Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,256,362 N→C ?82A (GNC→GCC)  dnaB → replicative DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,256,3620NC99.8% 1663.9 / NA 429?82A (GNC→GCC) dnaBreplicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base C (201/227);  minor base A (1/0);  total (202/227)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 433 total aligned reads displayed.

CGGCGATCAGTTGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCT  >  NC_008463/5256213‑5256511
                                                                                                                                                     |                                                                                                                                                     
cGGCGATCAGTTGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGcc                                                                                                                                                      <  2:6273524/151‑1 (MQ=255)
 ggCGATCAGTTGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCt                                                                                                                                                     <  2:5390589/151‑1 (MQ=255)
   cGATCAGTTGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTAc                                                                                                                                                   <  2:1554145/151‑1 (MQ=255)
   cGATCAGTTGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTAc                                                                                                                                                   >  2:10317510/1‑151 (MQ=255)
   cGATCAGTTGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTAc                                                                                                                                                   >  1:9961447/1‑151 (MQ=255)
      tCAGTTGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTc                                                                                                                                                >  1:7895853/1‑151 (MQ=255)
      tCAGTTGCAGAAGGAGGACTACTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTc                                                                                                                                                >  2:7396005/1‑151 (MQ=255)
         gTTGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGc                                                                                                                                             >  1:8758250/1‑151 (MQ=255)
           tGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCa                                                                                                                                           <  2:2101220/151‑1 (MQ=255)
            gCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAg                                                                                                                                          >  2:7672358/1‑151 (MQ=255)
              aGAAGGTGGACTTCTTCCGGAATGTGCATCGGCTGATCTTCACCGCCATCAGCGTGTTGGCGGCGTAGGATGCTGCGTTTGATGTCGTGGGTGTGGCGGTAGCGAGCGTTGACGTTGGTGAAGCTGGCGGGGTGGACGACCTCGGCGAgcg                                                                                                                                        >  2:1486288/1‑149 (MQ=255)
              aGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAgct                                                                                                                                        <  2:8906726/151‑3 (MQ=255)
               gAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTc                                                                                                                                       <  1:4770227/151‑1 (MQ=255)
               gAAGGAGGACTTCGTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTc                                                                                                                                       <  2:8977556/151‑1 (MQ=255)
                 aggaggACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGGGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgc                                                                                                                                     <  1:1270135/151‑3 (MQ=255)
                 aggaggACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgc                                                                                                                                     >  2:8059804/1‑149 (MQ=255)
                 aggaggACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgc                                                                                                                                     >  2:10676447/1‑149 (MQ=255)
                   gaggaCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTcgccg                                                                                                                                   >  1:1136261/1‑150 (MQ=255)
                       aCTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCcgacaa                                                                                                                               >  1:4369344/1‑151 (MQ=255)
                         ttcttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGacaaca                                                                                                                             >  2:7579490/1‑151 (MQ=255)
                           cttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACg                                                                                                                           >  2:3196181/1‑151 (MQ=255)
                           cttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACg                                                                                                                           >  2:3334567/1‑151 (MQ=255)
                           cttcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACg                                                                                                                           <  1:8356532/151‑1 (MQ=255)
                             tcCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGcc                                                                                                                         <  2:11175354/151‑1 (MQ=255)
                              ccGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGccc                                                                                                                        <  2:779660/151‑1 (MQ=255)
                                    tGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTg                                                                                                                  >  2:3812960/1‑151 (MQ=255)
                                    tGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTACGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTg                                                                                                                  <  2:9592406/151‑1 (MQ=255)
                                     gAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTgg                                                                                                                 <  2:2310091/151‑1 (MQ=255)
                                             gCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATc                                                                                                         <  2:10585243/151‑1 (MQ=255)
                                              cTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCg                                                                                                        >  2:5933842/1‑151 (MQ=255)
                                                gATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAg                                                                                                      <  2:8578434/151‑1 (MQ=255)
                                                gATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAg                                                                                                      >  1:3661458/1‑151 (MQ=255)
                                                 aTCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAgg                                                                                                     >  2:4088034/1‑151 (MQ=255)
                                                  tCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGc                                                                                                    >  2:6861061/1‑151 (MQ=255)
                                                   cTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCt                                                                                                   <  2:7247832/151‑1 (MQ=255)
                                                    ttCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCtt                                                                                                  <  1:1478012/151‑1 (MQ=255)
                                                    ttCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCtt                                                                                                  <  1:2804848/151‑1 (MQ=255)
                                                       aCCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTAcg                                                                                               >  2:3557876/1‑151 (MQ=255)
                                                          gCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACgcgc                                                                                            >  2:11037131/1‑151 (MQ=255)
                                                            cATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAg                                                                                          >  1:3765331/1‑151 (MQ=255)
                                                             aTCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGa                                                                                         <  2:9194366/151‑1 (MQ=255)
                                                              tCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGAt                                                                                        <  1:10972495/151‑1 (MQ=255)
                                                              tCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGAt                                                                                        >  1:7627263/1‑151 (MQ=255)
                                                              tCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGAt                                                                                        <  1:2235128/151‑1 (MQ=255)
                                                              tCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGAt                                                                                        >  2:694175/1‑151 (MQ=255)
                                                               cAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATc                                                                                       <  1:7370835/151‑1 (MQ=255)
                                                                aGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCg                                                                                      >  1:8893317/1‑151 (MQ=255)
                                                                aGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCg                                                                                      <  2:3097727/151‑1 (MQ=255)
                                                                  cGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGACCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGtt                                                                                    >  2:11230968/1‑151 (MQ=255)
                                                                     gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcgc                                                                                 >  1:1955136/1‑151 (MQ=255)
                                                                     gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcgc                                                                                 <  2:3693937/151‑1 (MQ=255)
                                                                     gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcgc                                                                                 <  1:7973350/151‑1 (MQ=255)
                                                                     gTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcgc                                                                                 >  2:4482368/1‑151 (MQ=255)
                                                                      ttGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTcgcg                                                                                >  1:1583711/1‑151 (MQ=255)
                                                                        ggCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGAt                                                                              >  1:10673307/1‑151 (MQ=255)
                                                                         gCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATc                                                                             >  1:10258604/1‑151 (MQ=255)
                                                                          ccGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCg                                                                            <  1:562545/151‑1 (MQ=255)
                                                                          ccGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCg                                                                            <  1:6443129/151‑1 (MQ=255)
                                                                             cgAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGc                                                                         >  2:2879652/1‑151 (MQ=255)
                                                                               aaGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGcac                                                                       <  1:4210911/151‑1 (MQ=255)
                                                                                 cgacgCTCCGCTTGCGGCCCTGACTGCGTCGCAAGCGCGCGACGACCTTCCAGACGCGGGCGGGCTGCCCTACCTCGGCCAGCTCGCCGACACCACGCCCACCGAGGCCAATATCGACGCTTACGCGCAGATCGTTCGCGATCGGGcaccc                                                                     <  2:10918377/147‑3 (MQ=255)
                                                                                   aTGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCt                                                                   >  2:1982963/1‑151 (MQ=255)
                                                                                   aTGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCt                                                                   <  2:10377237/151‑1 (MQ=255)
                                                                                   aTGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCt                                                                   >  1:9226144/1‑151 (MQ=255)
                                                                                     gCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGc                                                                 <  1:1874244/151‑1 (MQ=255)
                                                                                      cTCCGTATGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCg                                                                <  2:1761672/151‑1 (MQ=255)
                                                                                       tCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCgg                                                               >  1:10202197/1‑151 (MQ=255)
                                                                                         cGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGTCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCa                                                             >  2:5294324/1‑151 (MQ=255)
                                                                                           tttGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGc                                                           >  2:91585/1‑151 (MQ=255)
                                                                                             tGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTg                                                         >  1:5876951/1‑151 (MQ=255)
                                                                                                tGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATg                                                      >  1:1738080/1‑151 (MQ=255)
                                                                                                 gTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGt                                                     >  1:3120598/1‑151 (MQ=255)
                                                                                                  tCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtc                                                    >  2:1291688/1‑151 (MQ=255)
                                                                                                     tGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctct                                                 >  2:10820739/1‑151 (MQ=255)
                                                                                                      gACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGtctctc                                                <  1:9542554/151‑1 (MQ=255)
                                                                                                       aCTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCGACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCg                                               <  1:2428183/151‑1 (MQ=255)
                                                                                                         tgtgTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCggg                                             >  2:4862679/1‑151 (MQ=255)
                                                                                                         tgtgTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCggg                                             >  2:550667/1‑151 (MQ=255)
                                                                                                          gtgtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGc                                            <  1:8122421/151‑1 (MQ=255)
                                                                                                          gtgtCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGc                                            >  1:2647310/1‑151 (MQ=255)
                                                                                                              cGGCAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCGCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGcacca                                        <  2:2696384/151‑1 (MQ=255)
                                                                                                               ggAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGcaccac                                       >  2:4565649/1‑151 (MQ=255)
                                                                                                                 aaGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTg                                     <  1:5233632/151‑1 (MQ=255)
                                                                                                                 aaGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTg                                     <  1:3343651/151‑1 (MQ=255)
                                                                                                                 aaGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTg                                     >  1:6828613/1‑151 (MQ=255)
                                                                                                                     gATCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCAcc                                 <  1:2953694/151‑1 (MQ=255)
                                                                                                                      aTCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCa                                >  1:8263522/1‑151 (MQ=255)
                                                                                                                      aTCGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCa                                <  2:5073060/151‑1 (MQ=255)
                                                                                                                        cGAAGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAgg                              >  2:6155453/1‑151 (MQ=255)
                                                                                                                          aaGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAc                            <  2:8347570/151‑1 (MQ=255)
                                                                                                                           aGACCTTCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGAcc                           >  1:6302748/1‑151 (MQ=255)
                                                                                                                                ttCCAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTc                      <  1:6353280/151‑1 (MQ=255)
                                                                                                                                  ccAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGa                    >  2:6498889/1‑151 (MQ=255)
                                                                                                                                   cAGAAGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGaa                   <  1:7139675/151‑1 (MQ=255)
                                                                                                                                     gAAGCTGGCGGGCTGGCCTACCTCGGCCCGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAacc                 >  1:9806469/1‑151 (MQ=255)
                                                                                                                                      aaGCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAacca                <  1:243736/151‑1 (MQ=255)
                                                                                                                                        gCTGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAaccacc              <  1:10530902/151‑1 (MQ=255)
                                                                                                                                          tGGCGGGCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAg            <  2:8698577/151‑1 (MQ=255)
                                                                                                                                               ggCTGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCaaa       <  2:8494903/151‑1 (MQ=255)
                                                                                                                                                  tGGCCTACCTCGGCCAGCTCGCCGACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATcc    >  2:8174255/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCGATCAGTTGCAGAAGGAGGACTTCTTCCGGCATGAGCATCGGCTGATCTTCACCGCCATCAGCGAGTTGGCCGCGAAGGATGCTCCGTTTGATGTCGTGACTGTGTCGGAAGCGATCGAAGACCTTCCAGAAGCTGGCGGGCTGGNCTACCTCGGCCAGTTCCCCAACAACACGCCCTCCGTGGCCAATATCGAGGCTTACGCGCAGATCGTTCGCGATCGGGCACACCTGCGGCAGCTGATGTCTCTCGGGCACCACTGCACCAGGACCGCCTCGAACCACCAGGCAAATCCCT  >  NC_008463/5256213‑5256511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑