Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,954,0030TG26.2% 855.8 / 44.9 375Q75P (CAG→CCG) PA14_RS13685DUF6162 family protein
Reads supporting (aligned to +/- strand):  ref base T (86/185);  new base G (94/2);  total (188/187)
Fisher's exact test for biased strand distribution p-value = 7.17e-34
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 378 total aligned reads displayed.

CCCAGGTAGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGCCAGG  >  NC_008463/2953853‑2954152
                                                                                                                                                      |                                                                                                                                                     
cccAGGTAGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCt                                                                                                                                                       <  2:10570564/151‑1 (MQ=255)
       aGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTc                                                                                                                                                <  1:4076416/151‑1 (MQ=255)
        gCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCGGGCGGGCCGGGATGTcg                                                                                                                                               >  1:3430559/1‑150 (MQ=255)
        gCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCt                                                                                                                                               <  1:3382144/151‑1 (MQ=255)
         cTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGTAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGGGCGGCGGGGTGTcgt                                                                                                                                              >  1:7205200/1‑149 (MQ=255)
         cTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCtt                                                                                                                                              <  1:2569101/151‑1 (MQ=255)
           cGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTcg                                                                                                                                            >  1:2332283/1‑151 (MQ=255)
            ggACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTcgg                                                                                                                                           <  1:6374521/151‑1 (MQ=255)
             gACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTcggc                                                                                                                                          <  2:2623775/151‑1 (MQ=255)
              aCGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTcggcg                                                                                                                                         <  2:1552181/151‑1 (MQ=255)
                gCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCggcggc                                                                                                                                       <  1:8668684/151‑1 (MQ=255)
                    ggcggTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGACGCCCTCGGCGGCCCGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGGGCGCCGGGCTGTGGTcggcggggccc                                                                                                                                   >  1:10565836/1‑146 (MQ=255)
                    ggcggTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGAcc                                                                                                                                   <  1:5432174/151‑1 (MQ=255)
                     gcggTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCt                                                                                                                                  <  1:975555/151‑1 (MQ=255)
                      cggTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCCCCGGGATGGCTTCGGCGGCGAccag                                                                                                                                 >  2:8869174/1‑149 (MQ=255)
                           tCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGt                                                                                                                            <  2:5503464/151‑1 (MQ=255)
                            cGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTGCCGGCGCGCCGGGGTGTCTTCGGCGGCGCCCAGcccgtc                                                                                                                           >  2:915908/1‑146 (MQ=255)
                             gCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGGTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCGGGGTGGCTTCGGCGGCGCCCGGGAGGTCg                                                                                                                          >  1:10221107/1‑151 (MQ=255)
                              ccGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCgg                                                                                                                         <  1:9887931/151‑1 (MQ=255)
                               cGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGc                                                                                                                        <  1:1738476/151‑1 (MQ=255)
                                    cTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCCGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGAGGTCGTCGGCGGCGACCTGCAGGTCGGCATAGa                                                                                                                   >  2:774277/1‑151 (MQ=255)
                                    cTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCGGGGGCGGCGGGATGGGGTGGGGGGCGACCAGCACGTGGGcggaga                                                                                                                   >  1:2977974/1‑146 (MQ=255)
                                     tCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGCTGTCTTGGGCGGCGGCCAGCAGGTCgggctatat                                                                                                                  >  2:1043639/1‑144 (MQ=255)
                                      ccAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGGTGTCGTCGGGGGCGTCCTGCAGGTCGGGATAGATg                                                                                                                 >  2:6266123/1‑151 (MQ=255)
                                        aGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGGCTTCGGCGGCGGCCAGCAGGTCGGCATAGAAGcc                                                                                                               >  1:6633727/1‑151 (MQ=255)
                                         gCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGccc                                                                                                              >  2:9730690/1‑151 (MQ=255)
                                          cGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCTGGCTGTCTTCGGCGGCGACCTGCAGGTCGGCAGAGATGcccg                                                                                                             >  2:10125061/1‑150 (MQ=255)
                                          cGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGACCTGCCGGTCGGCATAGATGcccg                                                                                                             >  2:6928262/1‑150 (MQ=255)
                                            gTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGt                                                                                                           <  1:4290282/151‑1 (MQ=255)
                                             tGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGtt                                                                                                          <  2:4299505/151‑1 (MQ=255)
                                              gCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTc                                                                                                         <  1:7315875/151‑1 (MQ=255)
                                                tCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGGCGGGGGGTGTTCGGCGGCGGCCTGCGGGGCGGGCTAGATGCCCTGGTcgg                                                                                                       >  1:1266328/1‑151 (MQ=255)
                                                 cgccgcGGCGGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCACGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTcggc                                                                                                      <  1:7587854/151‑1 (MQ=255)
                                                  cccgcGGCCGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCCGCGGCCAGGTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGCCGACCTGCAGGACGGCAAAGATGCCCAGTTcggcg                                                                                                     <  2:9857371/150‑1 (MQ=255)
                                                   ccgcGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCAGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTcggcgg                                                                                                    >  2:4154366/1‑151 (MQ=255)
                                                   ccgcGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGGTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCAGGCTGTGGTCGGCGGCGCCCGGCAGGTCGGCCAAGATGCCCAGTGcggcgg                                                                                                    >  2:10345511/1‑151 (MQ=255)
                                                    cgcgGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCTGGATGTGTTGGGGGGCGGCCTGGCGGTCGGCAGTGTTGCCCTGTTGggcggc                                                                                                   >  1:10000342/1‑151 (MQ=255)
                                                    cgcgGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGACCGGCCGGTCGGCAGAGATGCCCGGTTCggcggc                                                                                                   >  1:6833948/1‑151 (MQ=255)
                                                      cgGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCAt                                                                                                 <  1:7052190/151‑1 (MQ=255)
                                                        gctgctCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGGTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCGGGATGTCTTCGGCGGCGGCCGGCAGGTCGGCATAGATGCCCGGTGGggcggcggtc                                                                                               >  1:3752977/1‑147 (MQ=255)
                                                            ctCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGCTGTCTTCGGCGGCGGCCGGGCGGTCGGGAGGCTTTGCCTGGTCGGCGGCCTGCAGGt                                                                                           >  1:4885452/1‑151 (MQ=255)
                                                             tCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTc                                                                                          <  2:3905918/151‑1 (MQ=255)
                                                                cggcgACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTGCCGGCGCGCCGGGGTGTCTTCGGCGGCGGCCAGCAGGTGGGCAGAGATGACCGGGGGGGCGGCCGTCAgggggag                                                                                       >  1:4616457/1‑146 (MQ=255)
                                                                cggcgACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAg                                                                                       <  2:8668771/151‑1 (MQ=255)
                                                                cggcgACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTGCGGCGGCGACCTGCAGGTCGGCAAAGATGCCCTGGTCGGCGGCATTCAGGTCGAg                                                                                       >  2:5955103/1‑151 (MQ=255)
                                                                cggcgACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGGCCTGCCGGTCGGCATAGCTGCCCTGTTCGGCGGCCTTCAGGTCgcg                                                                                       >  2:6620146/1‑149 (MQ=255)
                                                                 ggcgACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCCTAGATGCCCTGTTCGGCGGCATTCAGGTCGAGc                                                                                      >  2:5147169/1‑151 (MQ=255)
                                                                   cgACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCg                                                                                    <  1:3886194/151‑1 (MQ=255)
                                                                    gACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCgg                                                                                   <  1:5992871/151‑1 (MQ=255)
                                                                    gACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGGTGTCGTCGGCGGCGTCCTGCGGGTCGGCATAGCTGCCCGGTTCGGCGGCAATAAGGTCGAGCCgg                                                                                   >  2:2464392/1‑151 (MQ=255)
                                                                      cGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGGTGTCGTCGGCGGCGTCCGGCAGGGCGGCATAGAAGCCCTGTTCGGCGGCCGTAAGGTGGAGGCCGGc                                                                                 >  1:2115117/1‑151 (MQ=255)
                                                                      cGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGGCGGCCTAGATGCCCTGGTCGGCGGCCATCAGGTCGAGCCGGGc                                                                                 >  2:1772179/1‑151 (MQ=255)
                                                                       gTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCg                                                                                >  2:10684283/1‑151 (MQ=255)
                                                                        tCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTGTTAGGCGGCGGCCAGCAGGTCGGCAGAGATGACCAGGTGGGCGGCCTACATGTCGATCAGGGCgg                                                                               >  1:114840/1‑150 (MQ=255)
                                                                             gtcgcaGGGCGGGAAGCCCTCGGCGGCGAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCAcc                                                                          <  2:9245093/146‑1 (MQ=255)
                                                                              acgaAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCt                                                                         <  2:8141516/151‑1 (MQ=255)
                                                                               cgaCGGGCTCGAAGCCCACGGCGGCCTGGTCGTAGCCGGAGGGCGCGGCGTCGCCGTCTTCCAGGCGGGCCTTGATGTCTGCGTCTTCGCCCTGCAGGTCGGCATAGATGGCCTGTTCGGGGGCATTCTGGTCGAGCCGGGCGTCCACCtg                                                                        <  2:4480791/151‑1 (MQ=255)
                                                                               cgaAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGTCCTGCAGGTCGGCATAGATGCCCAGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCAccag                                                                        >  2:4264658/1‑149 (MQ=255)
                                                                               cgaAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCtg                                                                        <  1:9254315/151‑1 (MQ=255)
                                                                                gaAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTGTTCGGCGGCGCCCTGCAGGGCGGCCGAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCagg                                                                       >  2:9335836/1‑151 (MQ=255)
                                                                                  aGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGGTGTCTTCGGCGGCGACCTGCTGGTCGGCCTAGATGACCTGTTCGGCGGCCGTCAGGTCGAGCCGGGCGTCCCCCagggg                                                                     >  1:882541/1‑149 (MQ=255)
                                                                                     gCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGc                                                                  <  1:6269843/151‑1 (MQ=255)
                                                                                      cGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCg                                                                 <  2:7552059/151‑1 (MQ=255)
                                                                                      cGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGTTGTCTGCGGCGGCGGCCAGCCGGGCGGGCTTGATGCCCGGTTAGGCGGCCGTCAGGTCGAGCCGGGCGTCCACCAGGTGGGCg                                                                 >  1:9534677/1‑151 (MQ=255)
                                                                                       gggAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGt                                                                <  1:2862897/151‑1 (MQ=255)
                                                                                        ggAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTc                                                               <  1:7973784/151‑1 (MQ=255)
                                                                                        ggAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCAGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCgcc                                                               <  1:9862358/151‑3 (MQ=255)
                                                                                        ggAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGCCCGGCAGGTCGGCCTAGATTCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGGCCCCCAGGTGGGCGTc                                                               >  1:10682545/1‑151 (MQ=255)
                                                                                        ggAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGACCAGCAGGTCGGCAGAGATTCCCTGTTAGGCGGCAGTCAGGTCGAGCCGGGCGTCCACCAgggggggggg                                                               >  1:5821407/1‑147 (MQ=255)
                                                                                         gacgCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGGCCAGGCCCGCGGGGATGTGGGGTGCGGCGAGCTGCTGGTGGGCGGATATGCCCGTTTCGGCGGCGTTCAGGGCGTGCCGGGGGTGCGCCAGGGGGGGGtgc                                                              >  2:2147441/4‑149 (MQ=255)
                                                                                         gAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCGGGATGTCTTCGGCGGCGCCCTGCAGGTCGGCAAAGATGCCCTGTTCGGCGGCAATAAGGTCGATCCGGGCGTCCACCAGGGGGGCGTcc                                                              >  2:4643048/1‑151 (MQ=255)
                                                                                          aaGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCGGGCTGTCTTCGGCGGCGACCTGCAGGTCGGCATAGCAGCCCTGTTCGGCGGCATTAAGGTCGAGCCGGGCGTCCACCTGGGGGGGGGCCa                                                             >  2:5384089/1‑151 (MQ=255)
                                                                                          aaGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGCTGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCa                                                             <  1:3572829/151‑1 (MQ=255)
                                                                                          aaGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTAGTCGGCGGCGACCAGCAGGTCGGCATAGATGACCCGGTCGGCGGCAAAAAGCTCGAGCCGGGCGTCCACCAGGTGGGCGGccc                                                             >  1:6673864/1‑150 (MQ=255)
                                                                                            gCCCACGGCGGCCAGTTCGACGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGAGGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCGGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAgg                                                           <  1:8308126/151‑1 (MQ=255)
                                                                                             cccTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCGTCGGCGGGGACCTGCCGGTCGGCATAGAAGCCCTGTTCGGCGGCATTAAGGTCGTGCCGGGCGTACACCAGGGGGGCGGCCAggg                                                          >  2:4915452/1‑151 (MQ=255)
                                                                                                   gcggcCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCCCCGGGATGTCTTCGGCGGCGACCAGCCGGGCGCCAAAGAAGCCCACGGCGGCGGCATAAAGGTCGAGCCGGGCGTCCCCCAGGTGGGCGGCCCGCGCTTgcg                                                    >  2:1835419/1‑151 (MQ=255)
                                                                                                   gcggcCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTgcg                                                    <  1:8453860/151‑1 (MQ=255)
                                                                                                      gcCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGACCAGCAGGTCGGCATAGATGCCCTGTTCGGCGGCAATCAGGGCGAGCCGGGCGTCCACCAGGTGGGCGTCCAGGGCTTgcgcgt                                                 >  2:5679823/1‑149 (MQ=255)
                                                                                                       ccAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTc                                                <  2:5901714/151‑1 (MQ=255)
                                                                                                          gTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCATGGATGTCTTCGGCGGCGAGCTGCAGGTCGGCATAGAGGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGAGTCCAGGGCTTGCGCTTCATg                                             <  1:895789/151‑1 (MQ=255)
                                                                                                           ttCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCTGGATGTGTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCGGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCAGGTGGGCGGCCAGGGCGTGCGCTTCATgg                                            >  2:2056414/1‑151 (MQ=255)
                                                                                                                  aCGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGActgct                                     <  1:904978/151‑1 (MQ=255)
                                                                                                                  aCGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTAAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGActgct                                     >  2:4809045/1‑151 (MQ=255)
                                                                                                                      aGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGgcg                                 <  2:7369097/151‑1 (MQ=255)
                                                                                                                       gggCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCAGCATGTAGGCATAGATGCCCGGTTCGGCGGCATTAAGGTCGAGCCGGGCGGACACCAGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGgcgc                                >  2:1203212/1‑151 (MQ=255)
                                                                                                                        ggCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCa                               <  1:10160628/151‑1 (MQ=255)
                                                                                                                            cgGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGccc                           <  2:357751/151‑1 (MQ=255)
                                                                                                                               cTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGac                        <  1:1995963/151‑1 (MQ=255)
                                                                                                                                gtCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCGTGCGCTTCATGGGACTGCTGGCGCAGCCCGacc                       <  1:7447085/150‑1 (MQ=255)
                                                                                                                                  cGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCAGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGaccac                     >  2:7449288/1‑151 (MQ=255)
                                                                                                                                      gtcgtcCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTg                 <  1:2787652/151‑1 (MQ=255)
                                                                                                                                           cccgccgcgcCTGCATCACTGCGGCGCCGACCCGCCGGTCGGCACAGATGCCCGGTTCGCCGGCATACAGGACGAGCCGGGCGTCCCCCTGGTGGGCGACCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGGggcggc            <  2:1943627/146‑1 (MQ=255)
                                                                                                                                              ggCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAg         >  1:1493503/1‑151 (MQ=255)
                                                                                                                                               gcgcgcCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCAGTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCATGGCTAGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAgg        <  2:7160092/151‑1 (MQ=255)
                                                                                                                                                 gcgcCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGc      <  2:3400274/151‑1 (MQ=255)
                                                                                                                                                 gcgcCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGc      >  1:3090383/1‑151 (MQ=255)
                                                                                                                                                  cgcCTGGAGGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGcc     <  2:8076321/151‑1 (MQ=255)
                                                                                                                                                   gcCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGCCa    <  2:8416478/151‑1 (MQ=255)
                                                                                                                                                   gcCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGCCa    >  2:9608313/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CCCAGGTAGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGCCAGG  >  NC_008463/2953853‑2954152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑