Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,809,365 T→C K239E (AAG→GAG)  rlmB ← 23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,809,3650TC99.1% 1597.2 / ‑5.1 425K239E (AAG→GAG) rlmB23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (237/184);  minor base A (4/0);  total (241/184)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 431 total aligned reads displayed.

AGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGT  >  NC_008463/5809221‑5809512
                                                                                                                                                |                                                                                                                                                   
gcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGCGGCACTCGAACAgg                                                                                                                                              >  2:7306048‑M2/79‑151 (MQ=255)
   aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgactatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAg                                                                                                                                           >  2:6908738‑M2/76‑151 (MQ=255)
      ggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACg                                                                                                                                        >  1:8995469‑M2/73‑151 (MQ=255)
      ggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACg                                                                                                                                        >  1:325442‑M2/73‑151 (MQ=255)
       gcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGc                                                                                                                                       >  2:9465513‑M2/72‑151 (MQ=255)
        cgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGcc                                                                                                                                      <  1:10177296‑M2/81‑1 (MQ=255)
           cattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGt                                                                                                                                   >  2:8941053‑M2/68‑151 (MQ=255)
            attctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTg                                                                                                                                  >  2:1434953‑M2/67‑151 (MQ=255)
               ctagagaagggcaaccggcgaagcaaggaagattggcgaagatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                                                                               >  2:7995263‑M2/64‑151 (MQ=255)
               ctagagaagcgcaaccggcgaagcaaggaagattggcgaatatttggacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                                                                               <  2:5892420‑M2/88‑1 (MQ=255)
                 agagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                                                                             >  2:10105616‑M2/62‑151 (MQ=255)
                    gaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCga                                                                                                                          <  2:5177928‑M2/93‑1 (MQ=255)
                     acgggcaaccggcgaagcaaggaagattggcgaatattagtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgag                                                                                                                         <  1:7456846‑M2/94‑1 (MQ=255)
                     aagggcaaccggcgaagctaggaagaatggcgaatacttgtaccgcgtatggaagggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgag                                                                                                                         <  1:1839786‑M2/94‑1 (MQ=255)
                         gcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGt                                                                                                                     >  1:1615026‑M2/54‑151 (MQ=255)
                           aaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTc                                                                                                                   >  2:2749400‑M2/52‑151 (MQ=255)
                               ggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgc                                                                                                               >  2:5784394‑M2/48‑151 (MQ=255)
                                gcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                                                                              >  1:4107780‑M2/47‑151 (MQ=255)
                                 cgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctg                                                                                                             <  1:2601987‑M2/106‑1 (MQ=255)
                                      caaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGAc                                                                                                        <  2:9159028‑M2/111‑1 (MQ=255)
                                       aaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGAGACCGAGACGTTCAGGCTGATGACg                                                                                                       >  2:6125651‑M2/40‑151 (MQ=255)
                                          gaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTg                                                                                                    >  1:7062451‑M2/37‑151 (MQ=255)
                                           aagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCGCCGCACGCGCTCACTACAGCGGGGTGCGCTGGCGGGGGATCTAGCACAGGCCGACGCCGGTGGCGACCGAGACGGTCAGGCTGCGGACGCTGc                                                                                                   >  1:6857993‑M2/36‑151 (MQ=255)
                                             gattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCg                                                                                                 <  1:9705119‑M2/118‑1 (MQ=255)
                                               ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGCCCGAGACGTTCAGGCTGCTGACGCTGCCGGc                                                                                               >  2:3715057‑M2/32‑151 (MQ=255)
                                               ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGc                                                                                               <  2:7075489‑M2/120‑1 (MQ=255)
                                                 gAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑CCGCGCCGCGGCGGCCCCCCACCCGCGCACTCCAGCGGGGTGCGCTGGCGGAGGATCTAGAAAATGCATACGCCGGTGGCGACCCACTCGTTCAGGCTGCTGAAGCTGCCGGCCAACg                                                                                          >  1:497184/1‑151 (MQ=255)
                                                  gcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCACCCGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGAAGCTGCCGGCCAt                                                                                            >  1:5308145‑M2/29‑151 (MQ=255)
                                                    gaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCg                                                                                          >  2:4098570‑M2/27‑151 (MQ=255)
                                                    gaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCGCACACCCGCGGGGTGCGCTGGCGGAGGATCTCGAAAAGGCAGACGCCGGTGGCGTCCGAGACGGTCAGGCTGCTGACGCTGCCGGCCATCg                                                                                          >  1:3733880‑M2/27‑151 (MQ=255)
                                                      atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                                                                        <  2:9877315‑M2/127‑1 (MQ=255)
                                                      atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                                                                        <  2:3551729‑M2/127‑1 (MQ=255)
                                                      atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGATACGTTCAGGCTGCTGACGCTGCCGGCCAGCGGc                                                                                        >  2:3917484‑M2/25‑151 (MQ=255)
                                                         tttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGCCGTTCAGGCTGCTGACGCTGCCGCCCATCGGCAAc                                                                                     >  2:4449645‑M2/22‑151 (MQ=255)
                                                         tttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAAc                                                                                     >  1:11078596‑M2/22‑151 (MQ=255)
                                                          ttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGTCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGTCGCTGCCGGCCAGCGGCCAcc                                                                                    >  1:4845046‑M2/21‑151 (MQ=255)
                                                           tgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCCCACCCGCGCACACCAGCGGGGTGCGCTGGCGGTGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCGGACGCTGCCGGCCATCGGCAACCt                                                                                   >  1:10423904‑M2/20‑151 (MQ=255)
                                                             tacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTgg                                                                                 <  1:6590737‑M2/134‑1 (MQ=255)
                                                              acagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATAGGCAACCTGGc                                                                                >  2:8287975‑M2/17‑151 (MQ=255)
                                                               cagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCCTCGGCCACCTGGCg                                                                               >  1:9858611‑M2/16‑151 (MQ=255)
                                                               cagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCg                                                                               >  1:3108844‑M2/16‑151 (MQ=255)
                                                                 gcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCCCGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGGGGCGACCGAGACGTTCAGGCTGCGGACGCTGCCGGCCCTCGGCAACCTGGCGAg                                                                             >  1:9259667‑M2/14‑151 (MQ=255)
                                                                 gcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAg                                                                             <  1:8640131‑M2/138‑1 (MQ=255)
                                                                  cggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAgg                                                                            >  2:9330428‑M2/13‑151 (MQ=255)
                                                                    gatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGGTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGaa                                                                          <  1:7234913‑M2/141‑1 (MQ=255)
                                                                      tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGAGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCCTCGGCAACCAGGCTAGGAagg                                                                        >  2:6403600‑M2/9‑150 (MQ=255)
                                                                        gaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCg                                                                      >  2:4246537‑M2/7‑151 (MQ=255)
                                                                         aatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGTGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTGCCGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGc                                                                     >  2:10993342‑M2/6‑151 (MQ=255)
                                                                              cgcgGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGc                                                                >  2:8492383/1‑151 (MQ=255)
                                                                               gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt                                                               <  1:11149749/151‑1 (MQ=255)
                                                                               gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt                                                               >  1:8712782/1‑151 (MQ=255)
                                                                               gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt                                                               <  1:4418024/151‑1 (MQ=255)
                                                                               gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCt                                                               <  1:2923393/151‑1 (MQ=255)
                                                                                    ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg                                                           <  2:2802480/144‑1 (MQ=255)
                                                                                    ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg                                                           >  1:909050/8‑151 (MQ=255)
                                                                                    ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg                                                           >  2:10811938/8‑151 (MQ=255)
                                                                                     gccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCgg                                                          >  2:1806743/7‑151 (MQ=255)
                                                                                      ccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCggg                                                         <  1:835797/146‑1 (MQ=255)
                                                                                       cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt                                                        >  1:3565742/5‑151 (MQ=255)
                                                                                        gcgccGCGGCGGCCCCGCACGCGCTCACTACAGCGGGGTGCGCTGGCGGAGGATCTCGCACAGGCAGAAGCCGGAGGCGAACGAGACGTACAGGCTGCTGACGCTGCCGGCCATCGTCAACGTGGCGAGGACGACGAGGTGCTCGCGGGTc                                                       >  2:1604933/4‑151 (MQ=255)
                                                                                         cgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCa                                                      <  2:5443327/149‑1 (MQ=255)
                                                                                          gccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAg                                                     >  2:2395447/2‑151 (MQ=255)
                                                                                             gcggcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCg                                                  <  1:818672/146‑1 (MQ=255)
                                                                                                gcggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGgcg                                               >  2:10627708/3‑151 (MQ=255)
                                                                                                 cggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGgcgc                                              >  2:8801691/2‑151 (MQ=255)
                                                                                                  ggCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCa                                             >  2:565762/1‑151 (MQ=255)
                                                                                                   gCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCAt                                            >  1:3871897/1‑151 (MQ=255)
                                                                                                    ccccGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATg                                           >  2:884297/1‑151 (MQ=255)
                                                                                                     gccGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCCGCCGGCGCATGc                                          >  1:318091/2‑151 (MQ=255)
                                                                                                     cccGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGACGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGc                                          >  2:9717675/1‑151 (MQ=255)
                                                                                                        gCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGACGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCt                                       >  1:7815089/1‑151 (MQ=255)
                                                                                                        gCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCt                                       >  1:7995921/1‑151 (MQ=255)
                                                                                                        ccACGCGCTCACTCCAGCGGGCTGCGCTGGCGGCGCATCGCGAACAGGCCGCCGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCCGCCGGCCAACGGCAACCGGGCGAGGAAGTCGCAGTGCCCGCGGGTCCGCCGGCGCATGCCCt                                       <  2:1955526/150‑1 (MQ=255)
                                                                                                         cACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCACCCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCtt                                      >  1:10236882/1‑151 (MQ=255)
                                                                                                         cACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCtt                                      <  2:6477074/151‑1 (MQ=255)
                                                                                                           cgcgCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGc                                    >  1:10543692/1‑151 (MQ=255)
                                                                                                           cgcgCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGc                                    <  2:1380919/151‑1 (MQ=255)
                                                                                                              gcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACCGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCtt                                 <  2:2578440/151‑1 (MQ=255)
                                                                                                              gcTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCtt                                 >  2:4374433/1‑151 (MQ=255)
                                                                                                                tCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTcc                               <  1:582051/151‑1 (MQ=255)
                                                                                                                  acgccAGCGGGGTGCGCTGGCGGAGGATCTCGACCAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcgc                             <  2:2284844/148‑1 (MQ=255)
                                                                                                                   cTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCcgcg                            <  1:5995157/151‑1 (MQ=255)
                                                                                                                     ccAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGcc                          <  2:10500222/151‑1 (MQ=255)
                                                                                                                      cAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGccc                         >  1:10905974/1‑151 (MQ=255)
                                                                                                                      cAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGccc                         >  1:6227625/1‑151 (MQ=255)
                                                                                                                       aGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCa                        >  1:7380069/1‑151 (MQ=255)
                                                                                                                       aGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCa                        <  1:547201/151‑1 (MQ=255)
                                                                                                                         cGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATc                      >  1:2428417/1‑151 (MQ=255)
                                                                                                                           gggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCAc                    <  2:7216912/151‑1 (MQ=255)
                                                                                                                            ggTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCAcc                   <  1:2456318/151‑1 (MQ=255)
                                                                                                                             gTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCa                  <  1:9669366/151‑1 (MQ=255)
                                                                                                                              tGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCATGCCTTCCGCGCCCATCACCAg                 >  1:4550133/1‑151 (MQ=255)
                                                                                                                               gcgcTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGc                >  2:9615694/1‑151 (MQ=255)
                                                                                                                                    ggCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGt           <  2:6811772/151‑1 (MQ=255)
                                                                                                                                    ggCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGt           <  2:3156417/151‑1 (MQ=255)
                                                                                                                                      cGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCg         <  2:10361727/151‑1 (MQ=255)
                                                                                                                                           gaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGcccc    >  2:6990272/1‑151 (MQ=255)
                                                                                                                                           gaTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGcccc    >  1:1191261/1‑151 (MQ=255)
                                                                                                                                             tctcGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTTGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCTGCCCCGt  >  2:1255778/1‑151 (MQ=255)
                                                                                                                                             tctcGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGt  <  2:4711011/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGCATGCCCTTGCCTTCCGCGCCCATCACCAGCACGGTCGGCCCCGT  >  NC_008463/5809221‑5809512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑