Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,947,683 A→G H166R (CAC→CGC)  PA14_RS22670 → DNA polymerase Y family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,947,6830AG100.0% 1464.2 / NA 374H166R (CAC→CGC) PA14_RS22670DNA polymerase Y family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (194/180);  total (194/180)

Only 100 of 374 total aligned reads displayed.

GGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGTT  >  NC_008463/4947536‑4947830
                                                                                                                                                    |                                                                                                                                                   
 gCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAg                                                                                                                                                 <  1:9541768/151‑1 (MQ=255)
  ccACGCTTCGAGGCCCGCTTGCGCACGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGCCAGCGCTGCAACGCCAGt                                                                                                                                                >  2:1034602/1‑151 (MQ=255)
   cACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGtt                                                                                                                                               <  1:3730980/151‑1 (MQ=255)
    aCGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTg                                                                                                                                              <  1:213106/151‑1 (MQ=255)
      gCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTc                                                                                                                                            >  1:2791011/1‑151 (MQ=255)
        ttCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCg                                                                                                                                          <  1:10820872/151‑1 (MQ=255)
         tCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGc                                                                                                                                         >  1:5812345/1‑151 (MQ=255)
          cGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGcc                                                                                                                                        >  2:7732068/1‑151 (MQ=255)
           gAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGccc                                                                                                                                       <  1:10977448/151‑1 (MQ=255)
            aGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCt                                                                                                                                      >  2:10581762/1‑151 (MQ=255)
             ggCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                                                                                                     <  1:3589849/151‑1 (MQ=255)
             ggCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                                                                                                     >  2:5656833/1‑151 (MQ=255)
                ccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCg                                                                                                                                  >  1:7390617/1‑151 (MQ=255)
                ccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCg                                                                                                                                  >  2:7250575/1‑151 (MQ=255)
                  gCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCAACGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTACCGCt                                                                                                                                >  1:4881018/1‑151 (MQ=255)
                   cTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTg                                                                                                                               >  2:9411617/1‑151 (MQ=255)
                    ttGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTgg                                                                                                                              <  2:7159795/151‑1 (MQ=255)
                           aGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCt                                                                                                                       <  2:1413357/151‑1 (MQ=255)
                           aGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGACTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCt                                                                                                                       >  2:3116674/1‑151 (MQ=255)
                           aGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGACTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCt                                                                                                                       >  2:8749252/1‑151 (MQ=255)
                            ggAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTg                                                                                                                      >  1:6543710/1‑151 (MQ=255)
                               aCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                                                                                                   <  1:8873953/151‑1 (MQ=255)
                               aCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                                                                                                   >  2:4019455/1‑151 (MQ=255)
                                cTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                                                                                                  >  1:4615342/1‑151 (MQ=255)
                                cTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                                                                                                  >  1:6265217/1‑151 (MQ=255)
                                     ggCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcg                                                                                                             >  2:10157295/1‑151 (MQ=255)
                                      gcgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgc                                                                                                            <  2:4460035/151‑1 (MQ=255)
                                        gcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCgg                                                                                                          <  2:9782853/151‑1 (MQ=255)
                                        gcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCgg                                                                                                          <  2:6051854/151‑1 (MQ=255)
                                         cTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCggg                                                                                                         >  1:1617551/1‑151 (MQ=255)
                                          tGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGa                                                                                                        >  1:3874383/1‑151 (MQ=255)
                                            ggCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGc                                                                                                      <  2:11214543/151‑1 (MQ=255)
                                             gCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCt                                                                                                     <  2:4272066/151‑1 (MQ=255)
                                               ttCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTgg                                                                                                   <  2:9051919/151‑1 (MQ=255)
                                                tCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGc                                                                                                  >  1:1925990/1‑151 (MQ=255)
                                                 ccGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGcc                                                                                                 <  2:7542284/151‑1 (MQ=255)
                                                   gCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                                                                                               <  1:6956741/151‑1 (MQ=255)
                                                   gCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                                                                                               <  1:1275877/151‑1 (MQ=255)
                                                    ccctCGCCTGTGCCTGGCGCCGTACCCGGTGGCGGCGCGGTTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAAAGAGCGTTGTCCGCCCTTCCGCGGGACCGCCTGGGAATCTCGCGGGGGCTGGCCGTc                                                                                              >  2:10741573/4‑151 (MQ=255)
                                                     cATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                                                                                             <  2:3449185/151‑1 (MQ=255)
                                                     cATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                                                                                             <  1:8641420/151‑1 (MQ=255)
                                                       tCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGcc                                                                                           <  1:3094752/151‑1 (MQ=255)
                                                        cGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGccc                                                                                          >  1:10206824/1‑151 (MQ=255)
                                                         gCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                                                                                         >  1:6392693/1‑151 (MQ=255)
                                                         gCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                                                                                         <  2:2843996/151‑1 (MQ=255)
                                                          ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTc                                                                                        >  2:10676081/1‑151 (MQ=255)
                                                            tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCgg                                                                                      <  2:8205912/151‑1 (MQ=255)
                                                            tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCgg                                                                                      <  2:460798/151‑1 (MQ=255)
                                                            tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCgg                                                                                      >  1:3179333/1‑151 (MQ=255)
                                                             gtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGa                                                                                     >  2:2072038/1‑151 (MQ=255)
                                                              tgCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGAc                                                                                    <  2:6611468/151‑1 (MQ=255)
                                                                ccTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGc                                                                                  <  2:4161522/151‑1 (MQ=255)
                                                                ccTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGc                                                                                  <  2:4163153/151‑1 (MQ=255)
                                                                ccTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGc                                                                                  <  2:7506490/151‑1 (MQ=255)
                                                                 cTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCa                                                                                 <  2:7374304/151‑1 (MQ=255)
                                                                 cTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCa                                                                                 >  2:4925832/1‑151 (MQ=255)
                                                                   ggCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATg                                                                               >  2:9475306/1‑151 (MQ=255)
                                                                    gcgcCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATgg                                                                              <  2:632666/151‑1 (MQ=255)
                                                                    gcgcCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATgg                                                                              >  1:5593191/1‑151 (MQ=255)
                                                                     cgcCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATggg                                                                             >  2:9555607/1‑151 (MQ=255)
                                                                      gcCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGa                                                                            <  1:8191172/151‑1 (MQ=255)
                                                                        cGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGTGACAGCGCTGCAACGCCAGGTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACt                                                                          >  2:6899634/1‑151 (MQ=255)
                                                                         gAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACCGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTg                                                                         >  2:5923617/1‑151 (MQ=255)
                                                                          aaCCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc                                                                        >  2:10964963/1‑151 (MQ=255)
                                                                          aaCCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc                                                                        <  2:3216805/151‑1 (MQ=255)
                                                                                                                  ggcctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGccc                                 <  2:1744636/144‑1 (MQ=255)
                                                                                                                  ggcctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGccc                                 >  2:3820880/8‑151 (MQ=255)
                                                                                                                    cctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTg                               >  2:6720296/6‑151 (MQ=255)
                                                                                                                    cctggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTg                               <  1:10094029/146‑1 (MQ=255)
                                                                                                                      tggccATCGAGGACGAGACAGCGCTGCACCGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGc                             <  2:656633/148‑1 (MQ=255)
                                                                                                                      tggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGc                             >  2:3869605/4‑151 (MQ=255)
                                                                                                                       ggccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGcc                            <  1:332531/149‑1 (MQ=255)
                                                                                                                        gccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGccc                           <  2:8907289/150‑1 (MQ=255)
                                                                                                                         ccATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCg                          <  2:1291866/151‑1 (MQ=255)
                                                                                                                            tCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCg                       >  2:8709152/1‑151 (MQ=255)
                                                                                                                            tCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCg                       >  1:9960187/1‑151 (MQ=255)
                                                                                                                              gAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCt                     >  2:3356516/1‑151 (MQ=255)
                                                                                                                              gAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCt                     >  1:678766/1‑151 (MQ=255)
                                                                                                                              gAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCt                     >  2:251568/1‑151 (MQ=255)
                                                                                                                                ggACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTc                   <  2:3929315/151‑1 (MQ=255)
                                                                                                                                ggACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTc                   >  1:10599638/1‑151 (MQ=255)
                                                                                                                                  aCGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTccc                 >  2:8540632/1‑151 (MQ=255)
                                                                                                                                   cGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCg                >  1:4344948/1‑151 (MQ=255)
                                                                                                                                     agaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGc              >  1:9138975/1‑151 (MQ=255)
                                                                                                                                     agaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGc              >  2:5154696/1‑151 (MQ=255)
                                                                                                                                     agaCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGc              <  2:10043700/151‑1 (MQ=255)
                                                                                                                                       aCAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCg            <  1:7609549/151‑1 (MQ=255)
                                                                                                                                        cAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGa           >  2:1842859/1‑151 (MQ=255)
                                                                                                                                        cAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGa           >  1:4512167/1‑151 (MQ=255)
                                                                                                                                         aGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGaa          <  1:8814341/151‑1 (MQ=255)
                                                                                                                                           cgcTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAAct        >  2:1611068/1‑151 (MQ=255)
                                                                                                                                           cgcTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAAct        >  2:2983081/1‑151 (MQ=255)
                                                                                                                                            gcTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAActg       >  1:7555966/1‑151 (MQ=255)
                                                                                                                                              tGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAActgct     <  1:233998/151‑1 (MQ=255)
                                                                                                                                              agCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAActgct     <  1:5394487/150‑1 (MQ=255)
                                                                                                                                               gCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAActgctg    >  1:8282302/1‑151 (MQ=255)
                                                                                                                                               gCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAActgctg    <  1:3928002/151‑1 (MQ=255)
                                                                                                                                                cAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGt   >  1:8533780/1‑151 (MQ=255)
                                                                                                                                                 aaCGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGtt  <  2:11675/151‑1 (MQ=255)
                                                                                                                                                 aaCGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGtt  >  1:5199671/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCGCCATCTGCGCCAGGTCCTCGAGTTGCCGCGCGCCGCCCTGGCCCGTCGCTTCCCGGCCGAACTGCTGTT  >  NC_008463/4947536‑4947830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑