Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 480,915 T→C K193E (AAA→GAA)  PA14_RS02220 ← 16S rRNA (uracil(1498)‑N(3))‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463480,9150TC99.8% 2071.8 / NA 529K193E (AAA→GAA) PA14_RS0222016S rRNA (uracil(1498)‑N(3))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (266/262);  minor base G (0/1);  total (266/263)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 529 total aligned reads displayed.

TATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCGA  >  NC_008463/480767‑481062
                                                                                                                                                    |                                                                                                                                                   
tatCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCgg                                                                                                                                                   <  2:3479078/151‑1 (MQ=255)
 atCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCggg                                                                                                                                                  >  1:626788/1‑151 (MQ=255)
  tCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGa                                                                                                                                                 <  2:7507472/151‑1 (MQ=255)
   cAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAc                                                                                                                                                >  2:10051826/1‑151 (MQ=255)
    aGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAcc                                                                                                                                               <  1:9068039/151‑1 (MQ=255)
    aGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAcc                                                                                                                                               >  2:1118339/1‑151 (MQ=255)
    aGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAcc                                                                                                                                               >  2:4651108/1‑151 (MQ=255)
      gCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACcca                                                                                                                                             >  1:7733612/1‑151 (MQ=255)
      gCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACcca                                                                                                                                             >  2:4470507/1‑151 (MQ=255)
        gAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccacc                                                                                                                                           >  2:6445387/1‑151 (MQ=255)
         aaGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCACGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccacca                                                                                                                                          >  1:2254528/1‑151 (MQ=255)
          aGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAg                                                                                                                                         >  2:1797307/1‑151 (MQ=255)
             cGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGaa                                                                                                                                      <  1:6373318/151‑1 (MQ=255)
              gCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAg                                                                                                                                     >  2:3792339/1‑151 (MQ=255)
                cccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGc                                                                                                                                   >  1:8880876/1‑151 (MQ=255)
                  cAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCa                                                                                                                                 <  1:10400096/151‑1 (MQ=255)
                  cAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCa                                                                                                                                 >  2:8325662/1‑151 (MQ=255)
                   agagTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAg                                                                                                                                <  2:10570186/151‑1 (MQ=255)
                     agTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgc                                                                                                                              <  1:1525856/151‑1 (MQ=255)
                       ttGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgcgc                                                                                                                            <  2:9552079/151‑1 (MQ=255)
                        tGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGcgcg                                                                                                                           >  1:4955402/1‑151 (MQ=255)
                         gCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGa                                                                                                                          >  2:5139183/1‑151 (MQ=255)
                           tGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcg                                                                                                                        <  2:2490667/151‑1 (MQ=255)
                           tGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcg                                                                                                                        >  1:8403478/1‑151 (MQ=255)
                              gCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcggcg                                                                                                                     <  1:7262183/151‑1 (MQ=255)
                                   cGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCTCCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGcaca                                                                                                                >  1:4377590/1‑151 (MQ=255)
                                        aCGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggct                                                                                                           <  2:8729899/151‑1 (MQ=255)
                                          gCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGACGGCCAGGCGCGACGGCGGCACAtggctgg                                                                                                         >  2:8879629/1‑151 (MQ=255)
                                          gCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggctgg                                                                                                         <  1:7023283/151‑1 (MQ=255)
                                           cTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggctggc                                                                                                        <  1:6818565/151‑1 (MQ=255)
                                            tACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGcc                                                                                                       >  2:1393506/1‑151 (MQ=255)
                                             accaccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCa                                                                                                      >  1:2771328/1‑151 (MQ=255)
                                                accGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAggg                                                                                                   <  2:2863533/151‑1 (MQ=255)
                                                accGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAggg                                                                                                   >  2:8491864/1‑151 (MQ=255)
                                                  cGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAggggg                                                                                                 >  1:2105657/1‑151 (MQ=255)
                                                   ggCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAgggggg                                                                                                <  2:9415656/151‑1 (MQ=255)
                                                     cgcgGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGggcg                                                                                              <  1:5386032/151‑1 (MQ=255)
                                                        ggTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGcc                                                                                           >  2:6797750/1‑151 (MQ=255)
                                                        ggTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGcc                                                                                           >  2:5692462/1‑151 (MQ=255)
                                                          tctcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCca                                                                                          <  1:9983387/150‑1 (MQ=255)
                                                           ctcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCcaca                                                                                        >  1:3067587/1‑151 (MQ=255)
                                                            tcGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAg                                                                                       >  2:6872458/1‑151 (MQ=255)
                                                             cGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCCCAgg                                                                                      >  1:10044728/1‑151 (MQ=255)
                                                             cGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAgg                                                                                      >  1:2394428/1‑151 (MQ=255)
                                                               ctGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGAGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGt                                                                                    <  1:7510751/150‑1 (MQ=255)
                                                                 gcgcAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTGCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGc                                                                                  >  1:5995616/1‑151 (MQ=255)
                                                                   gcagcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAg                                                                                >  1:6525295/1‑151 (MQ=255)
                                                                        aCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCa                                                                           <  2:2739435/151‑1 (MQ=255)
                                                                        aCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCa                                                                           >  2:7045517/1‑151 (MQ=255)
                                                                        aCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCa                                                                           <  2:7871573/151‑1 (MQ=255)
                                                                                 ccGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCt                                                                  >  2:5219357/1‑151 (MQ=255)
                                                                                  cGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTc                                                                 <  2:5923161/151‑1 (MQ=255)
                                                                                   gAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACCTGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCa                                                                >  2:1493331/1‑151 (MQ=255)
                                                                                     ggCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATc                                                              <  1:1314799/151‑1 (MQ=255)
                                                                                        gcgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTc                                                           <  1:3431226/151‑1 (MQ=255)
                                                                                         cgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcc                                                          >  1:1720487/1‑151 (MQ=255)
                                                                                           cggcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccg                                                        >  2:9721412/1‑151 (MQ=255)
                                                                                            ggcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgc                                                       <  1:3964008/151‑1 (MQ=255)
                                                                                             gcgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgcc                                                      <  2:8592751/151‑1 (MQ=255)
                                                                                              cgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgccc                                                     <  1:9599532/151‑1 (MQ=255)
                                                                                              cgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgccc                                                     >  1:9178589/1‑151 (MQ=255)
                                                                                               gTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgcccg                                                    >  1:4853582/1‑151 (MQ=255)
                                                                                                tGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCccgcccgc                                                   >  1:2980958/1‑151 (MQ=255)
                                                                                                tGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCccgcccgc                                                   >  1:5736072/1‑151 (MQ=255)
                                                                                                tGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCccgcccgc                                                   >  1:4290173/1‑151 (MQ=255)
                                                                                                 ggAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCcgcg                                                  >  2:9148763/1‑151 (MQ=255)
                                                                                                    aGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAg                                               <  2:7314147/151‑1 (MQ=255)
                                                                                                    aGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAg                                               <  2:6105123/151‑1 (MQ=255)
                                                                                                       cggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCa                                            <  2:8048326/151‑1 (MQ=255)
                                                                                                        ggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAg                                           >  1:9792806/1‑151 (MQ=255)
                                                                                                        ggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAg                                           <  1:9471383/151‑1 (MQ=255)
                                                                                                        ggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAg                                           >  1:2536651/1‑151 (MQ=255)
                                                                                                          ccgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtc                                         <  2:4803992/151‑1 (MQ=255)
                                                                                                          ccgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtc                                         <  1:7930537/151‑1 (MQ=255)
                                                                                                           cgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcc                                        <  2:3360107/151‑1 (MQ=255)
                                                                                                            gccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcct                                       <  1:5207408/151‑1 (MQ=255)
                                                                                                             ccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcctc                                      <  1:1120721/151‑1 (MQ=255)
                                                                                                                  gCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAggg                                 <  2:548889/151‑1 (MQ=255)
                                                                                                                   ccTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGc                                <  2:535348/151‑1 (MQ=255)
                                                                                                                   ccTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGc                                >  2:3960648/1‑151 (MQ=255)
                                                                                                                    cTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGcc                               <  1:3854433/151‑1 (MQ=255)
                                                                                                                    cTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGcc                               >  1:52929/1‑151 (MQ=255)
                                                                                                                      gCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAg                             <  2:3131007/151‑1 (MQ=255)
                                                                                                                       cTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgc                            <  2:2262605/151‑1 (MQ=255)
                                                                                                                       cTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgc                            <  2:1661775/151‑1 (MQ=255)
                                                                                                                         ccACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgcgg                          <  2:10272335/151‑1 (MQ=255)
                                                                                                                           aCCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgcggcg                        <  1:8212679/151‑1 (MQ=255)
                                                                                                                             cTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCgga                      >  1:4673547/1‑151 (MQ=255)
                                                                                                                              tCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggat                     >  2:5022271/1‑151 (MQ=255)
                                                                                                                              tCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggat                     <  1:6146173/151‑1 (MQ=255)
                                                                                                                                 gCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggatgga                  >  1:8637466/1‑151 (MQ=255)
                                                                                                                                      gCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGc             <  1:4536905/151‑1 (MQ=255)
                                                                                                                                        tCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCCGCGGCGGATGGATCAGCgg           >  1:3787869/1‑151 (MQ=255)
                                                                                                                                        tCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCgg           >  2:3025519/1‑151 (MQ=255)
                                                                                                                                         cAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGc          <  2:9251047/151‑1 (MQ=255)
                                                                                                                                          aGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCa         >  1:5682616/1‑151 (MQ=255)
                                                                                                                                             ccgccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGc      >  1:5934654/1‑151 (MQ=255)
                                                                                                                                             ccgccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGc      >  2:6336273/1‑151 (MQ=255)
                                                                                                                                             ccgccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGc      >  2:8197183/1‑151 (MQ=255)
                                                                                                                                               gccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTc    >  1:10556733/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCGA  >  NC_008463/480767‑481062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑