Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,819,717 (C)5→4 intergenic (‑115/+55) PA14_RS22115 ← / ← lepB DUF4845 domain‑containing protein/signal peptidase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,819,7130C.97.5% 1721.7 / 24.6 365intergenic (‑111/+59)PA14_RS22115/lepBDUF4845 domain‑containing protein/signal peptidase I
Reads supporting (aligned to +/- strand):  ref base C (1/8);  new base . (185/171);  total (186/179)
Fisher's exact test for biased strand distribution p-value = 1.80e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 392 total aligned reads displayed.

CCAGCAGGGACATCCCCTTCTGCGAACGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAGCCCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAGGCCTTGCCG  >  NC_008463/4819563‑4819859
                                                                                                                                                      |                                                                                                                                                  
ccAGCAGGGACATCCCCTTCTGCGAACGCGCGAACCTCATGTCGGACCTCTTGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGACCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGagcagc                                                                                                                                                    >  1:4359668/1‑151 (MQ=255)
 cagcagGGACATCCCCTTCTGCGAACGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAGcc                                                                                                                                                   <  1:2125741/151‑1 (MQ=255)
  agcagGGACATCCCCTTCTGCGAACGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAGccc                                                                                                                                                  <  1:8132770/151‑1 (MQ=255)
   gcagGGACATCCCCTTCTGCGAACGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAGcccc                                                                                                                                                 <  2:600713/151‑1 (MQ=255)
     agGGACATCCCCTTCTGCGAACGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAGccccgc                                                                                                                                               >  1:1131716/1‑149 (MQ=255)
          cATCCCCTTCTGCGACCGCGCGAACCTCAGGTCGGACCTCTTGTCTGTGTTGTTATTCCGTCCAGCGTGCGTTAACGCCATCGGGCAGGGCCCGGCGGCGGCGTTCGCGGAAGTTAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTcgc                                                                                                                                         <  2:689977/151‑1 (MQ=255)
          cATCCCCTTAGGCGAACGCGCGACCCTCATGGCGGACCTCTGGTCTGTGTTGTTTTTCCGCCCAGCGAGCGGTACCGCCATCGGGCTGGGCCCTGCGGCGGCGTTCGCGGATGTGAAAGCGGGGCTGCCCGGGGGAGCAG‑CCCCGCTTcgc                                                                                                                                         <  2:7961502/151‑1 (MQ=255)
              cccTTCTGCGAACGCGCTCACCACCTGTCGGACCTCATGTCCGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGGGGCGTTCGCGGAAGTTAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGatt                                                                                                                                     <  2:1627832/151‑1 (MQ=255)
                cTTCTGCTAACGCGCGAACCTCATGTCGGACCTATTGTCTGTGTTGTTATTCCGTCCTGCGAGCGTTACCGCCATCGGGCTGGGCCCTGCGGGGGCGTTCGCGGATGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGattat                                                                                                                                   <  2:1175753/151‑1 (MQ=255)
                cTTCTGCGAACGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGattat                                                                                                                                   >  1:10639071/1‑151 (MQ=255)
                 ttCTGCGAACGCGCGAACCTCATGTCGGACCTCAGGTCTGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCGGCCCTCGGGAGCAG‑CCCCGCTTCGCGattatt                                                                                                                                  <  2:9655154/151‑1 (MQ=255)
                     gCGAACGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCGTCGCGATTATTcagc                                                                                                                              >  2:8914279/1‑151 (MQ=255)
                      cGAACGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTcagca                                                                                                                             >  1:8926875/1‑151 (MQ=255)
                       gAACGCGCGAACCTCAGTTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGGGGCGGGGTTCGCGGAAGTGAAAGCGGGGCGGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTcagcag                                                                                                                            <  2:4842575/151‑1 (MQ=255)
                        aaCGCGCGAACCTCATGTCGGACCTCATGTCAGTTTATTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGTAAGTGAACGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAgg                                                                                                                           <  2:7314714/151‑1 (MQ=255)
                        aaCGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAgg                                                                                                                           <  2:5866714/151‑1 (MQ=255)
                        aaCGCGCGAACCACATGCCGTACCTCATGTCCGTGTAGTTATGCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCTG‑CCCCGCTTCGCGATTATTCAGCAgg                                                                                                                           <  2:6486027/151‑1 (MQ=255)
                               gAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGccc                                                                                                                    >  1:7527683/1‑151 (MQ=255)
                               gAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGccc                                                                                                                    >  2:8496166/1‑151 (MQ=255)
                               gAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGccc                                                                                                                    >  1:1251353/1‑151 (MQ=255)
                                aaCCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCt                                                                                                                   >  1:1703075/1‑151 (MQ=255)
                                 aCCTCATGTCGCACCTCAGGTCTGTGTTGTTATTCCGTCCAGCGAGCGGTACCGCCTTCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAATGCGGGGCTGCCCTCGGGAGCTG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCtt                                                                                                                  <  2:148681/151‑1 (MQ=255)
                                      aTGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCcgcg                                                                                                             >  2:5358932/1‑151 (MQ=255)
                                       tGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCcgcgg                                                                                                            >  2:2119000/1‑150 (MQ=255)
                                        gTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTg                                                                                                           >  2:8103798/1‑151 (MQ=255)
                                                tcttttCAGTTTATTTAGTCCGTCCCGCGTGCGGAAACGCCATCGGGCTGGGCCGGGCGGCGGCTTTCGCGGAAGTGAACGCGGGGCGGCCCTCGGGAGCA‑CCCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCt                                                                                                   <  1:2079542/146‑1 (MQ=255)
                                                tcttGTCTGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATGGGGCTGGGCCCTGCGGCGGCGTTCGCGTAAGTTAACGGGGGGCGCCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCt                                                                                                   <  1:6887319/148‑1 (MQ=255)
                                                tCATGTCAGGCTAGTGTTACCGTCCAGCGAGCGGTAACGCCATCGGGCCGGGCCCTGCGGCGGCGTACGCGGACGTGACCGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTCGTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCt                                                                                                   <  2:6082885/151‑1 (MQ=255)
                                                  aTGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTgc                                                                                                 >  1:3356560/1‑151 (MQ=255)
                                                  aTGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTgc                                                                                                 >  2:4077095/1‑151 (MQ=255)
                                                    gTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTgcgc                                                                                               >  1:3725339/1‑151 (MQ=255)
                                                    gTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCGTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTgcgc                                                                                               >  1:8289484/1‑151 (MQ=255)
                                                     tCAGTGTAGTTATTCCGTCCAGCGCGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGca                                                                                              <  2:2972282/151‑1 (MQ=255)
                                                      ccgtgtAGTTAGTCCGTCCCGCGAGCGGAAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGGGGAAGTGAAAGGGGGGCGGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGcac                                                                                             <  1:10273557/149‑1 (MQ=255)
                                                      cAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAATGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGcac                                                                                             <  2:3007427/151‑1 (MQ=255)
                                                      cAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGcac                                                                                             >  2:5607677/1‑151 (MQ=255)
                                                       tgttttgTTATTCCGTCCTGGGCGCGTTACCGCAAGGGGGCTGGGCCCGGGGGCGGGGTTCGCGGTTGTGAAGGGGGGGCGGCCCTGGGGAGCA‑CCCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGcaca                                                                                            <  1:4557638/145‑1 (MQ=255)
                                                        gtgtAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAg                                                                                           >  1:6411165/1‑151 (MQ=255)
                                                          gttgTTATTCCGTCCAGCGCGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAACGGGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGt                                                                                         <  1:8605656/148‑1 (MQ=255)
                                                          gtAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGt                                                                                         <  2:4190711/151‑1 (MQ=255)
                                                            aGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCa                                                                                       >  1:1881332/1‑151 (MQ=255)
                                                             gTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAg                                                                                      >  2:1234270/1‑151 (MQ=255)
                                                                    cGTCCAGCGAGCGGTAACGCCTTCGGGCAGGGCCCTGCGGCGGCGTTCGGGGAAGTGAAAGCGGGGGTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCa                                                                               <  2:7804024/151‑1 (MQ=255)
                                                                     gTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCAc                                                                              <  2:10417263/151‑1 (MQ=255)
                                                                     gTCCACTGACCGGTATCGCCATCGGGAAGCGCCCTGCGGCGGAGTTCGCGTAAGTGAAAGCGGGGCTGCCCTGGGGACCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCAc                                                                              <  2:3327989/151‑1 (MQ=255)
                                                                         agcgagcgGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAACGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCg                                                                          <  2:281995/151‑1 (MQ=255)
                                                                         agcgagcgGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCg                                                                          <  2:8631711/151‑1 (MQ=255)
                                                                           cgagcgGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGAc                                                                        <  2:2010654/151‑1 (MQ=255)
                                                                            gagcgGTAACGCCCTCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGCGCAG‑CCCCGCTTGGCGATTATGAAGCCGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGAcc                                                                       >  2:9930840/1‑151 (MQ=255)
                                                                            gagcgGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGAcc                                                                       >  1:5359988/1‑151 (MQ=255)
                                                                               cgGTAACGCCATCGGGCAGGGCCATGCGGCGGCGTTCGAGGAAGTGAATCCGGGGCTGCCCTCGGGAGCAG‑CCCCTCGTCGCGATTATTAAGCTGGAGCGCCCTTCCGCGAGGCGAACCAGCGGACAGGTCAGTGAATCACGCCGACCCgg                                                                    >  2:6223438/1‑151 (MQ=255)
                                                                                ggTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCggg                                                                   <  2:7601653/151‑1 (MQ=255)
                                                                                ggTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCggg                                                                   >  2:4640730/1‑151 (MQ=255)
                                                                                 gTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGga                                                                  >  2:6708137/1‑151 (MQ=255)
                                                                                   aaCGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGgaga                                                                <  2:132965/151‑1 (MQ=255)
                                                                                    aCGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGaa                                                               >  2:5288612/1‑151 (MQ=255)
                                                                                      gCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGt                                                             >  1:4905565/1‑151 (MQ=255)
                                                                                       ccATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGtg                                                            >  2:10205670/1‑150 (MQ=255)
                                                                                        cATCGGGCCGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTc                                                           >  1:6032712/1‑151 (MQ=255)
                                                                                         aTCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCGTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCg                                                          >  1:2938578/1‑151 (MQ=255)
                                                                                          tCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCgg                                                         >  1:5273152/1‑151 (MQ=255)
                                                                                           cGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGc                                                        >  1:1978001/1‑151 (MQ=255)
                                                                                            gggCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCa                                                       <  2:2422595/151‑1 (MQ=255)
                                                                                            gggCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCa                                                       >  1:8501410/1‑151 (MQ=255)
                                                                                                aGGGCCCTGCGGCGGCGTTCGCGGATGTTAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGtt                                                   <  1:3932921/151‑1 (MQ=255)
                                                                                                  ggCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGCCCCGGGAGAAGTTCGGCAGGTTGc                                                 >  1:1039795/1‑151 (MQ=255)
                                                                                                   gCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCCGGTTGCt                                                >  2:7060053/1‑151 (MQ=255)
                                                                                                    cccTGCGGCGGCGTTCGCGTAAGTGAAAGCGGGGCTGCCCGCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTc                                               <  1:9713603/151‑1 (MQ=255)
                                                                                                      cggcggcggcgTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCAt                                             <  1:8773730/149‑1 (MQ=255)
                                                                                                        gcggcggcgTTCGCGGAAGTGAAAGCGGGGCGGCCCTCGGGACCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCt                                           <  1:5749759/151‑1 (MQ=255)
                                                                                                            cggcgTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCgg                                       >  2:5468723/1‑151 (MQ=255)
                                                                                                              gcgTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGAt                                     <  2:3187753/151‑1 (MQ=255)
                                                                                                                gTTCGCGTAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCg                                   <  2:4411511/151‑1 (MQ=255)
                                                                                                                gTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCg                                   >  1:9388458/1‑151 (MQ=255)
                                                                                                                gTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCg                                   >  2:1815784/1‑151 (MQ=255)
                                                                                                                 ttCGCGTAAGTGAACGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCgg                                  <  1:2849681/151‑1 (MQ=255)
                                                                                                                     cgGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCa                              <  2:9118399/151‑1 (MQ=255)
                                                                                                                     cgGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCa                              <  2:2623767/151‑1 (MQ=255)
                                                                                                                      ggAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAg                             >  1:2312202/1‑151 (MQ=255)
                                                                                                                       gAAGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGc                            <  2:10736210/151‑1 (MQ=255)
                                                                                                                        aaGTGAACGCGGGGCGGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACATGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCt                           <  1:5836821/151‑1 (MQ=255)
                                                                                                                        aaGTGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCt                           <  2:8374956/151‑1 (MQ=255)
                                                                                                                         aGTGAAAGCGGGGCTCCCCGCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTc                          <  1:10075377/151‑1 (MQ=255)
                                                                                                                           tGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCAt                        <  2:5074136/151‑1 (MQ=255)
                                                                                                                           tGAAAGCGGGGCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCAt                        >  2:4513478/1‑151 (MQ=255)
                                                                                                                                  ggggCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACg                 >  1:7148359/1‑151 (MQ=255)
                                                                                                                                   gggCTGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACgg                >  1:7321439/1‑151 (MQ=255)
                                                                                                                                       tGCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGaa            <  1:6672763/151‑1 (MQ=255)
                                                                                                                                        gCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAg           <  2:376127/151‑1 (MQ=255)
                                                                                                                                        gCCCTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAg           >  1:9533885/1‑151 (MQ=255)
                                                                                                                                         cccTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAgg          <  2:3476470/151‑1 (MQ=255)
                                                                                                                                         cccTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAgg          <  1:4122126/151‑1 (MQ=255)
                                                                                                                                         cccTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAgg          <  2:9844729/151‑1 (MQ=255)
                                                                                                                                          ccTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAGGc         >  1:3205963/1‑151 (MQ=255)
                                                                                                                                           cTCGGGAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAGGcc        <  2:9368980/151‑1 (MQ=255)
                                                                                                                                               ggAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAGGCCTTGc    <  2:1065050/151‑1 (MQ=255)
                                                                                                                                               ggAGCAG‑CCCCGCGTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAGTCCTTGc    >  2:8720935/1‑151 (MQ=255)
                                                                                                                                               gcagcag‑cCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAGGCCTTGc    <  1:7676833/149‑1 (MQ=255)
                                                                                                                                                gAGCAG‑CCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAGGCCTTGcc   <  2:270997/151‑1 (MQ=255)
                                                                                                                                                 agcag‑cCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAGGCCTTGCcg  >  1:8197231/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CCAGCAGGGACATCCCCTTCTGCGAACGCGCGAACCTCATGTCGGACCTCATGTCAGTGTAGTTATTCCGTCCAGCGAGCGGTAACGCCATCGGGCAGGGCCCTGCGGCGGCGTTCGCGGAAGTGAAAGCGGGGCTGCCCTCGGGAGCAGCCCCCGCTTCGCGATTATTCAGCAGGAGCGCCCTTCCGCGTGGCGAACCTGCGCACAGGTCAGTGAATCACGCCGACCCGGGAGAAGTTCGGCAGGTTGCTCATCTTCGGATCGGGCCAGCTCATCCACACGGCGAAGGCCTTGCCG  >  NC_008463/4819563‑4819859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑