Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,441,861 (C)6→4 intergenic (+31/+11) mexK → / ← PA14_RS06750 multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,441,8570C.99.7% 1656.0 / ‑2.0 336intergenic (+27/+16)mexK/PA14_RS06750multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (143/192);  total (143/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
*NC_0084631,441,8580C.99.7% 1654.0 / ‑2.0 336intergenic (+28/+15)mexK/PA14_RS06750multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (143/192);  minor base G (0/1);  total (143/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 396 total aligned reads displayed.

CTTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGCCCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTCG  >  NC_008463/1441707‑1442005
                                                                                                                                                      ||                                                                                                                                                   
  tcGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccc                                                                                                                                                    <  1:2928631/151‑1 (MQ=255)
   cGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGcccc                                                                                                                                                   <  2:7807420/151‑1 (MQ=255)
   cGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGGTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGGGTGAAGAAGGCCGAGGCGCGGGCCTGACGGGAGATGGGGACGGGGCGATCGCGcccc                                                                                                                                                   >  2:6290595/1‑151 (MQ=255)
    gggCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccg                                                                                                                                                  >  2:4377112/1‑150 (MQ=255)
       ccGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtcc                                                                                                                                               <  1:4354695/151‑5 (MQ=255)
       ccGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtcc                                                                                                                                               <  1:1935356/151‑5 (MQ=255)
         gATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgt                                                                                                                                             >  2:1213691/1‑145 (MQ=255)
         gATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgt                                                                                                                                             <  2:4799255/151‑7 (MQ=255)
          aTGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgtc                                                                                                                                            <  1:4826424/151‑8 (MQ=255)
          aTGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgtc                                                                                                                                            <  2:130900/151‑8 (MQ=255)
          aTGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgtc                                                                                                                                            >  2:5309554/1‑144 (MQ=255)
            ggCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGa                                                                                                                                        <  1:2792578/151‑1 (MQ=255)
            ggCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGa                                                                                                                                        >  2:5018930/1‑151 (MQ=255)
              cGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACt                                                                                                                                      <  1:5612925/151‑1 (MQ=255)
                 tGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCAc                                                                                                                                   <  1:5991208/151‑1 (MQ=255)
                  ggCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGGGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCAcc                                                                                                                                  >  1:2143292/1‑151 (MQ=255)
                     gATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTgg                                                                                                                               >  1:9910225/1‑151 (MQ=255)
                      aTCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCAATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGc                                                                                                                              >  1:4484653/1‑151 (MQ=255)
                          tGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCcgcg                                                                                                                          <  2:2303104/151‑1 (MQ=255)
                            ggcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGACGGGGACGGGGCGATCGCG‑‑CCCCGTCCGGCGACTCCCCTGGCCGCGcc                                                                                                                        >  1:172562/1‑151 (MQ=255)
                            ggcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGcc                                                                                                                        >  2:8906583/1‑151 (MQ=255)
                             gcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCg                                                                                                                       <  1:3022026/151‑1 (MQ=255)
                              cgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGa                                                                                                                      >  2:1910624/1‑151 (MQ=255)
                                  cTGATCGTCGCCACCGTGATCACCCTGCTGTTCCTGGCGTCGCTCTATGCCTCCTGGTTCCGTGTGATGAAGGACGAGGGGAGTGCCTGACGGTAGAAGTGGAATGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCTCGCAGTAGAc                                                                                                                  <  2:10500633/151‑1 (MQ=255)
                                    gATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGTCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGACGACct                                                                                                                >  1:739300/1‑151 (MQ=255)
                                      tcgtcgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACcttc                                                                                                              <  2:2808663/151‑1 (MQ=255)
                                       cgtcgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACcttct                                                                                                             >  1:795165/1‑151 (MQ=255)
                                         tcgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTg                                                                                                           <  1:6486915/151‑1 (MQ=255)
                                             cACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCa                                                                                                       >  2:4107949/1‑151 (MQ=255)
                                             cACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCa                                                                                                       >  1:8780795/1‑151 (MQ=255)
                                               ccGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAgg                                                                                                     <  1:3928798/151‑1 (MQ=255)
                                               ccGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAgg                                                                                                     <  1:1628622/151‑1 (MQ=255)
                                                 gTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGcc                                                                                                   >  2:10402588/1‑151 (MQ=255)
                                                 gTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGGTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGcc                                                                                                   <  2:8552151/151‑1 (MQ=255)
                                                   gCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCgg                                                                                                 <  2:6884767/151‑1 (MQ=255)
                                                   gCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCgg                                                                                                 <  2:1515267/151‑1 (MQ=255)
                                                     tCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTg                                                                                               >  1:7644636/1‑151 (MQ=255)
                                                       aCCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggc                                                                                             <  1:2047849/151‑1 (MQ=255)
                                                       aCCCAGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggc                                                                                             <  1:8431065/151‑1 (MQ=255)
                                                        cccTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggcg                                                                                            <  2:6104559/151‑1 (MQ=255)
                                                         ccTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTtgcgg                                                                                           >  1:5011303/1‑151 (MQ=255)
                                                         ccTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggcgg                                                                                           <  2:10191158/151‑1 (MQ=255)
                                                         ccTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggcgg                                                                                           <  1:8121792/151‑1 (MQ=255)
                                                          ctgctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggcggc                                                                                          >  1:8985972/1‑151 (MQ=255)
                                                           tgctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgcc                                                                                         >  2:5604348/1‑151 (MQ=255)
                                                             ctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgc                                                                                       >  1:5266707/1‑151 (MQ=255)
                                                              tgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCGTCTTGGCCAGGCCGGTGGCGgccgcc                                                                                      >  1:696745/1‑151 (MQ=255)
                                                                ttCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgccgc                                                                                    >  1:7816691/1‑151 (MQ=255)
                                                                  ccTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCGGACGGGAGTAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGccgccgccg                                                                                  <  2:1650962/151‑1 (MQ=255)
                                                                    tGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCggg                                                                                <  1:4155644/151‑1 (MQ=255)
                                                                        ggCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCt                                                                            <  2:4943611/151‑1 (MQ=255)
                                                                             tctATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCgg                                                                       >  2:212567/1‑151 (MQ=255)
                                                                               tATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGAt                                                                     >  1:4244709/1‑151 (MQ=255)
                                                                               aaTGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGAt                                                                     <  1:10673830/150‑1 (MQ=255)
                                                                                aTGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATg                                                                    >  2:7611258/1‑151 (MQ=255)
                                                                                  gccgccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGct                                                                  >  1:1342071/1‑151 (MQ=255)
                                                                                   ccgccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGctc                                                                 >  1:9099802/1‑151 (MQ=255)
                                                                                   ccgccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGctc                                                                 <  1:6698245/151‑1 (MQ=255)
                                                                                    cgccTGGTTCCGTGTGAAGAAGGACGCGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGTCCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGctct                                                                <  2:10629071/151‑1 (MQ=255)
                                                                                    cgccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGctct                                                                >  1:9815508/1‑151 (MQ=255)
                                                                                     gccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtctt                                                               >  1:3690827/1‑151 (MQ=255)
                                                                                     gccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtctt                                                               <  1:10451442/151‑1 (MQ=255)
                                                                                      ccTGGTTCCGTGTGATGAAGGACGAGGCGCGGGCCTGACGGGAGTAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtcttc                                                              <  2:3259869/151‑1 (MQ=255)
                                                                                       cTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtcttct                                                             <  2:2492539/151‑1 (MQ=255)
                                                                                       cTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtcttct                                                             >  1:4867293/1‑151 (MQ=255)
                                                                                        tGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTtctc                                                            <  2:3426548/151‑1 (MQ=255)
                                                                                          gTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCt                                                          <  1:2318656/151‑1 (MQ=255)
                                                                                          gTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCt                                                          <  1:4677189/151‑1 (MQ=255)
                                                                                           ttCCGGGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTg                                                         <  1:8327889/151‑1 (MQ=255)
                                                                                             ccGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCt                                                       >  2:907820/1‑151 (MQ=255)
                                                                                             ccGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCt                                                       >  1:539672/1‑151 (MQ=255)
                                                                                             ccGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCt                                                       >  2:8823425/1‑151 (MQ=255)
                                                                                              cGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCtt                                                      <  1:5662331/151‑1 (MQ=255)
                                                                                              cGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCtt                                                      <  1:266909/151‑1 (MQ=255)
                                                                                               gtgtGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTg                                                     <  2:4244756/151‑1 (MQ=255)
                                                                                                 gtgAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGc                                                   >  2:8195856/1‑151 (MQ=255)
                                                                                                       aagGACGAGGCGCGGGCCTGACGGGAGACGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGatcat                                             <  1:7264368/151‑1 (MQ=255)
                                                                                                        agGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGatcatc                                            >  2:5379405/1‑151 (MQ=255)
                                                                                                         ggACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCGTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCAtct                                           >  2:4609431/1‑151 (MQ=255)
                                                                                                           aCGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCg                                         >  1:2860822/1‑151 (MQ=255)
                                                                                                             gAGGCGCGGGCCTGACGGGAGAAGGGGTCGGGGCGATCGCG‑‑CCCCGACCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGaa                                       <  2:2541809/151‑1 (MQ=255)
                                                                                                                gcgcGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAagag                                    <  2:6625199/151‑1 (MQ=255)
                                                                                                                  gcgGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGcc                                  >  1:3812325/1‑151 (MQ=255)
                                                                                                                  gcgGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGcc                                  <  1:6173306/151‑1 (MQ=255)
                                                                                                                  gcgGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGcc                                  <  2:7023268/151‑1 (MQ=255)
                                                                                                                    gggCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCg                                >  2:137138/1‑151 (MQ=255)
                                                                                                                     ggCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGt                               >  2:2872337/1‑151 (MQ=255)
                                                                                                                       ccTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCg                             <  2:926866/151‑1 (MQ=255)
                                                                                                                           aCGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAggg                         <  1:10182058/151‑1 (MQ=255)
                                                                                                                             gggAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGcc                       <  1:10106129/151‑1 (MQ=255)
                                                                                                                                      ggACGGGGCGATCGCG‑‑CCCCGTCCGGCGACTCACCTGGCCGCGCCGCAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTg              <  2:8311793/151‑1 (MQ=255)
                                                                                                                                      ggACGGGGCG‑‑CGCGCGCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTGGGCGATCATCTCGAAGAGCCCGTCGAGGGACTGTTCGGTg              <  1:3368812/151‑1 (MQ=255)
                                                                                                                                           gggCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATa         <  2:95903/151‑1 (MQ=255)
                                                                                                                                            ggCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAg        >  2:7257734/1‑151 (MQ=255)
                                                                                                                                            ggCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAg        <  1:2886639/151‑1 (MQ=255)
                                                                                                                                                cgatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGcc      >  2:849731/5‑151 (MQ=255)
                                                                                                                                                 gatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGccc     >  2:5857301/4‑151 (MQ=255)
                                                                                                                                                 gatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGccc     <  2:10677845/148‑1 (MQ=255)
                                                                                                                                                 gatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGccc     <  1:1213691/148‑1 (MQ=255)
                                                                                                                                                  atcgcgCCCCGTCCGTCGTCTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGGGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCt    <  2:9746383/149‑1 (MQ=255)
                                                                                                                                                      ||                                                                                                                                                   
CTTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGCCCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTCG  >  NC_008463/1441707‑1442005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑