Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 888,038 2 bp→TG coding (1562‑1563/1878 nt) PA14_RS04165 → sigma‑54‑dependent Fis family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463888,0380CT99.0% 2175.4 / NA 515P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (270/240);  minor base G (3/2);  total (273/242)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463888,0390TG99.8% 2002.7 / ‑4.3 515P521M (CCT→CTG) PA14_RS04165sigma‑54‑dependent Fis family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (273/240);  minor base A (0/1);  total (273/242)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 515 total aligned reads displayed.

GAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGCC  >  NC_008463/887893‑888183
                                                                                                                                                 ||                                                                                                                                                
gAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAAt                                                                                                                                              >  2:8911445/1‑151 (MQ=255)
gAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAAt                                                                                                                                              <  1:9547299/151‑1 (MQ=255)
   gATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGcc                                                                                                                                           >  2:8525635/1‑151 (MQ=255)
       tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCt                                                                                                                                       <  1:7370131/151‑1 (MQ=255)
       tctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCt                                                                                                                                       >  1:290993/1‑151 (MQ=255)
        ctACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTc                                                                                                                                      <  2:4203625/151‑1 (MQ=255)
          aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                    >  1:10418931/1‑151 (MQ=255)
          aCTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCgg                                                                                                                                    <  1:6799489/151‑1 (MQ=255)
           cTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGc                                                                                                                                   >  2:7726242/1‑151 (MQ=255)
            ttCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCt                                                                                                                                  >  2:4859864/1‑151 (MQ=255)
              ccGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTAt                                                                                                                                <  1:4465853/151‑1 (MQ=255)
               cGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATc                                                                                                                               <  2:9714596/151‑1 (MQ=255)
               cGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATc                                                                                                                               >  2:906207/1‑151 (MQ=255)
                gCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCg                                                                                                                              >  2:9479564/1‑151 (MQ=255)
                gCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCg                                                                                                                              <  2:6972247/151‑1 (MQ=255)
                 ccTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGc                                                                                                                             >  1:4362481/1‑151 (MQ=255)
                  cTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCt                                                                                                                            >  1:5234059/1‑151 (MQ=255)
                     ggcggcGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGc                                                                                                                         >  1:1388648/1‑151 (MQ=255)
                      gcggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGcc                                                                                                                        <  2:2148666/151‑1 (MQ=255)
                       cggcgCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCg                                                                                                                       >  2:10416048/1‑151 (MQ=255)
                          cgcCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGc                                                                                                                    <  1:5707938/151‑1 (MQ=255)
                           gcCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCa                                                                                                                   >  1:5684826/1‑151 (MQ=255)
                             ccGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAAt                                                                                                                 <  1:3859887/151‑1 (MQ=255)
                             ccGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAAt                                                                                                                 >  2:4761488/1‑151 (MQ=255)
                               gCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGt                                                                                                               <  1:1567432/151‑1 (MQ=255)
                               gCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGt                                                                                                               >  2:1760198/1‑151 (MQ=255)
                                 ttCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTcc                                                                                                             >  2:10234487/1‑151 (MQ=255)
                                   cGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGc                                                                                                           >  1:10069679/1‑151 (MQ=255)
                                   cGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGc                                                                                                           >  2:8075080/1‑151 (MQ=255)
                                    gAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGcc                                                                                                          >  1:3214105/1‑151 (MQ=255)
                                     aaCTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCa                                                                                                         <  2:18376/151‑1 (MQ=255)
                                      aCTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCACTGTCCGCCAg                                                                                                        >  2:7121752/1‑151 (MQ=255)
                                      aCTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAg                                                                                                        >  1:7795340/1‑151 (MQ=255)
                                       cTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGt                                                                                                       <  1:8726757/151‑1 (MQ=255)
                                        tGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGtt                                                                                                      <  2:7466585/151‑1 (MQ=255)
                                         gccgccGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTg                                                                                                     <  1:1072048/151‑1 (MQ=255)
                                          ccgccgCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGc                                                                                                    <  2:6871991/151‑1 (MQ=255)
                                          ccgccgCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGc                                                                                                    <  1:7925999/151‑1 (MQ=255)
                                           cgccgcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCg                                                                                                   <  2:3486362/151‑1 (MQ=255)
                                               gcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAt                                                                                               <  1:9442705/151‑1 (MQ=255)
                                               gcTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAt                                                                                               >  2:10036535/1‑151 (MQ=255)
                                                 tACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtgt                                                                                             <  1:8505469/151‑1 (MQ=255)
                                                 tACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtgt                                                                                             <  1:4881135/151‑1 (MQ=255)
                                                  aCGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCAtgtg                                                                                            >  2:7836690/1‑151 (MQ=255)
                                                     cgAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTg                                                                                         <  2:2558859/151‑1 (MQ=255)
                                                         cGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCt                                                                                     >  1:2741863/1‑151 (MQ=255)
                                                          gCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGTGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGAGGCATGTGTTGCGCTa                                                                                    >  1:5093829/1‑151 (MQ=255)
                                                          gCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTa                                                                                    <  1:7242071/151‑1 (MQ=255)
                                                          gCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTa                                                                                    <  1:4530637/151‑1 (MQ=255)
                                                           cAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTAc                                                                                   >  1:761540/1‑151 (MQ=255)
                                                            aGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACg                                                                                  >  1:358821/1‑151 (MQ=255)
                                                                 ccGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgc                                                                             <  2:5576423/151‑1 (MQ=255)
                                                                 ccGGCGGGCGCTGACCCCGCGCATCCTCGACGCGGAAACCGCGCACCGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGACTGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTGGCGGCATGTGTTGCGCGACGCCTgc                                                                             <  2:8360439/151‑1 (MQ=255)
                                                                   ggCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTgcgc                                                                           >  1:2455355/1‑151 (MQ=255)
                                                                      tGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGTGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                        >  1:6317858/1‑151 (MQ=255)
                                                                      tGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCt                                                                        <  1:2137254/151‑1 (MQ=255)
                                                                         cgcTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCtgtg                                                                     >  1:5538730/1‑151 (MQ=255)
                                                                         cgcTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCtgtg                                                                     <  2:1278193/151‑1 (MQ=255)
                                                                            tGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcgg                                                                  >  2:5925406/1‑151 (MQ=255)
                                                                              aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                                >  2:660492/1‑151 (MQ=255)
                                                                              aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                                <  2:6415543/151‑1 (MQ=255)
                                                                              aTCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcg                                                                <  2:10666518/151‑1 (MQ=255)
                                                                                ccGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTgcggcggc                                                              <  2:10303087/151‑1 (MQ=255)
                                                                                  ggCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGgcgc                                                            >  2:666631/1‑151 (MQ=255)
                                                                                   gcgcATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGcc                                                           <  2:4461227/151‑1 (MQ=255)
                                                                                           tCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCa                                                   <  1:2323884/151‑1 (MQ=255)
                                                                                            cgacgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCaa                                                  <  1:3472857/151‑1 (MQ=255)
                                                                                               cgaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTc                                               <  2:6119337/151‑1 (MQ=255)
                                                                                                gaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGTAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCTACTCg                                              >  1:10582601/1‑151 (MQ=255)
                                                                                                gaGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCg                                              >  2:5029113/1‑151 (MQ=255)
                                                                                                    aaaCCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGa                                          >  2:7369363/1‑151 (MQ=255)
                                                                                                    aaaCCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGa                                          >  2:6208239/1‑151 (MQ=255)
                                                                                                    aaaCCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGa                                          >  2:6772830/1‑151 (MQ=255)
                                                                                                        cgcgCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCtt                                      >  1:9165608/1‑151 (MQ=255)
                                                                                                            cACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCgg                                  >  2:2936054/1‑151 (MQ=255)
                                                                                                             acagcggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGc                                 <  1:8857001/148‑1 (MQ=255)
                                                                                                             aCTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCCGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGc                                 >  2:2255543/1‑151 (MQ=255)
                                                                                                             aCTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGc                                 >  1:4513384/1‑151 (MQ=255)
                                                                                                                 cggcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCagag                             <  2:5200317/151‑1 (MQ=255)
                                                                                                                   gcgTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCt                           <  2:8707869/151‑1 (MQ=255)
                                                                                                                     gTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGc                         >  1:3317186/1‑151 (MQ=255)
                                                                                                                     gTACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGGGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGc                         >  1:9720558/1‑151 (MQ=255)
                                                                                                                      tACGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCg                        <  1:9286744/151‑1 (MQ=255)
                                                                                                                        cGCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTg                      <  1:5880955/151‑1 (MQ=255)
                                                                                                                         gCATCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTgg                     >  2:3126062/1‑151 (MQ=255)
                                                                                                                           aTCGAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCg                   >  2:8596635/1‑151 (MQ=255)
                                                                                                                              gAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAg                >  1:1907629/1‑151 (MQ=255)
                                                                                                                              gAACTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAg                <  1:9880469/151‑1 (MQ=255)
                                                                                                                               aaCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAgg               >  1:8159642/1‑151 (MQ=255)
                                                                                                                                aCTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGc              <  2:9281693/151‑1 (MQ=255)
                                                                                                                                 cTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGcc             >  1:673729/1‑151 (MQ=255)
                                                                                                                                 cTCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCGATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGcc             <  2:4728969/151‑1 (MQ=255)
                                                                                                                                  tCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCg            <  2:3249519/151‑1 (MQ=255)
                                                                                                                                  tCGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCg            >  2:9175650/1‑151 (MQ=255)
                                                                                                                                   cGGCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCgg           >  1:3077433/1‑151 (MQ=255)
                                                                                                                                    ggCGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGa          >  1:1243828/1‑151 (MQ=255)
                                                                                                                                      cGAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGaca        >  1:8396822/1‑151 (MQ=255)
                                                                                                                                       gAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGacac       <  2:3684206/151‑1 (MQ=255)
                                                                                                                                       gAGGCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGacac       >  1:5334134/1‑151 (MQ=255)
                                                                                                                                        cggCAGCCCTGGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACAcc      <  2:3806581/150‑1 (MQ=255)
                                                                                                                                                 ||                                                                                                                                                
GAGGATCTCTACTTCCGCCTCGGCGGCGCCCGCTTCGAACTGCCGCCGCTACGCGAACGCAGCGACCGGCTGGCGCTGATCCGGCGCATCCTCGACGAGGAAACCGCGCACTGCGGCGTACGCATCGAACTCGGCGAGGCAGCCCCTGAATGCCTGCTCGGCTATCGCTGGCCGGGCAATGTCCGCCAGTTGCGGCATGTGTTGCGCTACGCCTGCGCGCTGTGCGGCGGCGCCACCCTGCAACTCGCCGACCTTCCGGCAGAGCTGCGTGGCGAAGGCCGGACACCGGCC  >  NC_008463/887893‑888183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑