Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,441,861 (C)6→4 intergenic (+31/+11) mexK → / ← PA14_RS06750 multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,441,8570C.99.7% 1620.3 / ‑2.4 328intergenic (+27/+16)mexK/PA14_RS06750multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (162/165);  total (163/165)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0084631,441,8580C.99.7% 1620.3 / ‑2.3 328intergenic (+28/+15)mexK/PA14_RS06750multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (162/165);  minor base G (1/0);  total (163/165)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

CTTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGCCCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTCG  >  NC_008463/1441707‑1442005
                                                                                                                                                      ||                                                                                                                                                   
cttcGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGGTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTGCGTGGGAAGAAGGACGAGGCGCGGGCCTGTCGGGAGAAGGGGTCGGGGCGCTCgcgc                                                                                                                                                      >  2:8345077/1‑151 (MQ=255)
   cGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGcccc                                                                                                                                                   <  1:10261499/151‑1 (MQ=255)
   cGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGcccc                                                                                                                                                   >  1:4173078/1‑151 (MQ=255)
     ggCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgt                                                                                                                                                 >  1:10730312/1‑149 (MQ=255)
       ccGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtcc                                                                                                                                               <  2:4817939/151‑5 (MQ=255)
        cGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccg                                                                                                                                              <  1:8493855/151‑6 (MQ=255)
         gATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgt                                                                                                                                             >  2:4503921/1‑145 (MQ=255)
         gATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgt                                                                                                                                             >  1:5571378/1‑145 (MQ=255)
            ggCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGa                                                                                                                                        >  1:10082910/1‑151 (MQ=255)
             gCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGAc                                                                                                                                       >  1:9046710/1‑151 (MQ=255)
               ggTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTc                                                                                                                                     >  1:7173334/1‑151 (MQ=255)
               ggTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTc                                                                                                                                     >  1:3620525/1‑151 (MQ=255)
               ggTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTc                                                                                                                                     >  1:2275869/1‑151 (MQ=255)
               ggTGGCGATCAGGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTATATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGTGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTc                                                                                                                                     <  1:3065467/151‑1 (MQ=255)
               cgTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTc                                                                                                                                     >  2:5536036/2‑151 (MQ=255)
                  ggCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTTAAGAAGGACGAGGCGCGGGCCTGACGGGAGATGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCAcc                                                                                                                                  <  1:7488181/151‑1 (MQ=255)
                      aTCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGc                                                                                                                              <  2:10352206/151‑1 (MQ=255)
                      aTCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGc                                                                                                                              <  2:9654955/151‑1 (MQ=255)
                        cATGGGCGGGCAGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTCAGCCGCCTGGTGCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCcg                                                                                                                            <  1:2049173/151‑1 (MQ=255)
                         aTGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCcgc                                                                                                                           <  1:258441/151‑1 (MQ=255)
                          tGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCcgcg                                                                                                                          >  2:8615767/1‑151 (MQ=255)
                          tGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCcgcg                                                                                                                          >  1:6342507/1‑151 (MQ=255)
                            ggcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGATGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCTATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGcc                                                                                                                        <  1:5141555/151‑1 (MQ=255)
                            ggcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGcc                                                                                                                        >  2:7163378/1‑151 (MQ=255)
                            ggcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGcc                                                                                                                        <  1:5130222/151‑1 (MQ=255)
                            ggcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGcc                                                                                                                        <  1:1473810/151‑1 (MQ=255)
                               gggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGaa                                                                                                                     >  2:6647723/1‑151 (MQ=255)
                                  cTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACGCACCTGGCCGCGCCGAAGAc                                                                                                                  <  1:1098490/151‑1 (MQ=255)
                                   tGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGAcc                                                                                                                 >  1:9579399/1‑151 (MQ=255)
                                     aTCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACctt                                                                                                               >  1:3472630/1‑151 (MQ=255)
                                         tcgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTg                                                                                                           <  1:7458170/151‑1 (MQ=255)
                                          cgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTgg                                                                                                          >  2:2812053/1‑151 (MQ=255)
                                             cACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCa                                                                                                       >  2:5086869/1‑151 (MQ=255)
                                                 gTGCTCACCCTGCTGGTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGcc                                                                                                   <  1:1668519/151‑1 (MQ=255)
                                                  tGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCg                                                                                                  <  1:644654/151‑1 (MQ=255)
                                                    cTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGt                                                                                                <  2:9579328/151‑1 (MQ=255)
                                                     tCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTg                                                                                               >  1:6777975/1‑151 (MQ=255)
                                                        cccTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggcg                                                                                            <  2:1997522/151‑1 (MQ=255)
                                                           tgctgTTCCTGCCGGCGCTCTAGGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgcc                                                                                         <  1:3902768/151‑1 (MQ=255)
                                                            gctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCGTGGACAGGCCGGTGGCGgccg                                                                                        >  2:8977590/1‑151 (MQ=255)
                                                             ctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgc                                                                                       >  2:7303828/1‑151 (MQ=255)
                                                              tgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCCGGCCGGTGGCGgccgcc                                                                                      >  2:3992032/1‑151 (MQ=255)
                                                                ttCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgccgc                                                                                    >  1:1569044/1‑151 (MQ=255)
                                                                ttCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgccgc                                                                                    >  2:7016017/1‑151 (MQ=255)
                                                                 tCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgccgcc                                                                                   <  1:1275978/151‑1 (MQ=255)
                                                                  ccTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGccgccgccg                                                                                  <  2:4024081/151‑1 (MQ=255)
                                                                     gCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTGCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGt                                                                               >  2:3643813/1‑151 (MQ=255)
                                                                         gcgcTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGATGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTg                                                                           <  1:4665919/151‑1 (MQ=255)
                                                                           gcTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGACGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGc                                                                         >  2:4148488/1‑151 (MQ=255)
                                                                           gcTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGc                                                                         >  1:8900776/1‑151 (MQ=255)
                                                                           gcTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGc                                                                         >  2:10579902/1‑151 (MQ=255)
                                                                            ctctATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCCGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCg                                                                        >  1:1046210/1‑151 (MQ=255)
                                                                            ctctATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCg                                                                        >  1:1053770/1‑151 (MQ=255)
                                                                            ctctATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCg                                                                        <  1:4295870/151‑1 (MQ=255)
                                                                             tctATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCgg                                                                       >  1:896565/1‑151 (MQ=255)
                                                                               tATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGAt                                                                     >  2:6283616/1‑151 (MQ=255)
                                                                               tATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGAt                                                                     <  1:5330464/151‑1 (MQ=255)
                                                                                  gccgccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGCCGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGct                                                                  <  1:10612654/151‑1 (MQ=255)
                                                                                  gccgccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGct                                                                  <  2:5368528/151‑1 (MQ=255)
                                                                                    cgccTGTTTCCGTGTGAAGAAGTAGGTGGCGCGGGCCTGACGTGAGATGGGGACGGGGCTATCGGG‑‑CCCAGTCCGTCGACTCACCGGGCCGCGCCTAATACCTTCTTGGCCAGGCGGGTGGCTGCCGCCGCCGGGTTCTGGCGGATGcact                                                                <  1:5703078/151‑1 (MQ=255)
                                                                                    cgccTGGTTCGGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGGCCGTCGACTCACCTGGCCGCGCCGAAGAGCTGCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGctct                                                                >  2:7879467/1‑151 (MQ=255)
                                                                                    cgccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGctct                                                                <  2:993353/151‑1 (MQ=255)
                                                                                    cgccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGctct                                                                >  2:1071622/1‑151 (MQ=255)
                                                                                      ccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtcttc                                                              <  2:245893/151‑1 (MQ=255)
                                                                                       cTGGTTCCGTGTGAAGAAGGACGCGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtcttct                                                             <  1:9865482/151‑1 (MQ=255)
                                                                                           ttCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTg                                                         >  2:8510512/1‑151 (MQ=255)
                                                                                           ttCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTg                                                         >  1:6055934/1‑151 (MQ=255)
                                                                                            tCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGc                                                        >  1:4955238/1‑151 (MQ=255)
                                                                                             ccGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCt                                                       <  2:4281012/151‑1 (MQ=255)
                                                                                             ccGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCGCCTGCt                                                       <  2:8692257/151‑1 (MQ=255)
                                                                                               gtgtGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGGTCTGGCGGATGCTCTTCTCCTGCTTg                                                     >  2:7600843/1‑151 (MQ=255)
                                                                                                tgtgAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTgg                                                    <  2:140502/151‑1 (MQ=255)
                                                                                                  tgAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCg                                                  >  1:2455084/1‑151 (MQ=255)
                                                                                                         ggACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGatcatcg                                           >  1:7027580/1‑150 (MQ=255)
                                                                                                         ggACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCAtct                                           <  2:2932392/151‑1 (MQ=255)
                                                                                                          gACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCTGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCAtctc                                          <  1:8207049/151‑1 (MQ=255)
                                                                                                           aCGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCg                                         >  1:6310199/1‑151 (MQ=255)
                                                                                                            cGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGa                                        <  2:1236552/151‑1 (MQ=255)
                                                                                                             gAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCCGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGAGGCTCTTCTCCTGCGTGGCGAGCATCTCGat                                       >  2:1168166/1‑150 (MQ=255)
                                                                                                             gAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGaa                                       >  2:6795230/1‑151 (MQ=255)
                                                                                                                  gcgGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGcc                                  <  1:6996495/151‑1 (MQ=255)
                                                                                                                  gcgGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGcc                                  <  1:4631758/151‑1 (MQ=255)
                                                                                                                   cgGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGccc                                 >  2:5766888/1‑151 (MQ=255)
                                                                                                                          gACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAgg                          <  2:10897321/151‑1 (MQ=255)
                                                                                                                            cGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGc                        <  2:9290619/151‑1 (MQ=255)
                                                                                                                             gggAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGcc                       <  1:5052171/151‑1 (MQ=255)
                                                                                                                                agaAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGt                    >  1:1952393/1‑151 (MQ=255)
                                                                                                                                  acggggACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTc                  <  1:786532/149‑1 (MQ=255)
                                                                                                                                  aaGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTc                  <  2:8183168/151‑1 (MQ=255)
                                                                                                                                   aGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCg                 <  1:7673416/151‑1 (MQ=255)
                                                                                                                                    ggggACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCgg                <  1:1855421/151‑1 (MQ=255)
                                                                                                                                     gggACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGt               <  1:1189072/151‑1 (MQ=255)
                                                                                                                                     gggACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGt               >  1:9193982/1‑151 (MQ=255)
                                                                                                                                      ggACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTg              >  2:4390327/1‑151 (MQ=255)
                                                                                                                                          ggggCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACAt          >  2:3351100/1‑151 (MQ=255)
                                                                                                                                            ggCGATCGCG‑‑CCCCGTCCGACGACTCACCTGGCCGCGCCGAAGACCGTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAg        <  2:5973691/151‑1 (MQ=255)
                                                                                                                                               gcgatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGACGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGc       <  2:2117901/146‑1 (MQ=255)
                                                                                                                                                cgatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGcc      >  1:2772915/5‑151 (MQ=255)
                                                                                                                                                cgatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGcc      >  2:2324788/5‑151 (MQ=255)
                                                                                                                                                  atcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCt    >  1:339951/3‑151 (MQ=255)
                                                                                                                                                      ||                                                                                                                                                   
CTTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGCCCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTCG  >  NC_008463/1441707‑1442005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑