Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,354,163 (G)5→4 intergenic (‑48/+3) PA14_RS10945 ← / ← lipA lipase secretion chaperone/triacylglycerol lipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,354,1590G.99.7% 1726.5 / ‑2.1 350intergenic (‑44/+7)PA14_RS10945/lipAlipase secretion chaperone/triacylglycerol lipase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (170/179);  total (170/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 369 total aligned reads displayed.

GCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCATGC  >  NC_008463/2354009‑2354305
                                                                                                                                                      |                                                                                                                                                  
  gcTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggg                                                                                                                                                  <  1:9929114/151‑1 (MQ=255)
     ggCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggggtc                                                                                                                                               >  2:2033189/1‑149 (MQ=255)
      gCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggggtcc                                                                                                                                              >  1:9126712/1‑148 (MQ=255)
        cGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTa                                                                                                                                           >  1:43293/1‑151 (MQ=255)
          ggggCGCCGTCGCGGGTGCGGGGGAAGGTACCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAAACAGCAGGAGGATTTACTTCACGCGAGGGGGCTTCCGGTAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACa                                                                                                                                         <  1:6405844/151‑1 (MQ=255)
              cgcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCt                                                                                                                                     <  2:5815011/151‑1 (MQ=255)
               gcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTCCAGGCTg                                                                                                                                    >  2:5100356/1‑151 (MQ=255)
               gcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTg                                                                                                                                    >  1:5363859/1‑151 (MQ=255)
               gcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTg                                                                                                                                    <  2:2462090/151‑1 (MQ=255)
                ccGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTgg                                                                                                                                   >  1:4077359/1‑151 (MQ=255)
                 cGTCTCGGGTGCGGGGGAAGGTTCCAGCCCGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGc                                                                                                                                  >  1:264499/1‑151 (MQ=255)
                 cGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGc                                                                                                                                  >  2:1832610/1‑151 (MQ=255)
                  gTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCg                                                                                                                                 >  1:2355435/1‑151 (MQ=255)
                    ctcGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGtt                                                                                                                               <  1:5945900/151‑1 (MQ=255)
                      cGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCCGTGGAATGAGCAGGAGGATTTTCTTCACGCGTGGGGGCTTCCGGGAAAGGGCGGGGGCCGAGGCCCCGGCC‑GGGGTCCTACCGGCTGGCGtgct                                                                                                                             >  2:5673018/1‑151 (MQ=255)
                      cGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttct                                                                                                                             <  2:8229419/151‑1 (MQ=255)
                       gggCGCGCGCGAACGGTCCCGCCAGACGAACCAGGCCAGGCTGGCGGCGCCCGCCAGAGGACACCGCAGGCCGACGTTCGCCACGCGAGGGGGCTTCCGGGAAAGGGCCGGCGCCGCGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttctt                                                                                                                            <  2:8361724/151‑1 (MQ=255)
                        ggTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttcttc                                                                                                                           >  1:10006591/1‑151 (MQ=255)
                         gTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCa                                                                                                                          >  2:9746656/1‑151 (MQ=255)
                          tGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGACAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAg                                                                                                                         >  1:3835671/1‑151 (MQ=255)
                            cGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGc                                                                                                                       <  2:3412174/151‑1 (MQ=255)
                            cGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGc                                                                                                                       >  2:6841283/1‑151 (MQ=255)
                                ggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGtt                                                                                                                   >  2:10207722/1‑151 (MQ=255)
                                ggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGtt                                                                                                                   <  2:10030095/151‑1 (MQ=255)
                                ggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGtt                                                                                                                   >  1:8104472/1‑151 (MQ=255)
                                 gAAGGTTCCCGCCAGACGCCCCCGGCCAGGCTGGCGGCGCACGCCAGGGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCGTCCGGGAAAGGGCCGGGGCCGCGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTg                                                                                                                  <  2:8658744/151‑1 (MQ=255)
                                 gAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTg                                                                                                                  >  2:4553720/1‑151 (MQ=255)
                                   aGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGc                                                                                                                <  2:10500657/151‑1 (MQ=255)
                                    tgTTCCAGCCAGACGAACGTGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGTTGATTTTCTTCACGCGTGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGTCCCCGGCC‑GGGGTCCTAAAGGCTGGCGTTCTTCAGGCGGTTGGCg                                                                                                               <  2:2763468/150‑1 (MQ=255)
                                    ggTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCg                                                                                                               >  1:5003032/1‑151 (MQ=255)
                                      ttCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTg                                                                                                             <  1:2260174/151‑1 (MQ=255)
                                      ttCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTg                                                                                                             <  1:10271732/151‑1 (MQ=255)
                                       tCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGc                                                                                                            >  1:2930924/1‑151 (MQ=255)
                                          agccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTgg                                                                                                         >  1:9683199/1‑151 (MQ=255)
                                           gccagACGAACCAGGCCAGGCTGGCGGCGACCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGCGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGc                                                                                                        <  1:7217269/151‑1 (MQ=255)
                                           gccagACGAACCAGGCCAGGCTGGCGGCGAACGCCCGTGTAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGc                                                                                                        <  1:4458925/151‑1 (MQ=255)
                                           gccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGc                                                                                                        <  1:8732840/151‑1 (MQ=255)
                                            ccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCg                                                                                                       >  1:1096712/1‑151 (MQ=255)
                                              agACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGt                                                                                                     <  1:6757042/151‑1 (MQ=255)
                                               gACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTa                                                                                                    <  1:8905865/151‑1 (MQ=255)
                                                  gAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGAc                                                                                                 >  2:1301189/1‑151 (MQ=255)
                                                  gAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGAc                                                                                                 <  2:7835418/151‑1 (MQ=255)
                                                   aaCCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACg                                                                                                >  1:475092/1‑151 (MQ=255)
                                                    aCCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTACTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGc                                                                                               <  1:6301242/151‑1 (MQ=255)
                                                     ccaggccaggCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCCCGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCt                                                                                              <  2:9876378/151‑1 (MQ=255)
                                                     ccaggccaggCGGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCt                                                                                              <  1:1961001/151‑1 (MQ=255)
                                                       aggccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGa                                                                                            >  1:6538905/1‑151 (MQ=255)
                                                         gccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATGTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGAcc                                                                                          <  2:3654149/151‑1 (MQ=255)
                                                           caggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCgg                                                                                        >  1:6036585/1‑151 (MQ=255)
                                                           caggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCgg                                                                                        >  2:8803790/1‑151 (MQ=255)
                                                            aggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCggg                                                                                       >  1:7497936/1‑151 (MQ=255)
                                                                  gcggcgAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGCGGGGGCTTCAGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtc                                                                                 <  1:848679/151‑1 (MQ=255)
                                                                    ggcgAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtctc                                                                               <  1:9305051/151‑1 (MQ=255)
                                                                             cAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCGGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCt                                                                      >  1:4553793/1‑151 (MQ=255)
                                                                                tGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGt                                                                   <  2:3792547/151‑1 (MQ=255)
                                                                                  gAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGa                                                                 <  2:1482947/151‑1 (MQ=255)
                                                                                   aaTCAGCAGGAGGTTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAg                                                                <  2:3548663/151‑1 (MQ=255)
                                                                                    aTCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAgg                                                               <  1:1579468/151‑1 (MQ=255)
                                                                                       agcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCg                                                            >  2:2167278/1‑151 (MQ=255)
                                                                                         cagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGaa                                                          >  1:296085/1‑151 (MQ=255)
                                                                                             aggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGAcc                                                      >  2:446948/1‑151 (MQ=255)
                                                                                              ggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCt                                                     >  2:7136379/1‑151 (MQ=255)
                                                                                              ggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCt                                                     >  1:8040748/1‑151 (MQ=255)
                                                                                              ggaTTTTCGTCACGCGAGGGGGCTTCCGGGACAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGACGACCt                                                     <  2:5241282/151‑1 (MQ=255)
                                                                                                     cttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCAc                                              >  1:8315582/1‑151 (MQ=255)
                                                                                                     cttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGATGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCAc                                              >  1:1278343/1‑151 (MQ=255)
                                                                                                      ttcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCAcc                                             <  2:3172522/151‑1 (MQ=255)
                                                                                                        cACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTc                                           <  2:7261911/151‑1 (MQ=255)
                                                                                                          cgcgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGt                                         <  1:9434934/151‑1 (MQ=255)
                                                                                                            cgcgggggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTcc                                       <  1:4934454/148‑1 (MQ=255)
                                                                                                            cgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTcc                                       >  1:2094663/1‑151 (MQ=255)
                                                                                                             gAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCa                                      >  1:5413469/1‑151 (MQ=255)
                                                                                                             gAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCa                                      <  1:3755485/151‑1 (MQ=255)
                                                                                                                  ggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggtgg                                 <  1:7803728/151‑1 (MQ=255)
                                                                                                                   gCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggtggt                                <  2:4982809/151‑1 (MQ=255)
                                                                                                                   gCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggtggt                                <  2:7442382/151‑1 (MQ=255)
                                                                                                                    cTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGtt                               >  1:3126917/1‑151 (MQ=255)
                                                                                                                       ccGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCAt                            >  2:7637768/1‑151 (MQ=255)
                                                                                                                         gggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATcc                          >  1:1819006/1‑151 (MQ=255)
                                                                                                                         gggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATcc                          >  2:3323952/1‑151 (MQ=255)
                                                                                                                            aaaGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCggg                       >  2:10094704/1‑150 (MQ=255)
                                                                                                                            aaaGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGt                       >  2:9446404/1‑151 (MQ=255)
                                                                                                                            aaaGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGt                       >  2:9362500/1‑151 (MQ=255)
                                                                                                                              aGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAg                     <  2:4392714/151‑1 (MQ=255)
                                                                                                                               gggccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGt                    <  1:5223050/151‑1 (MQ=255)
                                                                                                                                ggccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGtt                   <  2:4722506/151‑1 (MQ=255)
                                                                                                                                ggccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGtt                   <  2:7895255/151‑1 (MQ=255)
                                                                                                                                ggccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGtt                   <  1:7943624/151‑1 (MQ=255)
                                                                                                                                   cggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTc                >  2:5917285/1‑151 (MQ=255)
                                                                                                                                   cggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTc                >  1:3307584/1‑151 (MQ=255)
                                                                                                                                   cggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTc                <  2:1221065/151‑1 (MQ=255)
                                                                                                                                   cggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTc                <  1:6985768/151‑1 (MQ=255)
                                                                                                                                     gggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTcgc              <  2:10672823/151‑1 (MQ=255)
                                                                                                                                      ggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTcgcg             >  1:5616737/1‑151 (MQ=255)
                                                                                                                                         cgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAt          >  1:10321505/1‑151 (MQ=255)
                                                                                                                                          gaggCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGTAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATc         >  1:5841462/4‑151 (MQ=255)
                                                                                                                                             gCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCAcc      >  2:6897108/1‑151 (MQ=255)
                                                                                                                                             gCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCAcc      >  1:6265283/1‑151 (MQ=255)
                                                                                                                                               cccGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCAt    >  2:6088981/1‑151 (MQ=255)
                                                                                                                                                ccggcc‑ggGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCATg   <  2:6288990/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGATCACCATGC  >  NC_008463/2354009‑2354305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑