Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,670,543 +A coding (543/1020 nt) PA14_RS16675 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,670,5421.A99.0% 1590.7 / 5.3 385coding (542/1020 nt)PA14_RS16675ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base . (4/0);  new base A (195/185);  total (200/185)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 399 total aligned reads displayed.

GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTG‑G‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687
                                                                                                                                                         |                                                                                                                                                 
gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTg‑g                                                                                                                                                    >  1:8138936/1‑151 (MQ=255)
gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTg‑g                                                                                                                                                    >  1:8355272/1‑151 (MQ=255)
gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTg‑g                                                                                                                                                    >  2:7513412/1‑151 (MQ=255)
gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTg‑g                                                                                                                                                    <  1:9442092/151‑1 (MQ=255)
      cTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑G‑aagtct                                                                                                                                             >  1:7596467/1‑146 (MQ=255)
       tCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTg                                                                                                                                             <  2:6377308/151‑1 (MQ=255)
       tCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTg                                                                                                                                             <  1:5788116/151‑1 (MQ=255)
       tCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGAGTCACCGGCGGAGCCCAGATAGGCTACTACGGCGGCTGGGTCGACCTGGTCGGCCAGCG‑TTTCCTG‑GAAGTCTg                                                                                                                                             >  2:4658638/1‑151 (MQ=255)
        cGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCGTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTgg                                                                                                                                            <  2:9510838/151‑1 (MQ=255)
          cccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTc                                                                                                                                          <  2:560636/151‑1 (MQ=255)
            cGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCgg                                                                                                                                        >  2:9704251/1‑151 (MQ=255)
             gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCggg                                                                                                                                       >  1:2223791/1‑151 (MQ=255)
             gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCggg                                                                                                                                       <  1:4430757/151‑1 (MQ=255)
              ggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCgggg                                                                                                                                      <  2:10321676/151‑1 (MQ=255)
                  aTTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGc                                                                                                                                  <  2:921186/151‑1 (MQ=255)
                      aCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGt                                                                                                                              <  1:6178415/151‑1 (MQ=255)
                      aCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGt                                                                                                                              <  1:1947294/151‑1 (MQ=255)
                         gCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGCCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGct                                                                                                                           <  2:2614906/151‑1 (MQ=255)
                         gCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGct                                                                                                                           <  1:4464832/151‑1 (MQ=255)
                           ttCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGctct                                                                                                                         <  2:2023764/151‑1 (MQ=255)
                           ttCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGATGTGTCGACCAGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGCTCGGGGCTGCCGGTGctct                                                                                                                         >  2:10173053/1‑151 (MQ=255)
                            tCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTa                                                                                                                        <  1:3228247/151‑1 (MQ=255)
                             ccGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGCA‑AGTCTGGACGGGGCTGCCGGTGCTCTAc                                                                                                                       >  2:8995532/1‑151 (MQ=255)
                              cgcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTAcc                                                                                                                      <  2:3268701/151‑1 (MQ=255)
                                cgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACctg                                                                                                                    >  1:3810611/1‑151 (MQ=255)
                                cgTGTCGATCCTCTTCGCCCTGACCCTGACAGTGCTCAGTTCACTGAACGGAGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACctg                                                                                                                    >  2:1086226/1‑151 (MQ=255)
                                 gtgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                                   >  2:1907171/1‑151 (MQ=255)
                                 gtgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                                   >  2:5948865/1‑151 (MQ=255)
                                  tgtCGATCCTCTTCGCCCTGACCCTGACAGTGCTCAGTTCTCTGATCGGCGTCACCCACGGCGCCCTGCAATGCTACTACGGCGGCTGGGTCGACCAGCACGGCCAGCG‑ATACATG‑GAAGTCAGGTAGGGGCTGCCGGTGCAATACctgct                                                                                                                  >  2:5061477/1‑151 (MQ=255)
                                   gtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgctg                                                                                                                 >  1:56573/1‑151 (MQ=255)
                                    tCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGa                                                                                                                >  1:918642/1‑151 (MQ=255)
                                      gATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGCTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGGCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatc                                                                                                              >  2:4846475/1‑151 (MQ=255)
                                        tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcat                                                                                                            >  1:5008347/1‑151 (MQ=255)
                                               cGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGcc                                                                                                     >  2:246014/1‑151 (MQ=255)
                                                gCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCa                                                                                                    >  2:3713561/1‑151 (MQ=255)
                                                gCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCa                                                                                                    <  1:2313666/151‑1 (MQ=255)
                                                 ccctgaccctgaCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                                   <  2:6259420/151‑1 (MQ=255)
                                                  cctgaccctgacCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGc                                                                                                  <  1:5401676/151‑1 (MQ=255)
                                                   ctgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCt                                                                                                 <  2:8612518/151‑1 (MQ=255)
                                                   ctgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCt                                                                                                 <  1:1189949/151‑1 (MQ=255)
                                                    tgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                                <  2:2217651/151‑1 (MQ=255)
                                                    tgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                                <  2:6797190/151‑1 (MQ=255)
                                                        cctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTc                                                                                            >  1:7513549/1‑151 (MQ=255)
                                                        cctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTc                                                                                            >  1:4315567/1‑151 (MQ=255)
                                                        cctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTc                                                                                            >  1:1230791/1‑151 (MQ=255)
                                                        cctgaccGTGCTCAGTTCTCTGAGCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTc                                                                                            <  2:8406242/151‑1 (MQ=255)
                                                         ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTcc                                                                                           <  1:2150997/151‑1 (MQ=255)
                                                           gaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                         <  2:9894225/151‑1 (MQ=255)
                                                            accGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGc                                                                                        >  2:5723310/1‑151 (MQ=255)
                                                             ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGcc                                                                                       <  1:10859786/151‑1 (MQ=255)
                                                              cGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCg                                                                                      >  1:5986823/1‑151 (MQ=255)
                                                              cGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCg                                                                                      <  1:5892451/151‑1 (MQ=255)
                                                                  cTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACt                                                                                  >  2:4367426/1‑151 (MQ=255)
                                                                         ctctGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtggtg                                                                           >  2:2303924/1‑151 (MQ=255)
                                                                            tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCAGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgct                                                                        >  1:3511399/1‑151 (MQ=255)
                                                                             gATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTAGCTGCTGATCATCCTGGCCAGCTTCGTGCAGCCGAACTGCTGGGGgcgg                                                                       >  2:10527719/1‑149 (MQ=255)
                                                                               tCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgct                                                                     <  1:10559778/151‑1 (MQ=255)
                                                                                 ggCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTgg                                                                   >  1:10394150/1‑151 (MQ=255)
                                                                                 ggCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTgg                                                                   <  2:1604629/151‑1 (MQ=255)
                                                                                   cGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGc                                                                 <  1:2070649/151‑1 (MQ=255)
                                                                                        ccGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatcat                                                            <  1:976609/151‑1 (MQ=255)
                                                                                           tCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgct                                                         <  1:1000418/151‑1 (MQ=255)
                                                                                                gcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                                    <  1:2710863/151‑1 (MQ=255)
                                                                                                   cTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGtttt                                                 >  1:9031426/1‑151 (MQ=255)
                                                                                                    tGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TAACCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCAACCTGCTGATCATCCTGGCCAGATTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTc                                                >  2:3451754/1‑151 (MQ=255)
                                                                                                      cAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCt                                              <  1:8875238/151‑1 (MQ=255)
                                                                                                      cAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCt                                              >  2:9822674/1‑151 (MQ=255)
                                                                                                         ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGa                                           <  2:3026811/151‑1 (MQ=255)
                                                                                                         ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGa                                           <  1:2996839/151‑1 (MQ=255)
                                                                                                         ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TATCCTG‑GAAGTCTCGTCGGGGCAGCCGGAGCTCTACCTGCAGATCAACCTGGCCAGCTTCGTCCAGCCGAACTTCTGGAGCCTGCAGGGCATCATGCTGCAGTTTTACTGGa                                           >  1:3383027/1‑151 (MQ=255)
                                                                                                          gCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGAt                                          <  1:11038914/151‑1 (MQ=255)
                                                                                                            tactacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGATGTCCTGGATGa                                        >  1:8212822/1‑151 (MQ=255)
                                                                                                                acGGCGGCTGGGTCGACCAGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCt                                    >  2:738501/1‑151 (MQ=255)
                                                                                                                  ggcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                                  <  2:1111086/151‑1 (MQ=255)
                                                                                                                  ggcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                                  >  1:5217452/1‑151 (MQ=255)
                                                                                                                     ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                               <  1:5094673/151‑1 (MQ=255)
                                                                                                                     ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtgg                               >  2:7047242/1‑151 (MQ=255)
                                                                                                                      gcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGa                              <  1:8570467/151‑1 (MQ=255)
                                                                                                                        tGGGTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACg                            >  1:1416246/1‑151 (MQ=255)
                                                                                                                          ggTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTg                          <  2:4099798/151‑1 (MQ=255)
                                                                                                                          ggTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGAACATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTg                          >  1:6468637/1‑151 (MQ=255)
                                                                                                                           gTCGACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTgg                         <  1:9383541/151‑1 (MQ=255)
                                                                                                                              gACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc                      >  1:467282/1‑151 (MQ=255)
                                                                                                                              gACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc                      <  2:5254713/151‑1 (MQ=255)
                                                                                                                              gACCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc                      >  1:6875233/1‑151 (MQ=255)
                                                                                                                              gACCAGCTCGGCCTGCG‑GTTCCTG‑GAAGTCGGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGCACTTCTGGTGGCTGCGGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc                      <  2:8203090/151‑1 (MQ=255)
                                                                                                                               aCCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg                     >  2:6954273/1‑151 (MQ=255)
                                                                                                                               aCCTGCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg                     >  1:10300026/1‑151 (MQ=255)
                                                                                                                                   gCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc                 >  2:1232754/1‑151 (MQ=255)
                                                                                                                                   gCTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc                 >  2:7138917/1‑151 (MQ=255)
                                                                                                                                    cTCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCg                <  1:317930/151‑1 (MQ=255)
                                                                                                                                     tCGGCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGa               >  1:7184411/1‑151 (MQ=255)
                                                                                                                                       ggCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt             >  1:10074180/1‑151 (MQ=255)
                                                                                                                                        gCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt            <  2:9585239/151‑1 (MQ=255)
                                                                                                                                        gCCAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt            >  1:3422984/1‑151 (MQ=255)
                                                                                                                                          cAGCG‑TTTCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcc          >  1:1770128/1‑151 (MQ=255)
                                                                                                                                            agcgtttCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcct         <  1:7941759/147‑1 (MQ=255)
                                                                                                                                             gcgtttCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctc        >  2:5421789/4‑151 (MQ=255)
                                                                                                                                              cgtttCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctcc       <  2:1431006/149‑1 (MQ=255)
                                                                                                                                                  tCCTG‑GAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCgg   <  2:9951969/151‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                                 
GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTG‑G‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑