Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,829,3150TG29.1% 949.1 / 101.9 388K8T (AAA→ACA) PA14_RS22165succinate dehydrogenase assembly factor 2
Reads supporting (aligned to +/- strand):  ref base T (55/220);  new base G (113/0);  total (168/220)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 397 total aligned reads displayed.

CAACCGAACATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTATCCGC  >  NC_008463/4829165‑4829465
                                                                                                                                                      |                                                                                                                                                      
   ccGAACCTGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCa                                                                                                                                                     <  2:907890/151‑1 (MQ=255)
    cGAACATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAg                                                                                                                                                    >  2:7253716/1‑151 (MQ=255)
     gAACATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGt                                                                                                                                                   <  2:1110966/151‑1 (MQ=255)
      aaCATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGtt                                                                                                                                                  >  2:5497557/1‑151 (MQ=255)
       aCATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGttgaaggtg                                                                                                                                                 >  1:2244023/1‑144 (MQ=255)
       aCATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTc                                                                                                                                                 <  1:623167/151‑1 (MQ=255)
        cATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCg                                                                                                                                                <  1:1048237/151‑1 (MQ=255)
        cATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCg                                                                                                                                                <  1:2498891/151‑1 (MQ=255)
          tGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAACGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGt                                                                                                                                              <  2:6684042/151‑1 (MQ=255)
          tGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGt                                                                                                                                              >  2:7040867/1‑151 (MQ=255)
          tGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGt                                                                                                                                              <  2:587520/151‑1 (MQ=255)
           gTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGGTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGtt                                                                                                                                             <  2:9156208/151‑1 (MQ=255)
             ccTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTc                                                                                                                                           <  1:10059837/151‑1 (MQ=255)
              cTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTCGGTTTCa                                                                                                                                          >  2:8868514/1‑151 (MQ=255)
                ggTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATc                                                                                                                                        <  2:7376387/151‑1 (MQ=255)
                       gCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATg                                                                                                                                 <  1:6648003/151‑1 (MQ=255)
                        cACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTAGGTTTGAACTGCCATGt                                                                                                                                >  2:8455427/1‑151 (MQ=255)
                         aCTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGtt                                                                                                                               <  2:8544329/151‑1 (MQ=255)
                           tCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCg                                                                                                                             <  2:9178974/151‑1 (MQ=255)
                           tCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCg                                                                                                                             <  2:2013839/151‑1 (MQ=255)
                              agcagcTAGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGc                                                                                                                          <  1:2288946/151‑1 (MQ=255)
                                  gcTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGctcct                                                                                                                      <  1:8151451/151‑1 (MQ=255)
                                  gcTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTAAGTTAGGTTTGATCAGCCATGTTCCGGctcca                                                                                                                      >  2:7633096/1‑150 (MQ=255)
                                        ggAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGaaaa                                                                                                                <  1:3157974/151‑1 (MQ=255)
                                         gAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCTGTTCGGGTTAATAATCCATGTTAGGGCGCCTCGAAAAc                                                                                                               >  2:8647755/1‑151 (MQ=255)
                                         gAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAAc                                                                                                               <  1:5269731/151‑1 (MQ=255)
                                              gcgcCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCg                                                                                                          <  2:10593313/151‑1 (MQ=255)
                                              gcgcCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCAGTGCCAGTAGAGTCGTTTGGGTTCGGTGTGGTATGCCCTGGTCGGGGGCCGCGAGAAAGCCCg                                                                                                          >  1:9162499/1‑151 (MQ=255)
                                                     gTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCATTTAGGTTTCATAAGCCCATTTAGGGCAACTAGAAAACCCCCGGCAatat                                                                                                   >  2:1472894/1‑151 (MQ=255)
                                                      tCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATtata                                                                                                  <  1:5586926/151‑1 (MQ=255)
                                                       ccTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAg                                                                                                 <  2:872821/151‑1 (MQ=255)
                                                          cGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGc                                                                                              <  2:4815814/151‑1 (MQ=255)
                                                             cGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGt                                                                                           <  1:9369886/151‑1 (MQ=255)
                                                              gTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGGTCCTCGAAAACGCCCGGCATTATAGGGGGGtt                                                                                          >  2:3380565/1‑151 (MQ=255)
                                                              gTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTAGGGTTAATATTCCATGTTCGGGCTACACGAAAACGCCCGGCAATATAGTGGGGtt                                                                                          >  2:2263341/1‑151 (MQ=255)
                                                               tCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGGTAGGTTTCAACTGCCAAGTTAGGGCTCCTCGAAAAAGCCCGGCCTGATAGTGCGGTTg                                                                                         >  1:9587663/1‑151 (MQ=255)
                                                                 cAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTgcg                                                                                       <  1:2135915/151‑1 (MQ=255)
                                                                 cAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTAAGTTAGGGTTGAACTTCCAAGTTAGGGGTCCTCGAAAAAGCCCGGCCTTATTTGGCGGtttgg                                                                                       >  2:4298671/1‑148 (MQ=255)
                                                                  aGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTGAGTTAGGTTTCATCTGCCAAGTTCGGGGTCCGAGAAAAAGCCCGGCCTTATTGGGGGGGTgggc                                                                                      >  2:1572677/1‑151 (MQ=255)
                                                                     cccGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCTGGTCGGTTTAATATTCCCAGGTAGGGGGCCTCGCAAACGCCCGGCCTTTTAGGGCGGGTGGGCTCg                                                                                   >  2:2430828/1‑151 (MQ=255)
                                                                      ccGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTAAGTTAGGTTTCATATTCCAAGTTAGGGATCCACCAAAACGCCCGGGATTATTTGGCGGTTGCGCACGa                                                                                  >  2:2584146/1‑151 (MQ=255)
                                                                        gggTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAAt                                                                                <  2:7574864/151‑1 (MQ=255)
                                                                         ggTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTCGGGTTCATATGCCAAGTTCGGGCTCCTCGAAAACGCCCCGCAATATAGGGCGGTTGCGCTCGaaag                                                                               >  2:10711947/1‑149 (MQ=255)
                                                                           tAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTAGGGTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTTTAGGGCGGGTGCGCTCGCATGgc                                                                             >  2:6806622/1‑151 (MQ=255)
                                                                              aCCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGcgcg                                                                          <  1:3399616/151‑1 (MQ=255)
                                                                              aCCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGcgcg                                                                          <  2:79466/151‑1 (MQ=255)
                                                                                ctcctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTAAGTTCGGTTTAAACTGCCAGGTTAGGGCTCCTCGAAAAAGCCCGGCAGTATATGGGGGTTTCGCGCGCAAGGCGCGGc                                                                        >  2:4916651/1‑151 (MQ=255)
                                                                                ctcctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGGTAGGGTTCATCTGCCATGGTCGGGGGCCTCGAAAAAGCCCGGCCGGATAGGGCGGTTGCGCTCGAAAGGggcggc                                                                        >  2:3812355/1‑151 (MQ=255)
                                                                                 tcctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGcc                                                                       <  1:10266660/151‑1 (MQ=255)
                                                                                   ctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGGGGTCGGGTTGATCAGCCAAGGTGGGGGTCCACGAAAAACCCCGCCAATATATGGCGGTTGGGCTCGAATTGCGCGGcccc                                                                     >  1:372232/1‑150 (MQ=255)
                                                                                       aCGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGGTAGGGTTAATCGGCCATGTTAGGGCGCCGCCCAAAACCCCCGCCTTATAGGGCGGGTGCGCGCGAATTGGGCGGCCCGAAGa                                                                 >  1:7500620/1‑151 (MQ=255)
                                                                                        cGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTCGGGTTGAACTGGCAGGGTAGGGCGCCGCGGAAACCCCCCGCCGTATATGGCGGTTGGGCTCGGATTGCGCGGcccccttag                                                                >  1:2609832/1‑145 (MQ=255)
                                                                                        cGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTCGGTTTCATCTGCCCAGTTCGGGCTCCACGAAAACGCCCGGCCTTATAGGGCGGTTGAGCACGAATTGCGCGGCCCCAAGAc                                                                >  2:2515349/1‑151 (MQ=255)
                                                                                         gAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTGAGTTGGGTTTAATAATCCAATTTGGGGCGACAAGAAAAAGCCCGGCCTTATATGGCGGGTTCGCACGTATTGCGCGGccccaatacg                                                               >  2:8044725/1‑144 (MQ=255)
                                                                                         gAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTAAGGTCGGTTTGATATGCCAGGGTAGGGCTCCGCGAAAACGCCCGGCCGTATAGGGCGGGTTCGCGCGAATTGCGCGGCCCGATGACg                                                               >  2:2916544/1‑151 (MQ=255)
                                                                                          aaaGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTTGGTTTAATCTGCCATGTTGTGGGGCCGCGCAACCGCCCGGCCTTATCGGGGGGTTTGGGGCGCATGGCGCGGGCCGATGACgg                                                              >  2:6546048/1‑150 (MQ=255)
                                                                                           aaGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAg                                                             <  1:8435266/151‑1 (MQ=255)
                                                                                           aaGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAg                                                             <  1:5460916/151‑1 (MQ=255)
                                                                                           aaGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAg                                                             <  1:1496831/151‑1 (MQ=255)
                                                                                           aaGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAg                                                             <  1:3027554/151‑1 (MQ=255)
                                                                                           aaGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAg                                                             <  1:6907118/151‑1 (MQ=255)
                                                                                           aaGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTCGGTTTGATATGCCAAGTGCGGGCTCCTCGAAAACCCCCCGCATTATATGGCGGTTGGGCTCGAATGGCGCGGCCCGaagtccag                                                             >  2:2161770/1‑144 (MQ=255)
                                                                                            aGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAgg                                                            <  2:8281698/151‑1 (MQ=255)
                                                                                             ggCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGc                                                           <  1:6207581/151‑1 (MQ=255)
                                                                                              gCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCg                                                          <  2:5281500/151‑1 (MQ=255)
                                                                                               cACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTGAGTTGGGTTTAATCAGCCCAGATAGGGCACCACGAAAACCCCCCGCCATATAGGGCGGGTGCGCACCAAAGGCGCGGCCCCATGAAGAGGCGt                                                         >  1:6980411/1‑151 (MQ=255)
                                                                                                aCCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTc                                                        <  1:5954201/151‑1 (MQ=255)
                                                                                                 ccAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCg                                                       <  1:4194812/151‑1 (MQ=255)
                                                                                                    gcagcaCATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTGGGGTTAATCAGCCCAGTTAGGGCTCCGACAAAAAGCCCCGCATTATAGGGGGGTTTCGCTCCAAAGGCGCGGCCCGAAGTCCAGGCGgcgcac                                                    >  2:3592929/1‑149 (MQ=255)
                                                                                                    gcagcaCATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTAAGTTAGGTTTAAAAAGCCAATTTCGGGCGCCCCGAAAAAGCCCCGCATTATAAGGCGGTTGCGCACGAAATGCGCGGCCCGCTGACCAAGCGTCGcga                                                    >  2:9407847/1‑149 (MQ=255)
                                                                                                     cagcaCATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTcc                                                   <  2:7821023/151‑1 (MQ=255)
                                                                                                      agcaCATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGGTTAAACTGCCCTGTTAGGGCTACTAGAAAAAGCCCGGCATTATAGGGCGGTTGAGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTccg                                                  >  2:750110/1‑151 (MQ=255)
                                                                                                      agcaCATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCATTTGGGTTTCATCTGGCAATTTAGGGCACCACCCAAAAGCCCGGCCATATAGGGCGGGTGGGCTCGAATGGCCCGGCCCCAAGACGTTGCGGCGCAccg                                                  >  2:6125181/1‑151 (MQ=255)
                                                                                                          caTCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCgg                                              <  1:7943068/151‑1 (MQ=255)
                                                                                                           aTCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGc                                             <  1:6787880/151‑1 (MQ=255)
                                                                                                           aTCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGc                                             <  1:1162871/151‑1 (MQ=255)
                                                                                                              cAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGCGTTCGGTTTGATCTGCCATGTTAGGGCTCCTCGAAAACGCCCCGCCTTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCCATGAAGAGGCGGCGCTCCCCCGGCGTc                                          >  2:5656539/1‑151 (MQ=255)
                                                                                                               aGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTcc                                         <  1:5699377/151‑1 (MQ=255)
                                                                                                                     aGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCtt                                   <  1:7178215/151‑1 (MQ=255)
                                                                                                                     aGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCtt                                   <  2:1671046/151‑1 (MQ=255)
                                                                                                                     aGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTGGGGTTGAACTTCCATGTTCGGGGGCCTCGAAAACGCCCGGCATTATTGGGGGGGTGCGCGCGAATGGCGCGGGCCGCTGTCGTGGGGGGGCTCCGCCGGCGGCCGcccct                                   >  2:9744436/1‑149 (MQ=255)
                                                                                                                       cATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTgg                                 <  2:2706545/151‑1 (MQ=255)
                                                                                                                       cATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTAAGTTAGGTTTCATATGCCCAGTTAGGGGTCCGCGAAAAAACCCCGCAATATAGGGCGGGTGGGCGCGAATTGCGCGGCCCCCAGTCGAGGCGGCGCTCCCCCCGCGTACGcccaggg                                 >  2:4576232/1‑147 (MQ=255)
                                                                                                                        aTGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGt                                <  2:6081733/151‑1 (MQ=255)
                                                                                                                        aTGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTCGGTTTGCAAGGCCCAGTTGGGGCGCCACGAAAAAGCCCCGCAAGATAGGGCGGTTGGGCGCGAATGGCGCGGCCCCCGGACGCGGCGTCGCGCCCCCCGCGGCCGCCCGTGGt                                >  2:1796324/1‑151 (MQ=255)
                                                                                                                        aTGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTCGGTGTCATCTGCCAAGTTCGGGCTCCACCAAAAAGCCCGGCATGATTGGGCGGTTGGGCGCGAAAGGCGCGGCCCCAAGAAGAGGCGTCGCACCGCCGGCGGCCCCCCGTGGt                                >  2:2619859/1‑151 (MQ=255)
                                                                                                                         tGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTc                               <  1:5075824/151‑1 (MQ=255)
                                                                                                                          gCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCg                              <  1:9738418/151‑1 (MQ=255)
                                                                                                                             gcgACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTAt                           <  2:4710590/151‑1 (MQ=255)
                                                                                                                             gcgACGGCTGTGCCAGTAGAGTCGTGTAAGTTGGGTTTAAACTTCCAATTTAGGGCACCTCGTAAACCCCCCGCAATATAGGGCCGGTTCGCGCGAATTGGGCGGCCCGATGACCACGCGGAGCTACGCCGGCGTCCCCCCATgggccttt                           >  2:4012139/1‑145 (MQ=255)
                                                                                                                                     tgtgCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGc                   <  1:6502401/151‑1 (MQ=255)
                                                                                                                                      gtgCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCa                  <  1:4891758/151‑1 (MQ=255)
                                                                                                                                        gCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCaaa                <  1:2866579/151‑1 (MQ=255)
                                                                                                                                              agagTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAgg          <  1:1074343/151‑1 (MQ=255)
                                                                                                                                               gagTCGTTTCAGTTAGGTTTCATATGCCAAGTTCGGGCTCCTCGAAAACGCCCGGCAATATATGGCGGTTGCGCACGAATTGCGCGGCCCCATTACCAGGCGTCGCTCCGCCGGCGTCCGCCCATGGTCGTAATTCCCGGCAAACCAAggg         >  2:10269837/1‑151 (MQ=255)
                                                                                                                                                agTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGt        <  1:1732897/151‑1 (MQ=255)
                                                                                                                                                  tCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTAt      >  2:9556234/1‑151 (MQ=255)
                                                                                                                                                  tCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTAt      <  2:6429105/151‑1 (MQ=255)
                                                                                                                                                     tttCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTATCcg   <  1:8799239/151‑1 (MQ=255)
                                                                                                                                                     tttCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTATCcg   <  2:10398474/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CAACCGAACATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTATCCGC  >  NC_008463/4829165‑4829465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑