Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,373,667 G→C V352V (GTC→GTG)  PA14_RS15385 ← aminotransferase class V‑fold PLP‑dependent enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,373,6670GC100.0% 1888.0 / NA 475V352V (GTC→GTG) PA14_RS15385aminotransferase class V‑fold PLP‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (252/223);  total (252/223)

Only 100 of 477 total aligned reads displayed.

GAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGTC  >  NC_008463/3373519‑3373814
                                                                                                                                                    |                                                                                                                                                   
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   <  1:10047434/151‑1 (MQ=255)
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   >  1:4259521/1‑151 (MQ=255)
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   <  2:10447865/151‑1 (MQ=255)
gAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCAc                                                                                                                                                   >  2:966214/1‑151 (MQ=255)
gAGGCCGGGACGAGCCTGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACTGCAGCCACGCCTGCGCGCCAAAAACCGCCGCGGCCTTGATGGCGCCCACCGCAGCGACGCCGTCGCCCACGCGGGAGTAGCGACCCAGCCACACGCGCAc                                                                                                                                                   >  2:2766031/1‑151 (MQ=255)
  gggCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGCTACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTCTCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGGAGCGACCGAGGCTCAGGCGCACGc                                                                                                                                                 >  1:561105/1‑151 (MQ=255)
  gggCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGc                                                                                                                                                 >  1:7522221/1‑151 (MQ=255)
     cGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGc                                                                                                                                              <  1:7409345/151‑1 (MQ=255)
      gggTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCg                                                                                                                                             >  1:4576783/1‑151 (MQ=255)
       ggTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCgg                                                                                                                                            >  2:6409928/1‑151 (MQ=255)
            aGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAgc                                                                                                                                       <  1:10745478/151‑1 (MQ=255)
             gccggccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAgcg                                                                                                                                      <  2:7107685/151‑1 (MQ=255)
                 gccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTgg                                                                                                                                  <  2:530024/151‑1 (MQ=255)
                 gccgCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTgg                                                                                                                                  <  1:9306203/151‑1 (MQ=255)
                     cTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTggcggc                                                                                                                              >  1:9756167/1‑151 (MQ=255)
                      tCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCTCCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCa                                                                                                                             <  2:1873390/151‑1 (MQ=255)
                      tCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCa                                                                                                                             >  1:10149872/1‑151 (MQ=255)
                       cAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCAt                                                                                                                            <  1:3473983/151‑1 (MQ=255)
                           cTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTg                                                                                                                        <  2:4333624/151‑1 (MQ=255)
                           cTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTg                                                                                                                        >  1:5810317/1‑151 (MQ=255)
                              cccTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCGTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGcc                                                                                                                     >  1:7930314/1‑151 (MQ=255)
                               ccTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTCTCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCg                                                                                                                    <  2:5651459/151‑1 (MQ=255)
                                cTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCGTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCTCGCTGCGGCCAGCGCTGGCGGCAGCGTGGCCGa                                                                                                                   >  1:8419442/1‑151 (MQ=255)
                                 tGGATACCCCGAGGACCGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTGGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAg                                                                                                                  >  2:3102917/1‑151 (MQ=255)
                                   gATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAggg                                                                                                                >  1:3576408/1‑151 (MQ=255)
                                     tACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCg                                                                                                              <  2:3465453/151‑1 (MQ=255)
                                      aCCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCga                                                                                                             >  2:10644674/1‑151 (MQ=255)
                                      aCCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCga                                                                                                             >  2:7413108/1‑151 (MQ=255)
                                             ggAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCAc                                                                                                      <  2:6881932/151‑1 (MQ=255)
                                                 cGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTgg                                                                                                  <  1:8822952/151‑1 (MQ=255)
                                                  ggCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTggg                                                                                                 >  1:2708935/1‑151 (MQ=255)
                                                   gCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTGGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGTGGGCGAGTAGCACGAGGGa                                                                                                >  2:6630241/1‑151 (MQ=255)
                                                    cTGCCNCGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCCGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGGGGGAc                                                                                               >  1:9567518/1‑151 (MQ=255)
                                                         aCGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGgcgc                                                                                          <  2:9590782/151‑1 (MQ=255)
                                                           gCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccg                                                                                        >  2:10890274/1‑151 (MQ=255)
                                                           gCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccg                                                                                        <  1:7121723/151‑1 (MQ=255)
                                                            ccGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccgg                                                                                       >  1:3357361/1‑151 (MQ=255)
                                                             cGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGGGGGACGGCgccggc                                                                                      >  1:4857174/1‑151 (MQ=255)
                                                               gcgcgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCgccggccg                                                                                    <  2:1973918/151‑1 (MQ=255)
                                                                 gcgcCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCcgcg                                                                                  <  1:414333/151‑1 (MQ=255)
                                                                     cACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGCCGGCGCCGGCCGCGCCGa                                                                              >  1:616016/1‑151 (MQ=255)
                                                                     cACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGa                                                                              >  2:8118719/1‑151 (MQ=255)
                                                                      aCTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAg                                                                             <  2:2967348/151‑1 (MQ=255)
                                                                      aCTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAg                                                                             <  1:10922475/151‑1 (MQ=255)
                                                                       cTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGt                                                                            >  2:11003036/1‑151 (MQ=255)
                                                                        tATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGtt                                                                           <  1:120250/151‑1 (MQ=255)
                                                                           ccgccgCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCa                                                                        >  1:4050450/1‑151 (MQ=255)
                                                                           ccgccgCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCa                                                                        >  1:2424990/1‑151 (MQ=255)
                                                                            cgccgcGGCCTTGCGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAg                                                                       <  2:8193102/151‑1 (MQ=255)
                                                                            cgccgcGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAg                                                                       >  2:4748086/1‑151 (MQ=255)
                                                                             gccgcGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAgg                                                                      >  2:404112/1‑151 (MQ=255)
                                                                                  ggCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAg                                                                 <  1:3381859/151‑1 (MQ=255)
                                                                                  ggCCGTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGCGGTGCAGGCCGAg                                                                 >  1:7007963/1‑151 (MQ=255)
                                                                                    ccTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGt                                                               <  2:1471559/151‑1 (MQ=255)
                                                                                     cTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTg                                                              >  1:10848810/1‑151 (MQ=255)
                                                                                     cTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTg                                                              <  2:10282412/151‑1 (MQ=255)
                                                                                          gggCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGa                                                         <  1:1496694/151‑1 (MQ=255)
                                                                                          gggCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGa                                                         <  2:232690/151‑1 (MQ=255)
                                                                                           ggcggcCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGac                                                        <  1:3912937/151‑1 (MQ=255)
                                                                                            gcggcCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGaca                                                       >  2:5879752/1‑151 (MQ=255)
                                                                                               gcCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCg                                                    <  2:4426220/151‑1 (MQ=255)
                                                                                                ccACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGc                                                   <  1:5964895/151‑1 (MQ=255)
                                                                                                 cACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGcc                                                  >  1:10545421/1‑151 (MQ=255)
                                                                                                    cgccgcGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAgg                                               >  1:9647886/1‑151 (MQ=255)
                                                                                                         cgTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGa                                          <  1:9830977/151‑1 (MQ=255)
                                                                                                          gTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGAc                                         >  1:6072609/1‑151 (MQ=255)
                                                                                                          gTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGAc                                         <  2:7632757/151‑1 (MQ=255)
                                                                                                          gTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGAc                                         <  2:10100097/151‑1 (MQ=255)
                                                                                                             gACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTcc                                      >  2:659053/1‑151 (MQ=255)
                                                                                                             gACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTcc                                      >  1:7735953/1‑151 (MQ=255)
                                                                                                             gACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTcc                                      >  2:2554242/1‑151 (MQ=255)
                                                                                                             gACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTcc                                      >  2:896954/1‑151 (MQ=255)
                                                                                                               cGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCTGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAg                                    >  2:9265967/1‑151 (MQ=255)
                                                                                                               cGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAg                                    >  2:8704998/1‑151 (MQ=255)
                                                                                                                gTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGt                                   >  2:1146409/1‑151 (MQ=255)
                                                                                                                gTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTAGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGt                                   >  2:8348846/1‑151 (MQ=255)
                                                                                                                    gCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCt                               <  1:8600258/151‑1 (MQ=255)
                                                                                                                     ccTCGCGGGTGTAGCGACCTAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTg                              <  1:6841573/151‑1 (MQ=255)
                                                                                                                     ccTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTg                              >  2:6177606/1‑151 (MQ=255)
                                                                                                                      cTCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgg                             >  2:5472325/1‑151 (MQ=255)
                                                                                                                       tCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggg                            <  1:9995086/151‑1 (MQ=255)
                                                                                                                       tCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggg                            >  2:5028060/1‑151 (MQ=255)
                                                                                                                       tCGCGGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTggg                            <  1:2795386/151‑1 (MQ=255)
                                                                                                                        cgcgGGTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTgggg                           >  2:5998055/1‑151 (MQ=255)
                                                                                                                           gggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGAc                        >  1:9174810/1‑151 (MQ=255)
                                                                                                                           gggTGTAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGAc                        >  2:3701483/1‑151 (MQ=255)
                                                                                                                              tgtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACaaa                     <  1:8226298/151‑1 (MQ=255)
                                                                                                                              tgtAGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACaaa                     >  1:1537292/1‑151 (MQ=255)
                                                                                                                                 aGCGACCGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGc                  <  1:9027792/151‑1 (MQ=255)
                                                                                                                                      ccGAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGt             <  2:4141677/151‑1 (MQ=255)
                                                                                                                                        gAGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTg           <  2:3250709/151‑1 (MQ=255)
                                                                                                                                         aGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGt          <  1:6250208/151‑1 (MQ=255)
                                                                                                                                         aGGCTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGt          <  2:3877490/151‑1 (MQ=255)
                                                                                                                                            cTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGa       >  1:6308436/1‑151 (MQ=255)
                                                                                                                                            cTCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACACCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGa       >  2:8862798/1‑151 (MQ=255)
                                                                                                                                             tCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGaa      <  2:7548702/151‑1 (MQ=255)
                                                                                                                                             tCAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGaa      <  1:1828050/151‑1 (MQ=255)
                                                                                                                                              cAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAg     <  1:2070420/151‑1 (MQ=255)
                                                                                                                                              cAGGCGCACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAg     >  2:10475407/1‑151 (MQ=255)
                                                                                                                                                ggCGCACGCTGCGGCCTGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCTCGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGTCACCGACAGGCTGGACTCCAGTGCCTGGGGGACAAAGTCCGGGTTGTCGAAGGt   <  1:2146125/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGGGCGGGTCGAGCCGGCCGCTCAGCCTGCCCTGGATACCCCGAGGAACGGCTGCCACGCCGGCGCGCCACTATCCGCCGCGGCCTTGAGGGCGGCCACCGCCGCGTCGACGTCGGCCTCGCGGGTGTAGCGACCGAGGCTCAGGCGGACGCTGCGGCCAGCGCTGGCGGCATCGTGGCCGAGGGCGAGAAGCACGTGGGACGGCGCCGGCCGCGCCGAGTTGCAGGCCGAGGTGCTGGACACCGCCAGGCTGGACTCCAGTGCCTGGGGGACAAAGGCCGGGTTGTCGAAGGTC  >  NC_008463/3373519‑3373814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑