Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,545,609	T→C	I509I (ATT→ATC)	tssI →	type VI secretion system tip protein TssI/VgrG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,545,609	0	T	C	100.0%	196.4 / NA	62	I509I (ATT→ATC)	tssI	type VI secretion system tip protein TssI/VgrG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/36);  total (26/36)

CCTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACG  >  NC_008463/2545461‑2545756                                                                                                                                                     |                                                                                                                                                    ccTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                   >  1:2040303/1‑151 (MQ=14) ccTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                   <  1:3347051/151‑1 (MQ=14) ccTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGa                                                                                                                                                   <  1:4461974/151‑1 (MQ=14)  cTACGATCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCAGGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                  <  2:2848463/151‑1 (MQ=9)  cTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAg                                                                                                                                                  >  2:7678098/1‑151 (MQ=14)   tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                 <  1:9392299/151‑1 (MQ=14)   tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                 >  2:5638246/1‑151 (MQ=14)   tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                 >  2:7647161/1‑151 (MQ=14)   tACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAgg                                                                                                                                                 <  1:450713/151‑1 (MQ=14)    aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                                >  1:5130574/1‑151 (MQ=14)    aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                                >  2:2444980/1‑151 (MQ=14)    aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                                >  2:2445005/1‑151 (MQ=14)    aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                                >  1:3847414/1‑151 (MQ=14)    aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                                >  2:7994960/1‑151 (MQ=14)    aCGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGt                                                                                                                                                <  2:8922878/151‑1 (MQ=14)     cGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtc                                                                                                                                               <  2:7068992/151‑2 (MQ=14)      gAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                              <  2:1270594/151‑3 (MQ=11)      gAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGtcg                                                                                                                                              >  1:9079155/1‑149 (MQ=11)                                                                                                                                  ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                  >  2:692078/1‑151 (MQ=255)                                                                                                                                  ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                  <  2:1762099/151‑1 (MQ=255)                                                                                                                                  ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                  <  2:5977112/151‑1 (MQ=255)                                                                                                                                  ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                  <  1:6889292/151‑1 (MQ=255)                                                                                                                                   cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                 <  2:5397233/151‑1 (MQ=255)                                                                                                                                   cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                 <  2:1539676/151‑1 (MQ=255)                                                                                                                                   cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGca                 >  1:3253081/1‑150 (MQ=11)                                                                                                                                    gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa                >  2:3686773/1‑151 (MQ=255)                                                                                                                                    gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa                >  2:8657559/1‑151 (MQ=255)                                                                                                                                     agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa               <  1:5156624/151‑1 (MQ=255)                                                                                                                                     agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa               >  2:6917274/1‑151 (MQ=255)                                                                                                                                     agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGAACGTGGACAACTACGAGACCATCACCGTGCATGCCaa               <  2:9770788/151‑1 (MQ=11)                                                                                                                                      gagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc              <  1:1558571/151‑1 (MQ=255)                                                                                                                                      gagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc              <  1:4807806/151‑1 (MQ=255)                                                                                                                                       agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc             <  1:1942608/151‑1 (MQ=255)                                                                                                                                       agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc             <  1:2427230/151‑1 (MQ=255)                                                                                                                                       agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc             <  2:5151483/151‑1 (MQ=255)                                                                                                                                        ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCg            <  1:2448541/151‑1 (MQ=255)                                                                                                                                        ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCg            <  1:7331022/151‑1 (MQ=255)                                                                                                                                          aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca          <  1:1173533/151‑1 (MQ=255)                                                                                                                                          aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca          <  2:7870635/151‑1 (MQ=255)                                                                                                                                          aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca          >  1:5867505/1‑151 (MQ=255)                                                                                                                                          aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca          <  2:5556596/151‑1 (MQ=255)                                                                                                                                          aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca          <  2:4976197/151‑1 (MQ=255)                                                                                                                                           aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag         <  2:10059541/151‑1 (MQ=255)                                                                                                                                           aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag         <  2:4634964/151‑1 (MQ=255)                                                                                                                                            ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACCACAACGAGACCATCACCGAGCATGCCAACCgcagc        >  2:5523216/1‑151 (MQ=11)                                                                                                                                            ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc        <  2:155001/151‑1 (MQ=255)                                                                                                                                            ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc        >  1:4991711/1‑151 (MQ=255)                                                                                                                                            ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc        >  2:6244849/1‑151 (MQ=255)                                                                                                                                            ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc        >  1:6657829/1‑151 (MQ=255)                                                                                                                                             cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca       <  1:7146615/151‑1 (MQ=255)                                                                                                                                              aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa      <  2:3710641/151‑1 (MQ=255)                                                                                                                                              aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa      >  1:8182734/1‑151 (MQ=255)                                                                                                                                              aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa      >  2:8320294/1‑151 (MQ=255)                                                                                                                                              aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa      <  2:9515237/151‑1 (MQ=255)                                                                                                                                               ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg     >  1:6165970/1‑151 (MQ=255)                                                                                                                                               ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg     >  1:8371231/1‑151 (MQ=255)                                                                                                                                                gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa    >  2:4245580/1‑151 (MQ=255)                                                                                                                                                gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa    >  1:9058840/1‑151 (MQ=255)                                                                                                                                                 aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc   <  1:3898035/151‑1 (MQ=255)                                                                                                                                                 aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc   <  2:9490749/151‑1 (MQ=255)                                                                                                                                                 aCATCGAGGTCGAGAACGACGAGAGCCACTGGGACGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc   <  2:9490574/151‑1 (MQ=14)                                                                                                                                                  cATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACg  <  1:9729208/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CCTACGAGCTGCCGGCGAACGCTACCCAGAGCGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACG  >  NC_008463/2545461‑2545756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑