Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,358,513 Δ1 bp pseudogene (774/1285 nt) PA14_RS24540 → site‑specific integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,358,5100T.100.0% 533.7 / NA 261pseudogene (771/1285 nt)PA14_RS24540site‑specific integrase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (127/134);  total (127/134)

Only 100 of 282 total aligned reads displayed.

CGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATG  >  NC_008463/5358360‑5358656
                                                                                                                                                      |                                                                                                                                                  
cGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGt                                                                                                                                                    >  2:3762001/1‑151 (MQ=31)
cGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGt                                                                                                                                                    <  1:151603/151‑1 (MQ=31)
cGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGt                                                                                                                                                    <  2:1104879/151‑1 (MQ=31)
 gATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtt                                                                                                                                                   >  2:2542826/1‑151 (MQ=31)
 gATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtt                                                                                                                                                   <  1:1474180/151‑1 (MQ=31)
 gATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtt                                                                                                                                                   >  1:286924/1‑151 (MQ=31)
   tAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGtttc                                                                                                                                                 <  1:6549642/151‑2 (MQ=14)
                                                    tcACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTc                                                                                               >  2:1160696/1‑151 (MQ=1)
                                                     cACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCa                                                                                              >  1:8174467/1‑151 (MQ=11)
                                                      aCCGCCCGACAAGAGCAAGGTGCTGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGGTGGGGTGCGGATGACCCCGCACCG‑TGTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCAc                                                                                             >  2:7186859/1‑151 (MQ=11)
                                                      aCCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTctc                                                                                             >  1:6830285/1‑149 (MQ=1)
                                                        cGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCt                                                                                           >  1:673676/1‑151 (MQ=11)
                                                         gCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTc                                                                                          >  1:862134/1‑151 (MQ=11)
                                                         gCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTc                                                                                          >  2:789922/1‑151 (MQ=11)
                                                         gCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTc                                                                                          <  2:8424004/151‑1 (MQ=11)
                                                          cACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCa                                                                                         <  2:3825777/151‑1 (MQ=11)
                                                          cACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCa                                                                                         >  2:1522444/1‑151 (MQ=11)
                                                          cACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCa                                                                                         <  2:6425937/151‑1 (MQ=11)
                                                           aCTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCAc                                                                                        <  1:2556745/151‑1 (MQ=11)
                                                            cTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACg                                                                                       >  1:215534/1‑151 (MQ=11)
                                                             tACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGCAGGTGGGGGTGCGGATGACCCCGCACCG‑TGTCCGGCCCACCCTGGCCACCGACGTGATGAAGGCCCCCGAGCGGAACATTCACCTCTCGa                                                                                      >  2:1789611/1‑151 (MQ=11)
                                                                aaGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGt                                                                                   <  1:2719677/151‑1 (MQ=11)
                                                                    gCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGACCCTTCACCTCACGAAGTGCCt                                                                               >  2:9578294/1‑151 (MQ=2)
                                                                      aaGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGc                                                                             <  2:602364/151‑1 (MQ=11)
                                                                      aaGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGc                                                                             <  2:7635549/151‑1 (MQ=11)
                                                                      aaGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGc                                                                             >  2:2739681/1‑151 (MQ=11)
                                                                       aGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCt                                                                            <  1:5985984/151‑1 (MQ=11)
                                                                         gTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCa                                                                          >  1:10118914/1‑151 (MQ=11)
                                                                            atgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAcc                                                                       <  2:4292274/151‑1 (MQ=11)
                                                                            atgaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAAcc                                                                       <  1:6051589/151‑1 (MQ=11)
                                                                              gaACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCAc                                                                     <  2:7461419/151‑1 (MQ=11)
                                                                               aaCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACt                                                                    >  1:9156188/1‑151 (MQ=11)
                                                                                aCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTc                                                                   >  1:8464551/1‑151 (MQ=11)
                                                                                aCTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTc                                                                   <  1:1735974/151‑1 (MQ=11)
                                                                                 cTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGGACATTCACCTCACGAAGTGCCTGCTCAAACACTCg                                                                  >  2:6949484/1‑151 (MQ=2)
                                                                                  tCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGa                                                                 <  1:6244942/151‑1 (MQ=11)
                                                                                     gACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAata                                                              >  1:6973389/1‑151 (MQ=11)
                                                                                      aCCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAatat                                                             >  2:6365222/1‑151 (MQ=11)
                                                                                        cAGGTCGAAGCCATGTACCGGAATGTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGACTATcc                                                           <  2:5485561/151‑1 (MQ=2)
                                                                                         aGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                          >  2:2246862/1‑151 (MQ=11)
                                                                                         aGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                          >  2:10588172/1‑151 (MQ=11)
                                                                                         aGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                          >  2:7868417/1‑151 (MQ=11)
                                                                                         aGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                          >  1:3805233/1‑151 (MQ=11)
                                                                                         aGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCa                                                          >  1:5048720/1‑151 (MQ=11)
                                                                                           gTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGa                                                        >  2:4668641/1‑151 (MQ=11)
                                                                                           gTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGa                                                        <  1:10314000/151‑1 (MQ=11)
                                                                                            tCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAc                                                       >  1:7829459/1‑151 (MQ=11)
                                                                                            tCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAc                                                       <  1:3241222/151‑1 (MQ=11)
                                                                                             cGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGAcc                                                      <  2:9759603/151‑1 (MQ=11)
                                                                                              gAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCa                                                     <  1:2399810/151‑1 (MQ=11)
                                                                                              gAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCa                                                     >  2:8743431/1‑151 (MQ=11)
                                                                                              gAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCa                                                     <  1:6102132/151‑1 (MQ=11)
                                                                                               aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCAc                                                    >  1:7697213/1‑151 (MQ=11)
                                                                                               aaGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCAc                                                    >  2:8882561/1‑151 (MQ=11)
                                                                                                 gCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCAcca                                                  <  1:2132235/151‑3 (MQ=11)
                                                                                                 gCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGa                                                  <  2:9150103/151‑1 (MQ=14)
                                                                                                  ccATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGAt                                                 <  1:1875896/151‑1 (MQ=17)
                                                                                                    aTGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGa                                               >  2:2893674/1‑151 (MQ=17)
                                                                                                       tACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCt                                            >  2:6070454/1‑151 (MQ=17)
                                                                                                        aCCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCCCTCGAATATCCAGACCACGATGAGCTa                                           >  2:5889369/1‑151 (MQ=12)
                                                                                                           ggAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACAt                                        >  1:7372738/1‑151 (MQ=17)
                                                                                                           ggAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACAt                                        <  1:4397765/151‑1 (MQ=17)
                                                                                                            gAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATc                                       <  2:9271558/151‑1 (MQ=17)
                                                                                                            gAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATc                                       <  2:7485927/151‑1 (MQ=17)
                                                                                                             aaGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCg                                      <  1:5893491/151‑1 (MQ=17)
                                                                                                             aaGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCg                                      <  2:8142761/151‑1 (MQ=17)
                                                                                                              aGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGa                                     <  1:5154638/151‑1 (MQ=17)
                                                                                                               gTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAg                                    <  2:8277989/151‑1 (MQ=17)
                                                                                                                ttGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAgg                                   <  1:7985742/151‑1 (MQ=17)
                                                                                                                   aCCGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCg                                >  1:4697275/1‑151 (MQ=17)
                                                                                                                    ccGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGa                               <  1:3524589/151‑1 (MQ=17)
                                                                                                                     cGAGAAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGAc                              >  1:8987946/1‑151 (MQ=17)
                                                                                                                      gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             >  2:10681771/1‑151 (MQ=17)
                                                                                                                      gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             <  1:1577659/151‑1 (MQ=17)
                                                                                                                      gagaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACt                             >  2:3188815/1‑151 (MQ=17)
                                                                                                                       agaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTa                            >  1:3869202/1‑151 (MQ=17)
                                                                                                                        gaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTAc                           <  1:9873791/151‑1 (MQ=17)
                                                                                                                        gaAGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTAc                           <  2:6463741/151‑1 (MQ=17)
                                                                                                                         aaGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACg                          >  2:4731295/1‑151 (MQ=17)
                                                                                                                         aaGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACg                          >  2:8594855/1‑151 (MQ=17)
                                                                                                                          aGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGa                         >  2:5303823/1‑151 (MQ=17)
                                                                                                                          aGGTTGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGa                         >  2:2024377/1‑151 (MQ=17)
                                                                                                                              tGGGGTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATcac                     >  1:7293092/1‑151 (MQ=21)
                                                                                                                               ggggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATcaca                    >  1:1357866/1‑151 (MQ=21)
                                                                                                                                gggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACAt                   <  2:7043473/151‑1 (MQ=21)
                                                                                                                                gggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACAt                   <  2:5121873/151‑1 (MQ=21)
                                                                                                                                 ggTGCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATg                  <  1:2994822/151‑1 (MQ=21)
                                                                                                                                    gCGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgt               <  1:5611207/151‑1 (MQ=21)
                                                                                                                                     cGGATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtg              >  1:5391323/1‑151 (MQ=21)
                                                                                                                                      ggATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgc             >  2:10271346/1‑151 (MQ=21)
                                                                                                                                      ggATGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgc             >  1:5914913/1‑151 (MQ=21)
                                                                                                                                         tGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgccgt          <  2:5608781/151‑1 (MQ=21)
                                                                                                                                         tGACCCCGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGcgtgccgt          <  1:5309987/151‑1 (MQ=21)
                                                                                                                                            ccccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGtgct       <  2:4045593/151‑1 (MQ=18)
                                                                                                                                            ccccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGtgct       >  1:427450/1‑151 (MQ=18)
                                                                                                                                              ccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACTATCACATGCGTGCCGtgctgc     >  2:3706823/1‑151 (MQ=12)
                                                                                                                                              ccGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGtgctgc     >  1:3301136/1‑151 (MQ=17)
                                                                                                                                               cGCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCa    <  1:10707950/151‑1 (MQ=17)
                                                                                                                                                gCACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCAt   >  1:5436715/1‑151 (MQ=17)
                                                                                                                                                 cACCG‑TTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATg  >  1:7900568/1‑151 (MQ=17)
                                                                                                                                                      |                                                                                                                                                  
CGATAGAGCCGGATTCGCCGATGACGACCAGTTGTTCAACGTCAACCGGTTCTCACCGCACTACAAGAGCAAGGTGATGAACTCCGACCAGGTCGAAGCCATGTACCGGAAGTTGACCGAGAAGGTTGGGGTGCGGATGACCCCGCACCGTTTTCCGGCACACCCTGGCCACCGACTTGATGAAGGCACCCGAGCGGAACATTCACCTCACGAAGTGCCTGCTCAACCACTCGAATATCCAGACCACGATGAGCTACATCGAGGCCGACTACGATCACATGCGTGCCGTGCTGCATG  >  NC_008463/5358360‑5358656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑