Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,530,315 A→G K229K (AAA→AAG)  PA14_RS25330 → iron ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,530,3150AG100.0% 1547.7 / NA 390K229K (AAA→AAG) PA14_RS25330iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (225/165);  total (225/165)

Only 100 of 416 total aligned reads displayed.

TCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGA  >  NC_008463/5530167‑5530461
                                                                                                                                                    |                                                                                                                                                  
tCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTgaagaa                                                                                                                                                  >  2:10213576/1‑151 (MQ=255)
tCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTgaagaa                                                                                                                                                  >  2:453774/1‑151 (MQ=255)
  aaGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaagg                                                                                                                                                >  1:10126795/1‑151 (MQ=255)
  aaGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaagg                                                                                                                                                >  1:3397385/1‑151 (MQ=255)
     ggCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaa                                                                                                                                             <  1:7063241/151‑1 (MQ=255)
       ccAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTCCTACTGGTACACCCTGAagaaggaaaag                                                                                                                                           <  1:6730845/151‑1 (MQ=255)
        cAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAagaaggaaaagg                                                                                                                                          <  1:2021986/151‑1 (MQ=255)
         aggaggAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAggg                                                                                                                                         <  1:9716539/151‑1 (MQ=255)
              gaAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGt                                                                                                                                    >  1:1983745/1‑151 (MQ=255)
                aGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTg                                                                                                                                  <  2:3943461/151‑1 (MQ=255)
                 gCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTgc                                                                                                                                 <  1:353036/151‑2 (MQ=255)
                  ccGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGaa                                                                                                                                <  1:6607767/151‑1 (MQ=255)
                   cGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAAc                                                                                                                               >  2:3374172/1‑151 (MQ=255)
                   cGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGGCACCGCCATGAACGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGACAAGGGCGAGTTGAAc                                                                                                                               >  2:2042811/1‑151 (MQ=255)
                    gAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACt                                                                                                                              <  1:4959648/151‑1 (MQ=255)
                       gATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTCCTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTcgc                                                                                                                           >  2:2464708/1‑151 (MQ=255)
                       gATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTcgc                                                                                                                           >  2:8941364/1‑151 (MQ=255)
                          tGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGACGGAAAAGGGCGAGTTGAACTCGCGcc                                                                                                                        >  2:9535462/1‑151 (MQ=255)
                           ggCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCt                                                                                                                       <  1:5086560/151‑1 (MQ=255)
                               caccccccTGAAGGCCAACCGCTCGAACTACACCCACCACGTCCCCGCCAAGAAAGCCGTGGAGAACGGCGAGGTCGACAAGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCac                                                                                                                   <  2:6873777/145‑1 (MQ=255)
                                     ccTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGACCTCGCGCCTGCACTACTTc                                                                                                             >  1:1449145/1‑151 (MQ=255)
                                     ccTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTc                                                                                                             <  1:1569202/151‑1 (MQ=255)
                                     ccTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTc                                                                                                             >  1:9564236/1‑151 (MQ=255)
                                      cTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACTCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCg                                                                                                            <  1:7252502/151‑1 (MQ=255)
                                        gAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGc                                                                                                          <  1:6290722/151‑1 (MQ=255)
                                        gAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGc                                                                                                          <  1:748425/151‑1 (MQ=255)
                                         aaGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCa                                                                                                         >  2:3737778/1‑151 (MQ=255)
                                         aaGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACAACTTCGGCa                                                                                                         <  2:10441318/151‑1 (MQ=255)
                                          aGGCCTTCGGCTCGATCTACCCCTACAACGTCACCGCCCTGATAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGACGGAAAAGGGCGAGGTGAACTCGCGCCTGCACGACTGCGGCac                                                                                                        >  2:6007483/1‑150 (MQ=255)
                                              ctacGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAg                                                                                                    <  1:8305646/148‑1 (MQ=255)
                                               ttCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAgg                                                                                                   <  1:1610775/151‑1 (MQ=255)
                                                tCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGa                                                                                                  >  1:737761/1‑151 (MQ=255)
                                                  ggCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGAcc                                                                                                >  1:262270/1‑151 (MQ=255)
                                                   gCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGAccc                                                                                               >  1:4438967/1‑151 (MQ=255)
                                                   gCTAGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACCACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGAccc                                                                                               <  2:2236313/151‑1 (MQ=255)
                                                    cTCGATCTACACCAACCACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCg                                                                                              <  1:8448851/151‑1 (MQ=255)
                                                      cGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCggg                                                                                            <  2:8636415/151‑1 (MQ=255)
                                                       gATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgc                                                                                           >  1:130923/1‑151 (MQ=255)
                                                       gATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgc                                                                                           >  2:5548698/1‑151 (MQ=255)
                                                        aTCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcg                                                                                          >  1:5694897/1‑151 (MQ=255)
                                                        aTCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcg                                                                                          >  1:10461159/1‑151 (MQ=255)
                                                         tCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcgc                                                                                         >  2:7111545/1‑151 (MQ=255)
                                                           tACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAATGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGgcgcgc                                                                                       >  1:5266116/1‑151 (MQ=255)
                                                              acCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTgg                                                                                    >  1:8891657/1‑151 (MQ=255)
                                                               ccAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGt                                                                                   >  1:10635485/1‑151 (MQ=255)
                                                                 aacaacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCa                                                                                 >  1:3917358/1‑151 (MQ=255)
                                                                 aacaacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCAGGTCa                                                                                 <  1:2217267/151‑1 (MQ=255)
                                                                   caacGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCAcc                                                                               <  2:7362276/151‑1 (MQ=255)
                                                                      cGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtc                                                                            >  2:6604091/1‑151 (MQ=255)
                                                                       gtaaCCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtct                                                                           <  1:9088931/148‑1 (MQ=255)
                                                                        tCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGtctc                                                                          <  2:1895434/151‑1 (MQ=255)
                                                                            cGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAATGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgc                                                                      >  1:2257132/1‑151 (MQ=255)
                                                                            cGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgc                                                                      <  2:5144916/151‑1 (MQ=255)
                                                                            cGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTGCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGgc                                                                      >  2:4334057/1‑151 (MQ=255)
                                                                               cATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGcgcc                                                                   <  1:4347773/151‑1 (MQ=255)
                                                                                   aaaGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAg                                                               <  1:774001/151‑1 (MQ=255)
                                                                                    aaGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGt                                                              >  1:3632103/1‑151 (MQ=255)
                                                                                          tGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCaa                                                        <  1:5973446/151‑1 (MQ=255)
                                                                                           ggAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAg                                                       <  2:8438728/151‑1 (MQ=255)
                                                                                            gagaACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTCCTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGt                                                      >  1:817551/1‑151 (MQ=255)
                                                                                              gaccGGCGAGGTCGACAAGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTcc                                                    >  2:4475090/4‑151 (MQ=255)
                                                                                              gaACGGCGAGGTCGACATGGCGCTGATCAACCACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTcc                                                    <  2:8750917/151‑1 (MQ=255)
                                                                                                aCGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCag                                                  >  2:7500590/1‑151 (MQ=255)
                                                                                                    cGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcaag                                              <  1:9484699/151‑1 (MQ=255)
                                                                                                     gAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTCGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCagcaagc                                             >  2:10025260/1‑151 (MQ=255)
                                                                                                        gTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAcc                                          >  1:6390091/1‑151 (MQ=255)
                                                                                                          cGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAcccc                                        >  2:6160850/1‑151 (MQ=255)
                                                                                                          cGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCAcccc                                        >  1:7660064/1‑151 (MQ=255)
                                                                                                           gACATGGCGCTGATCAACAACTACGACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCTGCAAGCACCCCa                                       >  1:3770473/1‑151 (MQ=255)
                                                                                                                 gcgcTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAg                                 >  2:3005200/1‑151 (MQ=255)
                                                                                                                    cTGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGccc                              >  1:8044270/1‑151 (MQ=255)
                                                                                                                     tGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCca                             <  2:1717392/151‑1 (MQ=255)
                                                                                                                     tGATCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCca                             <  2:8133236/151‑1 (MQ=255)
                                                                                                                        tCAACAACTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCcagca                          <  2:5022589/151‑1 (MQ=255)
                                                                                                                          aacaacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGt                        <  2:2896470/151‑1 (MQ=255)
                                                                                                                          aacaacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGt                        >  1:7947510/1‑151 (MQ=255)
                                                                                                                           acaacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGtt                       >  1:2877612/1‑151 (MQ=255)
                                                                                                                           acaacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGtt                       >  2:7797868/1‑151 (MQ=255)
                                                                                                                             aacTACTACTGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCACCCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTtcg                     >  1:5852235/1‑151 (MQ=255)
                                                                                                                                 actactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGc                 >  1:4201450/1‑151 (MQ=255)
                                                                                                                                  ctactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGcc                <  2:9730219/151‑1 (MQ=255)
                                                                                                                                  ctactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGcc                >  2:9196835/1‑151 (MQ=255)
                                                                                                                                  ctactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGcc                <  1:10322326/151‑1 (MQ=255)
                                                                                                                                  ctactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGcc                <  2:6474556/151‑1 (MQ=255)
                                                                                                                                  ctactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGcc                <  2:10227157/151‑1 (MQ=255)
                                                                                                                                   tactGGTACACCCTGAAGAAGGAAAAGGTCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGTACCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAATTACAGCAAGCACCCCAGCGAAGCCCAGACGGTCGTCGCCt               >  1:872058/1‑151 (MQ=255)
                                                                                                                                   tactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCt               >  1:4637578/1‑151 (MQ=255)
                                                                                                                                   gactGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCt               <  1:6968642/150‑1 (MQ=255)
                                                                                                                                    actGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCtt              <  1:8626167/151‑1 (MQ=255)
                                                                                                                                    actGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCtt              >  1:4019522/1‑151 (MQ=255)
                                                                                                                                    actGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCtt              <  2:8001693/151‑1 (MQ=255)
                                                                                                                                    actGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGAAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCtt              <  1:1019526/151‑1 (MQ=255)
                                                                                                                                     ctGGTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTc             >  1:3849832/1‑151 (MQ=255)
                                                                                                                                        gTACACCCTGAAGAAGGAAAAGGGCGAGTTGAACTAGAGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATg          <  2:5249745/151‑1 (MQ=255)
                                                                                                                                           cacCCTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTc       <  2:4748964/151‑1 (MQ=255)
                                                                                                                                             cccTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAg     <  1:10001854/151‑1 (MQ=255)
                                                                                                                                             cccTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAg     <  1:3234653/151‑1 (MQ=255)
                                                                                                                                              ccTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGc    <  1:1080642/151‑1 (MQ=255)
                                                                                                                                               cTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCg   <  2:3299688/151‑1 (MQ=255)
                                                                                                                                               cTGAAGAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCGGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCg   <  2:4678393/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCAAGGGCCAGGAGGAAGCCGAGGATTGGCTGACCGGCCTGAAGGCCTTCGGCTCGATCTACACCAACAACGTCACCGCCATGAAAGCCGTGGAGAACGGCGAGGTCGACATGGCGCTGATCAACAACTACTACTGGTACACCCTGAAAAAGGAAAAGGGCGAGTTGAACTCGCGCCTGCACTACTTCGGCAACCAGGACCCGGGCGCGCTGGTCACCGTCTCCGGCGCCGCAGTGCTCAAGTCCAGCAAGCACCCCAGGGAAGCCCAGCAGTTCGTCGCCTTCATGCTCAGCGA  >  NC_008463/5530167‑5530461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑