Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,791,938 A→G intergenic (+32/‑103) nadE → / → choE ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,791,9380AG100.0% 2252.4 / NA 563intergenic (+32/‑103)nadE/choEammonia‑dependent NAD(+) synthetase/cholinesterase ChoE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (292/271);  total (292/271)

Only 100 of 563 total aligned reads displayed.

TATGCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCG  >  NC_008463/5791790‑5792086
                                                                                                                                                    |                                                                                                                                                    
   gCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACggg                                                                                                                                                 >  2:2095833/1‑151 (MQ=255)
   gCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACggg                                                                                                                                                 <  2:2909186/151‑1 (MQ=255)
    ccGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGc                                                                                                                                                <  2:2719050/151‑1 (MQ=255)
        gaTCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACCAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCttt                                                                                                                                            <  2:523564/151‑1 (MQ=255)
           cGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTc                                                                                                                                         >  1:921626/1‑151 (MQ=255)
            gACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTcg                                                                                                                                        <  1:1194447/151‑1 (MQ=255)
             aCGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTcgc                                                                                                                                       <  1:5661465/151‑1 (MQ=255)
               gCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcgg                                                                                                                                     <  2:9092880/151‑1 (MQ=255)
                   tCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCTACACCTACCACAAGTCCCAGCACAAGCGCGAACTGCCGAAGGCGCCCGGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcgggcgg                                                                                                                                 >  1:5317601/1‑151 (MQ=255)
                   tCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCgcgggcgg                                                                                                                                 <  1:11037662/151‑1 (MQ=255)
                          cGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAAtgtg                                                                                                                          <  2:6687081/151‑1 (MQ=255)
                           ggCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGc                                                                                                                         >  2:885811/1‑151 (MQ=255)
                             ccAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCt                                                                                                                       <  1:1505276/151‑1 (MQ=255)
                              cAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTg                                                                                                                      >  1:9411827/1‑151 (MQ=255)
                              cAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTg                                                                                                                      <  1:8241738/151‑1 (MQ=255)
                              cAGCCGCGGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTg                                                                                                                      <  2:5959776/151‑1 (MQ=255)
                                gCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTc                                                                                                                    >  1:1005250/1‑151 (MQ=255)
                                 ccGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTcg                                                                                                                   >  1:5611703/1‑150 (MQ=255)
                                  cGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTcgc                                                                                                                  >  2:4433742/1‑149 (MQ=255)
                                  cGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTcgc                                                                                                                  >  2:5153939/1‑149 (MQ=255)
                                   gCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTcgca                                                                                                                 >  1:6972228/1‑148 (MQ=255)
                                    cTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTcgcaa                                                                                                                >  2:10399621/1‑147 (MQ=255)
                                     tGCGCGACGAAGCCGCGCGGGTGATCGTCGACACCTACCACACGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAAc                                                                                                              <  2:7015216/151‑1 (MQ=255)
                                      gcgcGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACCAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAAcc                                                                                                             <  2:10600636/151‑1 (MQ=255)
                                      gcgcGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAAcc                                                                                                             <  1:2659554/151‑1 (MQ=255)
                                      gcgcGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAAcc                                                                                                             <  1:10390626/151‑1 (MQ=255)
                                       cgcgAGGACGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCa                                                                                                            >  2:3147076/1‑151 (MQ=255)
                                       cgcgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCa                                                                                                            <  2:9394062/151‑1 (MQ=255)
                                         cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAgg                                                                                                          <  1:9067696/151‑1 (MQ=255)
                                         cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAgg                                                                                                          <  1:384905/151‑1 (MQ=255)
                                         cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAgg                                                                                                          <  1:2005375/151‑1 (MQ=255)
                                         cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAgg                                                                                                          >  1:7164845/1‑151 (MQ=255)
                                         cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCGTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAgg                                                                                                          >  1:6470107/1‑151 (MQ=255)
                                             gAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCtt                                                                                                      <  2:8092832/151‑1 (MQ=255)
                                               aGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCa                                                                                                    >  2:136037/1‑151 (MQ=255)
                                                 ccGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAg                                                                                                  >  2:9802252/1‑151 (MQ=255)
                                                   gcgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTc                                                                                                <  1:9529856/151‑1 (MQ=255)
                                                   gcgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCGTTGTCGCGGGCGGGAATGTGCCTGTC‑GCACCCAGGTCTTCAAGTc                                                                                                >  2:5605871/1‑151 (MQ=255)
                                                    cgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTcc                                                                                               >  1:5452885/1‑151 (MQ=255)
                                                     gcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcg                                                                                              <  1:286895/151‑1 (MQ=255)
                                                       gggTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcg                                                                                            >  1:6635119/1‑151 (MQ=255)
                                                       gggTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcg                                                                                            >  1:3379458/1‑151 (MQ=255)
                                                        ggTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCAGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGAGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcgc                                                                                           >  1:1687734/1‑151 (MQ=255)
                                                           gATCGTCGACACCTACCCCAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggc                                                                                        <  2:7155575/151‑1 (MQ=255)
                                                           gATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggc                                                                                        <  1:7500386/151‑1 (MQ=255)
                                                            aTCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcg                                                                                       >  2:5810058/1‑151 (MQ=255)
                                                              cgtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggc                                                                                     <  2:3706639/151‑1 (MQ=255)
                                                              cgtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggc                                                                                     >  1:3139073/1‑151 (MQ=255)
                                                               gtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggcg                                                                                    <  1:7390346/151‑1 (MQ=255)
                                                                 cgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGgcgcg                                                                                  <  2:4826236/151‑1 (MQ=255)
                                                                   acacCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCt                                                                                >  1:8354656/1‑151 (MQ=255)
                                                                    cacCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTa                                                                               >  1:2163075/1‑151 (MQ=255)
                                                                       cTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTagaa                                                                            >  2:7283522/1‑151 (MQ=255)
                                                                         aCCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAgg                                                                          <  2:3822617/151‑1 (MQ=255)
                                                                          ccACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGa                                                                         >  2:3269284/1‑151 (MQ=255)
                                                                             caAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAAGCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGACGc                                                                      >  2:7472874/1‑151 (MQ=255)
                                                                              aaGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCt                                                                     <  1:4002716/151‑1 (MQ=255)
                                                                                 aCCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGaa                                                                  >  1:3737999/1‑151 (MQ=255)
                                                                                  cccAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCGAGAAGGAAGCTGAAt                                                                 >  1:4836618/1‑151 (MQ=255)
                                                                                   ccAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATc                                                                >  2:4561789/1‑151 (MQ=255)
                                                                                   ccAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATc                                                                <  1:5908026/151‑1 (MQ=255)
                                                                                     aGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATccc                                                              >  2:6041594/1‑151 (MQ=255)
                                                                                         caAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCg                                                          >  1:5793778/1‑151 (MQ=255)
                                                                                            gcgaGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAg                                                       <  2:4329535/151‑1 (MQ=255)
                                                                                              gcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgc                                                     >  1:11150508/1‑151 (MQ=255)
                                                                                              gcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgc                                                     >  1:8677763/1‑151 (MQ=255)
                                                                                              gcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgc                                                     <  1:429644/151‑1 (MQ=255)
                                                                                               cgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCTGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgcc                                                    >  1:243346/1‑151 (MQ=255)
                                                                                                gAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgcca                                                   >  2:8736857/1‑151 (MQ=255)
                                                                                                 aaCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccag                                                  >  1:9591853/1‑151 (MQ=255)
                                                                                                 aaCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCATTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccag                                                  <  1:1050964/151‑1 (MQ=255)
                                                                                                    tGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagcca                                               <  2:10090761/151‑1 (MQ=255)
                                                                                                    tGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCGAGAAGGAAGCTGAATCCCTGCGAAGgccagcca                                               >  2:4079135/1‑151 (MQ=255)
                                                                                                      ccGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATg                                             <  1:4202610/151‑1 (MQ=255)
                                                                                                        gAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcg                                           >  1:4676697/1‑151 (MQ=255)
                                                                                                         aaGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcgc                                          >  1:9903767/1‑151 (MQ=255)
                                                                                                         aaGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcgc                                          <  1:9822053/151‑1 (MQ=255)
                                                                                                         aaGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcgc                                          >  1:10836093/1‑151 (MQ=255)
                                                                                                              gcCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCg                                     <  2:1645742/151‑1 (MQ=255)
                                                                                                                ccTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtc                                   <  2:1739203/151‑1 (MQ=255)
                                                                                                                  tGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtctc                                 <  1:6241009/151‑1 (MQ=255)
                                                                                                                    aGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTccc                               >  1:1365842/1‑151 (MQ=255)
                                                                                                                     gCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCATTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTcccc                              <  2:6116131/151‑1 (MQ=255)
                                                                                                                      ccTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCg                             >  1:8874819/1‑151 (MQ=255)
                                                                                                                      ccTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCATTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCg                             <  1:4951483/151‑1 (MQ=255)
                                                                                                                       cTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGc                            >  2:5095254/1‑151 (MQ=255)
                                                                                                                        tCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGcc                           >  2:7978510/1‑151 (MQ=255)
                                                                                                                              cccGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctc                     <  1:10224040/151‑1 (MQ=255)
                                                                                                                               ccGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCAGGCGCACCCGTCTCCCCGCCCTGctcc                    >  1:783904/1‑151 (MQ=255)
                                                                                                                                 gTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcctc                  >  2:8444637/1‑151 (MQ=255)
                                                                                                                                    cGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGgt               <  1:3017433/151‑1 (MQ=255)
                                                                                                                                    cGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGgt               <  2:8255528/151‑1 (MQ=255)
                                                                                                                                     ggCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGgtg              <  2:243798/151‑1 (MQ=255)
                                                                                                                                       cAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTc            >  2:7688259/1‑151 (MQ=255)
                                                                                                                                        aGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTcc           >  1:10282383/1‑151 (MQ=255)
                                                                                                                                          gCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctg         >  1:2036420/1‑151 (MQ=255)
                                                                                                                                           cccGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgc        >  2:1816729/1‑151 (MQ=255)
                                                                                                                                             cgtcgtCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgctg      >  2:10835669/1‑151 (MQ=255)
                                                                                                                                             cgtcgtCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTAAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgctg      >  1:8188705/1‑151 (MQ=255)
                                                                                                                                                 gtcGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccg  >  1:9485549/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATGCCGAGATCGACGCCTTCCTGCACGGCCAGCCGCTGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCG  >  NC_008463/5791790‑5792086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑