BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,670,537	Δ1 bp	coding (537/1020 nt)	PA14_RS16675 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,670,534	0	T	.	99.7%	1819.8 / ‑3.3	371	coding (534/1020 nt)	PA14_RS16675	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (1/0); major base . (193/176); minor base C (1/0); total (195/176)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 383 total aligned reads displayed.

CCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTG‑G‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCC  >  NC_008463/3670386‑3670679

cccGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGttt                                                                                                                                                    <  1:7612022/151‑1 (MQ=255)

cccGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGttt                                                                                                                                                    >  2:5707898/1‑151 (MQ=255)

  cgcgACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGtttcc                                                                                                                                                  >  1:836257/1‑149 (MQ=255)

      aCGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑G‑a                                                                                                                                           >  2:2337253/1‑151 (MQ=255)

          gCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑G‑aagtc                                                                                                                                       >  2:178497/1‑147 (MQ=255)

           cTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑G‑aagtct                                                                                                                                      <  1:3489131/151‑6 (MQ=255)

            tCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑G‑ACGTCTg                                                                                                                                     >  2:7791452/1‑151 (MQ=255)

             cGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCGTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTgg                                                                                                                                    <  2:9510838/151‑1 (MQ=255)

               cccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTc                                                                                                                                  <  2:352155/151‑1 (MQ=255)

               cccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTc                                                                                                                                  <  1:3386378/151‑1 (MQ=255)

               cccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTc                                                                                                                                  <  2:560636/151‑1 (MQ=255)

                  gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCggg                                                                                                                               >  1:2223791/1‑151 (MQ=255)

                      gATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTg                                                                                                                           >  2:4776913/1‑151 (MQ=255)

                          tACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCgg                                                                                                                       >  2:3133046/1‑151 (MQ=255)

                           aCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGt                                                                                                                      <  1:1947294/151‑1 (MQ=255)

                           aCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGt                                                                                                                      <  1:3150245/151‑1 (MQ=255)

                             ggCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGc                                                                                                                    >  2:6312958/1‑151 (MQ=255)

                                ttCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGATGTGTCGACCAGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGCTCGGGGCTGCCGGTGctct                                                                                                                 >  2:10173053/1‑151 (MQ=255)

                                 tCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTa                                                                                                                <  1:3228164/151‑1 (MQ=255)

                                 tCCGCGTGTCGATCCTCTTCGCCCTCTCCCTGACCGTGCGCTGTTATCTGATCGGCGTCTCCGTCGGCGACCTGCTAGTCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTa                                                                                                                <  2:5222643/151‑1 (MQ=255)

                                  ccGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGCA‑A‑GTCTGGACGGGGCTGCCGGTGCTCTAc                                                                                                               >  2:8995532/1‑151 (MQ=255)

                                    gcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGGCCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACct                                                                                                             >  2:10880910/1‑151 (MQ=255)

                                      gtgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                           >  1:5784151/1‑151 (MQ=255)

                                       tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                          >  2:7026537/1‑151 (MQ=255)

                                       tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                          <  1:115436/151‑1 (MQ=255)

                                       tgtCGATCCTCTTCGCCCTGACCCTGACAGTGCTCAGTTCTCTGATCGGCGTCACCCACGGCGCCCTGCAATGCTACTACGGCGGCTGGGTCGACCAGCACGGCCAGCG‑ATACATG‑GAA‑GTCAGGTAGGGGCTGCCGGTGCAATACctgct                                                                                                          >  2:5061477/1‑151 (MQ=255)

                                         tCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGa                                                                                                        >  1:918642/1‑151 (MQ=255)

                                           gATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatc                                                                                                      >  2:3406000/1‑151 (MQ=255)

                                           gATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGC‑CTTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatc                                                                                                      >  2:7468116/1‑151 (MQ=255)

                                            aTCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatca                                                                                                     >  2:4557820/1‑151 (MQ=255)

                                            aTCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatca                                                                                                     <  2:689394/151‑1 (MQ=255)

                                             tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCTGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCGCGGCCCGCG‑TGTCCTG‑GTA‑GTCTGGTCGGGGCTGCCGGTGATCTACCTGCTGatcat                                                                                                    >  2:2727900/1‑151 (MQ=255)

                                               ctctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATcc                                                                                                  >  1:3743754/1‑151 (MQ=255)

                                                tctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCt                                                                                                 <  1:8852300/151‑1 (MQ=255)

                                                 ctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTg                                                                                                <  2:9272832/151‑1 (MQ=255)

                                                 ctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTg                                                                                                >  2:9715234/1‑151 (MQ=255)

                                                   tCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGc                                                                                              >  2:612485/1‑151 (MQ=255)

                                                     gCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCa                                                                                            >  1:1229332/1‑151 (MQ=255)

                                                      ccctgaccctgaCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                           <  2:10548342/151‑1 (MQ=255)

                                                      ccctgaccctgaCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                           <  2:8357853/151‑1 (MQ=255)

                                                       cctgaccctgacCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGc                                                                                          <  1:9440761/151‑1 (MQ=255)

                                                        ctgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCt                                                                                         <  2:8612518/151‑1 (MQ=255)

                                                         tgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                        <  1:6046851/151‑1 (MQ=255)

                                                         tgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                        >  1:11021959/1‑151 (MQ=255)

                                                            ccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGt                                                                                     >  1:4712252/1‑151 (MQ=255)

                                                            ccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCGGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑G‑ACGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGt                                                                                     >  1:10352363/1‑151 (MQ=255)

                                                              ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTcc                                                                                   >  2:4293788/1‑151 (MQ=255)

                                                              ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTcc                                                                                   <  1:2150997/151‑1 (MQ=255)

                                                                gaccGTGGTCAGGTCTCTGATCGGCGTCACCTTCGGCGCCCTGCACGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                 <  2:9894881/151‑1 (MQ=255)

                                                                gaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                 >  1:3297598/1‑151 (MQ=255)

                                                                  ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGcc                                                                               <  2:6455725/151‑1 (MQ=255)

                                                                  ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGcc                                                                               <  1:10859786/151‑1 (MQ=255)

                                                                   cGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCg                                                                              >  2:1981687/1‑151 (MQ=255)

                                                                      gCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAAc                                                                           >  2:5859523/1‑151 (MQ=255)

                                                                         cAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTc                                                                        >  2:9843633/1‑151 (MQ=255)

                                                                            ttCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtgg                                                                     <  1:8008108/151‑1 (MQ=255)

                                                                                ctGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgc                                                                 >  1:7557576/1‑151 (MQ=255)

                                                                                  gATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctg                                                               <  1:2431703/151‑1 (MQ=255)

                                                                                  gATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctg                                                               >  1:5867745/1‑151 (MQ=255)

                                                                                  gATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctg                                                               >  1:444373/1‑151 (MQ=255)

                                                                                   aTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgc                                                              >  2:2181965/1‑151 (MQ=255)

                                                                                    tCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgct                                                             <  1:1995571/151‑1 (MQ=255)

                                                                                         gTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGca                                                        >  1:4367180/1‑151 (MQ=255)

                                                                                            aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCGGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                     <  1:1409915/151‑1 (MQ=255)

                                                                                             ccGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatcat                                                    <  1:976609/151‑1 (MQ=255)

                                                                                              cGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtg                                                   >  1:5015777/1‑151 (MQ=255)

                                                                                               gTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgc                                                  <  1:3849800/151‑1 (MQ=255)

                                                                                                tCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgct                                                 <  1:1000418/151‑1 (MQ=255)

                                                                                                     gcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                            <  1:2710863/151‑1 (MQ=255)

                                                                                                      cccTGCACGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGtt                                           <  2:2289784/151‑1 (MQ=255)

                                                                                                      cccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGtt                                           <  1:7153094/151‑1 (MQ=255)

                                                                                                        cTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGtttt                                         >  1:9027319/1‑151 (MQ=255)

                                                                                                          gCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCAT‑CTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCt                                      >  2:2604313/1‑151 (MQ=255)

                                                                                                              ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGa                                   <  1:2996839/151‑1 (MQ=255)

                                                                                                                ctactaCGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAGGCTGCTGTTTTCCTGGATg                                 >  2:1598236/1‑151 (MQ=255)

                                                                                                                  actacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAg                               >  1:10608635/1‑151 (MQ=255)

                                                                                                                     acGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCt                            >  1:5892831/1‑151 (MQ=255)

                                                                                                                     acGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCt                            <  2:5413259/151‑1 (MQ=255)

                                                                                                                      cggcggCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtg                           >  1:7264300/1‑151 (MQ=255)

                                                                                                                       ggcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                          <  2:1111086/151‑1 (MQ=255)

                                                                                                                       ggcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                          >  1:5217452/1‑151 (MQ=255)

                                                                                                                         cggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtg                        >  1:8247195/1‑151 (MQ=255)

                                                                                                                          ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                       <  1:5094673/151‑1 (MQ=255)

                                                                                                                          ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                       >  1:5485708/1‑151 (MQ=255)

                                                                                                                           gcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGa                      <  1:8570449/151‑1 (MQ=255)

                                                                                                                            cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                     <  1:6279734/151‑1 (MQ=255)

                                                                                                                              gggTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGt                   >  1:8151931/1‑151 (MQ=255)

                                                                                                                               ggTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTg                  <  2:7306655/151‑1 (MQ=255)

                                                                                                                                gTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTgg                 <  1:9383541/151‑1 (MQ=255)

                                                                                                                                   gACCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc              >  1:460263/1‑151 (MQ=255)

                                                                                                                                    aCCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg             <  2:10480942/151‑1 (MQ=255)

                                                                                                                                    aCCTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg             <  1:10395422/151‑1 (MQ=255)

                                                                                                                                     ccTGCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgc            <  1:5369932/151‑1 (MQ=255)

                                                                                                                                        gCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc         >  2:7138914/1‑151 (MQ=255)

                                                                                                                                        gCTCGGCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc         >  2:1232754/1‑151 (MQ=255)

                                                                                                                                            ggCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt     >  1:10074180/1‑151 (MQ=255)

                                                                                                                                             gCCAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt    <  2:9585239/151‑1 (MQ=255)

                                                                                                                                              ccAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtc   <  1:6784851/151‑1 (MQ=255)

                                                                                                                                               cAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcc  >  2:3803618/1‑151 (MQ=255)

                                                                                                                                               cAGCG‑TTTCCTG‑GAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcc  >  1:1770128/1‑151 (MQ=255)

CCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTG‑G‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCC  >  NC_008463/3670386‑3670679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`