                  cgGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCg                                                                                                                 >  1:8659865/1‑151 (MQ=255)
                                     ggcaTAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTAGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTAGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGACAGTCGGCGTtc                                                                                                              >  2:10330257/4‑151 (MQ=255)
                                      ggATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTtct                                                                                                             <  2:2586338/151‑1 (MQ=255)
                                       gATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGAATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGACGGCGTtctc                                                                                                            >  2:8199703/1‑151 (MQ=255)
                                        aTAAACCGGGGAAGGCCGGACACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcg                                                                                                           <  2:7043164/151‑1 (MQ=255)
                                         tAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcgc                                                                                                          >  2:6938380/1‑151 (MQ=255)
                                           caCCGGGGAAGGCCGGACACACTGTTCGCTGACGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTcgccg                                                                                                        <  2:9381095/150‑1 (MQ=255)
                                             ccGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGca                                                                                                      <  2:6060985/151‑1 (MQ=255)
                                                gggAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCGCAg                                                                                                   >  1:9672589/1‑151 (MQ=255)
                                                 ggAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAgg                                                                                                  >  2:4452719/1‑151 (MQ=255)
                                                   aaGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTc                                                                                                <  1:2039847/151‑1 (MQ=255)
                                                   aaGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGGTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTc                                                                                                >  1:8698944/1‑151 (MQ=255)
                                                    aGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCg                                                                                               >  1:9490606/1‑151 (MQ=255)
                                                        cGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAggg                                                                                           >  2:8033237/1‑151 (MQ=255)
                                                         ggAAACACTGGTCGCTGAAGCTGGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGc                                                                                          <  1:4750371/151‑1 (MQ=255)
                                                           aaaCACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCTGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGa                                                                                        >  2:1544711/1‑151 (MQ=255)
                                                           aaaCACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGa                                                                                        >  1:10749712/1‑151 (MQ=255)
                                                             acacTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAAc                                                                                      >  2:1248560/1‑151 (MQ=255)
                                                              cacTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAAcc                                                                                     >  1:8337065/1‑151 (MQ=255)
                                                               acTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCa                                                                                    >  1:1301675/1‑151 (MQ=255)
                                                               acTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCa                                                                                    >  1:8225479/1‑151 (MQ=255)
                                                                 tGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGc                                                                                  >  1:4042051/1‑151 (MQ=255)
                                                                   ttCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGt                                                                                <  2:3230785/151‑1 (MQ=255)
                                                                     cGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTg                                                                              <  2:2821173/151‑1 (MQ=255)
                                                                      gCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTgc                                                                             >  1:5869609/1‑151 (MQ=255)
                                                                       cTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTgcg                                                                            >  1:6745113/1‑151 (MQ=255)
                                                                         gAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCCGCGTTGCGCt                                                                          >  1:10001022/1‑151 (MQ=255)
                                                                         gAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCt                                                                          >  2:2480281/1‑151 (MQ=255)
                                                                          aagcaggACGCTGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCtt                                                                         <  2:10181663/145‑1 (MQ=255)
                                                                          aaGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCtt                                                                         >  1:84583/1‑151 (MQ=255)
                                                                           aGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtc                                                                        >  1:6915545/1‑151 (MQ=255)
                                                                             cTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtcgt                                                                      <  2:4406559/151‑1 (MQ=255)
                                                                              ttGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtcgtc                                                                     <  2:6225766/151‑1 (MQ=255)
                                                                              ttGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtcgtc                                                                     <  1:5802963/151‑1 (MQ=255)
                                                                               tGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTtcgtcg                                                                    >  2:7811852/1‑151 (MQ=255)
                                                                                gACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTcgc                                                                   <  1:7402025/151‑1 (MQ=255)
                                                                                  cGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGt                                                                 >  1:3403189/1‑151 (MQ=255)
                                                                                   gATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTAACGCGGTGTGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCTCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTc                                                                >  1:3528725/1‑151 (MQ=255)
                                                                                    aTGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTcc                                                               >  1:1372091/1‑151 (MQ=255)
                                                                                      gggCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGa                                                             <  2:8343333/151‑1 (MQ=255)
                                                                                       ggCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAg                                                            <  2:5820190/151‑1 (MQ=255)
                                                                                       ggCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAg                                                            >  2:9561666/1‑151 (MQ=255)
                                                                                          gcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGcc                                                         <  2:465853/151‑1 (MQ=255)
                                                                                          gcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGcc                                                         >  1:2464802/1‑151 (MQ=255)
                                                                                          gcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGcc                                                         <  1:3756281/151‑1 (MQ=255)
                                                                                          gcCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGcc                                                         >  2:7434309/1‑151 (MQ=255)
                                                                                           ccGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCg                                                        >  2:9111717/1‑151 (MQ=255)
                                                                                             gggCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGgc                                                      >  2:8783570/1‑151 (MQ=255)
                                                                                              ggCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGgcg                                                     >  1:10536901/1‑151 (MQ=255)
                                                                                                 aCGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCg                                                  >  2:5745380/1‑151 (MQ=255)
                                                                                                   gggcgggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAg                                                <  1:6038632/151‑1 (MQ=255)
                                                                                                    ggcgggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGTAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGc                                               <  2:10732166/151‑1 (MQ=255)
                                                                                                    ggcgggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGc                                               <  2:5292834/151‑1 (MQ=255)
                                                                                                    ggcgggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGc                                               <  1:2275655/151‑1 (MQ=255)
                                                                                                        ggcCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGc                                           >  2:8580671/1‑151 (MQ=255)
                                                                                                         gccgccGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGcc                                          >  2:10615004/1‑151 (MQ=255)
                                                                                                         gccgccGGCGTTGCCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGcc                                          <  2:1815665/151‑1 (MQ=255)
                                                                                                          ccgccgGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCg                                         >  1:4487289/1‑151 (MQ=255)
                                                                                                           cgccgGCGTTGTCGCGGATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGGTCTCGCCGCACAGGTCGAGGGCGACCCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGa                                        >  2:10758798/1‑151 (MQ=255)
                                                                                                            gccgGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGaa                                       <  2:2045617/151‑1 (MQ=255)
                                                                                                              cgGCGTTGTCGCGTATCGCGGGGAGCTGGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCTGGGCGGTCAGTCGGCGTTCTCGCCGCACTGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAaga                                     >  2:5140578/1‑151 (MQ=255)
                                                                                                              cgGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAaga                                     >  1:854524/1‑151 (MQ=255)
                                                                                                                gCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGc                                   <  2:10045657/151‑1 (MQ=255)
                                                                                                                gCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGc                                   >  1:7659039/1‑151 (MQ=255)
                                                                                                                  gTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCtt                                 >  1:2982791/1‑151 (MQ=255)
                                                                                                                    tGTCGCGTATCGCGGGGAGCTCGGAAGTAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGc                               >  2:4863837/1‑151 (MQ=255)
                                                                                                                      tCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCTGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGccc                             <  1:10137097/151‑1 (MQ=255)
                                                                                                                      tCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGccc                             >  1:7769116/1‑151 (MQ=255)
                                                                                                                      tCGCGTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGccc                             >  2:9706803/1‑151 (MQ=255)
                                                                                                                         cgTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAgg                          <  1:10379075/151‑1 (MQ=255)
                                                                                                                          gTATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAggg                         >  1:1714957/1‑151 (MQ=255)
                                                                                                                           tATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgc                        >  1:8866387/1‑151 (MQ=255)
                                                                                                                           tATCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgc                        >  1:5642459/1‑151 (MQ=255)
                                                                                                                            aTCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgcc                       >  2:1043382/1‑151 (MQ=255)
                                                                                                                            aTCGCGGGGAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGgcc                       >  1:5289443/1‑151 (MQ=255)
                                                                                                                                 ggggAGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTc                  <  2:3453678/151‑1 (MQ=255)
                                                                                                                                     aGCTCGGATGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGTGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCgg              <  2:1720888/151‑1 (MQ=255)
                                                                                                                                     aGCTCGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCgg              >  1:4269814/1‑151 (MQ=255)
                                                                                                                                        gcGGAAGGAAGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTc           <  1:10268715/150‑1 (MQ=255)
                                                                                                                                          ggaaggaaGCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCAt         >  1:6296020/1‑151 (MQ=255)
                                                                                                                                                aagCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTccacc   <  2:661692/151‑1 (MQ=255)
                                                                                                                                                 agCGG‑CCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTccacca  <  2:1992638/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GGCAGCGGAAGGCGACATCCTGTGGACTCGCCGCCGGGGGATAAACCGGGGAAGGCCGGAAACACTGTTCGCTGAAGCTTGACGATGGGCGCCGGGCACGGGCGGGCCGCCGGCGTTGTCGCGTATCGCGGGGAGCTCGGAAGGAAGCGGCCCGCCGGCGGGCGGCCGCAGGGCGGTCAGTCGGCGTTCTCGCCGCACAGGTCGAGGGCGAACCAGCGTTGCGCTTCGTCGCGTCCGAGGCCGGCGCCGAGCAGGCCGAAGAGCTTGCCCAGGGCCGCCTCGCGGGTCATTCCACCA  >  NC_008463/3150835‑3151131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑