BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,198,146	Δ1 bp	coding (230/1980 nt)	PA14_RS14610 ←	cation/acetate symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,198,144	0	G	.	99.5%	2001.8 / 0.9	410	coding (232/1980 nt)	PA14_RS14610	cation/acetate symporter
Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (197/209); minor base C (2/0); total (199/211)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

CGGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGC  >  NC_008463/3197994‑3198289

cgGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAg                                                                                                                                                   <  2:7373943/151‑1 (MQ=255)

 gggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAgg                                                                                                                                                  <  1:1943498/151‑1 (MQ=255)

  ggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggc                                                                                                                                                 <  1:2953436/151‑2 (MQ=255)

  ggCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggc                                                                                                                                                 <  1:515721/151‑2 (MQ=255)

     gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                              >  1:6658436/1‑147 (MQ=255)

     gAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAggcaca                                                                                                                                              <  2:10194393/151‑5 (MQ=255)

      aGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACag                                                                                                                                            >  1:4809201/1‑151 (MQ=255)

      aGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACag                                                                                                                                            <  1:4635845/151‑1 (MQ=255)

       ggCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACaga                                                                                                                                           <  1:8893758/151‑1 (MQ=255)

          cGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACagaaga                                                                                                                                        <  1:9356950/151‑1 (MQ=255)

           gACAGCAGGCCTGAGTGCAGCGACAACGCCAGGGGCAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAgaagaa                                                                                                                                       <  2:6091505/151‑1 (MQ=255)

           gACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAgaagaa                                                                                                                                       >  2:513826/1‑151 (MQ=255)

             cagcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAaca                                                                                                                                     >  2:4423447/1‑151 (MQ=255)

             cagcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAaca                                                                                                                                     <  2:151299/151‑1 (MQ=255)

              agcagGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAacac                                                                                                                                    <  1:2443802/151‑1 (MQ=255)

                 agGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGaa                                                                                                                                 <  2:2086472/151‑1 (MQ=255)

                       gAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGaa                                                                                                                           <  2:5181440/151‑1 (MQ=255)

                       gAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGaa                                                                                                                           <  1:8082306/151‑1 (MQ=255)

                        aGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAac                                                                                                                          <  1:5780444/151‑1 (MQ=255)

                         gTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAaca                                                                                                                         <  2:5976341/151‑1 (MQ=255)

                          tGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAacac                                                                                                                        <  2:8630472/151‑1 (MQ=255)

                            cAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgc                                                                                                                      <  1:6423927/151‑1 (MQ=255)

                                 aCAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACgccggcc                                                                                                                 >  1:2296723/1‑151 (MQ=255)

                                  cAACGCCACTGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGTCCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGCGCGCCGACAA‑GGCACCTAAGAACACGAACAGGAACACgccggcct                                                                                                                <  2:177867/151‑2 (MQ=255)

                                    aCGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGATCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GTCACAGAAGAACACGAACAGGAACACGCCGGCcgcg                                                                                                              >  2:11122287/1‑151 (MQ=255)

                                     cGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGa                                                                                                             >  2:9641856/1‑151 (MQ=255)

                                         aGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAcccc                                                                                                         >  2:3572131/1‑151 (MQ=255)

                                         aGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAcccc                                                                                                         <  2:8452629/151‑1 (MQ=255)

                                         aGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGAcccc                                                                                                         >  2:10868977/1‑151 (MQ=255)

                                            ggAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGaa                                                                                                      <  2:8587228/151‑1 (MQ=255)

                                              aaGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAgg                                                                                                    <  2:3980186/151‑1 (MQ=255)

                                              aaGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAgg                                                                                                    >  2:8951197/1‑151 (MQ=255)

                                                  aGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCAt                                                                                                <  2:628102/151‑1 (MQ=255)

                                                   gCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATg                                                                                               <  2:4819887/151‑1 (MQ=255)

                                                       cttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCg                                                                                           >  1:1373786/1‑151 (MQ=255)

                                                        ttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGa                                                                                          <  1:10115804/151‑1 (MQ=255)

                                                        ttcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGa                                                                                          >  1:3610123/1‑151 (MQ=255)

                                                         tcATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAg                                                                                         <  1:7134998/151‑1 (MQ=255)

                                                          cATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGc                                                                                        <  1:4239063/151‑1 (MQ=255)

                                                            tGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGccc                                                                                      <  1:7557328/151‑1 (MQ=255)

                                                              cgcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTg                                                                                    <  1:9127817/151‑1 (MQ=255)

                                                               gcCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgc                                                                                   >  2:2546062/1‑151 (MQ=255)

                                                                ccccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgcg                                                                                  <  2:1805409/151‑1 (MQ=255)

                                                                 cccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGACTGCGCCGTTGGCGCGGTACACAGAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgcgc                                                                                 <  2:9562859/151‑1 (MQ=255)

                                                                 cccGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTgcgc                                                                                 >  2:9254733/1‑151 (MQ=255)

                                                                    gCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAg                                                                              >  1:9838725/1‑151 (MQ=255)

                                                                         tctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGcc                                                                         <  1:10008246/151‑1 (MQ=255)

                                                                          ctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCt                                                                        >  2:6524938/1‑151 (MQ=255)

                                                                          ctTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCt                                                                        >  1:8017249/1‑151 (MQ=255)

                                                                            tCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTgg                                                                      <  2:6637623/151‑1 (MQ=255)

                                                                                gcccagcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAAt                                                                  >  1:3299831/1‑151 (MQ=255)

                                                                                  ccagcGACCAGAGACTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCa                                                                <  2:714116/151‑1 (MQ=255)

                                                                                    agcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGc                                                              >  1:3468229/1‑151 (MQ=255)

                                                                                    agcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGc                                                              <  2:4239343/151‑1 (MQ=255)

                                                                                     gcGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCt                                                             <  1:7273659/151‑1 (MQ=255)

                                                                                      cGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTc                                                            <  1:2798369/151‑1 (MQ=255)

                                                                                      cGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTc                                                            <  1:6200075/151‑1 (MQ=255)

                                                                                        aCCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGTGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgg                                                          <  1:176024/151‑1 (MQ=255)

                                                                                        aCCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgg                                                          >  1:5265118/1‑151 (MQ=255)

                                                                                         ccAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACCCGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCggg                                                         <  2:4997537/151‑1 (MQ=255)

                                                                                         ccAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCggg                                                         <  2:803228/151‑1 (MQ=255)

                                                                                          cAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCgggg                                                        >  1:5646980/1‑151 (MQ=255)

                                                                                                cTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAAc                                                  <  2:6963522/151‑1 (MQ=255)

                                                                                                 tGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACg                                                 >  1:6748603/1‑151 (MQ=255)

                                                                                                 tGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACg                                                 >  2:2718673/1‑151 (MQ=255)

                                                                                                       aGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCa                                           >  2:4033218/1‑151 (MQ=255)

                                                                                                       aGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCa                                           >  1:1541606/1‑151 (MQ=255)

                                                                                                          cGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgcg                                        <  1:8969646/151‑1 (MQ=255)

                                                                                                           gATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgcgc                                       <  1:2030602/151‑1 (MQ=255)

                                                                                                           gATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgcgc                                       >  1:8476500/1‑151 (MQ=255)

                                                                                                           gATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAgcgc                                       >  2:10580379/1‑151 (MQ=255)

                                                                                                             tCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGa                                     >  1:9574968/1‑151 (MQ=255)

                                                                                                               gAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATg                                   >  1:7926137/1‑151 (MQ=255)

                                                                                                                 aTTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTa                                 >  2:3768592/1‑151 (MQ=255)

                                                                                                                    cGCCGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGcc                              >  2:10292837/1‑151 (MQ=255)

                                                                                                                      ccGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCCGCTCGGGGCCGCACGATACCAGCGCGATGTAGCCGt                            >  2:5395592/1‑151 (MQ=255)

                                                                                                                      ccGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGt                            <  1:2391154/151‑1 (MQ=255)

                                                                                                                       cGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTCGCCGTg                           >  2:6746479/1‑151 (MQ=255)

                                                                                                                       cGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTg                           >  1:4173739/1‑151 (MQ=255)

                                                                                                                       cGTTGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTg                           <  2:10780314/151‑1 (MQ=255)

                                                                                                                          tGGCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGaa                        <  1:10344419/151‑1 (MQ=255)

                                                                                                                           ggCGCGGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAAc                       <  1:1099072/151‑1 (MQ=255)

                                                                                                                             cgcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGAAGAACGATACCAGCGCGATGTAGCCGTGGAACGt                     >  1:3748867/1‑151 (MQ=255)

                                                                                                                              gcgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTg                    <  1:823251/151‑1 (MQ=255)

                                                                                                                               cgGTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGACCGTgg                   >  1:6086072/1‑151 (MQ=255)

                                                                                                                                ggTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCACTCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGc                  >  2:2477398/1‑151 (MQ=255)

                                                                                                                                ggTACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGc                  <  2:570907/151‑1 (MQ=255)

                                                                                                                                  tACACATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCa                <  1:3354090/151‑1 (MQ=255)

                                                                                                                                   acacATAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAg               >  2:766404/1‑151 (MQ=255)

                                                                                                                                     acaTAGAGCGCCGACAA‑GGCACAGAAGAACTCGAACACGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGa             <  1:6553220/151‑1 (MQ=255)

                                                                                                                                     acaTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGa             >  2:883432/1‑151 (MQ=255)

                                                                                                                                      caTAGTCCGCCGACAA‑CGCCCAGTACTACTCGAACCCGCACACGCCGGCCGCGTCCCCGATGGTCATGTTCGAGCCCTTCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCATCGCGATGTAGCCGTGGAACGTGGCCAGGAt            <  2:3189845/151‑1 (MQ=255)

                                                                                                                                      caTAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGAt            <  2:7850115/151‑1 (MQ=255)

                                                                                                                                        tAGAGCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGAGACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATca          <  1:777116/151‑1 (MQ=255)

                                                                                                                                           agCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATcagca       <  1:10581312/151‑1 (MQ=255)

                                                                                                                                           agCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATcagca       >  1:11193234/1‑151 (MQ=255)

                                                                                                                                           agCGCCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATcagca       <  2:11174893/151‑1 (MQ=255)

                                                                                                                                              gcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagga    >  1:3046597/1‑151 (MQ=255)

                                                                                                                                              gcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagga    >  1:10770771/1‑151 (MQ=255)

                                                                                                                                              gcCGACAA‑GGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCagcagga    >  1:4010656/1‑151 (MQ=255)

CGGGCGAGGCCGACAGCAGGCCGGAGTGCAGCGACAACGCCAGGGGAAGGAGCTTCTTCATGCGCCCCGCTCCTCTTCCAGCCCAGCGACCAGAGCCTGGGTCAGGCGATCGAATTCGCCGTTGGCGCGGTACACATAGAGCGCCGACAAGGGCACAGAAGAACACGAACAGGAACACGCCGGCCGCGACCCCGAAGGTCATGTTCGAGCCCTGCGCCAGACGCCTGGCAATCAGCTCGGGGCAGAACGATACCAGCGCGATGTAGCCGTGGAACGTGGCCAGGATCAGCAGGAGC  >  NC_008463/3197994‑3198289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`