BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	757,697	A→T	Q678L (CAG→CTG)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	757,697	0	A	T	99.5%	1777.6 / NA	416	Q678L (CAG→CTG)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base A (1/0); major base T (179/234); minor base G (1/1); total (181/235)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 416 total aligned reads displayed.

GACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACAA  >  NC_008463/757549‑757844

   tACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTc                                                                                                                                                <  1:923743/151‑1 (MQ=255)

     cATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTcgc                                                                                                                                              >  2:5033862/1‑151 (MQ=255)

            gACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCt                                                                                                                                       <  2:1679946/151‑1 (MQ=255)

                 gATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTcc                                                                                                                                  <  1:10363074/151‑1 (MQ=255)

                  aTGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACTCGGTTTCCGGTAAGGTTATTCGTGCAGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCa                                                                                                                                 <  2:193381/151‑1 (MQ=255)

                    gggCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATg                                                                                                                               <  1:11105860/151‑1 (MQ=255)

                      gCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGa                                                                                                                             <  2:5348457/151‑1 (MQ=255)

                       cGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGaa                                                                                                                            >  2:3240814/1‑151 (MQ=255)

                       cGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGaa                                                                                                                            <  1:5507664/151‑1 (MQ=255)

                          ccTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTCAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATtc                                                                                                                         >  1:570148/1‑151 (MQ=255)

                          ccTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATtc                                                                                                                         >  1:4824278/1‑151 (MQ=255)

                          ccTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATtc                                                                                                                         >  1:8699598/1‑151 (MQ=255)

                          ccTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTGTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATtc                                                                                                                         <  2:7718713/151‑1 (MQ=255)

                             gAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTcc                                                                                                                      >  1:10850153/1‑151 (MQ=255)

                              aaCCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCa                                                                                                                     >  2:9409839/1‑151 (MQ=255)

                               aCCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCaa                                                                                                                    <  1:1205770/151‑1 (MQ=255)

                                ccGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCaaa                                                                                                                   <  2:7973239/151‑1 (MQ=255)

                                    cgtcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACg                                                                                                               >  2:6203445/1‑151 (MQ=255)

                                     gtcgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGc                                                                                                              >  2:10930634/1‑151 (MQ=255)

                                       cgtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCg                                                                                                            <  2:6398390/151‑1 (MQ=255)

                                        gtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGCGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGa                                                                                                           <  2:10677326/151‑1 (MQ=255)

                                        gtGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGa                                                                                                           >  1:3411238/1‑151 (MQ=255)

                                           gTCTGATTCAGGGGATGGTGGACACGGTTTCCTGTAAGGTGATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACAGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGc                                                                                                        <  2:8857187/151‑1 (MQ=255)

                                            tCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCt                                                                                                       >  2:9156993/1‑151 (MQ=255)

                                              tGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTcc                                                                                                     <  1:9643938/151‑1 (MQ=255)

                                               gATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGTAATTCTCCAAATACGCCGAAGCTCCg                                                                                                    >  2:10409021/1‑151 (MQ=255)

                                                aTTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGt                                                                                                   <  1:6226886/151‑1 (MQ=255)

                                                 ttCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTc                                                                                                  <  1:1445350/151‑1 (MQ=255)

                                                     ggggATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAAc                                                                                              <  2:8409361/151‑1 (MQ=255)

                                                         aTGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtcg                                                                                          >  1:7121600/1‑151 (MQ=255)

                                                         aTGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtcg                                                                                          >  2:10162008/1‑151 (MQ=255)

                                                            gaggaCACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtcgtcg                                                                                       >  1:6161444/1‑151 (MQ=255)

                                                            gaggaCACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACAtcgtcg                                                                                       >  1:2856734/1‑151 (MQ=255)

                                                            gaggaCACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCAAGGAATTCTCCAAATACGCCGAAGCTCCGACGAACAtcgtcg                                                                                       >  2:2375403/1‑151 (MQ=255)

                                                             aggaCACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGa                                                                                      <  2:7340237/151‑1 (MQ=255)

                                                                  acGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAgcgc                                                                                 <  2:363598/151‑1 (MQ=255)

                                                                   cGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCt                                                                                <  1:414964/151‑1 (MQ=255)

                                                                    ggTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTg                                                                               <  2:3859040/151‑1 (MQ=255)

                                                                       ttCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGtt                                                                            <  1:8829717/151‑1 (MQ=255)

                                                                       ttCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGtt                                                                            <  1:10679210/151‑1 (MQ=255)

                                                                        tCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGAGGTTCGGTTATGCGACCGACGTGCGTTCCTTGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTa                                                                           <  2:2645053/151‑1 (MQ=255)

                                                                          cGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaa                                                                         >  1:2358915/1‑151 (MQ=255)

                                                                          cGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaa                                                                         >  1:7641290/1‑151 (MQ=255)

                                                                            gTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaaaa                                                                       <  1:7913501/151‑1 (MQ=255)

                                                                             tAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaaaaa                                                                      <  2:8619637/151‑1 (MQ=255)

                                                                             tAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTaaaaaa                                                                      <  1:2085063/151‑1 (MQ=255)

                                                                              aaGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGATCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAAGTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAAc                                                                     >  2:7448186/1‑151 (MQ=255)

                                                                               aGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACa                                                                    <  1:3166122/151‑1 (MQ=255)

                                                                               aGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACa                                                                    <  1:5966659/151‑1 (MQ=255)

                                                                                  ttATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGAAGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAATACAAgg                                                                 >  2:1031084/1‑151 (MQ=255)

                                                                                     ttCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTg                                                              <  2:2289159/151‑1 (MQ=255)

                                                                                      tCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGCGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGa                                                             <  2:7059920/151‑1 (MQ=255)

                                                                                       cGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGTAATTCTCCAAATACGACGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTGAAAAAACAAGGTTGAt                                                            >  2:5859271/1‑151 (MQ=255)

                                                                                         tGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTc                                                          <  2:5267638/151‑1 (MQ=255)

                                                                                         tGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTc                                                          <  2:3452002/151‑1 (MQ=255)

                                                                                          gCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCa                                                         >  2:4692549/1‑151 (MQ=255)

                                                                                          gCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCa                                                         >  2:1290545/1‑151 (MQ=255)

                                                                                            cGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGc                                                       >  1:3060741/1‑151 (MQ=255)

                                                                                            cGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGc                                                       >  2:7148359/1‑151 (MQ=255)

                                                                                               gggtCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACGCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCt                                                    <  2:10588863/148‑1 (MQ=255)

                                                                                               ggTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCt                                                    <  1:409565/151‑1 (MQ=255)

                                                                                               ggTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCt                                                    <  2:6089914/151‑1 (MQ=255)

                                                                                                 ttCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCttt                                                  <  2:1112944/151‑1 (MQ=255)

                                                                                                 ttCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCttt                                                  <  2:3107428/151‑1 (MQ=255)

                                                                                                   ccGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaa                                                <  1:4585255/151‑1 (MQ=255)

                                                                                                     gCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagt                                              >  1:3764517/1‑151 (MQ=255)

                                                                                                      cTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagta                                             <  2:1105629/151‑1 (MQ=255)

                                                                                                       tGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagtaa                                            >  1:1589007/1‑151 (MQ=255)

                                                                                                       tGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagtaa                                            <  2:2985056/151‑1 (MQ=255)

                                                                                                       tGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTtaagtaa                                            <  1:10514810/151‑1 (MQ=255)

                                                                                                           tGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAgg                                        >  1:8455135/1‑151 (MQ=255)

                                                                                                            gagaTGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGt                                       >  2:9053727/1‑151 (MQ=255)

                                                                                                            gagaTGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGt                                       <  2:1367722/151‑1 (MQ=255)

                                                                                                            gagaTGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGt                                       >  1:9398991/1‑151 (MQ=255)

                                                                                                               aTGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTa                                    <  1:8555975/151‑1 (MQ=255)

                                                                                                                 gTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACt                                  <  1:10072342/151‑1 (MQ=255)

                                                                                                                  ttCGGTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTg                                 <  1:4264182/151‑1 (MQ=255)

                                                                                                                      gTTATGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGt                             <  1:9948114/151‑1 (MQ=255)

                                                                                                                          tGCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCt                         >  1:2026498/1‑151 (MQ=255)

                                                                                                                           gCGACCGACGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTa                        <  2:251415/151‑1 (MQ=255)

                                                                                                                              accgacGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAgg                     <  1:9712400/151‑1 (MQ=255)

                                                                                                                              accgacGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAgg                     >  1:5799446/1‑151 (MQ=255)

                                                                                                                               ccgacGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGa                    >  1:1735617/1‑151 (MQ=255)

                                                                                                                                cgacGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGaa                   >  1:2536992/1‑151 (MQ=255)

                                                                                                                                cgacGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGaa                   >  1:6659711/1‑151 (MQ=255)

                                                                                                                                cgacGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGaa                   >  1:8164928/1‑151 (MQ=255)

                                                                                                                                 gacGTGCGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGaaa                  >  2:4166113/1‑151 (MQ=255)

                                                                                                                                 gacGTGCGTTCCATGTCACAGGGTCGCGCGAGCTACTCCATGTAATACTACAAATACGCCGAAGCTCCGTCGAACAACGTCGAATCGCTGGTTAAAAAACAAGGTTGAATCAGCCCTTTAAGAAAGAGGTATACTGTCGTGGCTAATGaaa                  >  2:9696132/1‑151 (MQ=255)

                                                                                                                                      gCGGTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTc             <  2:1513882/151‑1 (MQ=255)

                                                                                                                                       cGTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCg            <  1:6508738/151‑1 (MQ=255)

                                                                                                                                        gTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGa           >  1:3557128/1‑151 (MQ=255)

                                                                                                                                        gTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGa           <  2:5729608/151‑1 (MQ=255)

                                                                                                                                        gTTCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCGAAGGAAAAATTCGa           >  2:549446/1‑151 (MQ=255)

                                                                                                                                         ttCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGaa          >  1:5174526/1‑151 (MQ=255)

                                                                                                                                          tCCATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAATTTCGAAc         >  2:6714421/1‑151 (MQ=255)

                                                                                                                                          tCCATGTCCCGGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATCCGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAAc         <  2:4851883/151‑1 (MQ=255)

                                                                                                                                            cATGTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGt       <  1:7354121/151‑1 (MQ=255)

                                                                                                                                               gTCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAAc    <  1:10923742/151‑1 (MQ=255)

                                                                                                                                               gTCCCGGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAAc    >  2:2117226/1‑151 (MQ=255)

                                                                                                                                                tCCCTGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACa   <  2:2794018/151‑1 (MQ=255)

GACTACATGGGTGACGTGATGGGCGACCTGAACCGTCGTCGTGGTCTGATTCAGGGGATGGAGGACACGGTTTCCGGTAAGGTTATTCGTGCCGAGGTTCCGCTGGGTGAGATGTTCGGTTATGCGACCGACGTGCGTTCCATGTCCCAGGGTCGCGCGAGCTACTCCATGGAATTCTCCAAATACGCCGAAGCTCCGTCGAACATCGTCGAAGCGCTGGTTAAAAAACAAGGTTGATTCAGCCCTTTAAGTAAGAGGTTTACTGTCGTGGCTAAGGAAAAATTCGAACGTAACAA  >  NC_008463/757549‑757844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`