Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,809,318 G→C intergenic (‑204/+15) rpsF ← / ← rlmB 30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,809,3180GC100.0% 1407.8 / NA 371intergenic (‑204/+15)rpsF/rlmB30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (219/152);  total (219/152)

Only 100 of 371 total aligned reads displayed.

CTTGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGC  >  NC_008463/5809174‑5809462
                                                                                                                                                |                                                                                                                                                
tttcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCg                                                                                                                                           <  2:9647771‑M2/26‑1 (MQ=255)
tatcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggatgattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCg                                                                                                                                           <  2:1779275‑M2/26‑1 (MQ=255)
 ttcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatagttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGc                                                                                                                                          >  1:5463353‑M2/125‑151 (MQ=255)
  tcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCa                                                                                                                                         <  1:10948885‑M2/28‑1 (MQ=255)
   cttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAc                                                                                                                                        <  2:2976002‑M2/29‑1 (MQ=255)
   cttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAc                                                                                                                                        >  1:11110681‑M2/123‑151 (MQ=255)
       gcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgccattcgagagacgggcaaccggcgaagcaaggaagattggcgcatattggtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACgcgc                                                                                                                                    >  2:305831‑M2/119‑151 (MQ=255)
       gcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACgcgc                                                                                                                                    >  2:9548855‑M2/119‑151 (MQ=255)
         ttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTc                                                                                                                                  >  1:7121506‑M2/117‑151 (MQ=255)
          tgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCCGGG‑CCTCGCCGCGGCGGCCCCGCACGCGCTCa                                                                                                                                 >  1:8693050‑M2/116‑151 (MQ=255)
           gcctggtgtagaggcgaacgaacgcctgcccgcgcggcatggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCAc                                                                                                                                <  1:2941701‑M2/37‑1 (MQ=255)
           gcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggctaatatttgtaaagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCAc                                                                                                                                <  2:1373520‑M2/37‑1 (MQ=255)
           gcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCAc                                                                                                                                >  2:7258915‑M2/115‑151 (MQ=255)
             ctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTc                                                                                                                              >  1:7684617‑M2/113‑151 (MQ=255)
             ctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTc                                                                                                                              >  2:8155939‑M2/113‑151 (MQ=255)
             ctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTc                                                                                                                              >  1:11016598‑M2/113‑151 (MQ=255)
                gtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAg                                                                                                                           >  2:10263620‑M2/110‑151 (MQ=255)
                 tggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGc                                                                                                                          >  1:3528020‑M2/109‑151 (MQ=255)
                 tggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGc                                                                                                                          <  1:5897082‑M2/43‑1 (MQ=255)
                 tggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGc                                                                                                                          <  1:3921793‑M2/43‑1 (MQ=255)
                  ggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattatagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCg                                                                                                                         <  2:1547701‑M2/44‑1 (MQ=255)
                    agaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgactatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCggg                                                                                                                       >  1:5059868‑M2/106‑151 (MQ=255)
                     gaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCgggg                                                                                                                      >  2:7168481‑M2/105‑151 (MQ=255)
                     gaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCgggg                                                                                                                      >  2:9747301‑M2/105‑151 (MQ=255)
                      aggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                     >  1:9462635‑M2/104‑151 (MQ=255)
                        gcgaacgaacgcctgcccgcgcggcaaggggcgacagtctagagaagggcaaccggcgaagcaaggaagattggcgaatattggtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgc                                                                                                                   <  2:5034296‑M2/50‑1 (MQ=255)
                          gaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgcgc                                                                                                                 >  1:10173520‑M2/100‑151 (MQ=255)
                          gaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTgcgc                                                                                                                 >  2:7483830‑M2/100‑151 (MQ=255)
                            acgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTg                                                                                                               <  1:2187527‑M2/54‑1 (MQ=255)
                               aacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCg                                                                                                            <  1:10774095‑M2/57‑1 (MQ=255)
                                 cgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCgga                                                                                                          <  2:5541520‑M2/59‑1 (MQ=255)
                                  gcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggag                                                                                                         >  2:3256666‑M2/92‑151 (MQ=255)
                                     tgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGAt                                                                                                      <  1:11174236‑M2/63‑1 (MQ=255)
                                             cggcaaggggcgagattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACa                                                                                              >  1:3172395‑M2/81‑151 (MQ=255)
                                               gcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAgg                                                                                            >  2:1705558‑M2/79‑151 (MQ=255)
                                                caaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGc                                                                                           >  2:3095911‑M2/78‑151 (MQ=255)
                                                 gggCGACATTCTAGAGCAGGGCACCCGGCGCAGCAAGGAAGATTGGCGAATATTGGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCCCTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAc                                                                                       <  1:9035955/151‑1 (MQ=255)
                                                  aggggcgacattctagcgaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAg                                                                                         <  1:9784215‑M2/76‑1 (MQ=255)
                                                  aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgactatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAg                                                                                         >  2:6908738‑M2/76‑151 (MQ=255)
                                                     ggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACg                                                                                      >  1:325442‑M2/73‑151 (MQ=255)
                                                     ggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACg                                                                                      >  1:8995469‑M2/73‑151 (MQ=255)
                                                          cattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGt                                                                                 >  2:8941053‑M2/68‑151 (MQ=255)
                                                           attctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTg                                                                                >  2:1434953‑M2/67‑151 (MQ=255)
                                                            ttctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTgg                                                                               >  1:6686860‑M2/66‑151 (MQ=255)
                                                             tctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGc                                                                              >  1:10186300‑M2/65‑151 (MQ=255)
                                                              ctagagaagcgcaaccggcgaagcaaggaagattggcgaatatttggacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                             <  2:5892420‑M2/88‑1 (MQ=255)
                                                               tagagaagggcaaccggcgaagcaaggaagattggcgaatagttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGa                                                                            >  1:2476125‑M2/63‑151 (MQ=255)
                                                                gacgggCAACCGGCGAAGCAAGGAAGATAGGCGAATATTTGTCCAGCGGATGGAATG‑‑‑GCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCCCACTCCAGCGGGGTGCGCTGGCGGAGGCTCTCGACCAGGCAGACGCCGGTGGCGACCga                                                                        <  2:4293814/148‑1 (MQ=255)
                                                                agagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                           >  2:10105616‑M2/62‑151 (MQ=255)
                                                                 gagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAcc                                                                          <  1:8658709‑M2/91‑1 (MQ=255)
                                                                     gCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑CCGCGCCGCGGCGGCCCCGGACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGGTCTCGTACAGGGCGACGCCGGGGGCGTGCGAGTCGt                                                                   >  1:5846393/1‑151 (MQ=255)
                                                                     agggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgaga                                                                      >  1:4112607‑M2/57‑151 (MQ=255)
                                                                     agggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCGCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgaga                                                                      >  1:6236410‑M2/57‑151 (MQ=255)
                                                                      gggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGAc                                                                     <  1:4869910‑M2/96‑1 (MQ=255)
                                                                       ggcaaccggcgaagcacggaagattggcgactagttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACg                                                                    <  2:8464903‑M2/97‑1 (MQ=255)
                                                                       aaCCGGCGAAGCAAGGAAGATTGCCGAATCTTTGAACAGCGGATGGAAT‑GGCGC‑CGGG‑‑CCGCCACGCGGCCGCCCCGCACGCGCTCAAGCCAGCGGGGTGCCCTGGCGGAGGAACACACACAGGCACACCCCGGTGGCCACCGAGACGTTc                                                                 >  1:1452813/1‑151 (MQ=255)
                                                                          aaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                 >  1:289594‑M2/52‑151 (MQ=255)
                                                                           accggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGGCGTTCa                                                                <  1:9233998‑M2/101‑1 (MQ=255)
                                                                           accggcgaagcaaggaagattggcgaatattggtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCa                                                                <  1:6080880‑M2/101‑1 (MQ=255)
                                                                            ccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAg                                                               >  2:9176045‑M2/50‑151 (MQ=255)
                                                                             cggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGTTCTCGAACGGGCCGACGCCGGTGGCGACCGAGACGTTCAgg                                                              >  1:9703732‑M2/49‑151 (MQ=255)
                                                                             cggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAgg                                                              <  2:8802586‑M2/103‑1 (MQ=255)
                                                                              ggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                             >  2:10749482‑M2/48‑151 (MQ=255)
                                                                              ggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                             >  2:5784394‑M2/48‑151 (MQ=255)
                                                                               gcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                            <  2:8133360‑M2/105‑1 (MQ=255)
                                                                                    gcaaggaagattggcgaatattggtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGa                                                       <  2:7068752‑M2/110‑1 (MQ=255)
                                                                                      gAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑CCCCGCCGCGGCGGCCCCGCACCCGCACAAACCCACGGGGGGCGCCAGCGGTGGATCTCGAACACGCAGACGCCGGTCGCGACCGAGACCTTCAGCCGGCAGACGCTg                                                  >  1:1904056/1‑151 (MQ=255)
                                                                                        ggaagattggcgactatgggtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGCCCGAGACGTTCAGGCTGCTGACgcg                                                   <  1:8828371‑M2/114‑2 (MQ=255)
                                                                                         gaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGGGCTCACTCCCGCGGGGTGCGCGGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGGTCAGGCTGCTGACGCTg                                                  >  2:390374‑M2/37‑151 (MQ=255)
                                                                                           agattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGcc                                                <  2:10990312‑M2/117‑1 (MQ=255)
                                                                                             cttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCACGAACAGGCAGACGCCGGTGGCGACCGAGACGGTCAGGCTGCGGACGCTGCCgg                                              >  1:5365315‑M2/33‑151 (MQ=255)
                                                                                                 gcgaatatttgtacagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAt                                          <  2:5824076‑M2/123‑1 (MQ=255)
                                                                                                   gaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCg                                        >  2:4098570‑M2/27‑151 (MQ=255)
                                                                                                    aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                       >  2:2382664‑M2/26‑151 (MQ=255)
                                                                                                     atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                      <  2:3551729‑M2/127‑1 (MQ=255)
                                                                                                     atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                      >  1:10500222‑M2/25‑151 (MQ=255)
                                                                                                     atatgtgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGACCAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                      <  1:6914409‑M2/127‑1 (MQ=255)
                                                                                                         ttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGTCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGTCGCTGCCGGCCAGCGGCCAcc                                  >  1:4845046‑M2/21‑151 (MQ=255)
                                                                                                          tgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGTCGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCt                                 >  1:4596742‑M2/20‑151 (MQ=255)
                                                                                                           gtacagaggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCTCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGGTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTg                                >  2:9155393‑M2/19‑151 (MQ=255)
                                                                                                              cagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCACACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCg                             >  1:7438235‑M2/16‑151 (MQ=255)
                                                                                                               agcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGa                            <  2:2260678‑M2/137‑1 (MQ=255)
                                                                                                                 cggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGTCCGAGACGATCAGGCTGCTGACGCTGCCGGCCATCGGCAACCAGGCGAgg                          >  1:2679355‑M2/13‑151 (MQ=255)
                                                                                                                    atggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAg                       <  1:8249251‑M2/142‑1 (MQ=255)
                                                                                                                     tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGAGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCCTCGGCAACCAGGCTAGGAagg                      >  2:6403600‑M2/9‑150 (MQ=255)
                                                                                                                      ggcatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGGGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTc                     <  2:5676070‑M2/144‑1 (MQ=255)
                                                                                                                      ggaatggCGCGGGGCCGCGCCC‑CGGCGGCCCCCCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTTACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTc                     >  1:7857583‑M2/8‑151 (MQ=255)
                                                                                                                      ggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGATCTCGAACCTGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTc                     >  2:10493951‑M2/8‑151 (MQ=255)
                                                                                                                      ggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTc                     <  1:10297011‑M2/144‑1 (MQ=255)
                                                                                                                       gaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCg                    >  2:9862725‑M2/7‑151 (MQ=255)
                                                                                                                        aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCGGCCGGCCATCGGCCACCTGGCGAGGAAGTCGc                   >  2:4490741‑M2/6‑151 (MQ=255)
                                                                                                                          tggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAg                 >  2:6435832‑M2/4‑151 (MQ=255)
                                                                                                                             cgcgGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGc              <  2:2696887/151‑1 (MQ=255)
                                                                                                                                   ggacgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGTGGTTCTCGATCAGGCTGACGCCGGTGGCGACCGTGACGTTCTGGCTGCTGACGCTGCCGGCCATCGTCAACCTGGCGTGGTTGTCGCTGTGCTcgcg         <  2:2786680/144‑1 (MQ=255)
                                                                                                                                     ccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCggg       <  1:835883/146‑1 (MQ=255)
                                                                                                                                      cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt      >  1:3565742/5‑151 (MQ=255)
                                                                                                                                        cgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCa    >  2:4233774/3‑151 (MQ=255)
                                                                                                                                        cgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCa    <  2:2515949/149‑1 (MQ=255)
                                                                                                                                         gccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg   >  1:3832077/2‑151 (MQ=255)
                                                                                                                                         gccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg   >  2:5245588/2‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTTGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGC  >  NC_008463/5809174‑5809462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑