Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,347,5800TG28.3% 704.0 / 28.7 348intergenic (‑79/+150)PA14_RS19855/PA14_RS19860hypothetical protein/zonular occludens toxin family protein
Reads supporting (aligned to +/- strand):  ref base T (80/138);  new base G (85/1);  total (208/140)
Fisher's exact test for biased strand distribution p-value = 2.83e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 356 total aligned reads displayed.

GGGGGAGTCCAGCAGTTGTCCGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAATTCCCAATCGAT  >  NC_008463/4347430‑4347730
                                                                                                                                                      |                                                                                                                                                       
gggggAGTCCAGCAGTTGTCCGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCt                                                                                                                                                         <  1:1646702/151‑1 (MQ=255)
    gAGTCCAGCAGTTGTCCGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCATCgg                                                                                                                                                     >  2:3673625/1‑151 (MQ=255)
      gTCCAGCAGTTGTCCGTCGGGTTGCAACAGGAGGCGGTGTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGGTTATCTGACGGGTTACAGGGGCGTCGGCCGCGCCTTCGGcc                                                                                                                                                   >  2:3403206/1‑151 (MQ=255)
       tCCAGCAGTTGTCCGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCt                                                                                                                                                  <  2:1111741/151‑1 (MQ=255)
              gTTGTCCGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGcgc                                                                                                                                           >  1:7002309/1‑149 (MQ=255)
              gTTGTCCGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCGATCGCTc                                                                                                                                           >  2:8622068/1‑151 (MQ=255)
                 gTCCGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCATCGGCCGATCGCGC‑ATg                                                                                                                                       >  2:9377140/1‑151 (MQ=255)
                  tCCGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGCCTAACGCGC‑ATGc                                                                                                                                      >  1:10318342/1‑151 (MQ=255)
                   ccGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGCCTATCGCTC‑ATGcc                                                                                                                                     >  2:8411343/1‑151 (MQ=255)
                   ccGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCATCGGGCTATCGCTC‑AAGcc                                                                                                                                     >  1:438749/1‑151 (MQ=255)
                     gTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCGGCGGCCGATCGCGC‑AAGCCtg                                                                                                                                   >  1:3799732/1‑150 (MQ=38)
                     gTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCtt                                                                                                                                   <  2:4259378/151‑1 (MQ=255)
                       cGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTGGGCCGATCGCTC‑AGGCCGTgc                                                                                                                                 >  1:2543028/1‑151 (MQ=255)
                          gTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCGTCGGCCGATAGCAC‑AATCCtttgggt                                                                                                                              >  1:746682/1‑146 (MQ=38)
                          gTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCCCCATCGGCCAATCGCTC‑ATTCCATTCGCt                                                                                                                              >  1:6755143/1‑151 (MQ=38)
                           ttGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTc                                                                                                                             <  2:2984364/151‑1 (MQ=255)
                            tGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTcc                                                                                                                            <  1:4165712/151‑1 (MQ=255)
                             gCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTccc                                                                                                                           <  1:3384950/151‑1 (MQ=255)
                              cAACTGGAGGCGGATTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAACCACAGGGAGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCccg                                                                                                                          <  2:2053941/151‑1 (MQ=255)
                              cAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCCCCAAACGCCGATCGCAC‑AATCCATTCGCTcccc                                                                                                                          >  1:2921430/1‑150 (MQ=255)
                               aaCAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTAGGCCTATAGCTC‑ATTCCTTGCGCTacccg                                                                                                                         >  1:8789205/1‑149 (MQ=255)
                                aCAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCccggc                                                                                                                        <  2:9131925/151‑1 (MQ=255)
                                   ggaggCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCccggccgg                                                                                                                     <  1:8731251/151‑1 (MQ=255)
                                   ggaggCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCATGCGCTCccggccgg                                                                                                                     >  2:8727068/1‑151 (MQ=255)
                                     aggCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCcggcg                                                                                                                   <  2:4560554/151‑1 (MQ=255)
                                     aggCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCcggcg                                                                                                                   <  1:7614559/151‑1 (MQ=255)
                                       ggggTTTTTTTGATTCGGTTTCACTTTCTTCTTGGGCTTACCCTGCAATGTCCATTATTGTCCAAAACACAGGGCTTTTATCTGACGTGTTACTGGGGCGTCGGCGGCGCCTTGGGCCTATCGATC‑TTGCATTGCGCTCCCGGCCggcggc                                                                                                                 <  2:1870273/149‑1 (MQ=255)
                                       gCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCggcggc                                                                                                                 <  2:6800364/151‑1 (MQ=255)
                                        cGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGgcggcg                                                                                                                <  1:4616185/151‑1 (MQ=255)
                                         ggTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCGTCGGCCGATAGCTC‑ATGGCTTGCGCTCCCGGCCGgcggcgc                                                                                                               >  1:7706028/1‑151 (MQ=255)
                                         ggTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCGTCGGCCAAAAGCAC‑ATTCCGTGCGCGCCCGGCCCgcggcgc                                                                                                               >  1:3136394/1‑151 (MQ=37)
                                          gTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGgcggcgcg                                                                                                              <  1:141194/151‑1 (MQ=255)
                                              tgatgaATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGgccc                                                                                                          <  2:5182608/151‑1 (MQ=255)
                                                atgaATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGGCTTCGGCCGATAGCTC‑ATGCCGTGCGCTCCCGGCCCGCGGCGCGgccccc                                                                                                        >  1:9544242/1‑149 (MQ=37)
                                                   aaTCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATTCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGcccc                                                                                                     >  2:7468290/1‑151 (MQ=255)
                                                     tCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCATAGGGCGATCGCGC‑ATTCCGTGCGCTCCCGGCCGGCGGGGCGGCCCGccccac                                                                                                   >  1:3892825/1‑149 (MQ=37)
                                                      cGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGGGCCGTAGGCCTATAGCTC‑AAGCCTGGCGCGACCCGCCGGCGGCGCCGCCCGCCCcaca                                                                                                  >  1:3576086/1‑151 (MQ=25)
                                                       ggTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCATAGGCCGATACCACAATGCCTT‑CGCGCCCCGCCCGGGGCGCGGCCCCCCCCCCaa                                                                                                 >  1:49486/1‑150 (MQ=25)
                                                       ggTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCAt                                                                                                 <  1:6943539/151‑1 (MQ=255)
                                                       ggTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGCCTATCGCAC‑ATGCCTTGCGCACCCGGCCGGCGGCGCGGCCCGCCCCACAt                                                                                                 >  2:4972352/1‑151 (MQ=255)
                                                          ttCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTAGGCCTATAGCTC‑AAGCCATTCGCTCCCCGCCGGCGGCGCGGCCCCCCCcacaatgc                                                                                              >  1:9910792/1‑147 (MQ=25)
                                                           tCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCGGCGGCCGAACGCGC‑AAGCCGTGCGCGCCCCGCCCGCGGCGCGGCCCCCCCCCCATggcg                                                                                             >  1:10056851/1‑151 (MQ=25)
                                                           tCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTAAAGCAC‑AAGCCTTGGGCTCCCCGCCGGCGGCGCGGCCCCCCCCGCAAggcg                                                                                             >  1:9271239/1‑151 (MQ=25)
                                                             aCTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGCCTATCGCTC‑AAGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATggcggc                                                                                           >  2:10604837/1‑151 (MQ=255)
                                                              cTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCGTCGGCCAATAGCGC‑AAGCCATGCGCGCCCCGCCGGCGGCGCGGCCCCCCCCACAAggcgggg                                                                                          >  1:1831273/1‑149 (MQ=25)
                                                              cTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGgcggcg                                                                                          <  2:3841375/151‑1 (MQ=255)
                                                              cTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGCCAATCGCTC‑ATGCCATGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCCCATGgcggcg                                                                                          >  1:7684082/1‑151 (MQ=255)
                                                              cTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGGGTTACAGGGGCGTCGGCCGCGCCGTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCGCATGgcggcg                                                                                          >  2:9543976/1‑151 (MQ=255)
                                                                 tcttcATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCCCGCCGTAGGCCAATAGCTC‑ATTCCATTCGCTCCCCGCCCGCCGCGCGGCCCCCCCCACAAGGCGGCGccc                                                                                       >  1:3196441/1‑151 (MQ=25)
                                                                 tcttcATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGCCTATCGCTC‑ATGGCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCCTGGCGGCGccc                                                                                       >  2:710998/1‑151 (MQ=255)
                                                                 tcttcATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCACATGGCGGCGccc                                                                                       >  2:6638928/1‑151 (MQ=255)
                                                                  cttcATGGGCTGACCCAGCAATGTCCATTAATGTCCAAACCACCGGGCGTTTATCTGACGAGTTCCAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑AGGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGcccc                                                                                      <  2:6170635/151‑1 (MQ=25)
                                                                     cATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCGTCGGCCAATAGCTC‑AATACATGCGCGCCCCGCCGGCGGCGCGGGCCCCCCCACAAGGCGGGGCCCCAAc                                                                                   >  1:5451624/1‑151 (MQ=21)
                                                                      aTGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTAcc                                                                                  <  1:6844625/151‑1 (MQ=255)
                                                                      aTGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTAcc                                                                                  <  1:6068365/151‑1 (MQ=255)
                                                                           cTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCAATCGCTC‑ATGCCTTTCGCTCCCGGCCGGCGGCGCGGCCCGCCCCACAAGGCGGCGCCCCAACccccgc                                                                             >  2:596537/1‑151 (MQ=25)
                                                                            ttACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCt                                                                            <  1:6403325/151‑1 (MQ=255)
                                                                             tACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGCCTATCGCTC‑CTGCCTTGCGCGCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCAACCGCCGCTa                                                                           >  2:3216488/1‑151 (MQ=255)
                                                                              aCCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCCTCGGCCTAACGCTC‑AAGCCTAGCGCACCCCGCCGGCGGCGCCGCCCCCCCCACAATGCGGCGCCCCAACCGCCGCTAg                                                                          >  1:5873457/1‑151 (MQ=21)
                                                                               cccAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAgc                                                                         <  1:2815284/151‑1 (MQ=255)
                                                                                 cAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCGTCGGCCTAACGCGC‑AAGGCATGCGCGCCCCGCCCGCGGCGCGGCCCCCCCCACAAGGCGGCGCCCCAACCCCCCCGAgccc                                                                       >  1:7290061/1‑149 (MQ=21)
                                                                                    cAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGGCGCGCCGTAGGCCGAAAGCGC‑ATGCCGAGCGCGCCCCGCCCGCGGGGCGGGCCCCCCCGCCAGGCGGCGGCCCAACCCCCGCCAGCGcccg                                                                    >  1:5705884/1‑149 (MQ=16)
                                                                                     aaTGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCGATCGCTC‑ATGCCTTGGGCTCCCGGCCGGCGGCGCGGCCCGCCCCACCTGGCGGCGCCCCGAACGCCGCAAGCGCCGtc                                                                   >  2:4660979/1‑151 (MQ=25)
                                                                                     aaTGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGGCGATAGCGC‑AGGCCGTGCGCGCCCCGCCGGCGGCGCGGCCCCCCCCACATGGGGGCGCCCCGACCCCCCCGCGCGcccgc                                                                   >  1:8348518/1‑148 (MQ=21)
                                                                                      aTGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGtca                                                                  <  2:9098897/151‑1 (MQ=255)
                                                                                        gTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGtcatc                                                                <  2:8900749/151‑1 (MQ=255)
                                                                                         tCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGGCGAACGCTC‑CTGCCGGGGGCTCCCGGGCGGCGGGGCGGCCCGCCCCTCAATGCGGCGCCCCGCCCGCCGCGAGCGCCGgcctca                                                               >  2:9015626/1‑151 (MQ=21)
                                                                                         tCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCGAGCGCCGtcatcc                                                               >  2:2553202/1‑150 (MQ=255)
                                                                                         tCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCCCCGTAGGCCAATCGCGC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCACATGGGGGCGCCCCAACCGCCCCTAGCGCCGgcaaaa                                                               >  1:5991274/1‑148 (MQ=21)
                                                                                               aaTGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTcc                                                         <  2:4310687/151‑1 (MQ=255)
                                                                                                aTGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCGTCGGCCTATCGCGC‑ATGCCTTGCGCGCCCGGCCGGCGGCGCGGCCCGCCCCGCATGGCGGCGCCCCTACCGCCCCTAGCGCCGAACTAACCGTcca                                                        >  2:6601406/1‑151 (MQ=21)
                                                                                                  gTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGGCTATCGCTC‑ATGCCTTGCGCACCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCAACCGCCGCTAGCGCCGGCATCACCGTccccc                                                      >  2:10084980/1‑151 (MQ=255)
                                                                                                  gTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTccacc                                                      <  1:7925982/151‑1 (MQ=255)
                                                                                                  gTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCATCGGCCAATAGCTC‑ATGCCGTTCGCTCCCGGCCGGCGGCGCGGCCCGCCCCACAATGCGGCGCCCCCAACGCCGCTCTCGCCGTCATCACCCGccacc                                                      >  2:9888706/1‑151 (MQ=18)
                                                                                                   tCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTccacca                                                     <  2:2717591/151‑1 (MQ=255)
                                                                                                   tCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCCCCTTCGGCCAATCGCAC‑ATGCCATGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCCGCGCCCCAACCGCCGCTAGCGCCCTCATCACCGTccacca                                                     >  2:5549068/1‑151 (MQ=25)
                                                                                                     cAAAACACCGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGt                                                   <  2:2352545/151‑1 (MQ=255)
                                                                                                     cAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGt                                                   <  2:2834908/151‑1 (MQ=255)
                                                                                                        aaCACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCAATCGCTC‑ATGCCTTGCGCTCCCGGCCCGCGGCGCGGCCCGCCCCTCAAGGCGGCGCCCCAACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGAt                                                >  2:7150371/1‑151 (MQ=255)
                                                                                                           acaGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGACTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCATACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCa                                             <  1:6598024/151‑1 (MQ=255)
                                                                                                           acaGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCGATCGCTC‑ATGCCGTGCGCTCCCCGCCGGCGGCGCGGCCCCCCCCACAAGGCGGCGCCCCAACCCCCGCGAGCGCCGGCCTCACCCTCCACCCCTGATGCa                                             >  2:2967438/1‑151 (MQ=18)
                                                                                                                  gTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCgccca                                      <  1:1452023/151‑1 (MQ=255)
                                                                                                                   tttATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCgcccag                                     <  1:9334503/151‑1 (MQ=255)
                                                                                                                      aTCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCgcccagccc                                  <  1:2262362/151‑1 (MQ=255)
                                                                                                                       tCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCgcccagccca                                 <  2:5755192/151‑1 (MQ=255)
                                                                                                                         tGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATc                               <  2:7930521/151‑1 (MQ=255)
                                                                                                                         tGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTAACGCGC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCGCCTGGCGGCGCCCCAACCGCCGCTAGCGCCGGCCTCACCCTCCCCCAGGGATGCCGCgcccagcccaag                               >  1:8829842/1‑149 (MQ=21)
                                                                                                                                 tACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCgg                       <  2:2649065/151‑1 (MQ=255)
                                                                                                                                       ggCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAg                 <  2:2602964/151‑1 (MQ=255)
                                                                                                                                       ggCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAg                 <  1:2120574/151‑1 (MQ=255)
                                                                                                                                          gTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGaa              >  2:10353009/1‑151 (MQ=255)
                                                                                                                                            cGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAAtt            <  1:8679706/151‑1 (MQ=255)
                                                                                                                                                cgcgCCTTCGGCCTCTCGCTC‑ATGCCTAGCGCGCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCCCCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAATTCCCa        <  2:7307678/151‑1 (MQ=255)
                                                                                                                                                cgcgCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAATTCCCa        <  1:9419353/151‑1 (MQ=255)
                                                                                                                                                 gcgcCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAATTCCCaa       >  2:1577217/1‑151 (MQ=255)
                                                                                                                                                  cgcCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAATTCCCAAt      >  1:3300406/1‑151 (MQ=255)
                                                                                                                                                  cgcCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAATTCCCAAt      <  2:3799732/151‑1 (MQ=255)
                                                                                                                                                     cTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAATTCCCAATCGa   >  1:72255/1‑151 (MQ=255)
                                                                                                                                                      ttCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGACTTCCCAATCgct  >  2:6300404/1‑149 (MQ=255)
                                                                                                                                                      ttCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAATTCCCAATCGAt  >  2:7745346/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
GGGGGAGTCCAGCAGTTGTCCGTCGGGTTGCAACAGGAGGCGGTTTTGATGAATCGGTTTCACTTTCTTCATGGGCTTACCCAGCAATGTCCATTAATGTCCAAAACACAGGGCGTTTATCTGACGTGTTACAGGGGCGTCGGCCGCGCCTTCGGCCTATCGCTC‑ATGCCTTGCGCTCCCGGCCGGCGGCGCGGCCCGCCCCTCATGGCGGCGCCCCTACCGCCGCTAGCGCCGTCATCACCGTCCACCAGTGATGCAGCGCCCAGCCCATCGCCACCGGAACGAGGAATTCCCAATCGAT  >  NC_008463/4347430‑4347730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑