Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,198,441 T→G A547A (GCA→GCC)  PA14_RS14615 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,198,4410TG100.0% 1666.4 / NA 425A547A (GCA→GCC) PA14_RS14615acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (221/204);  total (221/204)

Only 100 of 427 total aligned reads displayed.

TCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAG  >  NC_008463/3198294‑3198587
                                                                                                                                                   |                                                                                                                                                  
 cAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGt                                                                                                                                                >  2:8288911/1‑151 (MQ=255)
 cAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGt                                                                                                                                                <  2:5519954/151‑1 (MQ=255)
        aaaCGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGc                                                                                                                                         >  2:3851089/1‑151 (MQ=255)
            ggTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcc                                                                                                                                     <  1:10199057/151‑1 (MQ=255)
             gTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcct                                                                                                                                    <  2:2572737/151‑1 (MQ=255)
              tttCGGCGCGCCACCACTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcctc                                                                                                                                   <  1:8117930/151‑1 (MQ=255)
                tCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcctcct                                                                                                                                 >  1:10326241/1‑151 (MQ=255)
                    cgcgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTg                                                                                                                             >  1:2038878/1‑151 (MQ=255)
                    cgcgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTg                                                                                                                             >  2:5674218/1‑151 (MQ=255)
                      cgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGcc                                                                                                                           <  1:10735161/151‑1 (MQ=255)
                        ccaccaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAGCCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCgg                                                                                                                         >  2:7997690/1‑151 (MQ=255)
                        ccaccaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCgg                                                                                                                         >  1:3250902/1‑151 (MQ=255)
                        ccaccaGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCgg                                                                                                                         >  2:2959030/1‑151 (MQ=255)
                            caGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGccc                                                                                                                     <  2:2422092/151‑1 (MQ=255)
                              gTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCAc                                                                                                                   >  1:4534327/1‑151 (MQ=255)
                              gTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCAc                                                                                                                   >  1:6251078/1‑151 (MQ=255)
                                 gCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcg                                                                                                                >  1:6936719/1‑151 (MQ=255)
                                     gacccGGTCACTGGCGCGGATACGTGCGTCTAGCTGGCTGGACATGGGACTCCTCGGGCAACCTAGTTGTTGTTATTGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCTCTCGCGCa                                                                                                            <  1:5406624/148‑1 (MQ=255)
                                     gAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCa                                                                                                            >  1:8345154/1‑151 (MQ=255)
                                       aCCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAgg                                                                                                          <  2:8634734/151‑1 (MQ=255)
                                         cGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                        >  1:3017961/1‑151 (MQ=255)
                                         cGGGCACTGGCGCGGATACGTGCGTAGAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                        <  2:2852103/151‑1 (MQ=255)
                                             cACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTCGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAAc                                                                                                    >  2:4465248/1‑151 (MQ=255)
                                              aCTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTCTGGACTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACt                                                                                                   >  2:483220/1‑151 (MQ=255)
                                              aCTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGACCt                                                                                                   >  1:6488076/1‑151 (MQ=255)
                                                  gcgcGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTg                                                                                               <  1:1335916/151‑1 (MQ=255)
                                                  gcgcGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCGAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTg                                                                                               <  1:9198326/151‑1 (MQ=255)
                                                   cgcgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTgg                                                                                              >  2:10252221/1‑151 (MQ=255)
                                                     cgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGAt                                                                                            >  2:6735458/1‑151 (MQ=255)
                                                     cgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGAt                                                                                            <  2:7185588/151‑1 (MQ=255)
                                                     cgGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGAt                                                                                            >  1:5720750/1‑151 (MQ=255)
                                                      ggATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCGCTCGCGCAGGACGAACTGCTGGagc                                                                                           >  2:4068436/1‑149 (MQ=255)
                                                      ggATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATc                                                                                           >  2:2136061/1‑151 (MQ=255)
                                                       gATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCt                                                                                          >  2:1304672/1‑151 (MQ=255)
                                                         tACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTg                                                                                        >  2:5951729/1‑151 (MQ=255)
                                                         tACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTg                                                                                        >  2:9719956/1‑151 (MQ=255)
                                                         tACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTg                                                                                        >  1:6093145/1‑151 (MQ=255)
                                                          aCGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGc                                                                                       <  2:4643822/151‑1 (MQ=255)
                                                           cGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGcc                                                                                      >  2:917443/1‑151 (MQ=255)
                                                            gTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCg                                                                                     <  1:7390159/151‑1 (MQ=255)
                                                             tGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCgg                                                                                    >  1:3929214/1‑151 (MQ=255)
                                                               cGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTg                                                                                  <  1:4748330/151‑1 (MQ=255)
                                                               cGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTg                                                                                  <  2:8908559/151‑1 (MQ=255)
                                                                 tataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGc                                                                                >  2:10593872/1‑151 (MQ=255)
                                                                  ataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCg                                                                               <  1:8846035/151‑1 (MQ=255)
                                                                     gCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTc                                                                            >  1:177967/1‑151 (MQ=255)
                                                                      cAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCt                                                                           <  1:3758881/151‑1 (MQ=255)
                                                                       aGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCtt                                                                          <  2:5430874/151‑1 (MQ=255)
                                                                         gCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTgg                                                                        <  1:9471723/151‑1 (MQ=255)
                                                                            ggACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCa                                                                     >  2:8719220/1‑151 (MQ=255)
                                                                             gACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAg                                                                    <  1:2121920/151‑1 (MQ=255)
                                                                               cATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCGTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGtt                                                                  >  1:4948272/1‑151 (MQ=255)
                                                                                aTGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTc                                                                 >  2:3525491/1‑151 (MQ=255)
                                                                                   ggACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGt                                                              <  2:5045468/151‑1 (MQ=255)
                                                                                    gACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTa                                                             >  1:5494469/1‑151 (MQ=255)
                                                                                    gACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTa                                                             >  2:2478732/1‑151 (MQ=255)
                                                                                      cacCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAg                                                           <  1:4262838/151‑1 (MQ=255)
                                                                                       acCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGc                                                          <  1:9327477/151‑1 (MQ=255)
                                                                                       acCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGc                                                          >  1:8291908/1‑151 (MQ=255)
                                                                                        ccTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCg                                                         >  2:328965/1‑151 (MQ=255)
                                                                                        ccTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCg                                                         >  1:5679108/1‑151 (MQ=255)
                                                                                         cTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGt                                                        <  1:2319772/151‑1 (MQ=255)
                                                                                           cGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTca                                                      >  1:9908023/1‑151 (MQ=255)
                                                                                            gggCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTcac                                                     >  2:7555877/1‑151 (MQ=255)
                                                                                              gCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACAt                                                   <  2:7747275/151‑1 (MQ=255)
                                                                                              gCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACAt                                                   <  1:2462788/151‑1 (MQ=255)
                                                                                                aaCCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGc                                                 >  1:4897002/1‑151 (MQ=255)
                                                                                                     aGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcgg                                            <  1:10597388/151‑1 (MQ=255)
                                                                                                      gTTAGTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcggc                                           <  1:1929726/151‑1 (MQ=255)
                                                                                                       ttATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcggcg                                          <  1:2698637/151‑1 (MQ=255)
                                                                                                       ttAGTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcggcg                                          >  2:10524404/1‑151 (MQ=255)
                                                                                                          ttGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGccg                                       >  2:6135582/1‑150 (MQ=255)
                                                                                                           tGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCtt                                      >  2:6267640/1‑151 (MQ=255)
                                                                                                              tATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGaa                                   <  1:3492177/151‑1 (MQ=255)
                                                                                                               aTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAg                                  >  2:9487065/1‑151 (MQ=255)
                                                                                                                 ggAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTg                                >  2:8141672/1‑151 (MQ=255)
                                                                                                                   aaTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTgcg                              >  2:1470805/1‑151 (MQ=255)
                                                                                                                     tgtgCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGcc                            >  1:252811/1‑151 (MQ=255)
                                                                                                                     tgtgCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGcc                            <  1:681338/151‑1 (MQ=255)
                                                                                                                      gtgcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCa                           <  1:7704886/151‑1 (MQ=255)
                                                                                                                       tgcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAg                          >  2:8515464/1‑151 (MQ=255)
                                                                                                                        gcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAgg                         <  1:8440994/151‑1 (MQ=255)
                                                                                                                         cgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGgc                        <  1:121092/151‑1 (MQ=255)
                                                                                                                          gggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACGTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGgcg                       >  1:7160438/1‑151 (MQ=255)
                                                                                                                             tgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTc                    <  2:5830721/151‑1 (MQ=255)
                                                                                                                             tgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTc                    <  2:5361765/151‑1 (MQ=255)
                                                                                                                              gcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTcg                   <  2:1710530/151‑1 (MQ=255)
                                                                                                                              gcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTcg                   <  1:10017696/151‑1 (MQ=255)
                                                                                                                                  cAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGc               <  2:10064191/151‑1 (MQ=255)
                                                                                                                                  cAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTACTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGc               >  1:5293186/1‑151 (MQ=255)
                                                                                                                                   aGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCa              >  1:891654/1‑151 (MQ=255)
                                                                                                                                     cgcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCAcc            >  2:6408159/1‑151 (MQ=255)
                                                                                                                                     cgcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCAcc            <  2:4754089/151‑1 (MQ=255)
                                                                                                                                     cgcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCAcc            <  2:1210174/151‑1 (MQ=255)
                                                                                                                                      gcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGCTCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCa           >  2:1577376/1‑151 (MQ=255)
                                                                                                                                      gcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCa           >  2:5201859/1‑151 (MQ=255)
                                                                                                                                       ccATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAg          <  1:2912436/151‑1 (MQ=255)
                                                                                                                                           cAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGa      <  1:5123811/151‑1 (MQ=255)
                                                                                                                                            aGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGAc     >  1:8744126/1‑151 (MQ=255)
                                                                                                                                               ggCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAg  >  2:9424156/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAG  >  NC_008463/3198294‑3198587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑