BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	155,993	Δ1 bp	coding (922/981 nt)	PA14_RS00700 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	155,990	0	G	.	99.8%	2430.7 / ‑2.2	492	coding (919/981 nt)	PA14_RS00700	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (147/344); total (147/345)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 505 total aligned reads displayed.

GTGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAACAC  >  NC_008463/155840‑156136

gtggCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCg                                                                                                                                                    <  2:4192748/151‑1 (MQ=255)

 tggCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCgg                                                                                                                                                   <  2:10135925/151‑1 (MQ=255)

        ggggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGa                                                                                                                                           >  1:7937276/1‑151 (MQ=255)

         gggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGAc                                                                                                                                          <  2:10595777/151‑1 (MQ=255)

          ggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACg                                                                                                                                         <  1:2205216/151‑1 (MQ=255)

          ggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACg                                                                                                                                         <  1:2552233/151‑1 (MQ=255)

                ggCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCa                                                                                                                                   >  1:10243836/1‑151 (MQ=255)

                ggCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCa                                                                                                                                   <  2:2064987/151‑1 (MQ=255)

                 gCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAg                                                                                                                                  >  1:7305769/1‑151 (MQ=255)

                   tGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGt                                                                                                                                >  1:5905220/1‑151 (MQ=255)

                   tGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGt                                                                                                                                >  2:3091958/1‑151 (MQ=255)

                   tGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGt                                                                                                                                >  2:8524064/1‑151 (MQ=255)

                   tGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGt                                                                                                                                <  1:3982629/151‑1 (MQ=255)

                   tGTCGAGTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGt                                                                                                                                <  1:4444145/151‑1 (MQ=255)

                    gTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGtt                                                                                                                               >  1:10704394/1‑151 (MQ=255)

                    gTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGtt                                                                                                                               <  1:1502202/151‑1 (MQ=255)

                      cGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttct                                                                                                                             >  1:10397085/1‑151 (MQ=255)

                       gATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttctt                                                                                                                            >  1:7589295/1‑151 (MQ=255)

                        aTTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttcttc                                                                                                                           <  1:1093923/151‑1 (MQ=255)

                        aTTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttcttc                                                                                                                           <  1:9932267/151‑1 (MQ=255)

                           ccGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAt                                                                                                                        <  1:8521638/151‑1 (MQ=255)

                           ccGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAt                                                                                                                        <  1:9432358/151‑1 (MQ=255)

                             ggCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTa                                                                                                                      <  2:6827069/151‑1 (MQ=255)

                             ggCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTa                                                                                                                      >  2:9201667/1‑151 (MQ=255)

                             ggCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTa                                                                                                                      <  1:5491082/151‑1 (MQ=255)

                              gCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAg                                                                                                                     <  2:2551652/151‑1 (MQ=255)

                              gCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAg                                                                                                                     <  1:10025231/151‑1 (MQ=255)

                              gCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAg                                                                                                                     <  1:3029732/151‑1 (MQ=255)

                               ccAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgt                                                                                                                    <  2:2240578/151‑1 (MQ=255)

                               ccAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgt                                                                                                                    <  2:4112118/151‑1 (MQ=255)

                                  aCCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttgt                                                                                                                 <  2:3739986/151‑1 (MQ=255)

                                   ccTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttgtt                                                                                                                <  2:6486787/151‑1 (MQ=255)

                                   ccTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttgtt                                                                                                                <  1:8402521/151‑1 (MQ=255)

                                   ccTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttgtt                                                                                                                <  1:7017339/151‑1 (MQ=255)

                                   ccTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttgtt                                                                                                                <  2:7271216/151‑1 (MQ=255)

                                     tgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGtttt                                                                                                              <  2:9274902/151‑1 (MQ=255)

                                       ctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTaa                                                                                                            >  1:6814111/1‑151 (MQ=255)

                                         ggCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACt                                                                                                          <  2:9380967/151‑1 (MQ=255)

                                          gCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTa                                                                                                         <  2:4597763/151‑1 (MQ=255)

                                           cGATGCTGCCCTAAGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAt                                                                                                        >  2:1991490/1‑151 (MQ=255)

                                            gATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAtt                                                                                                       <  2:10689554/151‑1 (MQ=255)

                                             aTGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAttt                                                                                                      <  2:10505762/151‑1 (MQ=255)

                                             aTGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAttt                                                                                                      <  1:3788726/151‑1 (MQ=255)

                                                ctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtata                                                                                                   <  1:9813002/151‑1 (MQ=255)

                                                ctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtata                                                                                                   <  2:5369144/151‑1 (MQ=255)

                                                 tgcCCTCTGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatat                                                                                                  <  2:3471190/151‑1 (MQ=255)

                                                 tgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatat                                                                                                  <  2:8968152/151‑1 (MQ=255)

                                                   cccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTatatat                                                                                                >  1:8825483/1‑151 (MQ=255)

                                                     cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGTGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATTttc                                                                                              <  2:701079/151‑1 (MQ=255)

                                                     cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttc                                                                                              <  2:7872744/151‑1 (MQ=255)

                                                     cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttc                                                                                              <  1:6215140/151‑1 (MQ=255)

                                                      tATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttct                                                                                             >  1:6399799/1‑151 (MQ=255)

                                                       aTGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttctt                                                                                            <  1:1742865/151‑1 (MQ=255)

                                                       aTGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttctt                                                                                            >  2:7485207/1‑151 (MQ=255)

                                                          cggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGa                                                                                         <  2:4816137/151‑1 (MQ=255)

                                                          cggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGa                                                                                         >  1:6701203/1‑151 (MQ=255)

                                                           ggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGAt                                                                                        <  1:2798272/151‑1 (MQ=255)

                                                           ggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGAt                                                                                        <  1:7909202/151‑1 (MQ=255)

                                                           ggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGAt                                                                                        <  2:8035623/151‑1 (MQ=255)

                                                            gcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATa                                                                                       <  1:2059250/151‑1 (MQ=255)

                                                             cgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAg                                                                                      <  1:5832117/151‑1 (MQ=255)

                                                              gACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGa                                                                                     >  2:7372340/1‑151 (MQ=255)

                                                               aCCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAt                                                                                    <  2:1747555/151‑1 (MQ=255)

                                                               aCCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAt                                                                                    >  1:4770755/1‑151 (MQ=255)

                                                                ccATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAtt                                                                                   <  1:7118000/151‑1 (MQ=255)

                                                                 cATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAttt                                                                                  <  1:8244565/151‑1 (MQ=255)

                                                                     cTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGAt                                                                              <  1:9660615/151‑1 (MQ=255)

                                                                      tGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATa                                                                             <  2:8753871/151‑1 (MQ=255)

                                                                       ggTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATaa                                                                            <  1:7409237/151‑1 (MQ=255)

                                                                          gCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTAGTTTTAACTATTTATATATTATTCGATAGATTTAGATAAttt                                                                         >  2:6706807/1‑151 (MQ=255)

                                                                               tCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTg                                                                    <  1:1226831/151‑1 (MQ=255)

                                                                                 cTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGAt                                                                  <  1:9181331/151‑1 (MQ=255)

                                                                                  tGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATg                                                                 <  1:4145030/151‑1 (MQ=255)

                                                                                   gCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTGGTGTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCAATGATgg                                                                >  1:4593322/1‑151 (MQ=255)

                                                                                     tctcCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGGTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGcc                                                              >  2:6054801/1‑151 (MQ=255)

                                                                                          cgcgATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAAt                                                         <  2:5206809/151‑1 (MQ=255)

                                                                                            cgATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCa                                                       <  2:1277418/151‑1 (MQ=255)

                                                                                             gATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCaa                                                      >  1:1911453/1‑151 (MQ=255)

                                                                                               tGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGGGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATg                                                    >  2:5926125/1‑151 (MQ=255)

                                                                                                gCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATgg                                                   <  2:8378142/151‑1 (MQ=255)

                                                                                                      cgcACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAtt                                             >  1:4350170/1‑151 (MQ=255)

                                                                                                       gcACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAttt                                            <  2:4487756/151‑1 (MQ=255)

                                                                                                          cccGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTg                                         <  2:377767/151‑1 (MQ=255)

                                                                                                            cGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGcc                                       <  1:3694411/151‑1 (MQ=255)

                                                                                                            cGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTACATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGcc                                       <  2:6573669/151‑1 (MQ=255)

                                                                                                             ggCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCg                                      <  2:2986606/151‑1 (MQ=255)

                                                                                                                      ccccGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTTGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGtt                             <  1:10623925/151‑1 (MQ=255)

                                                                                                                       cccGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTa                            >  2:2689090/1‑151 (MQ=255)

                                                                                                                        ccGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTaa                           <  1:5217588/151‑1 (MQ=255)

                                                                                                                         cGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAg                          <  1:3535140/151‑1 (MQ=255)

                                                                                                                         cGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAg                          <  2:3243647/151‑1 (MQ=255)

                                                                                                                                  tGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCa                 <  2:4374789/151‑1 (MQ=255)

                                                                                                                                    ggCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGt               <  2:5043654/151‑1 (MQ=255)

                                                                                                                                      ccAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCg             <  2:8848083/151‑1 (MQ=255)

                                                                                                                                       cAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGa            >  1:6959252/1‑151 (MQ=255)

                                                                                                                                         gCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTTTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGAtt          <  2:9031405/151‑1 (MQ=255)

                                                                                                                                         gCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGAtt          <  2:9589418/151‑1 (MQ=255)

                                                                                                                                             tGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGaa      <  2:8167662/151‑1 (MQ=255)

                                                                                                                                                 aGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAacac  <  2:6244953/151‑1 (MQ=255)

                                                                                                                                                 aGGCC‑GGGCACTGACGAGGGCAGGTTCTACGATTAGTTGTGTTAACTAGTTATATATTCATCGATAGATTTAGAAAATTTCACTGATGGCCTAAATCAATGAGGCATTTTTGCAGAACACACATAAGATTCTCACAGTCGATTTGAAacac  >  2:5556299/1‑151 (MQ=255)

GTGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAACAC  >  NC_008463/155840‑156136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`