Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 927,917 T→C I56T (ATC→ACC)  PA14_RS04340 → PAS domain S‑box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463927,9170TC99.8% 2234.8 / NA 565I56T (ATC→ACC) PA14_RS04340PAS domain S‑box protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (270/294);  minor base G (0/1);  total (270/295)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 570 total aligned reads displayed.

AAGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCTT  >  NC_008463/927769‑928064
                                                                                                                                                    |                                                                                                                                                   
aaGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGAccc                                                                                                                                                   <  1:5790484/151‑1 (MQ=255)
    tCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACccgccg                                                                                                                                               <  1:8562795/151‑1 (MQ=255)
     cAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCcgccgc                                                                                                                                              >  1:2057614/1‑151 (MQ=255)
      aGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCcgcg                                                                                                                                             >  2:9128942/1‑151 (MQ=255)
       ggCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGt                                                                                                                                            <  1:11003805/151‑1 (MQ=255)
        gCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTg                                                                                                                                           >  1:605257/1‑151 (MQ=255)
        gCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCGGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTg                                                                                                                                           <  1:101661/151‑1 (MQ=255)
         cTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTgg                                                                                                                                          >  2:9503762/1‑151 (MQ=255)
          tGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGa                                                                                                                                         <  2:1798547/151‑1 (MQ=255)
            aGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAAc                                                                                                                                       <  1:10018785/151‑1 (MQ=255)
            aGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAAc                                                                                                                                       >  1:3391985/1‑151 (MQ=255)
              ccGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAActc                                                                                                                                     <  1:7682723/151‑1 (MQ=255)
                gCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTa                                                                                                                                   <  1:1359376/151‑1 (MQ=255)
                gCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTa                                                                                                                                   >  1:6544944/1‑151 (MQ=255)
                gCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTa                                                                                                                                   >  2:2653491/1‑151 (MQ=255)
                 cTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTAc                                                                                                                                  <  1:4865533/151‑1 (MQ=255)
                   gggcgggcGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCa                                                                                                                                >  1:8333617/1‑151 (MQ=255)
                   gggcgggcGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCa                                                                                                                                <  2:8561708/151‑1 (MQ=255)
                   gggcgggcGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCa                                                                                                                                <  2:286927/151‑1 (MQ=255)
                      cgggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTa                                                                                                                             <  1:6378386/151‑1 (MQ=255)
                      cgggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTa                                                                                                                             >  1:1589131/1‑151 (MQ=255)
                        ggcgCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATg                                                                                                                           <  2:670243/151‑1 (MQ=255)
                        ggcgCGACACTGGCGCTCGGACTGGTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATg                                                                                                                           >  1:5221205/1‑151 (MQ=255)
                          cgcgACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGc                                                                                                                         <  1:3949183/151‑1 (MQ=255)
                          cgcgACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGc                                                                                                                         <  1:7872448/151‑1 (MQ=255)
                           gcgTCACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCt                                                                                                                        >  2:3781982/1‑151 (MQ=255)
                            cgACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCtt                                                                                                                       <  1:4302775/151‑1 (MQ=255)
                               cacTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCg                                                                                                                    >  2:7097040/1‑151 (MQ=255)
                                 cTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGACCTCTACCAGTATGGCTTGCGTg                                                                                                                  >  2:10373784/1‑151 (MQ=255)
                                 cTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTg                                                                                                                  >  1:2393458/1‑151 (MQ=255)
                                   ggCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTggg                                                                                                                <  2:7794295/151‑1 (MQ=255)
                                     cgcTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGt                                                                                                              <  1:2707627/151‑1 (MQ=255)
                                     cgcTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGt                                                                                                              <  1:9539051/151‑1 (MQ=255)
                                      gcTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTg                                                                                                             <  2:5498088/151‑1 (MQ=255)
                                       cTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgc                                                                                                            >  1:7186721/1‑151 (MQ=255)
                                        tCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgcg                                                                                                           <  1:11193033/151‑1 (MQ=255)
                                        tCGGACTGTTGCTCTCCGGGCTGGGCGCCTGCGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgcg                                                                                                           <  2:10892231/151‑1 (MQ=255)
                                         cGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgcgc                                                                                                          >  2:9801561/1‑151 (MQ=255)
                                         cGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgcgc                                                                                                          <  2:6858378/151‑1 (MQ=255)
                                         cGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTgcgc                                                                                                          >  1:3645897/1‑151 (MQ=255)
                                           gACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCgg                                                                                                        <  2:5292701/151‑1 (MQ=255)
                                                 tGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGACCTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCtt                                                                                                  >  2:1317244/1‑151 (MQ=255)
                                                      tCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGa                                                                                             >  2:1645929/1‑151 (MQ=255)
                                                       ccGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGAc                                                                                            <  2:8944281/151‑1 (MQ=255)
                                                       ccGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGAc                                                                                            <  2:7415563/151‑1 (MQ=255)
                                                       ccGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGAc                                                                                            >  1:7603292/1‑151 (MQ=255)
                                                        cGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACa                                                                                           >  2:2425908/1‑151 (MQ=255)
                                                         gggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAg                                                                                          <  2:8075690/151‑1 (MQ=255)
                                                          ggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGc                                                                                         <  1:8802630/151‑1 (MQ=255)
                                                          ggCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGGGCCTTGCTGACAGc                                                                                         <  2:3939547/151‑1 (MQ=255)
                                                             tGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCggg                                                                                      >  2:8164979/1‑151 (MQ=255)
                                                             tGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCggg                                                                                      >  2:628149/1‑151 (MQ=255)
                                                              gggCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGc                                                                                     <  2:9265854/151‑1 (MQ=255)
                                                                  gcCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAgg                                                                                 <  1:9651650/151‑1 (MQ=255)
                                                                   ccTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgc                                                                                <  2:8292819/151‑1 (MQ=255)
                                                                    cTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGgcg                                                                               >  1:7425542/1‑151 (MQ=255)
                                                                       ggggCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGcac                                                                            >  2:1513498/1‑151 (MQ=255)
                                                                              gcgcTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGAcc                                                                     <  2:6556091/151‑1 (MQ=255)
                                                                                gcTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcgc                                                                   >  2:4771452/1‑151 (MQ=255)
                                                                                gcTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACcgc                                                                   <  1:8935461/151‑1 (MQ=255)
                                                                                   ggTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGaa                                                                >  2:4199988/1‑151 (MQ=255)
                                                                                       aaCGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGt                                                            <  2:7985777/151‑1 (MQ=255)
                                                                                        aCGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGtt                                                           <  2:10574938/151‑1 (MQ=255)
                                                                                        aCGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGtt                                                           >  2:7325424/1‑151 (MQ=255)
                                                                                           aGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccg                                                        >  1:10868401/1‑151 (MQ=255)
                                                                                              aaCAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGAGTGGAACTCTACCATTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTccgccg                                                     <  2:847193/151‑1 (MQ=255)
                                                                                                  ggCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTAc                                                 >  1:6904170/1‑151 (MQ=255)
                                                                                                   gcgcGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACa                                                <  1:4562094/151‑1 (MQ=255)
                                                                                                   gcgcGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACa                                                <  2:4911766/151‑1 (MQ=255)
                                                                                                     gcgGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGc                                              >  2:10055623/1‑151 (MQ=255)
                                                                                                        gATGGCGCTGGAACGCGAGGGGGTCCTGCTGGCCGAGGCGGTGAGCCGCAGCGTGGAACTCTACCAGTATGGGTTGCGTGGGTTGCGCGTTGCCTTGCTGACAGCGGGCGAGGCGATCATCTACCGCGAACTGTTCCGCCGCTACAGCCTg                                           <  2:3391801/151‑1 (MQ=255)
                                                                                                        gATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTg                                           <  1:7014844/151‑1 (MQ=255)
                                                                                                            gcgcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGAccc                                       >  2:4454570/1‑151 (MQ=255)
                                                                                                              gcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCcgc                                     >  2:761153/1‑151 (MQ=255)
                                                                                                              gcTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCcgc                                     >  1:11164150/1‑151 (MQ=255)
                                                                                                                 ggAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGAc                                  <  1:3492500/151‑1 (MQ=255)
                                                                                                                  gAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACa                                 >  2:8663523/1‑151 (MQ=255)
                                                                                                                    aCGCGAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATc                               <  1:9197303/151‑1 (MQ=255)
                                                                                                                     cgcgAGGCGGAACTGCTGGCCGCGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCGGACCCGCGACATCg                              <  1:10344851/151‑1 (MQ=255)
                                                                                                                     cgcgAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCg                              >  1:8165799/1‑151 (MQ=255)
                                                                                                                        gAGGCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGAcc                           <  1:10499810/151‑1 (MQ=255)
                                                                                                                          ggCGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTTCCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcgc                         <  2:5135935/151‑1 (MQ=255)
                                                                                                                           gCGGAACTGCGGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACcgcg                        <  2:6021051/151‑1 (MQ=255)
                                                                                                                            cGGAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGa                       >  2:5728577/1‑151 (MQ=255)
                                                                                                                             ggAACTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAg                      >  1:2918812/1‑151 (MQ=255)
                                                                                                                              gAAGTGCTGGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCGTGCTTGGGGTGCGCGCGGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGt                     <  1:3214293/151‑1 (MQ=255)
                                                                                                                                 ctgctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTc                  <  1:6244290/151‑1 (MQ=255)
                                                                                                                                   gctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTccc                <  2:8776556/151‑1 (MQ=255)
                                                                                                                                    ctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCg               <  2:11156454/151‑1 (MQ=255)
                                                                                                                                    ctgGCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCg               <  2:3212680/151‑1 (MQ=255)
                                                                                                                                      ggCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGgc             <  2:2204179/151‑1 (MQ=255)
                                                                                                                                       gCCGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGgcg            >  1:4433700/1‑151 (MQ=255)
                                                                                                                                         cGAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGcc          <  1:1794030/151‑1 (MQ=255)
                                                                                                                                          gAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGccc         >  1:9739499/1‑151 (MQ=255)
                                                                                                                                          gAGGCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGccc         >  1:538893/1‑151 (MQ=255)
                                                                                                                                             gCGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCcgcg      >  1:555936/1‑151 (MQ=255)
                                                                                                                                              cGGTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcgg     <  2:5783720/151‑1 (MQ=255)
                                                                                                                                               ggTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggc    >  1:5062302/1‑151 (MQ=255)
                                                                                                                                                gTGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggct   <  2:1405605/151‑1 (MQ=255)
                                                                                                                                                 tGACCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGcggctt  <  2:6202649/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGTTCAGGCTGAGCCGCTGGGCGGGCGCGACACTGGCGCTCGGACTGTTGCTGTCCGGGCTGGGCGCCTGGGGGCTGGCGCTGGTCAACGAGCAACAGGCGCGGATGGCGCTGGAACGCGAGGCGGAACTGCTGGCCGAGGCGGTGATCCGCCGCGTGGAACTCTACCAGTATGGCTTGCGTGGGGTGCGCGGTGCCTTGCTGACAGCGGGCGAGGCGCACATCGACCGCGAACTGTTCCGCCGCTACAGCCTGACCCGCGACATCGACCGCGAGTTTCCCGGCGCCCGCGGCTT  >  NC_008463/927769‑928064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑