Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463114,8650TC26.9% 1965.4 / inf 771Y529H (TAC→CAC) vgrG1btype VI secretion system spike protein VgrG1b
Reads supporting (aligned to +/- strand):  ref base T (253/308);  new base C (88/118);  total (344/427)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 776 total aligned reads displayed.

GGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGGCAGCAACG  >  NC_008463/114715‑115015
                                                                                                                                                      |                                                                                                                                                      
  cggcACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTac                                                                                                                                                      >  1:7270887/1‑151 (MQ=255)
     cACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCacgac                                                                                                                                                   <  2:7775814/151‑1 (MQ=25)
     cACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCacgac                                                                                                                                                   <  1:7876336/151‑1 (MQ=25)
     cACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTacgac                                                                                                                                                   <  1:9838373/151‑1 (MQ=255)
       cGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTAcgacga                                                                                                                                                 >  1:3303479/1‑151 (MQ=255)
        gCCGGCCAACGTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACgag                                                                                                                                                <  2:3351825/151‑1 (MQ=255)
         ccGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACgaga                                                                                                                                               <  2:4836842/151‑1 (MQ=25)
         ccGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACgaga                                                                                                                                               >  2:8586023/1‑151 (MQ=25)
         ccGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACgaga                                                                                                                                               >  1:9941492/1‑151 (MQ=255)
         ccGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACgaga                                                                                                                                               <  1:8685802/151‑1 (MQ=255)
          cGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGAc                                                                                                                                              >  2:4389922/1‑151 (MQ=255)
           ggCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGTGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACg                                                                                                                                             <  2:2704781/151‑1 (MQ=255)
           ggCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACg                                                                                                                                             >  1:4609911/1‑151 (MQ=255)
            gCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACgg                                                                                                                                            >  2:1973332/1‑151 (MQ=255)
             ccAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGt                                                                                                                                           >  2:5545786/1‑151 (MQ=255)
                   tCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCAtgt                                                                                                                                     >  1:2660820/1‑151 (MQ=255)
                        gagaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGc                                                                                                                                >  1:5090969/1‑151 (MQ=25)
                        gagaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGc                                                                                                                                >  1:6879109/1‑151 (MQ=255)
                         agaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCa                                                                                                                               >  2:7536552/1‑151 (MQ=25)
                         agaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCa                                                                                                                               >  2:1764818/1‑151 (MQ=25)
                         agaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCa                                                                                                                               <  2:6601093/151‑1 (MQ=255)
                           aTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACg                                                                                                                             >  2:8268243/1‑151 (MQ=255)
                           aTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACg                                                                                                                             <  2:4795823/151‑1 (MQ=255)
                             ccGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGAt                                                                                                                           <  1:3090509/151‑1 (MQ=255)
                              cGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATc                                                                                                                          >  1:8128575/1‑151 (MQ=25)
                               gCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCg                                                                                                                         >  1:5697564/1‑151 (MQ=255)
                                  tGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCAc                                                                                                                      <  1:4458512/151‑1 (MQ=25)
                                  tGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCAc                                                                                                                      >  1:1788545/1‑151 (MQ=25)
                                      ggaCAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCgag                                                                                                                  <  1:9324663/151‑1 (MQ=255)
                                        aCAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCANGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGAc                                                                                                                <  1:5884202/151‑1 (MQ=255)
                                        aCAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGAc                                                                                                                >  2:10927949/1‑151 (MQ=255)
                                         cAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGAcc                                                                                                               <  1:10041711/151‑1 (MQ=255)
                                          aagaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCg                                                                                                              >  1:2009726/1‑151 (MQ=25)
                                          aagaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCg                                                                                                              >  2:1408272/1‑151 (MQ=25)
                                          aagaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCg                                                                                                              >  2:10481742/1‑151 (MQ=25)
                                           agaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGt                                                                                                             <  1:10516184/151‑1 (MQ=25)
                                           agaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGt                                                                                                             <  2:3652118/151‑1 (MQ=255)
                                            gaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTg                                                                                                            <  1:9068497/151‑1 (MQ=255)
                                             aagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTgg                                                                                                           <  1:9493411/151‑1 (MQ=25)
                                              agGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGa                                                                                                          <  2:5089055/151‑1 (MQ=25)
                                              agGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGa                                                                                                          >  2:9501624/1‑151 (MQ=25)
                                                ggCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGaca                                                                                                        <  1:6535913/151‑1 (MQ=255)
                                                  cgcCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGacaac                                                                                                      >  2:9229210/1‑151 (MQ=255)
                                                   gcCGAGCAGTTGTTCAGCCATGCCGAGACGAACCAGGACTGCGAGGTCGCGAACGCCGATAGCCACTGGGACGGCCACGACCGGACCAAGACCATCGACTACGACGAGAAGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGacaaca                                                                                                     <  2:11112490/151‑1 (MQ=14)
                                                    ccGAGCAGTTGTTCCTCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGAcaacaa                                                                                                    <  2:8011121/151‑1 (MQ=255)
                                                     cGAGCAGTTGGTCATCCATGCCGAGAGGCACCAGGCCATCGAGGTCGAGAACGCCGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGACGCACGATCGCACCGAGCCCGTGGACaacaac                                                                                                   <  2:6175430/151‑1 (MQ=11)
                                                      gAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACg                                                                                                  >  1:6750902/1‑151 (MQ=255)
                                                       aGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACga                                                                                                 >  1:11066298/1‑151 (MQ=25)
                                                        tgaGTTTTTGATCCATGCGGAGATGAACGAGTACTTCGAGGTCGAGAACGAGGATAGCCACTGGGTCGTCCTAGACCGGACAAAGACCATCGACTACGACGAGACGGTGCCTGTGAAGCACGATCTCAACGAGTCCGTGGACAACAACgag                                                                                                <  2:2107170/149‑1 (MQ=255)
                                                        gCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACgag                                                                                                <  1:7782986/151‑1 (MQ=25)
                                                        gCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACgag                                                                                                >  2:5257098/1‑151 (MQ=255)
                                                         cAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACgaga                                                                                               >  2:3900817/1‑151 (MQ=25)
                                                         cAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACgaga                                                                                               >  1:7511962/1‑151 (MQ=25)
                                                                tCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAc                                                                                        >  1:4157844/1‑151 (MQ=16)
                                                                 cATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAAcc                                                                                       <  1:362203/151‑1 (MQ=255)
                                                                  aTCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCa                                                                                      >  1:8507/1‑151 (MQ=255)
                                                                  aTCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCa                                                                                      <  1:3827560/151‑1 (MQ=255)
                                                                     cATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtgc                                                                                   <  2:6048057/151‑5 (MQ=9)
                                                                     cATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCg                                                                                   <  2:1278481/151‑1 (MQ=255)
                                                                     cATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCg                                                                                   >  1:2541219/1‑151 (MQ=255)
                                                                      aTGCCGAGAAGTAACAGGACATCGCGGTCGAGAACTACGAGAGACACTGTGTCGGCCACGTACGGACAAAGACAATCGACTAAGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACATCGAGACCATTTCCTTCgg                                                                                  <  2:9032735/151‑1 (MQ=14)
                                                                      aTGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCgg                                                                                  >  1:1486950/1‑151 (MQ=255)
                                                                       tGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtgcat                                                                                 >  1:10883916/1‑145 (MQ=0)
                                                                        gCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCg                                                                                <  2:8138755/151‑1 (MQ=255)
                                                                          cGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTc                                                                              >  1:5529091/1‑151 (MQ=255)
                                                                          cGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTc                                                                              >  1:4389385/1‑151 (MQ=255)
                                                                                gaaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGc                                                                        <  2:6258746/151‑1 (MQ=255)
                                                                                   ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCAcc                                                                     <  2:9838260/151‑1 (MQ=255)
                                                                                    cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCg                                                                    >  1:5427814/1‑151 (MQ=255)
                                                                                             gAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCg                                                           <  1:2881392/151‑1 (MQ=255)
                                                                                                   gagaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTaaca                                                     >  2:2384880/1‑151 (MQ=255)
                                                                                                          acgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGagaa                                              >  1:9671130/1‑151 (MQ=255)
                                                                                                          acgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGagaa                                              >  1:3493034/1‑151 (MQ=255)
                                                                                                           cgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGagaag                                             <  2:3058563/151‑1 (MQ=255)
                                                                                                              gagCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGGCGACCGCACCGAGAAGGTCGGAAACAACGAGACGATc                                          >  1:8956871/1‑151 (MQ=255)
                                                                                                               agCCACAGGGTCGGCCACGACCGGACCAAGACCCTCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCa                                         <  2:10265744/151‑1 (MQ=255)
                                                                                                                   aCTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTAt                                     <  2:11209612/151‑1 (MQ=255)
                                                                                                                    cTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATc                                    <  2:93522/151‑1 (MQ=255)
                                                                                                                    cTGGGTCGGCCACGACCGGACCAAGACCAACGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATc                                    <  2:5283656/151‑1 (MQ=255)
                                                                                                                      gggTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGAAACACCGAGAAGATCAGTATCgg                                  >  2:8436912/1‑151 (MQ=255)
                                                                                                                       ggTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGgc                                 <  2:579672/151‑1 (MQ=255)
                                                                                                                        gTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGgcg                                <  1:2302211/151‑1 (MQ=255)
                                                                                                                        gTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGAAACAACGAGAAGATCAGTATCGgcg                                >  2:687043/1‑151 (MQ=255)
                                                                                                                             ccACGACCGGACCAAGACCATCGACTCCGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAAc                           <  2:3507971/151‑1 (MQ=255)
                                                                                                                             ccACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAAc                           >  1:3383853/1‑151 (MQ=255)
                                                                                                                             ccACGACCGGACCAAGACCATCGACTACGACGAGACGGTGAATGAGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAAc                           <  2:2561011/151‑1 (MQ=255)
                                                                                                                                 gACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCa                       <  2:4513017/151‑1 (MQ=255)
                                                                                                                                  aCCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCAc                      <  1:10332555/151‑1 (MQ=255)
                                                                                                                                   ccGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCAcc                     >  1:8396788/1‑151 (MQ=255)
                                                                                                                                     ggACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGa                   <  1:7083272/151‑1 (MQ=255)
                                                                                                                                       aCCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAgg                 <  1:1197090/151‑1 (MQ=255)
                                                                                                                                         cAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGAc               <  2:4061140/151‑1 (MQ=255)
                                                                                                                                         cAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGAc               >  1:7644647/1‑151 (MQ=255)
                                                                                                                                         cAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGAc               >  1:6235045/1‑151 (MQ=255)
                                                                                                                                            gACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACACCGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTc            <  2:1071567/151‑1 (MQ=255)
                                                                                                                                            gACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTc            <  2:720010/151‑1 (MQ=255)
                                                                                                                                             aCCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCg           <  2:7345421/151‑1 (MQ=255)
                                                                                                                                             aCCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCg           <  1:4827799/151‑1 (MQ=255)
                                                                                                                                                   gACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGgcagca     <  2:8929170/151‑1 (MQ=255)
                                                                                                                                                      tACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGGCACCAACg  >  2:10049116/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGGCAGCAACG  >  NC_008463/114715‑115015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑