Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,545,633 2 bp→CT coding (1551‑1552/2070 nt) tssI → type VI secretion system tip protein TssI/VgrG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,545,6330TC99.2% 447.3 / ‑6.4 120H517H (CAT→CAC) tssItype VI secretion system tip protein TssI/VgrG
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (36/83);  total (36/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0084632,545,6340CT99.2% 478.4 / ‑4.5 120R518W (CGG→TGG) tssItype VI secretion system tip protein TssI/VgrG
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (36/83);  total (36/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 120 total aligned reads displayed.

CCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGG‑TCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCA  >  NC_008463/2545590‑2545778
                                           ||                                                                                                                                                 
ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                                        <  1:6889292/151‑1 (MQ=255)
ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                                        >  2:692078/1‑151 (MQ=255)
ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                                        <  2:1762099/151‑1 (MQ=255)
ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                                        <  2:5977112/151‑1 (MQ=255)
 cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                                       <  2:1539676/151‑1 (MQ=255)
 cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                                       <  2:5397233/151‑1 (MQ=255)
 cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGca                                       >  1:3253081/1‑150 (MQ=11)
  gagaGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCa                                      >  2:3686773/1‑151 (MQ=255)
   agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa                                     >  2:6917274/1‑151 (MQ=255)
   agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa                                     <  1:5156624/151‑1 (MQ=255)
   agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGAACGTGGACAACTACGAGACCATCACCGTGCATGCCaa                                     <  2:9770788/151‑1 (MQ=11)
    gagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc                                    <  1:4807806/151‑1 (MQ=255)
     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc                                   <  1:2427230/151‑1 (MQ=255)
     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc                                   <  1:1942608/151‑1 (MQ=255)
     agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc                                   <  2:5151483/151‑1 (MQ=255)
      ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCg                                  <  1:2448541/151‑1 (MQ=255)
      ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCg                                  <  1:7331022/151‑1 (MQ=255)
        aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca                                <  2:5556596/151‑1 (MQ=255)
        aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca                                <  1:1173533/151‑1 (MQ=255)
        aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca                                <  2:4976197/151‑1 (MQ=255)
        aaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgca                                <  2:7870635/151‑1 (MQ=255)
         aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag                               <  2:10059541/151‑1 (MQ=255)
         aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag                               <  2:4634964/151‑1 (MQ=255)
          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACCACAACGAGACCATCACCGAGCATGCCAACCgcagc                              >  2:5523216/1‑151 (MQ=11)
          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc                              >  1:6657829/1‑151 (MQ=255)
          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc                              >  1:4991711/1‑151 (MQ=255)
          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc                              >  2:6244849/1‑151 (MQ=255)
          ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc                              <  2:155001/151‑1 (MQ=255)
           cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca                             <  1:7146615/151‑1 (MQ=255)
            aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa                            <  2:9515237/151‑1 (MQ=255)
            aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa                            >  1:8182734/1‑151 (MQ=255)
            aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa                            >  2:8320294/1‑151 (MQ=255)
             ggACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg                           >  1:8371231/1‑151 (MQ=255)
             ggACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg                           >  1:6165970/1‑151 (MQ=255)
              gACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa                          >  2:4245580/1‑151 (MQ=255)
              gACATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGa                          >  1:9058840/1‑151 (MQ=255)
               aCATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc                         <  2:9490749/151‑1 (MQ=255)
               aCATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc                         <  1:3898035/151‑1 (MQ=255)
               aCATCGAGGTCGAGAACGACGAGAGCCACTGGG‑ACGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc                         <  2:9490574/151‑1 (MQ=14)
                cATCGAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACg                        <  1:9729208/151‑1 (MQ=255)
                  tcgcggTCGAGCACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGCTCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGt                      <  2:3484237/147‑1 (MQ=14)
                  tcgAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGt                      <  2:6116890/149‑1 (MQ=255)
                   cgAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTc                     >  1:7559060/2‑151 (MQ=255)
                   cgAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTc                     <  1:1406363/150‑1 (MQ=255)
                    gAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCCTCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCg                    <  2:7704776/151‑1 (MQ=255)
                    gAGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCg                    <  2:923230/151‑1 (MQ=255)
                     aGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGa                   <  1:5608089/151‑1 (MQ=255)
                     aGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGa                   <  2:9120093/151‑1 (MQ=255)
                     aGGTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGa                   <  2:9120128/151‑1 (MQ=255)
                      ggTCGAGAACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAc                  <  1:7593308/151‑1 (MQ=255)
                       gtcgagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAcc                 <  2:4611565/148‑1 (MQ=255)
                       gtcgagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAcc                 <  2:5011910/148‑1 (MQ=255)
                       gtcgagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAcc                 <  1:7732194/148‑1 (MQ=255)
                       gtcgagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAcc                 >  1:8948161/4‑151 (MQ=255)
                       gtcgagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAcc                 <  2:8686819/148‑1 (MQ=255)
                       gtcgagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAcc                 <  2:8693031/148‑1 (MQ=255)
                       gtcgagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAcc                 <  1:9853959/148‑1 (MQ=255)
                        tcgagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCg                <  2:485059/149‑1 (MQ=255)
                        tcgagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCg                <  2:484998/149‑1 (MQ=255)
                        gcgagcACGACGAGAGCCACTGGG‑CCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGCAGCACGATCGCACCGAGACCGTGGACCACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCg                <  2:9047705/149‑1 (MQ=17)
                          gagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCa              >  2:9213751/1‑151 (MQ=255)
                          gagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCa              <  2:7293437/151‑1 (MQ=255)
                          gagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCa              <  1:5182807/151‑1 (MQ=255)
                          gagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCa              >  2:1630611/1‑151 (MQ=255)
                          gagaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCa              <  2:5665728/151‑1 (MQ=255)
                           agaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCaa             >  2:3385661/1‑151 (MQ=255)
                            gaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAAc            <  1:6105402/151‑1 (MQ=255)
                            gaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAAc            <  1:9170106/151‑1 (MQ=255)
                            gaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAAc            <  1:9170297/151‑1 (MQ=255)
                            gaACGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAAc            <  2:6086141/151‑1 (MQ=255)
                            gaACGACGAGAGCCACTGGA‑TCGGCCACGACCGGACCAAGACCTTCGTCCACGACGAGTCGGTGCATGTGAAGCACGATCGCACCGAGTCCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCTAc            <  1:6138511/151‑1 (MQ=17)
                             aaCGACGAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACg           <  2:10657387/151‑1 (MQ=255)
                              acgacgAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACga          >  1:2823160/1‑151 (MQ=255)
                              acgacgAGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACga          >  2:10460382/1‑151 (MQ=255)
                               cgacgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgag         >  2:9576556/1‑151 (MQ=255)
                               cgacgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgag         <  1:203817/151‑1 (MQ=255)
                               cgacgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgag         <  2:8068100/151‑1 (MQ=255)
                                gacgaGAGCCACTGGG‑TCGGCCCCGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgaga        >  2:2111239/1‑151 (MQ=255)
                                gacgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgaga        >  1:7913006/1‑151 (MQ=255)
                                gacgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgaga        >  2:10406546/1‑151 (MQ=255)
                                gacgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgaga        <  1:1277981/151‑1 (MQ=255)
                                 acgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGAc       >  2:8671943/1‑151 (MQ=255)
                                 acgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGAc       <  1:10598271/151‑1 (MQ=255)
                                 acgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGAc       <  2:3751192/151‑1 (MQ=255)
                                 acgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGAc       >  1:410709/1‑151 (MQ=255)
                                 acgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGAc       <  1:1524700/151‑1 (MQ=255)
                                  cgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACg      >  1:9222904/1‑151 (MQ=255)
                                  cgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACg      <  1:7964173/151‑1 (MQ=255)
                                  cgaGAGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACg      <  2:6555096/151‑1 (MQ=255)
                                  cgaGAGCC‑TCGGGCTCGGCCACGACCGGACAAAGACCATCGACCACGACGAGACGGTTCATGTTTAGCTCGATAGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACg      <  2:5807202/151‑1 (MQ=18)
                                   gagaGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACgg     <  1:4018786/151‑1 (MQ=255)
                                   gagaGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACgg     <  2:4870345/151‑1 (MQ=255)
                                   gagaGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACgg     >  2:9142448/1‑151 (MQ=255)
                                   gagaGCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACgg     >  1:7446135/1‑151 (MQ=255)
                                     gagCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTc   <  2:4238089/151‑1 (MQ=255)
                                      agCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCa  <  2:7999400/151‑1 (MQ=255)
                                      agCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCa  <  2:8989438/151‑1 (MQ=255)
                                      agCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCa  <  1:4188882/151‑1 (MQ=255)
                                      agCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCa  <  2:7037748/151‑1 (MQ=255)
                                      agCCACTGGG‑TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCa  <  2:3590500/151‑1 (MQ=255)
                                           ||                                                                                                                                                 
CCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGG‑TCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCA  >  NC_008463/2545590‑2545778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑