Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,769,180 C→G A44A (GCG→GCC)  pscL ← SctL family type III secretion system stator protein PscL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,769,1800CG100.0% 1942.2 / NA 497A44A (GCG→GCC) pscLSctL family type III secretion system stator protein PscL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (259/238);  total (259/238)

Only 100 of 510 total aligned reads displayed.

GGCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGTC  >  NC_008463/3769032‑3769327
                                                                                                                                                    |                                                                                                                                                   
      cGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACg                                                                                                                                             <  2:7658906/151‑1 (MQ=255)
        aCCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCt                                                                                                                                           <  2:10456740/151‑1 (MQ=255)
         ccTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTc                                                                                                                                          <  2:3328007/151‑1 (MQ=255)
          cTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTcg                                                                                                                                         >  2:10056933/1‑151 (MQ=255)
           tGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTcgc                                                                                                                                        <  2:5084541/151‑1 (MQ=255)
            gCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTcgcg                                                                                                                                       <  1:9565786/151‑1 (MQ=255)
             ccGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGt                                                                                                                                      <  1:8704757/151‑1 (MQ=255)
              cGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTg                                                                                                                                     >  1:2695241/1‑151 (MQ=255)
               ggTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGTCGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCGCGTGGGCCTCGCGCTCGCTTTCGGCGGCTAGCTCGCGTGc                                                                                                                                    >  2:3887928/1‑151 (MQ=255)
                  agtagCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGc                                                                                                                                 >  2:1295150/1‑151 (MQ=255)
                       cGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTcca                                                                                                                            >  2:2245905/1‑151 (MQ=255)
                       cGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCACGATTTCGGCGGCACGCTCGCGTGCCGCTTcca                                                                                                                            <  2:4763282/151‑1 (MQ=255)
                           tGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAg                                                                                                                        <  2:5615804/151‑1 (MQ=255)
                           tGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAg                                                                                                                        <  2:11099303/151‑1 (MQ=255)
                            gCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAgg                                                                                                                       >  2:5400659/1‑151 (MQ=255)
                             cAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGc                                                                                                                      <  2:6072919/151‑1 (MQ=255)
                                    gTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgc                                                                                                               <  2:6158825/151‑1 (MQ=255)
                                      aGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgcgc                                                                                                             >  1:10491207/1‑151 (MQ=255)
                                      aGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCGTCCACCAGGCGGTTGgcgc                                                                                                             >  2:8595308/1‑151 (MQ=255)
                                          tttCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAg                                                                                                         >  2:1739565/1‑151 (MQ=255)
                                          tttCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAg                                                                                                         >  1:11105033/1‑151 (MQ=255)
                                           ttCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAATCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCTCGCTCGCGTGCCGCTTCCACCAGGCGGGTGGCGCTGAgg                                                                                                        >  2:7000477/1‑151 (MQ=255)
                                             ccTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTa                                                                                                      >  2:6658151/1‑151 (MQ=255)
                                             ccTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTa                                                                                                      <  1:10437964/151‑1 (MQ=255)
                                                 gATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTcc                                                                                                  >  1:3638965/1‑151 (MQ=255)
                                                 gATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTcc                                                                                                  <  1:1241604/151‑1 (MQ=255)
                                                   tCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTg                                                                                                >  2:9367448/1‑151 (MQ=255)
                                                   tCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGGAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTccgg                                                                                                >  2:1822213/1‑149 (MQ=255)
                                                    cAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCGTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTgg                                                                                               >  2:9872085/1‑151 (MQ=255)
                                                     aGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGt                                                                                              >  2:9212242/1‑151 (MQ=255)
                                                     aGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGt                                                                                              >  2:4635071/1‑151 (MQ=255)
                                                     aGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCGCGCGTGCCGCTTCCTCCAGGCGGTTGGCGCTGAGGTAGTCCTGGt                                                                                              >  1:1493473/1‑151 (MQ=255)
                                                       cccGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAg                                                                                            <  2:892533/151‑1 (MQ=255)
                                                        ccGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGt                                                                                           >  2:10989117/1‑151 (MQ=255)
                                                        ccGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGt                                                                                           <  1:4177750/151‑1 (MQ=255)
                                                         cGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTc                                                                                          <  1:925603/151‑1 (MQ=255)
                                                         cGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTc                                                                                          >  1:9812212/1‑151 (MQ=255)
                                                            ccTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTcgcg                                                                                       >  2:3639930/1‑151 (MQ=255)
                                                              tGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGTGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCggg                                                                                     >  1:6163884/1‑151 (MQ=255)
                                                               ggCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGgc                                                                                    >  1:5498920/1‑151 (MQ=255)
                                                                   cAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGgcgcgc                                                                                >  2:3531978/1‑151 (MQ=255)
                                                                          gcCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAggg                                                                         <  2:7463490/151‑1 (MQ=255)
                                                                            ctcctcCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGcc                                                                       <  1:5643357/151‑1 (MQ=255)
                                                                             tcctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCTCGCTCGCGTGCCGCTTCCTCCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCt                                                                      >  2:8286262/1‑151 (MQ=255)
                                                                              cctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCGCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTg                                                                     >  2:5877190/1‑151 (MQ=255)
                                                                               ctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGc                                                                    <  2:4403059/151‑1 (MQ=255)
                                                                                   cgTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgg                                                                <  2:7361824/150‑1 (MQ=255)
                                                                                    gTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCggg                                                               <  2:7792374/151‑1 (MQ=255)
                                                                                     tCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgggg                                                              >  1:8543789/1‑151 (MQ=255)
                                                                                     tCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgggg                                                              <  2:2637840/151‑1 (MQ=255)
                                                                                       cGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGc                                                            <  1:1862848/151‑1 (MQ=255)
                                                                                         gCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGTCCa                                                          <  2:4036086/151‑1 (MQ=255)
                                                                                          ccTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAg                                                         >  2:4285550/1‑151 (MQ=255)
                                                                                          ccTCCCAGCCCAGGCGCTACTGCTCCTGGTAACCCACGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGATGTAGTCCGGGTTGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAg                                                         <  1:479696/151‑1 (MQ=255)
                                                                                           cTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCGGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGc                                                        >  1:7977143/1‑151 (MQ=255)
                                                                                             cccagcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGGGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCg                                                      <  2:8726982/151‑1 (MQ=255)
                                                                                              ccagcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGt                                                     <  1:1704304/151‑1 (MQ=255)
                                                                                                agcccagGCGCTTCTGCTCCTGGTAAACCTGGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAc                                                   <  2:6470855/151‑1 (MQ=255)
                                                                                                agcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAc                                                   >  1:3626526/1‑151 (MQ=255)
                                                                                                agcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAc                                                   <  2:10671010/151‑1 (MQ=255)
                                                                                                agcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAc                                                   >  1:6518917/1‑151 (MQ=255)
                                                                                                agcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCGTCCACCAGGCGGTTGGCGCGGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAc                                                   >  1:7680852/1‑151 (MQ=255)
                                                                                                   ccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgcg                                                >  1:5924436/1‑151 (MQ=255)
                                                                                                    cagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGa                                               >  1:3825898/1‑151 (MQ=255)
                                                                                                    cagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGa                                               >  2:4990399/1‑151 (MQ=255)
                                                                                                       gcgcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTg                                            >  2:1172166/1‑151 (MQ=255)
                                                                                                       gcgcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTg                                            <  1:6268000/151‑1 (MQ=255)
                                                                                                        cgcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTgg                                           >  1:10153402/1‑151 (MQ=255)
                                                                                                         gcatCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCTGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACGGGc                                          >  2:4864365/4‑151 (MQ=255)
                                                                                                         gcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGc                                          >  1:8313780/1‑151 (MQ=255)
                                                                                                         gcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTAGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGc                                          >  2:3411232/1‑151 (MQ=255)
                                                                                                           ttCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGt                                        <  1:4290595/151‑1 (MQ=255)
                                                                                                              tGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTa                                     >  1:703680/1‑151 (MQ=255)
                                                                                                              tGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTa                                     <  2:5703540/151‑1 (MQ=255)
                                                                                                               gCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAg                                    >  1:3323810/1‑151 (MQ=255)
                                                                                                                cTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGt                                   <  1:2173702/151‑1 (MQ=255)
                                                                                                                 tCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGtt                                  <  1:6328229/151‑1 (MQ=255)
                                                                                                                  ccTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTc                                 <  2:1616426/151‑1 (MQ=255)
                                                                                                                   cTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTca                                <  2:2958054/151‑1 (MQ=255)
                                                                                                                    tGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTcaa                               <  2:10578550/151‑1 (MQ=255)
                                                                                                                     ggTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTcaac                              <  2:6279724/151‑1 (MQ=255)
                                                                                                                       tAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTcaacaa                            <  1:7248584/151‑1 (MQ=255)
                                                                                                                          aCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATg                         >  1:7538030/1‑151 (MQ=255)
                                                                                                                          aCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATg                         >  1:4995487/1‑151 (MQ=255)
                                                                                                                          aCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATg                         >  1:2376852/1‑151 (MQ=255)
                                                                                                                           ccTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGTGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATgg                        <  2:5267728/151‑1 (MQ=255)
                                                                                                                           ccTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATgg                        >  1:4545806/1‑151 (MQ=255)
                                                                                                                            cTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGa                       <  1:10042727/151‑1 (MQ=255)
                                                                                                                            cTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGa                       >  1:7783644/1‑151 (MQ=255)
                                                                                                                                tGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCa                   >  2:10224874/1‑151 (MQ=255)
                                                                                                                                    ccTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCa               >  1:2499940/1‑151 (MQ=255)
                                                                                                                                    ccTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCa               <  2:9578837/151‑1 (MQ=255)
                                                                                                                                      tCGCGCTCGCTGTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtc             <  2:6414806/151‑1 (MQ=255)
                                                                                                                                      tCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtc             <  2:687477/151‑1 (MQ=255)
                                                                                                                                       cgcgCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcg            <  1:10720283/151‑1 (MQ=255)
                                                                                                                                           cTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctcg        >  1:6760835/1‑151 (MQ=255)
                                                                                                                                            tCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctcgc       >  1:2879711/1‑151 (MQ=255)
                                                                                                                                              gATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCtt     >  2:8482133/1‑151 (MQ=255)
                                                                                                                                                 ttCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGTc  <  2:8036929/151‑1 (MQ=255)
                                                                                                                                                 ttCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGTc  >  1:10964029/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGTC  >  NC_008463/3769032‑3769327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑