Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,997,786 T→C K173E (AAG→GAG)  PA14_RS22840 ← DUF853 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,997,7860TC100.0% 1918.3 / NA 486K173E (AAG→GAG) PA14_RS22840DUF853 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (252/234);  total (252/234)

Only 100 of 486 total aligned reads displayed.

CGCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGC  >  NC_008463/4997638‑4997933
                                                                                                                                                    |                                                                                                                                                   
cgcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGc                                                                                                                                                   >  2:5448753/1‑151 (MQ=255)
 gcCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGcc                                                                                                                                                  >  1:3493325/1‑151 (MQ=255)
  cccccGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCg                                                                                                                                                 <  1:9932913/151‑1 (MQ=255)
  cccccGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCg                                                                                                                                                 <  1:4203843/151‑1 (MQ=255)
  cccccGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCg                                                                                                                                                 >  1:10672774/1‑151 (MQ=255)
  cccccGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCg                                                                                                                                                 >  1:10403532/1‑151 (MQ=255)
     ccgcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAgg                                                                                                                                              >  1:665227/1‑151 (MQ=255)
      cgcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGa                                                                                                                                             <  1:523265/151‑1 (MQ=255)
      cgcccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGa                                                                                                                                             <  1:6148957/151‑1 (MQ=255)
        cccgTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGAtc                                                                                                                                           >  1:3836960/1‑151 (MQ=255)
           gTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTcc                                                                                                                                        >  1:2249352/1‑151 (MQ=255)
            tCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCg                                                                                                                                       >  1:5464275/1‑151 (MQ=255)
            tCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCg                                                                                                                                       >  2:4857494/1‑151 (MQ=255)
                 cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                  >  2:9010283/1‑151 (MQ=255)
                 cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                  >  1:2432131/1‑151 (MQ=255)
                 cTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTg                                                                                                                                  >  2:4487797/1‑151 (MQ=255)
                  tCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGt                                                                                                                                 >  1:232975/1‑151 (MQ=255)
                   cGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTc                                                                                                                                <  2:5203970/151‑1 (MQ=255)
                      ggCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCtt                                                                                                                             <  2:8221975/151‑1 (MQ=255)
                        cGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCa                                                                                                                           <  1:498846/151‑1 (MQ=255)
                             gaTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGTAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAggtgg                                                                                                                      >  1:9813536/1‑151 (MQ=255)
                              aTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAggtggt                                                                                                                     >  2:6875316/1‑151 (MQ=255)
                              aTGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAggtggt                                                                                                                     >  1:7040780/1‑151 (MQ=255)
                                 tcgtCCAGGTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGGGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGa                                                                                                                  <  2:9374089/148‑1 (MQ=255)
                                 tCTTCCAGGTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGa                                                                                                                  <  2:10374870/151‑1 (MQ=255)
                                    tCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagca                                                                                                               <  2:2370793/151‑1 (MQ=255)
                                    tCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagca                                                                                                               <  2:2241029/151‑1 (MQ=255)
                                     ccAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagcag                                                                                                              >  2:3741961/1‑151 (MQ=255)
                                      cAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGagcagc                                                                                                             <  2:1884897/151‑1 (MQ=255)
                                             agggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttca                                                                                                      <  2:7562669/151‑1 (MQ=255)
                                               ggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcagg                                                                                                    >  1:5730761/1‑151 (MQ=255)
                                               ggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcagg                                                                                                    <  2:10185888/151‑1 (MQ=255)
                                               ggcaggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcagg                                                                                                    <  2:3427899/151‑1 (MQ=255)
                                                   ggCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcct                                                                                                >  1:1873431/1‑151 (MQ=255)
                                                    gCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcctt                                                                                               <  2:5753776/151‑1 (MQ=255)
                                                    gCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcctt                                                                                               <  2:8317255/151‑1 (MQ=255)
                                                    gCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtcctt                                                                                               >  1:8681701/1‑151 (MQ=255)
                                                       cGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcag                                                                                            <  2:6021686/151‑1 (MQ=255)
                                                         ccGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGccttcaggtccttcaggt                                                                                          >  1:7187313/1‑151 (MQ=255)
                                                            aagaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGa                                                                                       <  1:2636932/151‑1 (MQ=255)
                                                            aagaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGa                                                                                       <  1:7746530/151‑1 (MQ=255)
                                                             agaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGag                                                                                      >  2:4454716/1‑151 (MQ=255)
                                                              gaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagc                                                                                     >  2:8515264/1‑151 (MQ=255)
                                                              gaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagc                                                                                     >  1:93068/1‑151 (MQ=255)
                                                              gaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagc                                                                                     >  1:9167193/1‑151 (MQ=255)
                                                              gaagGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGAGTGTCCTTCAGGTGGTTGAGCAGCGCCTTCTGGTCCTTCAGGTCGagc                                                                                     >  1:4741922/1‑151 (MQ=255)
                                                                agGCCTCGGCGCCCTGCTGCTCCAGAGGGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGagcag                                                                                   <  2:10672117/151‑1 (MQ=255)
                                                                     tCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGACCAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGGGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAgcagcagca                                                                              <  2:6222088/151‑1 (MQ=255)
                                                                      cGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGcagcagcag                                                                             >  2:7250959/1‑151 (MQ=255)
                                                                       ggCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAgg                                                                            >  1:878976/1‑151 (MQ=255)
                                                                         cgcCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGcc                                                                          >  1:8062708/1‑151 (MQ=255)
                                                                          gcCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCGTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCt                                                                         >  2:7664896/1‑151 (MQ=255)
                                                                             ctgctgCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTcc                                                                      <  1:4370457/151‑1 (MQ=255)
                                                                               gctgctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCg                                                                    <  2:2699472/151‑1 (MQ=255)
                                                                                 tgctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGt                                                                  <  1:857044/151‑1 (MQ=255)
                                                                                 tgcgCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGt                                                                  <  1:625583/151‑1 (MQ=255)
                                                                                  gctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTc                                                                 <  1:10114371/151‑1 (MQ=255)
                                                                                  gctCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTc                                                                 >  1:3734337/1‑151 (MQ=255)
                                                                                    tCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCgg                                                               >  2:2044786/1‑151 (MQ=255)
                                                                                     ccAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGc                                                              >  2:3301898/1‑151 (MQ=255)
                                                                                       agagTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCTGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCa                                                            >  1:9679933/1‑151 (MQ=255)
                                                                                          gTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCt                                                         <  2:303380/151‑1 (MQ=255)
                                                                                           tGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTg                                                        >  1:7938693/1‑151 (MQ=255)
                                                                                                aGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAgg                                                   <  2:1005738/151‑1 (MQ=255)
                                                                                                aGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAgg                                                   <  2:86471/151‑1 (MQ=255)
                                                                                                 ggcggcGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAggc                                                  <  2:6096131/151‑1 (MQ=255)
                                                                                                   cggcgCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAggcgg                                                >  1:3045767/1‑151 (MQ=255)
                                                                                                   cggcgCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAggcgg                                                <  2:121748/151‑1 (MQ=255)
                                                                                                      cgcAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGa                                             >  1:7319863/1‑151 (MQ=255)
                                                                                                      cgcAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGGTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGa                                             >  2:3899719/1‑151 (MQ=255)
                                                                                                         agcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACa                                          <  1:10762722/151‑1 (MQ=255)
                                                                                                         agcagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACa                                          >  1:3158218/1‑151 (MQ=255)
                                                                                                           cagcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgc                                        <  2:9839304/151‑1 (MQ=255)
                                                                                                            agcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcg                                       <  1:1601993/151‑1 (MQ=255)
                                                                                                            agcGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcg                                       <  1:8872734/151‑1 (MQ=255)
                                                                                                             gcgcCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAgcgc                                      >  1:3625311/1‑151 (MQ=255)
                                                                                                               gcCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTGGAGCAGCGCCTTCAGGTCCTGCAGGTCGCGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCgg                                    >  2:7370750/1‑151 (MQ=255)
                                                                                                                ccTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGc                                   <  2:6801899/151‑1 (MQ=255)
                                                                                                                ccTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGc                                   <  1:2034350/151‑1 (MQ=255)
                                                                                                                 cTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGcc                                  <  2:9559275/151‑1 (MQ=255)
                                                                                                                 cTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGcc                                  >  2:5367421/1‑151 (MQ=255)
                                                                                                                  tGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCct                                 <  2:7832032/151‑1 (MQ=255)
                                                                                                                  tGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCct                                 <  2:6518335/151‑1 (MQ=255)
                                                                                                                  tGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGGCGGCCACCTGGAAGGCGGCGAACAGCGCGGCct                                 >  2:2510631/1‑151 (MQ=255)
                                                                                                                    cccGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgc                               <  1:1713264/151‑1 (MQ=255)
                                                                                                                     ccGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgct                              <  2:5562443/151‑1 (MQ=255)
                                                                                                                      cGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCGGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgctg                             >  1:3227957/1‑151 (MQ=255)
                                                                                                                      cGGAAGCGCCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCctgctg                             >  1:1221149/1‑151 (MQ=255)
                                                                                                                            cgcCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGTTTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCGTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATc                       >  1:7150610/1‑151 (MQ=255)
                                                                                                                            cgcCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATc                       <  1:9453973/151‑1 (MQ=255)
                                                                                                                            cgcCGGTGAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATc                       >  2:3567327/1‑151 (MQ=255)
                                                                                                                                   gAACAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAAc                <  2:4303531/151‑1 (MQ=255)
                                                                                                                                      cAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTcc             <  1:10014434/151‑1 (MQ=255)
                                                                                                                                      cAGCGCCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTcc             >  1:1382259/1‑151 (MQ=255)
                                                                                                                                         cgcCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCagg          >  2:10540183/1‑151 (MQ=255)
                                                                                                                                         cgcCCGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCagg          <  2:4099263/151‑1 (MQ=255)
                                                                                                                                            ccGGTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCaggagg       >  2:3228886/1‑151 (MQ=255)
                                                                                                                                               gTCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTg    <  2:3837154/151‑1 (MQ=255)
                                                                                                                                                tCCTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTgg   >  1:6820018/1‑151 (MQ=255)
                                                                                                                                                 ccTCGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGc  >  1:1930811/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCCCCGCCCGTCCGGCTCGGGGCGGAGGATGTCTTCCAGTTGCAGGGCAGGCTCGCCGAAGAAGGCCTCGGCGCCCTGCTGCTCCAGAGTGGCCAGGCGGCGCAGCAGCGCCTGCCCGGAAGCGCCGGTGAACAGCGCCCGGTCCTTGCCGAGGATCTCCGGATTGTCCTTCAGGTGGTTGAGCAGCGCCTTCAGGTCCTTCAGGTCGAGCAGCAGCAGGCCTTCCCGGTCGGCCACCTGGAAGGCGGCGAACAGCGCGGCCTGCTGGCTATCGGTCAACTCCAGGAGGTTGGC  >  NC_008463/4997638‑4997933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑