Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,318,606 A→G E190E (GAA→GAG)  sbrI → ECF family RNA polymerase sigma factor SbrI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,318,6060AG99.8% 1972.0 / NA 505E190E (GAA→GAG) sbrIECF family RNA polymerase sigma factor SbrI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (224/280);  minor base C (1/0);  total (225/280)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 519 total aligned reads displayed.

TGCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAAGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCATG  >  NC_008463/2318458‑2318753
                                                                                                                                                    |                                                                                                                                                   
tGCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAgga                                                                                                                                                   <  1:6335521/151‑1 (MQ=255)
   ccGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGt                                                                                                                                                <  1:5644258/151‑1 (MQ=255)
      aGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGc                                                                                                                                             <  2:4033916/151‑1 (MQ=255)
          aCAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTa                                                                                                                                         <  1:4733315/151‑1 (MQ=255)
           cAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTAt                                                                                                                                        >  1:10177253/1‑151 (MQ=255)
           cAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTAt                                                                                                                                        <  2:6489579/151‑1 (MQ=255)
            aGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATg                                                                                                                                       <  1:1198768/151‑1 (MQ=255)
              cgcgAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGaa                                                                                                                                     <  1:7536494/151‑1 (MQ=255)
               gcgAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAc                                                                                                                                    <  1:89829/151‑1 (MQ=255)
                cgAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAcc                                                                                                                                   >  2:1057937/1‑151 (MQ=255)
                cgAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAcc                                                                                                                                   >  2:4439506/1‑151 (MQ=255)
                cgAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAcc                                                                                                                                   <  1:551930/151‑1 (MQ=255)
                 gAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAccc                                                                                                                                  >  1:7984217/1‑151 (MQ=255)
                 gAGGTATACCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGAGCAGAAGCAGCGCCGCCAGCTCGCCGAGGAGGTGGCCGGAAGAAccc                                                                                                                                  >  2:9758820/1‑151 (MQ=255)
                       ttCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGaa                                                                                                                            >  2:5503755/1‑151 (MQ=255)
                           tGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTTTGAACCCACAGAAGCAt                                                                                                                        >  1:2732845/1‑151 (MQ=255)
                            gATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCAtt                                                                                                                       >  1:310295/1‑151 (MQ=255)
                              tGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTcc                                                                                                                     <  1:10485306/151‑1 (MQ=255)
                              tGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTcc                                                                                                                     <  2:3716232/151‑1 (MQ=255)
                              tGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTcc                                                                                                                     <  1:8854735/151‑1 (MQ=255)
                              tGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTcc                                                                                                                     >  2:5224120/1‑151 (MQ=255)
                                cgcgcCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCcgc                                                                                                                   <  2:2376737/151‑1 (MQ=255)
                                   gcCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCTGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAg                                                                                                                <  1:1931627/151‑1 (MQ=255)
                                   gcCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAg                                                                                                                <  1:2249859/151‑1 (MQ=255)
                                    cccACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGc                                                                                                               <  2:6155629/151‑1 (MQ=255)
                                       aCGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGTGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTc                                                                                                            <  2:3514684/151‑1 (MQ=255)
                                        cGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCg                                                                                                           >  1:824042/1‑151 (MQ=255)
                                         ggAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGc                                                                                                          >  2:1435589/1‑151 (MQ=255)
                                          gagaACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGcc                                                                                                         <  1:6756486/151‑1 (MQ=255)
                                             aactggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCTGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGaa                                                                                                      >  1:539203/1‑151 (MQ=255)
                                               ctggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAaga                                                                                                    <  1:6016493/151‑1 (MQ=255)
                                                 ggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGc                                                                                                  <  1:2080386/151‑1 (MQ=255)
                                                  gaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCa                                                                                                 <  2:6652353/151‑1 (MQ=255)
                                                  gaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCa                                                                                                 <  1:1555705/151‑1 (MQ=255)
                                                    actggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGa                                                                                               <  2:3006025/151‑1 (MQ=255)
                                                        gCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCt                                                                                           >  2:893986/1‑151 (MQ=255)
                                                         ccGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTg                                                                                          <  2:1320962/151‑1 (MQ=255)
                                                          cGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTgg                                                                                         <  2:1090384/151‑1 (MQ=255)
                                                            agaTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGgcg                                                                                       >  1:3329228/1‑151 (MQ=255)
                                                             gaTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGCGGAGGTGGCGGGTTGAACCCCCAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGgcgc                                                                                      >  1:3918465/1‑151 (MQ=255)
                                                             gaTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGGGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGgcgc                                                                                      >  2:2563183/1‑151 (MQ=255)
                                                              aTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCa                                                                                     <  1:6580972/151‑1 (MQ=255)
                                                                  ccACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTc                                                                                 <  1:425916/151‑1 (MQ=255)
                                                                   cACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTcc                                                                                >  2:1737563/1‑151 (MQ=255)
                                                                   cACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTcc                                                                                <  1:5129576/151‑1 (MQ=255)
                                                                    aCGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCcg                                                                               <  1:7413260/151‑1 (MQ=255)
                                                                       cTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCgcgc                                                                            <  1:1763826/151‑1 (MQ=255)
                                                                          aCCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCa                                                                         <  1:3764984/151‑1 (MQ=255)
                                                                            ccAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCGACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCAGTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCAcg                                                                       <  2:5335483/151‑1 (MQ=255)
                                                                              aGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCAcgcg                                                                     >  2:6961528/1‑151 (MQ=255)
                                                                               gTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACgcgc                                                                    <  2:3136833/151‑1 (MQ=255)
                                                                               gTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACgcgc                                                                    <  2:9292317/151‑1 (MQ=255)
                                                                                   ccgcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCCGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGGAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgca                                                                >  1:4956681/1‑151 (MQ=255)
                                                                                     gcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcagc                                                              <  2:3315060/151‑1 (MQ=255)
                                                                                     gcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcagc                                                              <  1:7815651/151‑1 (MQ=255)
                                                                                     gcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcagc                                                              <  2:10523240/151‑1 (MQ=255)
                                                                                     gcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCAGTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcagc                                                              <  2:8086412/151‑1 (MQ=255)
                                                                                     gcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGGATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcagc                                                              >  2:8442691/1‑151 (MQ=255)
                                                                                      cTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcagca                                                             <  2:6845769/151‑1 (MQ=255)
                                                                                        ggAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCagcagc                                                           <  1:8568392/151‑1 (MQ=255)
                                                                                         gagaCGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATTCTGGCGCATTTACGCGCCCACGCGCCGCAGCAgcc                                                          >  1:9344728/1‑151 (MQ=255)
                                                                                          agaCGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccg                                                         <  1:9614469/151‑1 (MQ=255)
                                                                                             cGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcg                                                      >  1:1602484/1‑151 (MQ=255)
                                                                                                tGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTACGCGCCCACGCGCCGCAGCAgccggcgccg                                                   >  1:6141080/1‑151 (MQ=255)
                                                                                                  aaGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcgccggc                                                 <  1:2205983/151‑1 (MQ=255)
                                                                                                  aaGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcgccggc                                                 >  1:4374188/1‑151 (MQ=255)
                                                                                                  aaGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcgccggc                                                 <  1:5978221/151‑1 (MQ=255)
                                                                                                   agagCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTTGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATTCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGccggcgccggcg                                                >  2:560244/1‑151 (MQ=255)
                                                                                                   agagCCGTTTCCGCTACGCGGTGCAGAAACTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCCTTCCGCGAGCGTCGCCGAAGCGCCGAAGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCCGccggcgccggcg                                                >  2:2293764/1‑151 (MQ=255)
                                                                                                    gagCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCcggcgccggcgc                                               >  2:6283366/1‑151 (MQ=255)
                                                                                                      gCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCCCAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTg                                             <  1:9769528/151‑1 (MQ=255)
                                                                                                        cGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGa                                           >  1:9940601/1‑151 (MQ=255)
                                                                                                         gTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGAc                                          >  1:10012442/1‑151 (MQ=255)
                                                                                                            tCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAg                                       <  1:3447050/151‑1 (MQ=255)
                                                                                                               gCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCg                                    >  2:4664620/1‑151 (MQ=255)
                                                                                                                  aCGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATc                                 >  1:7010994/1‑151 (MQ=255)
                                                                                                                     cgGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCAGTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTc                              <  2:4871180/151‑1 (MQ=255)
                                                                                                                      ggTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcg                             <  1:7182901/151‑1 (MQ=255)
                                                                                                                       gTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgc                            >  2:7522697/1‑151 (MQ=255)
                                                                                                                        tGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgcc                           <  1:9192345/151‑1 (MQ=255)
                                                                                                                        tGCAGAAGCTGCGCCGGCTGCGGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgcc                           <  2:10110972/151‑1 (MQ=255)
                                                                                                                         gCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTACCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgccg                          <  1:6285720/151‑1 (MQ=255)
                                                                                                                           aGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgccgcc                        <  2:2909319/151‑1 (MQ=255)
                                                                                                                           aGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgccgcc                        >  2:7214666/1‑151 (MQ=255)
                                                                                                                           aGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgccgcc                        >  2:3838402/1‑151 (MQ=255)
                                                                                                                             aaGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgccgccgc                      <  2:6218999/151‑1 (MQ=255)
                                                                                                                              aGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCcgcg                     <  1:25347/151‑1 (MQ=255)
                                                                                                                                      cGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGACGCAGTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCa             <  1:5234716/151‑1 (MQ=255)
                                                                                                                                        gctgctGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAgg           >  1:10355328/1‑151 (MQ=255)
                                                                                                                                         ctgctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAggc          <  1:5048259/151‑1 (MQ=255)
                                                                                                                                         ctgctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAggc          <  2:7668724/151‑1 (MQ=255)
                                                                                                                                          tgctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAggcg         <  2:9141755/151‑1 (MQ=255)
                                                                                                                                          tgctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAggcg         >  2:2150227/1‑151 (MQ=255)
                                                                                                                                            ctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAggcggc       <  2:5866728/151‑1 (MQ=255)
                                                                                                                                            ctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAggcggc       <  2:2781936/151‑1 (MQ=255)
                                                                                                                                              ggCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGgcgc     >  2:7881830/1‑151 (MQ=255)
                                                                                                                                               gCCGAGGAGGTGGCGGTATGACCCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCa    <  1:10644242/151‑1 (MQ=255)
                                                                                                                                                ccGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCAt   >  1:1456819/1‑151 (MQ=255)
                                                                                                                                                ccGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCAt   >  2:8629497/1‑151 (MQ=255)
                                                                                                                                                ccGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCAt   >  1:10096776/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAAGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCATG  >  NC_008463/2318458‑2318753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑