Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,514,060 (G)5→4 intergenic (+15/+136) PA14_RS25260 → / ← PA14_RS25265 tRNA‑Met/HigA family addiction module antitoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,514,0560G.100.0% 2053.0 / NA 414intergenic (+11/+140)PA14_RS25260/PA14_RS25265tRNA‑Met/HigA family addiction module antitoxin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (223/191);  total (223/191)

Only 100 of 447 total aligned reads displayed.

AGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAGGGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATCCG  >  NC_008463/5513906‑5514202
                                                                                                                                                      |                                                                                                                                                  
aGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAg                                                                                                                                                    <  2:4337254/151‑1 (MQ=255)
aGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAg                                                                                                                                                    <  2:4656473/151‑1 (MQ=255)
   aaaTTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAgggg                                                                                                                                                 <  2:5721201/151‑1 (MQ=255)
    aaTTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggt                                                                                                                                                >  2:1882189/1‑150 (MQ=255)
      gtATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggtta                                                                                                                                              <  1:5979606/150‑4 (MQ=255)
       tATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAggggttag                                                                                                                                             >  2:3021324/1‑147 (MQ=255)
        aTCGAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACGCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAgc                                                                                                                                           <  1:7271987/151‑1 (MQ=255)
          cTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAgcgc                                                                                                                                         <  2:3778235/151‑1 (MQ=255)
              gCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAAGGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGc                                                                                                                                     <  2:6303935/151‑1 (MQ=255)
                ccAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTa                                                                                                                                   <  2:8161882/151‑1 (MQ=255)
                ccAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTa                                                                                                                                   >  1:5424359/1‑151 (MQ=255)
                ccAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTa                                                                                                                                   <  1:10540782/151‑1 (MQ=255)
                     ggTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAAcccc                                                                                                                              >  2:1450758/1‑151 (MQ=255)
                     ggTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAAcccc                                                                                                                              <  1:11229760/151‑1 (MQ=255)
                      gTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCt                                                                                                                             <  1:5669793/151‑1 (MQ=255)
                        tGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCttt                                                                                                                           >  1:2384608/1‑151 (MQ=255)
                        tGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCttt                                                                                                                           >  2:1371748/1‑151 (MQ=255)
                         gACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCtttt                                                                                                                          <  1:5588601/151‑1 (MQ=255)
                          aCAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCttttt                                                                                                                         <  1:337940/151‑1 (MQ=255)
                          aCAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACAGAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCttttt                                                                                                                         <  1:66993/151‑1 (MQ=255)
                           cAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATATTGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttg                                                                                                                        <  2:1190991/151‑1 (MQ=255)
                            aGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGGTAACCCCTttttgt                                                                                                                       <  2:6408161/151‑1 (MQ=255)
                            aGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttgt                                                                                                                       >  2:5447778/1‑151 (MQ=255)
                               cccgcccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttgtttt                                                                                                                    >  1:2563726/1‑151 (MQ=255)
                               cccgcccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttgtttt                                                                                                                    >  1:3820695/1‑151 (MQ=255)
                               cccgcccgAGATACGGAAAATGCGCGCCACGCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCAGTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGGGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTttttgtttt                                                                                                                    <  2:9578255/151‑1 (MQ=255)
                                  gcccgAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGt                                                                                                                 <  2:11187062/151‑1 (MQ=255)
                                      gagaTACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGc                                                                                                             <  1:981508/151‑1 (MQ=255)
                                        gaTACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAg                                                                                                           <  1:1089704/151‑1 (MQ=255)
                                        gaTACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAg                                                                                                           <  2:7078335/151‑1 (MQ=255)
                                               aaaaTGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaa                                                                                                    >  1:271070/1‑151 (MQ=255)
                                                  aTGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaacc                                                                                                 <  1:9953017/151‑1 (MQ=255)
                                                  aTGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaacc                                                                                                 <  1:8852395/151‑1 (MQ=255)
                                                     cgcgcCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgac                                                                                              <  1:4228780/151‑1 (MQ=255)
                                                     cgcgcCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgac                                                                                              >  2:10355317/1‑151 (MQ=255)
                                                           ccTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactacaaa                                                                                        <  2:10946686/150‑1 (MQ=255)
                                                           aCTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactacaaa                                                                                        <  1:4678547/151‑1 (MQ=255)
                                                           aCTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactacaaa                                                                                        >  1:10738830/1‑151 (MQ=255)
                                                             tCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAgactacaaaccgactacaaacc                                                                                      <  1:4523110/151‑1 (MQ=255)
                                                              cGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGactacaaaccgactacaaaccg                                                                                     >  2:8778868/1‑151 (MQ=255)
                                                                gCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTc                                                                                   >  1:7715259/1‑151 (MQ=255)
                                                                 cTACATAGCTCAGTCGTTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCt                                                                                  <  2:8518517/151‑1 (MQ=255)
                                                                 cTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCCCCAGATTTGTCAA‑GGGGTTAGCGCAAGCTAACCACTTTTTGTTTTGGTCGGCAGACTACAGACCGACTCCAAACCGTCt                                                                                  >  2:6704126/1‑151 (MQ=255)
                                                                 cTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCt                                                                                  >  2:1644240/1‑151 (MQ=255)
                                                                   aCATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTAc                                                                                <  2:3344541/151‑1 (MQ=255)
                                                                    cATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCCGGTTCAAGTCCCGGTGTAGCCACCATAGTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACg                                                                               <  2:5942317/151‑1 (MQ=255)
                                                                    cATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACg                                                                               <  1:1017588/151‑1 (MQ=255)
                                                                    cATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACg                                                                               >  2:5266456/1‑151 (MQ=255)
                                                                       aGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGccc                                                                            <  1:7540092/151‑1 (MQ=255)
                                                                       aGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGccc                                                                            <  2:8394392/151‑1 (MQ=255)
                                                                       aGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGccc                                                                            <  2:3180930/151‑1 (MQ=255)
                                                                       aGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTGTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGccc                                                                            >  2:10808534/1‑151 (MQ=255)
                                                                           cAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCtt                                                                        >  1:6586627/1‑151 (MQ=255)
                                                                             gTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTa                                                                      <  1:7581073/151‑1 (MQ=255)
                                                                              tCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTAc                                                                     >  1:4931891/1‑151 (MQ=255)
                                                                              tCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTAc                                                                     <  1:9231608/151‑1 (MQ=255)
                                                                                  ttAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGAt                                                                 <  1:2846401/151‑1 (MQ=255)
                                                                                   tAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTATACCGAta                                                                >  2:10150773/1‑150 (MQ=255)
                                                                                    agagCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGAttt                                                               <  1:7395013/151‑1 (MQ=255)
                                                                                    agagCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGAttt                                                               <  1:3158752/151‑1 (MQ=255)
                                                                                      agCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATAATTTTCAA‑GGGGTTAGCGCAATCTATCCCCTTTTTGTTTTGTTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTAc                                                             >  2:6923528/1‑151 (MQ=255)
                                                                                       gcgcAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTCCCGATTTACg                                                            <  1:11087757/151‑1 (MQ=255)
                                                                                         gcagcaTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTGTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGt                                                          >  2:1794838/1‑151 (MQ=255)
                                                                                          cagcaTTCATAATGCTGATGTCCCAGGTGCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACGCCTTTTTGTTTTGGTCGGCAGACTACAATCCGACTACAAACCGTCTACGCCCCCTTTACCGAGATACGGtt                                                         >  2:10466656/1‑151 (MQ=255)
                                                                                           agcaTTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCAACATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTc                                                        <  2:6218106/151‑1 (MQ=255)
                                                                                                  aTAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaa                                                 >  2:9641505/1‑151 (MQ=255)
                                                                                                  aTAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaa                                                 >  2:1679165/1‑151 (MQ=255)
                                                                                                   tAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaaa                                                <  1:6319612/151‑1 (MQ=255)
                                                                                                     aTGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaaaaa                                              >  1:9468954/1‑151 (MQ=255)
                                                                                                     aTGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGaaaaa                                              <  2:4248215/151‑1 (MQ=255)
                                                                                                      tGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAg                                             <  2:2484695/151‑1 (MQ=255)
                                                                                                        cTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGcc                                           <  1:1037770/151‑1 (MQ=255)
                                                                                                        cTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGcc                                           <  1:7128324/151‑1 (MQ=255)
                                                                                                        cTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGcc                                           <  2:5152908/151‑1 (MQ=255)
                                                                                                         tGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGccc                                          >  1:8990839/1‑151 (MQ=255)
                                                                                                         tGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGccc                                          >  2:7825188/1‑151 (MQ=255)
                                                                                                          gATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGcccc                                         <  1:1543724/151‑1 (MQ=255)
                                                                                                           aTGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCg                                        <  2:922064/151‑1 (MQ=255)
                                                                                                            tGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGc                                       <  1:3651689/151‑1 (MQ=255)
                                                                                                             gTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCa                                      >  1:8398601/1‑151 (MQ=255)
                                                                                                              tCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCAt                                     <  1:2190316/151‑1 (MQ=255)
                                                                                                                  aGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGc                                 <  1:482222/151‑1 (MQ=255)
                                                                                                                    gTTCAAGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCgg                               >  1:4079132/1‑151 (MQ=255)
                                                                                                                        aaGTCCCGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGct                           <  1:5005155/151‑1 (MQ=255)
                                                                                                                           tCCCGGTGTAGCCACCATATTTTTCAA‑GGTGTTAGCGCAAGCTAACCCCATTTTGTTTTGGTCGGCAGACTACAAACCGACTACACACCGTCTACGCCCCCTTTAACGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTa                        <  2:5602369/151‑1 (MQ=255)
                                                                                                                             ccGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACg                      >  1:8098468/1‑151 (MQ=255)
                                                                                                                             ccGGTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACg                      >  1:1569588/1‑151 (MQ=255)
                                                                                                                               ggTGTAGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGAt                    >  1:6194824/1‑151 (MQ=255)
                                                                                                                                    aGCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACg               >  2:6144769/1‑151 (MQ=255)
                                                                                                                                     gCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGt              >  2:2271043/1‑151 (MQ=255)
                                                                                                                                     gCCACCATATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATGTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGt              >  2:7465573/1‑151 (MQ=255)
                                                                                                                                      ccaccaTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTc             <  1:5515309/151‑1 (MQ=255)
                                                                                                                                       ccccaTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCa            >  2:2712545/3‑151 (MQ=255)
                                                                                                                                       caccaTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCa            >  1:5143601/1‑151 (MQ=255)
                                                                                                                                       caccaTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTATACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCa            >  2:10596338/1‑151 (MQ=255)
                                                                                                                                          caTATTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCg         <  1:1947292/151‑1 (MQ=255)
                                                                                                                                           atatTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCATTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGc        <  1:6928648/151‑1 (MQ=255)
                                                                                                                                             atTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTa      >  2:7337000/1‑151 (MQ=255)
                                                                                                                                             atTTTTCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTa      >  1:3036598/1‑151 (MQ=255)
                                                                                                                                                tttCAA‑GGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATcc   <  2:4042559/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
AGAAAATTATCTAGGCCCAGGGGTTGACAGCCCCGCCCGAGATACGGAAAATGCGCGCCACTCGGCTACATAGCTCAGTCGGTTAGAGCGCAGCATTCATAATGCTGATGTCCCAGGTTCAAGTCCCGGTGTAGCCACCATATTTTTCAAGGGGGTTAGCGCAAGCTAACCCCTTTTTGTTTTGGTCGGCAGACTACAAACCGACTACAAACCGTCTACGCCCCCTTTACCGATTTACGGTTCGCATGAAAAAGCCCCGCATCTGCGGGGCTCTACGATCAACGTCACCGCTATCCG  >  NC_008463/5513906‑5514202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑