Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,829,3170CG22.8% 1074.2 / 64.8 418L7L (CTG→CTC) PA14_RS22165succinate dehydrogenase assembly factor 2
Reads supporting (aligned to +/- strand):  ref base C (63/245);  new base G (91/0);  total (173/245)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 424 total aligned reads displayed.

ACCGAACATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTATCCGCGC  >  NC_008463/4829167‑4829467
                                                                                                                                                      |                                                                                                                                                      
 ccGAACCTGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCa                                                                                                                                                       <  1:3026622/151‑1 (MQ=255)
   gcacctGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGCAGCGCGCCTGGTCCTCGTCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGt                                                                                                                                                     <  2:6383352/146‑1 (MQ=255)
   gAACATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGt                                                                                                                                                     <  1:3782172/151‑1 (MQ=255)
    aaCATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGtt                                                                                                                                                    <  1:4380438/151‑1 (MQ=255)
     acctGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTc                                                                                                                                                   <  2:3436214/148‑1 (MQ=255)
     aCATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTc                                                                                                                                                   <  2:5116038/151‑1 (MQ=255)
     aCATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTc                                                                                                                                                   <  2:6535140/151‑1 (MQ=255)
       aTGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTggg                                                                                                                                                 >  1:4119189/1‑151 (MQ=255)
               gTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTAGGGTTGAtat                                                                                                                                         >  1:6739061/1‑149 (MQ=255)
                tCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTGGGTTTGATATg                                                                                                                                        >  1:1817894/1‑151 (MQ=255)
                   tCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCa                                                                                                                                     <  1:8133375/151‑1 (MQ=255)
                    cGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCAt                                                                                                                                    <  2:7062398/151‑1 (MQ=255)
                     gCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATg                                                                                                                                   <  1:11144265/151‑1 (MQ=255)
                      cACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGCGTTCGGGTTAATATGCCCTGt                                                                                                                                  >  1:8747687/1‑151 (MQ=255)
                       aCTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGtt                                                                                                                                 <  1:6514474/151‑1 (MQ=255)
                        cTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTCGGGTTCATATTCCAGGttg                                                                                                                                >  1:7581633/1‑150 (MQ=255)
                          ccAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCgg                                                                                                                              <  1:460117/151‑1 (MQ=255)
                          ccAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCgg                                                                                                                              <  1:10391243/151‑1 (MQ=255)
                           cagcagCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTAGGGTTGATATGACATggtatgg                                                                                                                             >  1:2879209/1‑145 (MQ=255)
                               agcTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCATTTAGGTTGAATATGCCATGTTCGGGctag                                                                                                                         >  2:5936580/1‑149 (MQ=255)
                                gcTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGctcct                                                                                                                        <  2:129239/151‑1 (MQ=255)
                                    gCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGaa                                                                                                                    <  2:2730929/151‑1 (MQ=255)
                                    gCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGaa                                                                                                                    <  2:8537166/151‑1 (MQ=255)
                                    gCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGaa                                                                                                                    <  1:10659059/151‑1 (MQ=255)
                                        aaGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCTCATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGGTGGGTTTAATCTGCCCTGTTGGGGCTCCCGGGAAACg                                                                                                                >  1:8373826/1‑151 (MQ=255)
                                        aaGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACg                                                                                                                <  2:5270472/151‑1 (MQ=255)
                                            gcgcCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCg                                                                                                            <  1:3888402/151‑1 (MQ=255)
                                             cgcCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTAGGTTTAATCTGCCAAGTTCGGGCTCCACGAAAACGccccg                                                                                                           >  2:9315031/1‑149 (MQ=255)
                                              gcCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCCCCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCCGTAGAGTCGTGTCAGGTCGGGTTAAACTGGCAGGTTCGGGCGCCGCGGGAACGGCCggg                                                                                                          >  2:866649/1‑150 (MQ=255)
                                              gcCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGCGGTCGGTTTGATATTCCCAGTTAGGGCGCCTCGGAAACGCCCGGc                                                                                                          >  1:3986739/1‑151 (MQ=255)
                                              gcCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGATTTGGGTTTAATATGCCCAGTTAGGGCTCCGCGAAAAAGCCCGGc                                                                                                          >  1:9224741/1‑151 (MQ=255)
                                              gcCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTCGGGTTCATATGCCCAGTTCGGGCTCCTCGAAAACCCCCGGc                                                                                                          >  1:6393683/1‑151 (MQ=255)
                                                cTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCAt                                                                                                        <  1:4236442/151‑1 (MQ=255)
                                                  ggTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATta                                                                                                      <  2:4323140/151‑1 (MQ=255)
                                                  ggTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATta                                                                                                      <  1:2520664/151‑1 (MQ=255)
                                                  ggTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATta                                                                                                      <  1:150722/151‑1 (MQ=255)
                                                     ccTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAg                                                                                                   <  2:3374811/151‑1 (MQ=255)
                                                        cGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGc                                                                                                <  1:7102018/151‑1 (MQ=255)
                                                        cGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGGTCGGGTTGATCTGCCAGGTTCGGGCTCCTCGCAAACCCCCGGCCTTATAGGGc                                                                                                >  2:10811396/1‑151 (MQ=255)
                                                         ggCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCg                                                                                               <  1:3339525/151‑1 (MQ=255)
                                                           cGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGt                                                                                             <  1:5358257/151‑1 (MQ=255)
                                                              ccAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTCGGTTTCATCTGCCCTGTTCGGGCTCCTCGAAAACGCCCCGCCTTATAGGGCGGTTgc                                                                                          >  2:9227071/1‑151 (MQ=255)
                                                               cAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCTGTAGAGTCGTGTGCGGTAGGGTTGTTCTTCCATGGTCGGGGGCCGCCGAAACGCCCGGCAGTTTAGGGGGGGTgcg                                                                                         >  2:749699/1‑151 (MQ=255)
                                                               cAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTgcg                                                                                         <  1:10085092/151‑1 (MQ=255)
                                                                aGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTTGGGTTGATCTGGCCAGGTCGGGGTGCTCGCAAAAGCCCCGCCGTATTgggggggttggg                                                                                        >  1:10837218/1‑145 (MQ=255)
                                                                  gCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTc                                                                                      <  2:5404357/151‑1 (MQ=255)
                                                                  gCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTc                                                                                      <  1:10113488/151‑1 (MQ=255)
                                                                    ccGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGCGGTCGGGTTAATCTGCCCTGTTAGGGCTCCGCGAAAAAGCCCCGCCTTATAGGGCGGGTGCGCTCGa                                                                                    >  2:8906773/1‑151 (MQ=255)
                                                                     cGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGaa                                                                                   <  2:829589/151‑1 (MQ=255)
                                                                      gggTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAAt                                                                                  <  2:4047487/151‑1 (MQ=255)
                                                                       ggTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATg                                                                                 <  1:2777849/151‑1 (MQ=255)
                                                                           aaCCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTGGGTTTAATATGCCATGTTAGGGGTGCGAGAAAAAACCCGGCAGTATAGGGGGGGTTGGGTCGAATTgcgg                                                                             >  1:7059287/1‑150 (MQ=255)
                                                                           aaCCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGGTCGGGGTACACAGCCATGGTGGGGGGCCACGTCAACCCCCCGCAGTATAGGGCGGTTGCGCACGAAAGgcgc                                                                             >  2:9417287/1‑151 (MQ=255)
                                                                            aCCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTAAGTTGGGTTTCATCTGCCATGTTAGGGGTCCTCGAAAAAGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGcgcg                                                                            >  2:1816722/1‑151 (MQ=255)
                                                                               tcctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGcc                                                                         <  2:2614799/151‑1 (MQ=255)
                                                                               tcctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTAAGTTAGGTTTAATATGCCCTTTTAGGGGTGCGAGAAAAAGCCCCGCAATATAGGGCGGTTGCGCTCGAATTGCGCGGcc                                                                         >  1:9878498/1‑151 (MQ=255)
                                                                                cctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTAGGGTTCATCTGCCATGTTAGGGCGCCTCGAAAACGCCCCGCATTATAGGGCGGTTGCGCGCGAATGGCGCGGccc                                                                        >  2:10669336/1‑151 (MQ=255)
                                                                                cctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGCGTTCGGTTTCATAAGCCCTGTTGGGGCTCCGCGAAAACCCCCCGCAGAATAGGGCGGTTGCGCTCGAAATGCGCGGccc                                                                        >  2:5022386/1‑151 (MQ=255)
                                                                                 ctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCg                                                                       <  2:7169124/151‑1 (MQ=255)
                                                                                 ctGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTAGGTTTGATAATCCAAGTTAGGGCTCCTCCAAAACCCCCCGCCGTTTATGGGGGTTGGGCGCGAATTGGGCGGGCCg                                                                       >  1:7817936/1‑151 (MQ=255)
                                                                                  tGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGa                                                                      <  1:10787583/151‑1 (MQ=255)
                                                                                    cACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGACGTGGGTTTGAAATTCCAATTTCGGGGGCCACGAAAAAGCCCCGCCATGTATGGGGGGTGGGCTGGAATGGGGCGGCCCGCTg                                                                    >  1:6799203/1‑151 (MQ=255)
                                                                                      cGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGAGTTCGGGTTCATATGCCCAGTTCGGGCTCCACGAAAACCCCCGGCAGTATAGGGCGGTTGGGCTCGAATGGCGCGGCCCCAAGAc                                                                  >  2:6801313/1‑151 (MQ=255)
                                                                                       gAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACg                                                                 <  2:49282/151‑1 (MQ=255)
                                                                                        aaaGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGa                                                                <  2:1058596/151‑1 (MQ=255)
                                                                                             cACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGt                                                           <  2:9689308/151‑1 (MQ=255)
                                                                                              aCCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTc                                                          <  2:10993310/151‑1 (MQ=255)
                                                                                                 agcagcACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCt                                                       <  1:6851396/151‑1 (MQ=255)
                                                                                                   cagcaCATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTcc                                                     <  1:9707875/151‑1 (MQ=255)
                                                                                                   cagcaCATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTGTCAGTTAGGGTTCAAATGCCCAGGTCGGGCTCCACGAAAACGCCCGGCCTTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGCTGGCGAGGCGGCGCTcc                                                     >  2:2425797/1‑151 (MQ=255)
                                                                                                    agcaCATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTccg                                                    <  1:5963338/151‑1 (MQ=255)
                                                                                                            cAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTc                                            <  1:10006112/151‑1 (MQ=255)
                                                                                                             aGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTGAGTTCGGTTTGATAAGCCAAGTTCGGGCTCCTCGAAAAACCCCCGGATTATAGGGGGGGTTGGCTCGAATGGGGCGGCCCGGTGACGAGGCGGCGCTACGCCGGCGTcc                                           >  2:11175788/1‑151 (MQ=255)
                                                                                                               ttCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTGAGGTCGGGTTCAACTGCCCAGTTCGGGCTCCGCGAAAACCCCCCGCCTTATAGGGCGGTGGGGCTCGAATGGCGCGGCCCCAAGTAGCGGCGTCGCGCCGCCGGCGGCCGc                                         >  2:7892487/1‑151 (MQ=255)
                                                                                                                  cAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCt                                      <  2:5885568/151‑1 (MQ=255)
                                                                                                                   aGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCtt                                     <  1:1200554/151‑1 (MQ=255)
                                                                                                                    gCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTg                                    <  1:2231885/151‑1 (MQ=255)
                                                                                                                     cATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTgg                                   <  2:1510448/151‑1 (MQ=255)
                                                                                                                      aTGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGt                                  <  2:5555308/151‑1 (MQ=255)
                                                                                                                       tGCCGCGACGGCTGTGCCAGTAGAGTCGTGTGGGTTAGGGTTAAACGGGCAGGTTAGGGGACCGAGAAAAAACCCCGCCGTATAGGGCGGGTGGGCGCGGATGGGGCGGGCCGGGGGCCAGGCGGCGGGCCGCCGGCGAGCCCCCATGGTc                                 >  2:3822812/1‑151 (MQ=255)
                                                                                                                          cgcgACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTa                              <  1:9429808/151‑1 (MQ=255)
                                                                                                                          cgcgACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTa                              <  2:1410827/151‑1 (MQ=255)
                                                                                                                           gcgACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTAt                             <  2:8753367/151‑1 (MQ=255)
                                                                                                                           gcgACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCAGGCGTCCGCCCTTGGTCGTAt                             <  1:1379487/151‑1 (MQ=255)
                                                                                                                             gACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTg                           <  1:3566332/151‑1 (MQ=255)
                                                                                                                             gACGGCTGTGCCAGTAGAGTCGTGTGAGTTAGGGTTGATCTGCCAATTTAGGGGGCCACGAAAAACCCCCGCCGGATATGGCGGGTGGGCTCGAAATGGGCGGCCCCAAGAACATGCGGAGCACCCCCCGCGTCCGCCCCAGGGCGTAttt                           >  2:10266717/1‑150 (MQ=255)
                                                                                                                              aCGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGc                          <  1:6269653/151‑1 (MQ=255)
                                                                                                                               cGGCTGTGCCAGTAGAGTCGTGGCAGTTCGGGGTAATATTCCCTGGTAGGGCGCCTAGCAAAAGCCCCGGCGTATAGGGGGGGTGCGCGCCAATGGCGCGGCCCCAAGAAGAGGCGTCGCACCCCCGGCCTCACCCCCGGGTCGGATTTcc                         >  2:2212778/1‑151 (MQ=255)
                                                                                                                                ggCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGccc                        <  2:7264003/151‑1 (MQ=255)
                                                                                                                                 gCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCg                       <  1:4970963/151‑1 (MQ=255)
                                                                                                                                  cTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGCTGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCgg                      <  2:30799/151‑1 (MQ=255)
                                                                                                                                       ccAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAAc                 <  2:4458939/151‑1 (MQ=255)
                                                                                                                                           tAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAg             <  1:9004484/151‑1 (MQ=255)
                                                                                                                                              agTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGt          <  2:5721651/151‑1 (MQ=255)
                                                                                                                                               gTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTa         >  2:902079/1‑151 (MQ=255)
                                                                                                                                                tCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTAt        <  1:6074827/151‑1 (MQ=255)
                                                                                                                                                 cGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTATc       <  2:8112452/151‑1 (MQ=255)
                                                                                                                                                  ctttCATTTCGGTTTCAACTGCCATCTTCGGGCTCCTCGAAAACGCCCGGCATAATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCTTATAGCCCGGCAAACCAAGGGTATcc      >  2:1579128/2‑151 (MQ=255)
                                                                                                                                                   tttCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTATCcg     >  2:6182244/1‑151 (MQ=255)
                                                                                                                                                    ttCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTATCcgc    >  2:2325869/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
ACCGAACATGTCCTGGTCCTCGCACTCCAGCAGCTTGCGGAAGCGCGCCTGGTCCTCGGCGTCCAGGCCCGGGTAAACCTCCTGCACGAAAGGCACCAGCAGCACATCCAGTTCCAGCATGCCGCGACGGCTGTGCCAGTAGAGTCGTTTCAGTTCGGTTTCATCTGCCATGTTCGGGCTCCTCGAAAACGCCCGGCATTATAGGGCGGTTGCGCTCGAATGGCGCGGCCCGATGACGAGGCGTCGCTCCGCCGGCGTCCGCCCTTGGTCGTATTGCCCGGCAAACCAAGGGTATCCGCGC  >  NC_008463/4829167‑4829467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑