Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,198,441 T→G A547A (GCA→GCC)  PA14_RS14615 ← acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,198,4410TG99.8% 1708.9 / NA 431A547A (GCA→GCC) PA14_RS14615acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (242/188);  total (242/189)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

CTCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAGA  >  NC_008463/3198293‑3198588
                                                                                                                                                    |                                                                                                                                                   
cTCAGGGTGAAACGGTGTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCGAGGTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGcggccggc                                                                                                                                                   >  1:10246347/1‑151 (MQ=255)
    gggTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCg                                                                                                                                               >  2:5585830/1‑151 (MQ=255)
     ggTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGACTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGc                                                                                                                                              >  2:6009277/1‑151 (MQ=255)
       tGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCg                                                                                                                                            >  1:9469064/1‑151 (MQ=255)
        gAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCgg                                                                                                                                           >  2:10020379/1‑151 (MQ=255)
          aaCGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcc                                                                                                                                         <  1:1960647/151‑1 (MQ=255)
              gTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcct                                                                                                                                     >  2:5070676/1‑151 (MQ=255)
                    gcgcgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGcctcctcctcct                                                                                                                               <  2:10420943/151‑1 (MQ=255)
                     cgcgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTg                                                                                                                              >  1:10962812/1‑151 (MQ=255)
                       cgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGcc                                                                                                                            >  1:3443343/1‑151 (MQ=255)
                       cgcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGcc                                                                                                                            <  1:1752221/151‑1 (MQ=255)
                        gcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCg                                                                                                                           >  1:7634368/1‑151 (MQ=255)
                        gcCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCg                                                                                                                           >  1:1499422/1‑151 (MQ=255)
                                ttGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACt                                                                                                                   >  1:1243185/1‑151 (MQ=255)
                                ttGCTGGAACCGGGCACTGGCGCGGAGACGTGCGTATAGCAGGCTGGCCATGGGACACCTCGGGCCACCTAGTTAGTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACt                                                                                                                   <  2:4824699/151‑1 (MQ=255)
                                 tGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCTACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTc                                                                                                                  <  1:4301443/151‑1 (MQ=255)
                                 tGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTc                                                                                                                  <  1:9601684/151‑1 (MQ=255)
                                  gCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcg                                                                                                                 >  2:9557296/1‑151 (MQ=255)
                                    tGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcgcg                                                                                                               <  1:8551791/151‑1 (MQ=255)
                                    tGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTcgcg                                                                                                               >  2:4372047/1‑151 (MQ=255)
                                       aaCCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAg                                                                                                            >  2:1490978/1‑151 (MQ=255)
                                          cGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                         <  2:6530626/151‑1 (MQ=255)
                                          cGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                         >  1:6905581/1‑151 (MQ=255)
                                          cGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGAc                                                                                                         <  1:4812152/151‑1 (MQ=255)
                                            ggCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGa                                                                                                       <  2:196295/151‑1 (MQ=255)
                                               aCTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACt                                                                                                    >  1:4414888/1‑151 (MQ=255)
                                                cTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACtt                                                                                                   >  2:295053/1‑151 (MQ=255)
                                                   gcgcGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTg                                                                                                <  2:6658631/151‑1 (MQ=255)
                                                            cGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGcc                                                                                       <  1:8877667/151‑1 (MQ=255)
                                                             gTGCGTAGAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCg                                                                                      <  2:206438/151‑1 (MQ=255)
                                                              tGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCgg                                                                                     >  1:10440260/1‑151 (MQ=255)
                                                              tGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCgg                                                                                     <  2:1527522/151‑1 (MQ=255)
                                                                  tataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGc                                                                                 <  2:2415663/151‑1 (MQ=255)
                                                                   ataGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCg                                                                                <  1:1690731/151‑1 (MQ=255)
                                                                    taGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCgg                                                                               >  2:3064604/1‑151 (MQ=255)
                                                                     aGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGt                                                                              <  1:8818607/151‑1 (MQ=255)
                                                                     aGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGt                                                                              >  1:2491826/1‑151 (MQ=255)
                                                                      gCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACTAACTTCTGGATCTTGCCGGTGGCGGTc                                                                             >  2:10554433/1‑151 (MQ=255)
                                                                       cAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTcg                                                                            >  1:9400277/1‑150 (MQ=255)
                                                                         ggCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTg                                                                          <  2:6248040/151‑1 (MQ=255)
                                                                           cTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTggg                                                                        >  1:6690805/1‑151 (MQ=255)
                                                                           cTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTggg                                                                        <  2:1589175/151‑1 (MQ=255)
                                                                           cTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACATCTGGATCTTGCCGGTGGCGGTCTTggg                                                                        >  1:100162/1‑151 (MQ=255)
                                                                            tGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGc                                                                       >  2:3403162/1‑151 (MQ=255)
                                                                            tGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTACTGGATCTTGCCGGAGGCGGTCTTGGGc                                                                       >  2:274085/1‑151 (MQ=255)
                                                                             ggACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGGCTTGGGCa                                                                      >  1:5960389/1‑151 (MQ=255)
                                                                               aCATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCGTGGGCAGt                                                                    >  2:552740/1‑151 (MQ=255)
                                                                                  tGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCg                                                                 >  2:9585074/1‑151 (MQ=255)
                                                                                   gggACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCgg                                                                <  1:275541/151‑1 (MQ=255)
                                                                                   gggACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCgg                                                                <  1:4129229/151‑1 (MQ=255)
                                                                                    ggACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGt                                                               >  2:6463008/1‑151 (MQ=255)
                                                                                    ggACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGt                                                               <  2:8416288/151‑1 (MQ=255)
                                                                                     gACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTa                                                              <  1:6372162/151‑1 (MQ=255)
                                                                                     gACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTa                                                              >  1:4473330/1‑151 (MQ=255)
                                                                                      acacCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGACGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGAGCGTGCCGGTGGCGGTCTTGGGCAGTTCGGTaa                                                             >  2:4537647/1‑151 (MQ=255)
                                                                                         ccTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCg                                                          <  2:8003274/151‑1 (MQ=255)
                                                                                         ccTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCg                                                          >  1:7349226/1‑151 (MQ=255)
                                                                                            cGGGCAACCTAGTTATTGTTCTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTca                                                       <  2:10366534/151‑1 (MQ=255)
                                                                                            cGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTca                                                       >  1:5502714/1‑151 (MQ=255)
                                                                                               gCAACCTGGGTATGGGTATGGAATGTGCGGGTGCGGCAGCGCCGTCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGTTCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTCAGCGTCACAt                                                    >  2:6978161/1‑151 (MQ=255)
                                                                                               gCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCTCTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACAt                                                    <  1:2588533/151‑1 (MQ=255)
                                                                                               gCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACAt                                                    <  1:5149023/151‑1 (MQ=255)
                                                                                                cAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATg                                                   <  1:6276452/151‑1 (MQ=255)
                                                                                                  aCCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGcc                                                 <  1:4017716/151‑1 (MQ=255)
                                                                                                   ccTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCcg                                                <  2:10148150/151‑1 (MQ=255)
                                                                                                    cTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCcgc                                               >  2:8073735/1‑151 (MQ=255)
                                                                                                     tAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCcgcg                                              <  2:1106154/151‑1 (MQ=255)
                                                                                                        ttATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTAGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCgcggcg                                           >  2:8935054/1‑151 (MQ=255)
                                                                                                         tATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGgcgc                                          <  1:3806974/151‑1 (MQ=255)
                                                                                                          atggTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGACCTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGcc                                         >  2:1709125/4‑151 (MQ=255)
                                                                                                          aTTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGcc                                         <  1:9729584/151‑1 (MQ=255)
                                                                                                           ttGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCt                                        >  1:8269523/1‑151 (MQ=255)
                                                                                                            tGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCtt                                       <  1:9165632/151‑1 (MQ=255)
                                                                                                              ttATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGa                                     <  1:4921411/151‑1 (MQ=255)
                                                                                                              ttATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGa                                     <  2:6748603/151‑1 (MQ=255)
                                                                                                                aTGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAg                                   <  1:11154532/151‑1 (MQ=255)
                                                                                                                 tGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGt                                  >  2:3169260/1‑151 (MQ=255)
                                                                                                                  ggAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTg                                 >  1:2961856/1‑151 (MQ=255)
                                                                                                                  ggAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTg                                 >  2:3865665/1‑151 (MQ=255)
                                                                                                                   gAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTgc                                >  1:8109495/1‑151 (MQ=255)
                                                                                                                    aaTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTgcg                               <  1:10908870/151‑1 (MQ=255)
                                                                                                                    aaTGTGCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTgcg                               >  1:5414872/1‑151 (MQ=255)
                                                                                                                      tgtgCGGGTGCGGCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGcc                             <  2:3504667/151‑1 (MQ=255)
                                                                                                                        tgcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGCTCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAg                           <  1:7003905/151‑1 (MQ=255)
                                                                                                                        tgcgggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAg                           <  2:7316717/151‑1 (MQ=255)
                                                                                                                           gggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGgcg                        <  1:5309480/151‑1 (MQ=255)
                                                                                                                           gggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGgcg                        >  2:9107634/1‑151 (MQ=255)
                                                                                                                           gggtgcggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACGTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGgcg                        <  2:3299831/151‑1 (MQ=255)
                                                                                                                                 ggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTcgcg                  <  1:324483/151‑1 (MQ=255)
                                                                                                                                 ggCAGCGCCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTcgcg                  >  1:10884867/1‑151 (MQ=255)
                                                                                                                                  gCAGCGCCTTCAGCGGCAGGCGTCGCCGGCCTCCTCCGCCTGCCGGGACCACTCGCGCAGGACGAACGTCTGGTTCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTACGCGTCACATGACGCTGCGCCTTGAAGTGCGCCAGGCGCTagctg                 <  2:6348168/151‑3 (MQ=255)
                                                                                                                                     gcgcCATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCAc              >  2:1413144/1‑151 (MQ=255)
                                                                                                                                        ccATCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAg           >  2:1139779/1‑151 (MQ=255)
                                                                                                                                           tCAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCgg        >  1:9908565/1‑151 (MQ=255)
                                                                                                                                            cAGCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGa       >  2:7544651/1‑151 (MQ=255)
                                                                                                                                              gCGGCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGAcc     >  1:3685496/1‑151 (MQ=255)
                                                                                                                                               cggccggcGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCa    >  2:5382516/1‑151 (MQ=255)
                                                                                                                                                ggCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAg   >  1:10132025/1‑151 (MQ=255)
                                                                                                                                                ggCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTGCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAg   >  2:3954328/1‑151 (MQ=255)
                                                                                                                                                 gCCGGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAGa  <  2:4651908/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTCAGGGTGAAACGGTTTCGGCGCGCCACCAGTTGCTGGAACCGGGCACTGGCGCGGATACGTGCGTATAGCAGGCTGGACATGGGACACCTCGGGCAACCTAGTTATTGTTATGGAATGTGCGGGTGCGGCAGCGCCATCAGCGGCCTGCGTCGCCGGCCTCCTCCTCCTGCCGGGCCCACTCGCGCAGGACGAACTTCTGGATCTTGCCGGTGGCGGTCTTGGGCAGTTCGGTAAGCGTCACATGCCGCGGCGCCTTGAAGTGCGCCAGGCGCTCGCGGCACCAGCGGACCAGA  >  NC_008463/3198293‑3198588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑