Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,280,717 (C)6→5 intergenic (‑46/+41) PA14_RS06005 ← / ← PA14_RS06010 DUF4345 domain‑containing protein/M23 family metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,280,7120C.100.0% 2263.7 / NA 456intergenic (‑41/+46)PA14_RS06005/PA14_RS06010DUF4345 domain‑containing protein/M23 family metallopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (279/177);  total (279/177)

Only 100 of 496 total aligned reads displayed.

TCGCCGCTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGCCCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGTGGCGC  >  NC_008463/1280562‑1280858
                                                                                                                                                      |                                                                                                                                                  
 cgccgcTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGcc                                                                                                                                                   <  2:404235/151‑1 (MQ=255)
    cgcTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccc                                                                                                                                                >  1:1610265/1‑151 (MQ=255)
      cTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccctg                                                                                                                                              <  1:4220912/151‑3 (MQ=255)
      cTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccctg                                                                                                                                              <  2:9326188/151‑3 (MQ=255)
      cTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccctg                                                                                                                                              >  2:4366773/1‑149 (MQ=255)
       tGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccctgt                                                                                                                                             >  1:3321135/1‑148 (MQ=255)
       tGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccctgt                                                                                                                                             >  1:230620/1‑148 (MQ=255)
       tGGTGAGCAGCGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGccccctgt                                                                                                                                             <  2:7977337/151‑4 (MQ=255)
           gAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCt                                                                                                                                        >  2:3444202/1‑151 (MQ=255)
             gCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTc                                                                                                                                      <  2:3012412/151‑1 (MQ=255)
                    ggCGAGCGGGCCGGGGAAGAAGACGTCGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTc                                                                                                                               <  2:10435424/151‑1 (MQ=255)
                    ggCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTc                                                                                                                               >  2:10077139/1‑151 (MQ=255)
                       gAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGc                                                                                                                            >  2:5663946/1‑151 (MQ=255)
                        aGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGcc                                                                                                                           >  1:2988543/1‑151 (MQ=255)
                         gCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGCGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCg                                                                                                                          <  2:9641067/151‑1 (MQ=255)
                         gCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCg                                                                                                                          <  2:1097218/151‑1 (MQ=255)
                           gggCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGgc                                                                                                                        >  2:10458/1‑151 (MQ=255)
                             gCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGgcgc                                                                                                                      >  1:4472181/1‑151 (MQ=255)
                              ccGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGcgcc                                                                                                                     <  2:8624039/151‑1 (MQ=255)
                               cGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGcgccg                                                                                                                    >  1:10159305/1‑151 (MQ=255)
                               cGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGcgccg                                                                                                                    >  1:9343638/1‑151 (MQ=255)
                                 gggAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCcgcg                                                                                                                  >  2:9823867/1‑151 (MQ=255)
                                 gggAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCcgcg                                                                                                                  >  1:6774305/1‑151 (MQ=255)
                                   gaagaaGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGtt                                                                                                                >  2:8944025/1‑151 (MQ=255)
                                     agaagaCGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGc                                                                                                              <  1:2428971/151‑1 (MQ=255)
                                      gaagaCGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCt                                                                                                             >  2:1833998/1‑151 (MQ=255)
                                       aagaCGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTc                                                                                                            >  2:3195167/1‑151 (MQ=255)
                                       aagaCGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTc                                                                                                            >  1:6342133/1‑151 (MQ=255)
                                         gaCGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCAc                                                                                                          >  2:759596/1‑151 (MQ=255)
                                            gAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTtc                                                                                                       >  2:4900116/1‑151 (MQ=255)
                                             aaGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTtct                                                                                                      >  2:8735764/1‑151 (MQ=255)
                                              aGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTtctc                                                                                                     <  1:5005293/151‑1 (MQ=255)
                                               gCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCa                                                                                                    <  1:6167769/151‑1 (MQ=255)
                                                cccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAg                                                                                                   >  2:2887178/1‑151 (MQ=255)
                                                cccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAg                                                                                                   >  1:804118/1‑151 (MQ=255)
                                                cccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAg                                                                                                   <  1:4865463/151‑1 (MQ=255)
                                                 ccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCTGCCGGCGCCGCGGTGCTCACTTCTCAgg                                                                                                  >  1:1073491/1‑151 (MQ=255)
                                                 ccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAgg                                                                                                  <  2:10337694/151‑1 (MQ=255)
                                                 ccAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGGTGCTCACTTCTCAgg                                                                                                  >  2:1736219/1‑151 (MQ=255)
                                                  cAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAggg                                                                                                 <  1:2089696/151‑1 (MQ=255)
                                                   aGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGc                                                                                                >  1:9128648/1‑151 (MQ=255)
                                                    ggCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCt                                                                                               >  1:1273948/1‑151 (MQ=255)
                                                    ggCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCt                                                                                               <  1:10479377/151‑1 (MQ=255)
                                                     gCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTg                                                                                              <  1:1465035/151‑1 (MQ=255)
                                                      ccGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGGTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTgg                                                                                             >  2:5268498/1‑151 (MQ=255)
                                                       cGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTggt                                                                                            >  2:2079341/1‑150 (MQ=255)
                                                        gAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGaa                                                                                           >  2:2440695/1‑151 (MQ=255)
                                                         aGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAg                                                                                          <  2:5360135/151‑1 (MQ=255)
                                                           gACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGc                                                                                        >  1:9867833/1‑151 (MQ=255)
                                                           gACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGc                                                                                        <  2:6531516/151‑1 (MQ=255)
                                                            aCCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGACGGCa                                                                                       >  1:2868829/1‑151 (MQ=255)
                                                              cACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCACAGCCGGCGCCGCGTTGCTCACTTCACAGGGCTGGAAGGCAcc                                                                                     >  1:7317065/1‑151 (MQ=255)
                                                                cGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGa                                                                                   >  2:224172/1‑151 (MQ=255)
                                                                  aaCAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCCTCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATg                                                                                 >  2:4153787/1‑151 (MQ=255)
                                                                  aaCAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATg                                                                                 <  1:8792474/151‑1 (MQ=255)
                                                                  aaCAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATg                                                                                 <  2:7078655/151‑1 (MQ=255)
                                                                  aaCAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATg                                                                                 >  1:5309609/1‑151 (MQ=255)
                                                                     aGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAg                                                                              >  2:9235459/1‑151 (MQ=255)
                                                                      gTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGa                                                                             <  1:3967032/151‑1 (MQ=255)
                                                                       tGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGAt                                                                            >  2:1239711/1‑151 (MQ=255)
                                                                       tGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGAt                                                                            <  2:3657864/151‑1 (MQ=255)
                                                                        gCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATc                                                                           <  2:687700/151‑1 (MQ=255)
                                                                        gCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGGTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGGTGAAGATc                                                                           >  1:9578543/1‑151 (MQ=255)
                                                                         ccGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCg                                                                          >  1:7539263/1‑151 (MQ=255)
                                                                          cGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGc                                                                         >  1:8156114/1‑151 (MQ=255)
                                                                          cGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGGTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGc                                                                         >  1:5882004/1‑151 (MQ=255)
                                                                              cacaGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCggg                                                                     >  1:7789834/1‑151 (MQ=255)
                                                                              cacaGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCggg                                                                     <  2:6714970/151‑1 (MQ=255)
                                                                                 aGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCg                                                                  >  1:5147441/1‑151 (MQ=255)
                                                                                 aGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCg                                                                  <  2:8842820/151‑1 (MQ=255)
                                                                                 aGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCg                                                                  >  1:4668219/1‑151 (MQ=255)
                                                                                     agagCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGAccc                                                              >  1:769511/1‑151 (MQ=255)
                                                                                       agCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCcgc                                                            >  1:57938/1‑151 (MQ=255)
                                                                                          aGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCcgcgcg                                                         >  2:3878604/1‑151 (MQ=255)
                                                                                              gAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGcgtcgt                                                     >  2:5180827/1‑151 (MQ=255)
                                                                                               aaGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGtt                                                    >  2:6150528/1‑151 (MQ=255)
                                                                                                 gCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCa                                                  <  1:7430681/151‑1 (MQ=255)
                                                                                                  cGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAg                                                 >  1:1940106/1‑151 (MQ=255)
                                                                                                   gggTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAgg                                                <  1:6040734/151‑1 (MQ=255)
                                                                                                    ggTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGc                                               >  1:6512281/1‑151 (MQ=255)
                                                                                                      tGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTg                                             >  1:8438841/1‑151 (MQ=255)
                                                                                                          tCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGt                                         >  2:5899712/1‑151 (MQ=255)
                                                                                                          tCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGt                                         >  1:10054795/1‑151 (MQ=255)
                                                                                                             tGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCCGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTcc                                      <  2:7218444/151‑1 (MQ=255)
                                                                                                             tGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCACAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGATcc                                      >  2:1052779/1‑151 (MQ=255)
                                                                                                                 gtgtCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTg                                  >  2:3330498/1‑151 (MQ=255)
                                                                                                                  tgtCTCCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGc                                 >  1:5496843/1‑151 (MQ=255)
                                                                                                                     ctcCCTTGGTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCAtg                              <  1:3881362/151‑1 (MQ=255)
                                                                                                                         cTTGGTCATGTTTGGGGCGCATCTACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCGCACTTCTGAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGGCGTTCAGGCTGACGTTCCAGTGCATGGGCg                          <  2:505752/151‑1 (MQ=255)
                                                                                                                             gTCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTcc                      <  2:1370816/151‑1 (MQ=255)
                                                                                                                              tCATGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCg                     >  2:9088575/1‑151 (MQ=255)
                                                                                                                              tCATGAAAGGGGCGCATCCACCCG‑CCCCCAGTTCCTTCTGAACACAGCCGGCGCCCCGTTGCTCAATTCACAGGGCTTGAAGGCACCCAAGAAGATCGCCGGGTAGACCCGCGCCGCGTTCAGGCTGACGTTCCAGTGCATGTTCGGTCCg                     >  2:8242387/1‑151 (MQ=255)
                                                                                                                                 tGAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTg                  <  1:10423535/151‑1 (MQ=255)
                                                                                                                                  gAAAGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGGTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTgg                 <  2:5447600/151‑1 (MQ=255)
                                                                                                                                   aaaGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGc                >  1:2603259/1‑151 (MQ=255)
                                                                                                                                     aGGGGCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGccc              >  2:4523819/1‑151 (MQ=255)
                                                                                                                                      ggggCGCATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGGGCACTGCTCAGGGCTGGAAGGCACCGATGACGATCGCCGGGTCGACCCGCGCGACGTTCAGGCTGAGGTTCCTGTGCATGTGCGGTCCGGTGGCGCt             >  2:6392106/1‑151 (MQ=255)
                                                                                                                                            cATCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGt       <  1:3543504/151‑1 (MQ=255)
                                                                                                                                             aTCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGTg      <  2:6812052/151‑1 (MQ=255)
                                                                                                                                             aTCGACGCG‑CCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGTg      <  1:1780029/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
TCGCCGCTGGTGAGCAGGGAGGCGAGCGGGCCGGGGAAGAAGACGAAGCCCAGGCCGAGGACCACGAACAGTGCCGAGCACAGCCAGAGCAGGCGAAGCGGGTGAGTCATGCGGTGTCTCCCTTGGTCATGAAAGGGGCGCATCGACGCGCCCCCCTGTTCCTTCTGACCTCAGCCGGCGCCGCGTTGCTCACTTCTCAGGGCTGGAAGGCACCGATGAAGATCGCCGGGTCGACCCGCGCGTCGTTCAGGCTGACGTTCCAGTGCATGTGCGGTCCGGTGGCCCTGCCGGTGGCGC  >  NC_008463/1280562‑1280858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑