Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,543,662 A→G T494A (ACG→GCG)  PA14_RS16110 → PucR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,543,6620AG100.0% 2106.5 / NA 531T494A (ACG→GCG) PA14_RS16110PucR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (236/295);  total (236/295)

Only 100 of 531 total aligned reads displayed.

GAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCGG  >  NC_008463/3543514‑3543809
                                                                                                                                                    |                                                                                                                                                   
   cgccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgg                                                                                                                                                <  2:434625/151‑1 (MQ=255)
     ccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgggg                                                                                                                                              >  1:998033/1‑151 (MQ=255)
     ccgcGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAgggg                                                                                                                                              >  1:8543267/1‑151 (MQ=255)
      cgcgCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGc                                                                                                                                             >  1:6344419/1‑151 (MQ=255)
      cgcgCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGc                                                                                                                                             <  1:3726404/151‑1 (MQ=255)
       gcgcAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCa                                                                                                                                            <  2:1961959/151‑1 (MQ=255)
          cAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGa                                                                                                                                         >  1:3941540/1‑151 (MQ=255)
           aGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGAc                                                                                                                                        <  1:4849696/151‑1 (MQ=255)
           aGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGAc                                                                                                                                        <  2:4717974/151‑1 (MQ=255)
           aGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGAc                                                                                                                                        <  2:8557101/151‑1 (MQ=255)
            gCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACg                                                                                                                                       <  2:749372/151‑1 (MQ=255)
             cGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACgg                                                                                                                                      >  1:9509626/1‑151 (MQ=255)
                 tacacatacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGaa                                                                                                                                  >  2:9398851/1‑151 (MQ=255)
                 tacacatacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGaa                                                                                                                                  >  1:2874679/1‑151 (MQ=255)
                   cacatacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAaga                                                                                                                                <  1:5609471/151‑1 (MQ=255)
                   cacatacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAaga                                                                                                                                <  1:3606999/151‑1 (MQ=255)
                    acatacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAagag                                                                                                                               <  1:4240964/151‑1 (MQ=255)
                      atccacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagca                                                                                                                             <  1:4114677/148‑1 (MQ=255)
                      atacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagca                                                                                                                             <  1:7983158/151‑1 (MQ=255)
                       tacacaAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGagcag                                                                                                                            <  2:4917545/151‑1 (MQ=255)
                         caccAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGACAGCAGCGGTCGCTCGCTGGTCAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCTACCCGGCTGTCGCCGGAAGCGCGGGGCAGGACGGCGAAGAGCAGcc                                                                                                                          <  2:3871759/151‑1 (MQ=255)
                           caAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctc                                                                                                                        >  2:7770094/1‑151 (MQ=255)
                             aGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctcct                                                                                                                      <  1:915158/151‑1 (MQ=255)
                             aGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctcct                                                                                                                      >  1:1021521/1‑151 (MQ=255)
                             aGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGcctcct                                                                                                                      <  2:2655808/151‑1 (MQ=255)
                               cAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTgg                                                                                                                    <  2:5181454/151‑1 (MQ=255)
                               cAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTgg                                                                                                                    >  2:1437797/1‑151 (MQ=255)
                               cAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTgg                                                                                                                    <  2:7702541/151‑1 (MQ=255)
                                agagCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGa                                                                                                                   >  1:1370896/1‑151 (MQ=255)
                                  agCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGc                                                                                                                 <  2:8001986/151‑1 (MQ=255)
                                  agCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGc                                                                                                                 <  1:10064853/151‑1 (MQ=255)
                                   gCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCa                                                                                                                <  1:5282217/151‑1 (MQ=255)
                                    ccTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAg                                                                                                               >  1:4476310/1‑151 (MQ=255)
                                    ccTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGAAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGTCGGCGAAGAGCAGCCTCCTGGAGCAg                                                                                                               <  1:7119305/151‑1 (MQ=255)
                                     cTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGt                                                                                                              >  2:3174136/1‑151 (MQ=255)
                                      tGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTc                                                                                                             <  1:5106951/151‑1 (MQ=255)
                                      tGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTc                                                                                                             <  1:6494152/151‑1 (MQ=255)
                                          cTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCcgcg                                                                                                         <  2:2564050/151‑1 (MQ=255)
                                          cTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCcgcg                                                                                                         >  2:9786063/1‑151 (MQ=255)
                                             ccGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCAc                                                                                                      <  2:5874503/151‑1 (MQ=255)
                                              cGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCAcc                                                                                                     >  1:8895588/1‑151 (MQ=255)
                                              cGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCAcc                                                                                                     >  2:2143729/1‑151 (MQ=255)
                                                 aTACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATg                                                                                                  <  2:1114051/151‑1 (MQ=255)
                                                 aTACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATg                                                                                                  <  1:8754462/151‑1 (MQ=255)
                                                     gccgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggcgg                                                                                              <  2:3646422/151‑1 (MQ=255)
                                                      ccgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATggcggc                                                                                             <  1:6664581/151‑1 (MQ=255)
                                                       cgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGgcggcg                                                                                            >  2:421101/1‑151 (MQ=255)
                                                       cgcATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGgcggcg                                                                                            <  1:10425711/151‑1 (MQ=255)
                                                         cATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGAc                                                                                          >  2:10450464/1‑151 (MQ=255)
                                                         cATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGAc                                                                                          >  1:10577317/1‑151 (MQ=255)
                                                          aTCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACa                                                                                         >  1:10371339/1‑151 (MQ=255)
                                                            cGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATg                                                                                       >  2:2883532/1‑151 (MQ=255)
                                                             gAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATgg                                                                                      <  2:7384805/151‑1 (MQ=255)
                                                              aGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGa                                                                                     >  2:653941/1‑151 (MQ=255)
                                                              aGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGa                                                                                     >  1:2001514/1‑151 (MQ=255)
                                                              aGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGa                                                                                     >  1:2607128/1‑151 (MQ=255)
                                                                  aaTCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGa                                                                                 <  2:873250/151‑1 (MQ=255)
                                                                    tCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcg                                                                               <  2:4139273/151‑1 (MQ=255)
                                                                    tCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcg                                                                               <  2:430314/151‑1 (MQ=255)
                                                                      aGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCGTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGAcggc                                                                             >  2:10659315/1‑151 (MQ=255)
                                                                         ggTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGa                                                                          <  1:5997637/151‑1 (MQ=255)
                                                                         ggTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGa                                                                          >  1:8196459/1‑151 (MQ=255)
                                                                            cgctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGcc                                                                       <  1:1868236/151‑1 (MQ=255)
                                                                            cgctcgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGcc                                                                       <  1:7270177/151‑1 (MQ=255)
                                                                                cgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGAt                                                                   >  2:9000953/1‑151 (MQ=255)
                                                                                cgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGAt                                                                   >  1:10765023/1‑151 (MQ=255)
                                                                                cgctGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGAt                                                                   <  1:7572029/151‑1 (MQ=255)
                                                                                   tGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATAcc                                                                >  2:6849945/1‑151 (MQ=255)
                                                                                    ggACAGCACCGCCGATGTGGCGACCCTCTGGGTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCt                                                               <  2:9854503/151‑1 (MQ=255)
                                                                                      aCAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTgg                                                             <  2:4464362/151‑1 (MQ=255)
                                                                                      aCAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTgg                                                             <  2:6346782/151‑1 (MQ=255)
                                                                                         gCACCGCCGATGTGGCGACCCTCGGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACAAGGACTGACGGCGGAGCCCGCTACCTGgcgc                                                          <  2:1747827/151‑1 (MQ=255)
                                                                                           aCCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCtt                                                        >  2:2955556/1‑151 (MQ=255)
                                                                                           aCCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCtt                                                        <  1:9742946/151‑1 (MQ=255)
                                                                                            ccgccgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTg                                                       <  1:1777246/151‑1 (MQ=255)
                                                                                              gccgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCa                                                     <  2:7079373/151‑1 (MQ=255)
                                                                                                cgATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAgg                                                   >  1:221171/1‑151 (MQ=255)
                                                                                                 gATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGga                                                  >  1:6250172/1‑151 (MQ=255)
                                                                                                  aTGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgac                                                 <  2:2333464/151‑1 (MQ=255)
                                                                                                   tgtgGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgacg                                                <  2:9541357/151‑1 (MQ=255)
                                                                                                   tgtgGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgacg                                                <  1:9022254/151‑1 (MQ=255)
                                                                                                     tgGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGgacgac                                              <  2:6748575/151‑1 (MQ=255)
                                                                                                        cGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgcc                                           <  1:7329923/151‑1 (MQ=255)
                                                                                                         gACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccg                                          >  1:6967642/1‑151 (MQ=255)
                                                                                                         gACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccg                                          >  2:10303275/1‑151 (MQ=255)
                                                                                                         gACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccg                                          >  1:5867550/1‑151 (MQ=255)
                                                                                                           cccTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgcc                                        <  2:9156007/151‑1 (MQ=255)
                                                                                                            ccTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGAcgccgccg                                       >  2:10127187/1‑151 (MQ=255)
                                                                                                                 ggTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAAc                                  >  1:787835/1‑151 (MQ=255)
                                                                                                                 ggTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAAc                                  <  1:4620315/151‑1 (MQ=255)
                                                                                                                       ccTTGCAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGgcgc                            >  1:3065210/1‑151 (MQ=255)
                                                                                                                            cAGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCa                       <  2:721333/151‑1 (MQ=255)
                                                                                                                             aGGCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAg                      <  2:8416644/151‑1 (MQ=255)
                                                                                                                              ggCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAgg                     <  1:9353904/151‑1 (MQ=255)
                                                                                                                               gCAACCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGa                    <  2:1577129/151‑1 (MQ=255)
                                                                                                                                 aaCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAAc                  <  1:2354936/151‑1 (MQ=255)
                                                                                                                                 aaCCCGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAAc                  >  2:6431145/1‑151 (MQ=255)
                                                                                                                                     cGGCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGtt              <  2:6675104/151‑1 (MQ=255)
                                                                                                                                      ggCTGTCGCCGGAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTg             >  2:5424961/1‑151 (MQ=255)
                                                                                                                                                ggAAGCGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCcgcg   >  2:5549418/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACGCCGCGCAGCGGCTACACATACACAAGCAGAGCCTGGTCTACCGGATACGCCGCATCGAGGAAATCAGCGGTCGCTCGCTGGACAGCACCGCCGATGTGGCGACCCTCTGGTTCGCCTTGCAGGCAACCCGGCTGTCGCCGGAAACGAGGGGCAGGACGGCGAAGAGCAGCCTCCTGGAGCAGTCCGCGCACCATGGCGGCGACATGGACTGACGGCGGAGCCCGATACCTGGCGCTTGCAGGACGACGCCGCCGCCAACAGGCGCGGCCAGGAACGGTTGCCTGGCCGCGG  >  NC_008463/3543514‑3543809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑