Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,423,281 A→G K130K (AAA→AAG)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,423,2810AG98.8% 1589.5 / NA 485K130K (AAA→AAG) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (253/226);  minor base T (6/0);  total (259/226)
Fisher's exact test for biased strand distribution p-value = 3.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 488 total aligned reads displayed.

CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGCC  >  NC_008463/3423133‑3423428
                                                                                                                                                    |                                                                                                                                                   
ccaGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGTCGAGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGCGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgc                                                                                                                                                   <  2:7634956/151‑1 (MQ=255)
ccaGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgc                                                                                                                                                   <  1:2810511/151‑1 (MQ=255)
  agcagcACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCGACGTGGAGAcggcgg                                                                                                                                                 <  2:7473522/151‑1 (MQ=255)
   gcagcaCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGATGATCGTCGAGTCGCGCCTGCCCGTGTGGGTGCGGCGCGTCGGGATCTACGTGGAGGAggcggc                                                                                                                                                >  1:6253035/1‑151 (MQ=255)
    cagcaCCGGCCAGGCGTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGgcggcg                                                                                                                                               <  1:7308426/151‑1 (MQ=255)
        aCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGGATCTACGTGGAGAAGGCGGCGGAcc                                                                                                                                           >  2:5723200/1‑150 (MQ=255)
         ccGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAg                                                                                                                                          <  2:1262384/151‑1 (MQ=255)
         ccGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAg                                                                                                                                          <  1:5869339/151‑1 (MQ=255)
         ccGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGAccg                                                                                                                                          >  1:8628209/1‑149 (MQ=255)
                ggCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAg                                                                                                                                   >  2:8349075/1‑151 (MQ=255)
                 gCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGc                                                                                                                                  <  2:7909774/151‑1 (MQ=255)
                    tCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGa                                                                                                                               <  1:2594474/151‑1 (MQ=255)
                      gCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATc                                                                                                                             >  2:9827263/1‑151 (MQ=255)
                       ctgaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGGGGCGCGACGGTGTGTACGTGGAGAAGGCGGCGGACCGCATCCAGCTGATCg                                                                                                                            >  1:8308783/1‑151 (MQ=255)
                         gaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTc                                                                                                                          >  1:6865831/1‑151 (MQ=255)
                          accctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAATGCGGCGGACAGCATCCAGCTGATCGTCa                                                                                                                         >  2:1990007/1‑151 (MQ=255)
                            cctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCAcc                                                                                                                       <  2:7169185/151‑1 (MQ=255)
                            cctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCAcc                                                                                                                       <  1:2309957/151‑1 (MQ=255)
                              tgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCt                                                                                                                     <  2:8079726/151‑1 (MQ=255)
                                accTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCa                                                                                                                   >  1:9581397/1‑151 (MQ=255)
                                 ccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAc                                                                                                                  >  2:7240905/1‑151 (MQ=255)
                                   ttCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAcct                                                                                                                <  2:3996139/151‑1 (MQ=255)
                                      cGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAcctcct                                                                                                             >  2:3485194/1‑151 (MQ=255)
                                         cAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCa                                                                                                          <  1:1589703/151‑1 (MQ=255)
                                         cAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCa                                                                                                          <  2:8435280/151‑1 (MQ=255)
                                           gggCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGCCAGCATCCAGCAGATCGTCACCCTCACCTCCGCCAGc                                                                                                        >  2:2507145/1‑151 (MQ=255)
                                              cGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGc                                                                                                     <  2:143522/151‑1 (MQ=255)
                                               gTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCTGCCCGGGTCGGTGCGGGGCGCCGGTTTATACGTGGAGAATGCGGCGGACAGCAACCCGCATATAGTCACCCGCCCCACCGCCCGCGGcc                                                                                                    >  1:139587/1‑151 (MQ=255)
                                                 gAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGGCACCCTCACCTCCTCCAGCGGCCGc                                                                                                  >  2:4157302/1‑151 (MQ=255)
                                                  aaCGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCt                                                                                                 <  2:6429273/151‑1 (MQ=255)
                                                   aCGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACCGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTa                                                                                                >  2:9168597/1‑151 (MQ=255)
                                                    cGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTac                                                                                               <  1:1951940/151‑1 (MQ=255)
                                                    cGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTac                                                                                               <  1:5702642/151‑1 (MQ=255)
                                                    cGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCGGCCCGCGTCGGGGCGGCGCGACGGTATCTACGGGGGGTATGCGGCGGACCGCATCCAGCGGATCGTCCCCCTCACCTCCTCCAGCGGCCGCTac                                                                                               >  1:5492151/1‑151 (MQ=255)
                                                    cGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCGGCCCGAGTCGGTGCGGCGCGCCGGTATCTACGGGGAGAAGGCGGCGGACAGCATCCCGCTGAGCGTCACCCTCACCTCCTCCAGCGGCCGCTac                                                                                               >  1:7597702/1‑151 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacg                                                                                              <  2:320040/151‑1 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCACACCTCCACCAGCGGCCGCAacg                                                                                              >  1:3467740/1‑151 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCAGCCCGAGTCGGGGCGGCGCGACGGTATCTAAGTGGAGAAGGCGGCGGCCAGCATCCAGCTGATCGTCACCCTCACCTCCGCCAGCGGCCGCTacg                                                                                              >  1:7713860/1‑151 (MQ=255)
                                                       cGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacgac                                                                                            <  2:1794540/151‑1 (MQ=255)
                                                       cGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCGCCTCCAGCGGCCGCTacgac                                                                                            >  2:4581724/1‑151 (MQ=255)
                                                        gACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacgacg                                                                                           <  2:6641538/151‑1 (MQ=255)
                                                          ccTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCCCCCGCACCTCCTCCAGCGGCCGCTACGACGcc                                                                                         >  1:1671518/1‑151 (MQ=255)
                                                           cTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGGCGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCACACCTCCTCCAGCGGCCGCTACGACGCCa                                                                                        >  1:7107306/1‑151 (MQ=255)
                                                           cTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCa                                                                                        <  2:3946591/151‑1 (MQ=255)
                                                             cgccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATg                                                                                      <  2:6718893/151‑1 (MQ=255)
                                                                 gcgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCTCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAAc                                                                                  >  1:4948257/1‑151 (MQ=255)
                                                                 gcgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAAc                                                                                  <  2:4864191/151‑1 (MQ=255)
                                                                 gcgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACCGCATACAGCAGATCGTCACCCACACCTCCACCAGCGGCCGCGACGACGcccaggacc                                                                                  >  1:1105298/1‑144 (MQ=255)
                                                                  cgGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGGGTCGGTGCGGCGCGACGGTCTCTACGTGGTGTAGGCGGCGGGCAGGATCCAGCGGATCGTCACCCTCACCTCGTCCAGCGGCCGCTACGTCGCCATGGagcg                                                                                 >  2:5596877/1‑148 (MQ=255)
                                                                   ggTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTg                                                                                <  1:5213108/151‑1 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGc                                                                             <  2:5063507/151‑1 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCGCCTCCAGCGGCCGCTACGACGCCAAGGAACAGGGc                                                                             >  1:10301390/1‑151 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCCCCAGCCCGAGTCGGGGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCACACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGc                                                                             >  2:892327/1‑151 (MQ=255)
                                                                              aGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGGATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCACCTCCAGCGGCCGCTAAGACGCCAAGGAACTGGGCGAGTTCGc                                                                     >  1:4233095/1‑151 (MQ=255)
                                                                               gAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGcc                                                                    <  1:8821678/151‑1 (MQ=255)
                                                                               gAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGcc                                                                    >  2:6542897/1‑151 (MQ=255)
                                                                               gAACCGCCTGAAGATCGTCGAGGCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCCGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGcc                                                                    >  1:10570071/1‑151 (MQ=255)
                                                                                 cccGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGAACGCCTc                                                                  <  1:4413685/150‑1 (MQ=255)
                                                                                 aCCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTc                                                                  <  1:4758338/151‑1 (MQ=255)
                                                                                  ccGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCGACGACGCCATGGAACTGGGCGAGATCGCCTCg                                                                 <  2:333831/151‑1 (MQ=255)
                                                                                    gCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTc                                                               <  2:10258493/151‑1 (MQ=255)
                                                                                    gCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGGCGGTGCGGCGCGACGGTATCTCCGTGGAGAATGCGGCGGACCGCATCCAGCTGATCGTCACCCTCACCACCGCCATCGGCCGCGACGACGCCATTGAACTTGGCGAGATCGCCGCGta                                                               >  2:4443938/1‑150 (MQ=255)
                                                                                        gAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAACTGATCGTCACCCTCACCGCCTCCAGCGGCCGCGACGACGCCATGGAACTGGGCGAGATAGCCACGGCCAAc                                                           >  1:6080321/1‑151 (MQ=255)
                                                                                         aaGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCACCACCAGCGGCCGCTACGACGCCATGGAACAGGGCGAGATCGCCTCGTCCAACg                                                          >  2:5106399/1‑151 (MQ=255)
                                                                                           gATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACgtg                                                        >  2:822779/1‑151 (MQ=255)
                                                                                            cgcgtcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACgtgt                                                       <  1:10024190/149‑1 (MQ=255)
                                                                                                tcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCa                                                   >  1:10120472/1‑151 (MQ=255)
                                                                                                   aGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgc                                                >  2:2268039/1‑151 (MQ=255)
                                                                                                     tCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgcgc                                              <  2:2611377/151‑1 (MQ=255)
                                                                                                      cgcgCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCt                                             <  1:247277/151‑1 (MQ=255)
                                                                                                         gcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgcg                                          <  2:10651163/151‑1 (MQ=255)
                                                                                                         gcCTGCCCGAGTCGGTGCGGCGCGACGGGATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgcg                                          <  2:2745153/151‑1 (MQ=255)
                                                                                                           cTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGcc                                        >  1:650568/1‑151 (MQ=255)
                                                                                                             gCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCgg                                      >  1:1786594/1‑151 (MQ=255)
                                                                                                                  aGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCCTGGACCTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGa                                 >  2:9918299/1‑151 (MQ=255)
                                                                                                                  aGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGa                                 >  1:9971000/1‑151 (MQ=255)
                                                                                                                  aGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGa                                 <  1:78736/151‑1 (MQ=255)
                                                                                                                   gTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAg                                <  2:9542486/151‑1 (MQ=255)
                                                                                                                      ggTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGc                             >  2:8837073/1‑151 (MQ=255)
                                                                                                                        tGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGt                           >  1:3820906/1‑151 (MQ=255)
                                                                                                                        tGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGt                           <  2:3310930/151‑1 (MQ=255)
                                                                                                                        tGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGt                           >  2:2164984/1‑151 (MQ=255)
                                                                                                                          cggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTgg                         <  1:5312205/151‑1 (MQ=255)
                                                                                                                             cgcgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCa                      >  2:6011062/1‑151 (MQ=255)
                                                                                                                             cgcgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCa                      >  2:7676849/1‑151 (MQ=255)
                                                                                                                              gcgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCaa                     >  2:1173859/1‑151 (MQ=255)
                                                                                                                               cgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAg                    <  1:9003293/151‑1 (MQ=255)
                                                                                                                                gACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAgg                   <  2:5322009/151‑1 (MQ=255)
                                                                                                                                 aCGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGt                  >  1:8931392/1‑151 (MQ=255)
                                                                                                                                    gTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcga               <  1:5100836/151‑3 (MQ=255)
                                                                                                                                      aTCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCgaga             <  1:6641479/151‑1 (MQ=255)
                                                                                                                                      aTCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCgaga             >  2:10420322/1‑151 (MQ=255)
                                                                                                                                        cTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGAcc           >  1:7011369/1‑151 (MQ=255)
                                                                                                                                         tACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGATACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCt          >  2:3674277/1‑151 (MQ=255)
                                                                                                                                            gTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTggg       <  1:9212311/151‑1 (MQ=255)
                                                                                                                                             tGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgggg      <  2:8675506/151‑1 (MQ=255)
                                                                                                                                             tGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgggg      >  2:6282138/1‑151 (MQ=255)
                                                                                                                                             tGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgggg      <  1:3652992/151‑1 (MQ=255)
                                                                                                                                             tGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgggg      >  1:3049772/1‑151 (MQ=255)
                                                                                                                                               gagaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgcg    >  2:5633927/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGCC  >  NC_008463/3423133‑3423428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑