Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463867,3780AC22.8% 880.8 / 19.7 404L390R (CTG→CGG) PA14_RS04080MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (234/51);  new base C (19/65);  total (253/151)
Fisher's exact test for biased strand distribution p-value = 1.17e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 413 total aligned reads displayed.

GCTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGCTGG  >  NC_008463/867228‑867528
                                                                                                                                                      |                                                                                                                                                      
gCTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCa                                                                                                                                                        >  1:10581983/1‑151 (MQ=255)
gCTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCa                                                                                                                                                        >  2:1151276/1‑151 (MQ=255)
gCTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCGGGCGTCAGCGTCGTCGCGCCa                                                                                                                                                        <  1:1950884/151‑1 (MQ=255)
gCTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCGGGATTTCGGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCa                                                                                                                                                        <  2:9628262/151‑1 (MQ=255)
 ctctATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAg                                                                                                                                                       <  2:8618178/151‑1 (MQ=255)
 ctctATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAg                                                                                                                                                       <  2:6605472/151‑1 (MQ=255)
   ctATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGgc                                                                                                                                                     <  1:6225165/151‑1 (MQ=255)
     aTCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGgcgc                                                                                                                                                   >  2:11113540/1‑151 (MQ=255)
        cGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGcgcgcg                                                                                                                                                >  1:9698035/1‑151 (MQ=255)
        cGTCGCACCGGCATCCGTCGACGAACGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGcgcgcg                                                                                                                                                <  1:2676690/151‑1 (MQ=255)
         gTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGcga                                                                                                                                               >  1:28054/1‑151 (MQ=255)
             cACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCgacgac                                                                                                                                           >  1:6519722/1‑151 (MQ=255)
              aCCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGacgacg                                                                                                                                          >  2:2370684/1‑151 (MQ=255)
                   ctcccGTCGACGCTCGTCCGGTGCTACCTGGTTTTCTGCCAGCAGCACCTGGACCTGCTGCCCGAGGCCTGTGGGCTGGCCGGCCCTGCGCCTGGCGCGGGGTTGGACGCGGGGGTCTGCGACGCCGCGCCAGGCCCGCGACGACGGGGag                                                                                                                                     <  2:8249125/148‑1 (MQ=255)
                    aTCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGGCGGGgcgc                                                                                                                                    >  1:8456015/1‑151 (MQ=255)
                    aTCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGagc                                                                                                                                    >  2:5093582/1‑151 (MQ=255)
                    aTCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGagc                                                                                                                                    >  1:11201282/1‑151 (MQ=255)
                      ccGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGagcag                                                                                                                                  >  2:1760631/1‑151 (MQ=255)
                       cGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCAGGCGTCAGCGTCGTCGCCCCCAGCGCGCGACGACGGGGatcagc                                                                                                                                 >  1:506616/1‑151 (MQ=255)
                        gTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTAGCGCCAGGCGCGCGACGACGGGGAGCAgcg                                                                                                                                >  1:2406895/1‑151 (MQ=255)
                          cgacgaTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGcc                                                                                                                              >  2:10782533/1‑151 (MQ=255)
                            acgaTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAg                                                                                                                            >  2:3719332/1‑151 (MQ=255)
                            acgaTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAg                                                                                                                            >  1:461725/1‑151 (MQ=255)
                               aTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACCACGGGGAGCAGCGCCAGAcc                                                                                                                         >  1:890953/1‑151 (MQ=255)
                                     tggTGCTCCGTGTTTTTCTGCCCGCAGCACCTGCCCCTGCTGCAGGTTGCGGGTCGGCTGGCCGGCCCTGCGCGTGGGGGGGGGTCGGCGGCTGGCGTCCGCGGGGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTAc                                                                                                                   <  2:1317402/150‑1 (MQ=255)
                                     aGGTGCTACCTGGATTTCTGCCAGCAGCGCCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGTCGCTGGCGGCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAggtc                                                                                                                   >  2:6546816/1‑148 (MQ=255)
                                     aGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTAc                                                                                                                   >  1:8350018/1‑151 (MQ=255)
                                      ggTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACg                                                                                                                  >  1:8123966/1‑151 (MQ=255)
                                       gTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGTGCAGCGCCAGACCGAGGACgg                                                                                                                 >  1:1014752/1‑151 (MQ=255)
                                           tACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAg                                                                                                             >  1:2651774/1‑151 (MQ=255)
                                            aCCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAgg                                                                                                            >  2:1638799/1‑151 (MQ=255)
                                            aCCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCNGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAgg                                                                                                            >  1:5879223/1‑151 (MQ=255)
                                               tGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGGGGGcc                                                                                                         >  1:4312998/1‑151 (MQ=255)
                                               tGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGcc                                                                                                         >  2:4996071/1‑151 (MQ=255)
                                                  aTTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGTGCAGCGCCAGACCGAGTACGGCGAGGGCCGCt                                                                                                      >  1:2315796/1‑151 (MQ=255)
                                                   tttCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGGGCAGCGCCAGACCCAGTACGGCGAGGGCCGCTg                                                                                                     >  1:306245/1‑151 (MQ=255)
                                                      cTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGTCCGAGTACGGCGAGGGCCGCTGcga                                                                                                  >  2:474450/1‑151 (MQ=255)
                                                       tGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCGGcgac                                                                                                 >  2:4879104/1‑151 (MQ=255)
                                                       tGCCAGCAGCACCCGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGGCGGGGCGCAGCGCCAGACCGAGTACGGCGTGGGGCGCTGcgac                                                                                                 >  1:4711185/1‑151 (MQ=255)
                                                         ccAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGGCAGGCGCGCGTCCACGGGGAGCAGCGCCAGACCCAGTACGGCGAGGGCCGCAGcgtcca                                                                                               >  1:177394/1‑147 (MQ=255)
                                                          cagcagCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGTCGaa                                                                                              >  1:9920583/1‑151 (MQ=255)
                                                              agcaCCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCg                                                                                          >  2:8362558/1‑151 (MQ=255)
                                                               gcaCCGGGACCGGCTGCACGTGGCCTGGCGGCTGGACGGCCCCGCGCCTGGCGCGGGGTTGGACGCGGGCGTCTGGTTCGTCGCGCCCGCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGa                                                                                         <  2:1408902/151‑1 (MQ=255)
                                                                cccgtgcaCCTGCTCCACGATGCCTGTGGGGGGGCCGGCCCTGGGCGTGGCGGGGGTTCGGCCGCGGGCGACCGGTTCGGCGCGCCAGCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGAt                                                                                        <  1:10876356/144‑1 (MQ=255)
                                                                caCCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGAt                                                                                        >  2:6945608/1‑151 (MQ=255)
                                                                 aCCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGGCGCGCCAGGCGCGCGACGCCGGGGCGCAGCGCCAGACCGAGGACGGCGAGGGCCGCTGCGACGAAGCCGagt                                                                                       >  1:6726066/1‑149 (MQ=255)
                                                                  ccTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGACGCCGATTc                                                                                      >  2:745606/1‑151 (MQ=255)
                                                                     ggcccTGCTGCACGATGCGTGTGGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGACTGCGACGCCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGtt                                                                                   <  2:11005748/148‑1 (MQ=255)
                                                                       aCCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCGGCGACGTAGCCGATTCGGTcc                                                                                 >  1:7044268/1‑151 (MQ=255)
                                                                          tggtgcCCGTTGCCGGTCGGCTGGACGCCCCGGCGCCTGGCGGGGGTTTGGACGGTGGCTTCTGGGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAgg                                                                              <  2:1052428/148‑1 (MQ=255)
                                                                          tgctgcACTATGCCTGGGGGGGGGACGGCCCTGCGCCTGGCGCGGGGTTGTACGCGGGCGTCTGCGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAgg                                                                              <  2:9094734/151‑1 (MQ=255)
                                                                                aCGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGCGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCgaga                                                                        >  1:10621105/1‑151 (MQ=255)
                                                                                  ttggcgtgtgGGCTGGCGGGCCGTGCGCGGGGGGGGGGGTTGGCGGGGGGCGTCTGCGGCGCCGCCCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGt                                                                      <  1:5297414/145‑1 (MQ=255)
                                                                                  gATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGt                                                                      >  2:387192/1‑151 (MQ=255)
                                                                                   aTGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGCGGGCCGCTGCGACGAAGCCGCTTCGTGCCAGGCCGAGAGtg                                                                     >  2:27700/1‑150 (MQ=255)
                                                                                   aTGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTc                                                                     >  2:2060475/1‑151 (MQ=255)
                                                                                    ggcgggTCGGCTGGACGGCCCTGCGCCGGGGGCGGGGTAGGCCGGGGGCGTCAGCGTCGCCGCCCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTcg                                                                    <  2:9153778/146‑1 (MQ=255)
                                                                                      ccTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGGGCAGCGCCAGACCGAGTACGGCGAGGGCCGCGGCGACGAAGCCGATTCGTTACAGGCCGAGAGTcgcg                                                                  >  1:480015/1‑151 (MQ=255)
                                                                                         gTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGGGCGCGACGACGGGGCGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATg                                                               >  2:9248325/1‑151 (MQ=255)
                                                                                         gTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGCCGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCGGCGACGAAGCCGATTCGTTCCAGGGCGTGAGGAGCGATg                                                               >  1:2268343/1‑151 (MQ=255)
                                                                                           cGGCTGTCCGCCCCTGCGCCGGGGGGGTGGTAGGAGGCTGGCGACTCCGTCTGCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGt                                                             <  1:3639496/151‑1 (MQ=255)
                                                                                           cGGCTGGCGGGCCCTGCGCGGGGGGGGGGGTTGGCCGGGGGCGCCTGGGTCGGCGCGCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGt                                                             <  1:3246914/151‑1 (MQ=255)
                                                                                             gCTGGACGGCCCTGCGCCTGGCGGGGGGGAGGACGCTGGCGTCTGCGTCGGCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtct                                                           <  2:1529334/151‑1 (MQ=255)
                                                                                              cTGGAGGGCCCGGCGCCGGGCGCGGGTTAGGACGCTGGCGTCCGGGGCGTCGCGCCAGGGGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGACGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctc                                                          <  2:2472767/151‑1 (MQ=255)
                                                                                                ggAGGGCCGTGCGCGGGGCGGGGGGTCGGACGCTGGCGTCCGCGTGGGCGCGCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctctc                                                        <  1:6309505/151‑1 (MQ=255)
                                                                                                ggACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctctc                                                        >  2:5461408/1‑151 (MQ=255)
                                                                                                ggACGGCCCTGCGCCGGGGGGGGGGTTGGACGGGGGCGTCCGCGTCGGCGCGCCAGCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctctc                                                        <  2:3290612/151‑1 (MQ=255)
                                                                                                  ccGGCCCTGCGCCTGGCGGGGGTTTGGACGCGGGCGTCAGCGGCGCCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCg                                                      <  2:8857523/150‑1 (MQ=255)
                                                                                                   cGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCGGCGACGAAGCCGATTCGTTCCCGGCCGAGAGTCGCGATGGTCTCTCCGc                                                     >  2:8103138/1‑151 (MQ=255)
                                                                                                    ggCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCTGCGTCGTCGCGCCAGGCGCGCGACGTCGGGGAGCCGCGCCAGACCGCGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGcc                                                    >  2:2565162/1‑151 (MQ=255)
                                                                                                    ggCCCTGCGCCGGGCGCGGGGTCGGACGCGGGCGCCCGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGcc                                                    <  2:5787537/151‑1 (MQ=255)
                                                                                                            gcCTGGCGGGGGGTTGGCCGGGGGCGTCAGCGTCGGCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgcg                                            <  2:10799153/151‑1 (MQ=255)
                                                                                                             cggggCGCGGGGTCGGCGGGTGGGGTCCGCGTCGGCGCGCTAGGGGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGTGGGCCGCGGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgcgc                                           <  2:6975223/148‑1 (MQ=255)
                                                                                                               tGGCGGGGGGTAGGCCGCTGGCGTCAGCGTCTACGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgcgcgc                                         <  2:4596251/151‑1 (MQ=255)
                                                                                                               tGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgagcgc                                         >  2:7088130/1‑151 (MQ=255)
                                                                                                               gggCGCGGGGTTGGCCGGTGGCTTCAGGTTCTTCGCGCCCGCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgcgcgc                                         <  1:9118640/150‑1 (MQ=255)
                                                                                                                ggCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGcc                                        >  2:79187/1‑151 (MQ=255)
                                                                                                                 gcggGGGGTAGGCGGGTGGCGTCCGCGCCTCCCCCCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCg                                       <  1:7825717/151‑1 (MQ=255)
                                                                                                                 gcgcGGGGTTGGACGCTGGCGTCTGCGTCGGCGCGCACGCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCg                                       <  2:4173386/151‑1 (MQ=255)
                                                                                                                  cgcgGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGa                                      >  2:6151986/1‑151 (MQ=255)
                                                                                                                     ggggTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGTAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGc                                   >  2:665960/1‑151 (MQ=255)
                                                                                                                     ggggTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGc                                   >  2:928304/1‑151 (MQ=255)
                                                                                                                      gggTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCACCGCCAAGTAGCGCGCCGAGGcc                                  >  2:5551611/1‑151 (MQ=255)
                                                                                                                           ggACGCGGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGGTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATcc                             <  2:290665/151‑1 (MQ=255)
                                                                                                                            gACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATccc                            >  2:8756670/1‑151 (MQ=255)
                                                                                                                             aCGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCCGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCg                           >  2:4059253/1‑151 (MQ=255)
                                                                                                                               ggtGGCGTCCGCGTGGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGc                         <  1:10008962/149‑1 (MQ=255)
                                                                                                                                cgggCGTCTGGGTCGCCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCa                        <  2:5643532/149‑1 (MQ=255)
                                                                                                                                 tGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGAACGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCAGCCAAGTAGCGCGCCGAGGCCGATCCCGGCAt                       >  2:10204684/1‑151 (MQ=255)
                                                                                                                                  ggCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCAtt                      <  2:7629102/151‑1 (MQ=255)
                                                                                                                                     gtctgggtcgtcGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggc                   <  2:7991123/145‑1 (MQ=255)
                                                                                                                                     gTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCCTTggc                   >  2:6225414/1‑151 (MQ=255)
                                                                                                                                         gCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcg               <  2:4389425/151‑1 (MQ=255)
                                                                                                                                         gCGTCGCCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcg               <  2:3077012/151‑1 (MQ=255)
                                                                                                                                           gtcgtcGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcggc             >  1:8647936/1‑151 (MQ=255)
                                                                                                                                            ccgtcgCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGgcggcggcg            <  2:1004941/150‑1 (MQ=255)
                                                                                                                                              gtcgCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGAc          >  1:9325207/1‑151 (MQ=255)
                                                                                                                                              gccgcgCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGAc          <  1:5447233/149‑1 (MQ=255)
                                                                                                                                                 gcgcCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGtt       >  2:5268759/1‑151 (MQ=255)
                                                                                                                                                    ccAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGCt    >  1:4405088/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GCTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGCTGG  >  NC_008463/867228‑867528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑