BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,256,379	C→G	P88A (CCC→GCC)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,256,379	0	C	G	99.5%	1441.1 / NA	370	P88A (CCC→GCC)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (175/193); minor base T (0/2); total (175/195)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 376 total aligned reads displayed.

GGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCAGG  >  NC_008463/5256231‑5256523

ggaggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgcc                                                                                                                                                >  2:8434935/1‑151 (MQ=255)

   ggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgac                                                                                                                                             <  1:7764827/151‑1 (MQ=255)

   ggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgac                                                                                                                                             >  2:248814/1‑151 (MQ=255)

    gaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgaca                                                                                                                                            >  1:1233015/1‑151 (MQ=255)

    gaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgaca                                                                                                                                            <  2:3509799/151‑1 (MQ=255)

        tcttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAacac                                                                                                                                        <  2:3786755/151‑1 (MQ=255)

         cttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACg                                                                                                                                       >  1:8372201/1‑151 (MQ=255)

           tcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGcc                                                                                                                                     <  1:4110995/151‑1 (MQ=255)

               gCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGCTGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTcc                                                                                                                                 <  2:5318049/151‑1 (MQ=255)

               gCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTcc                                                                                                                                 <  2:3464018/151‑1 (MQ=255)

                cATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGATTGATGTCGTGACTGGGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCg                                                                                                                                <  2:10176473/151‑1 (MQ=255)

                  tGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTg                                                                                                                              <  1:617774/151‑1 (MQ=255)

                     gCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGcc                                                                                                                           >  2:7604347/1‑151 (MQ=255)

                       aTCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCaa                                                                                                                         <  2:9584939/151‑1 (MQ=255)

                        tCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAat                                                                                                                        <  2:1998315/151‑1 (MQ=255)

                         cGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAata                                                                                                                       <  2:2813238/151‑1 (MQ=255)

                          ggCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAatat                                                                                                                      <  2:8891576/151‑1 (MQ=255)

                          ggCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAatat                                                                                                                      >  1:8678851/1‑151 (MQ=255)

                             tGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGa                                                                                                                   <  1:4715607/151‑1 (MQ=255)

                             tGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGa                                                                                                                   >  2:6224413/1‑151 (MQ=255)

                                 cTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCt                                                                                                               <  2:4810571/151‑1 (MQ=255)

                                   tCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTa                                                                                                             <  2:7800091/151‑1 (MQ=255)

                                      ccGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAcgc                                                                                                          <  2:239322/151‑1 (MQ=255)

                                        gCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACgcgc                                                                                                        >  2:3330422/1‑151 (MQ=255)

                                        gCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCGTACgcgc                                                                                                        >  1:6527207/1‑151 (MQ=255)

                                           aTCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGa                                                                                                     >  1:3873872/1‑151 (MQ=255)

                                             cAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATc                                                                                                   <  1:6857691/151‑1 (MQ=255)

                                                cGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGTGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGtt                                                                                                >  1:2366092/1‑151 (MQ=255)

                                                 gAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTc                                                                                               >  2:10587502/1‑151 (MQ=255)

                                                  aGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcg                                                                                              >  1:2706230/1‑151 (MQ=255)

                                                   gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcgc                                                                                             <  1:9963735/151‑1 (MQ=255)

                                                     tGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGa                                                                                           >  1:925307/1‑151 (MQ=255)

                                                         cgcgAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCgg                                                                                       >  1:3439800/1‑151 (MQ=255)

                                                           cgAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGc                                                                                     <  1:8973872/151‑1 (MQ=255)

                                                            gAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGca                                                                                    >  1:5882516/1‑151 (MQ=255)

                                                             aaGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGcac                                                                                   >  2:1184439/1‑151 (MQ=255)

                                                                gATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGTCCAATATCGAGGCTTACGCGCAGATAGTTCGCGATCGGGCACAcc                                                                                >  2:9474485/1‑151 (MQ=255)

                                                                 aTGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCt                                                                               <  1:5576355/151‑1 (MQ=255)

                                                                 aTGCACCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCt                                                                               <  2:4063811/151‑1 (MQ=255)

                                                                  tGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTg                                                                              <  1:6311056/151‑1 (MQ=255)

                                                                    cTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCg                                                                            >  2:1552045/1‑151 (MQ=255)

                                                                     tCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCgg                                                                           >  1:3316050/1‑151 (MQ=255)

                                                                      ccGTTTGATGTCGTGACTGTGTCGGCAGCGATCGAAGACCTACCAGAAGCGGGCGGGCTGGCCGACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGACGCTTCCGCGCAGATCGTACGCGATCGGGCACACCTGCGGc                                                                          <  2:1665540/151‑1 (MQ=255)

                                                                         tttGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGc                                                                       >  1:1002626/1‑151 (MQ=255)

                                                                          ttGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCt                                                                      >  2:8371294/1‑151 (MQ=255)

                                                                            ggtGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGa                                                                    >  2:8181262/3‑151 (MQ=255)

                                                                            gATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGa                                                                    >  1:9753924/1‑151 (MQ=255)

                                                                             aTGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCGCCGTGGCCACGATCGAGGCTTACGCGCCGATCGTTCGCGCGCGGGCACACCTGCGGCAGCTGAt                                                                   >  2:9039986/1‑151 (MQ=255)

                                                                               gTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGt                                                                 <  2:6005673/151‑1 (MQ=255)

                                                                                 cGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtct                                                               >  2:3127879/1‑151 (MQ=255)

                                                                                   tGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctct                                                             <  1:2869589/151‑1 (MQ=255)

                                                                                    gACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctctc                                                            <  2:10446580/151‑1 (MQ=255)

                                                                                    gACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctctc                                                            >  1:5155974/1‑151 (MQ=255)

                                                                                    gACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctctc                                                            <  1:1899870/151‑1 (MQ=255)

                                                                                     aCTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCg                                                           <  2:862444/151‑1 (MQ=255)

                                                                                     aCTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCg                                                           <  2:9740247/151‑1 (MQ=255)

                                                                                     aCTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCg                                                           <  1:5977281/151‑1 (MQ=255)

                                                                                       cgtgtCGGAACCGATCGAAGACCTTCCAGAAGCTGGCGGGCGGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCggg                                                         <  2:4318742/150‑1 (MQ=255)

                                                                                         tgtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGca                                                       >  2:9201110/1‑151 (MQ=255)

                                                                                             ggAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCATCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGcaccac                                                   >  1:371723/1‑151 (MQ=255)

                                                                                              gAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACt                                                  <  2:5904702/151‑1 (MQ=255)

                                                                                               aaGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTg                                                 >  1:3175369/1‑151 (MQ=255)

                                                                                               aaGCGATCGAAGACCTTCAAGAAGCTGGCGGGATGGCCTACCTCTGCCAGATCTCCGACATCACGAGCTCAGTTGCCAATATCTAGTCTTACTCTACTATAGGTCGCGATCGGGGACACCTGAGTCAGCTGATGTCTCTCGGGCACCACTg                                                 <  2:399005/151‑1 (MQ=255)

                                                                                                   gATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCAcc                                             >  2:2940240/1‑151 (MQ=255)

                                                                                                   gATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCAcc                                             >  1:718746/1‑151 (MQ=255)

                                                                                                     tCGAAGACCTTCCAGAAGCGGGCGGGCTGGCCGACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAg                                           <  2:9310134/151‑1 (MQ=255)

                                                                                                      cGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAgg                                          <  2:7542956/151‑1 (MQ=255)

                                                                                                      cGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAgg                                          >  1:9436659/1‑151 (MQ=255)

                                                                                                       gAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGa                                         >  1:9651625/1‑151 (MQ=255)

                                                                                                        aaGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAc                                        <  1:6693698/151‑1 (MQ=255)

                                                                                                        aaGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAc                                        <  1:3533848/151‑1 (MQ=255)

                                                                                                         aGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAcc                                       >  2:7143169/1‑151 (MQ=255)

                                                                                                            ccTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGcc                                    <  2:6506154/151‑1 (MQ=255)

                                                                                                              ttCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGCCTCTCGGGCACCACTGCACCAGGACCGCCTc                                  <  2:5967465/151‑1 (MQ=255)

                                                                                                               tCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCg                                 <  1:10622943/151‑1 (MQ=255)

                                                                                                                 cAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGaa                               <  2:1030147/151‑1 (MQ=255)

                                                                                                                 cAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGaa                               >  1:7488435/1‑151 (MQ=255)

                                                                                                                  aGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAac                              <  2:1498008/151‑1 (MQ=255)

                                                                                                                  aGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGAGCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAac                              >  2:2163597/1‑151 (MQ=255)

                                                                                                                    aaGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAacca                            >  1:4369794/1‑151 (MQ=255)

                                                                                                                       cTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAaccacct                         >  2:5079547/1‑150 (MQ=255)

                                                                                                                       cTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAAccacca                         <  1:10339892/151‑1 (MQ=255)

                                                                                                                       cTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCGTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAaccaccc                         <  1:1665008/151‑2 (MQ=255)

                                                                                                                        tGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAg                        <  2:9807887/151‑1 (MQ=255)

                                                                                                                        tGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAg                        <  1:8029013/151‑1 (MQ=255)

                                                                                                                           cGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCa                     >  2:6937255/1‑151 (MQ=255)

                                                                                                                            gggCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCaa                    <  2:6742323/151‑1 (MQ=255)

                                                                                                                            gggCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCaa                    <  2:1910110/151‑1 (MQ=255)

                                                                                                                            gggCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCaa                    >  1:1474149/1‑151 (MQ=255)

                                                                                                                            gggCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACGGCACCAGGACCGCCTCGAACCTCCAGGCaa                    >  2:8290983/1‑151 (MQ=255)

                                                                                                                               cTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATc                 >  2:4843935/1‑151 (MQ=255)

                                                                                                                                 ggCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATccc               >  1:4455897/1‑151 (MQ=255)

                                                                                                                                     tACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCACATCCCTCTg           >  2:4626486/1‑151 (MQ=255)

                                                                                                                                     tACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTg           <  1:3068417/151‑1 (MQ=255)

                                                                                                                                       ccTCGGCCAGCTAGCCGACAACACGCCCTCCGTGGCCAATATCGAGGATTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCTGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAg         <  2:4492916/151‑1 (MQ=255)

                                                                                                                                        cTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAgg        >  1:2244684/1‑151 (MQ=255)

                                                                                                                                         tCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGt       <  2:8762053/151‑1 (MQ=255)

                                                                                                                                          cGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGtt      >  1:10088688/1‑151 (MQ=255)

                                                                                                                                           ggCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTc     >  2:5653090/1‑151 (MQ=255)

                                                                                                                                             ccAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCag   <  1:8792343/151‑1 (MQ=255)

GGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCTCTGAGGTTCAGG  >  NC_008463/5256231‑5256523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`