Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 982,940 A→G F293F (TTT→TTC)  PA14_RS04570 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463982,9400AG99.6% 1943.3 / NA 494F293F (TTT→TTC) PA14_RS04570ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (240/252);  minor base T (0/2);  total (240/254)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 500 total aligned reads displayed.

CCTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAGG  >  NC_008463/982792‑983087
                                                                                                                                                    |                                                                                                                                                   
ccTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACgaa                                                                                                                                                   <  1:6640534/151‑1 (MQ=255)
 cTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAAc                                                                                                                                                  <  1:8101342/151‑1 (MQ=255)
  tCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACa                                                                                                                                                 <  1:11098339/151‑1 (MQ=255)
  tCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACa                                                                                                                                                 <  1:9309704/151‑1 (MQ=255)
    ggCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGt                                                                                                                                               >  2:9165588/1‑151 (MQ=255)
    ggCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGt                                                                                                                                               <  1:4934773/151‑1 (MQ=255)
    ggCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCG‑CGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTg                                                                                                                                              >  2:3600794/1‑151 (MQ=255)
     gCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTg                                                                                                                                              <  2:5344918/151‑1 (MQ=255)
     gCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTg                                                                                                                                              >  1:3134113/1‑151 (MQ=255)
       cagcagcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGcc                                                                                                                                            <  1:1708231/151‑1 (MQ=255)
         gcagcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCtt                                                                                                                                          <  2:5866839/151‑1 (MQ=255)
          cagcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtc                                                                                                                                         >  1:8483143/1‑151 (MQ=255)
           agcagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTtct                                                                                                                                        <  1:8224063/151‑1 (MQ=255)
             cagGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcg                                                                                                                                      <  1:6168242/151‑1 (MQ=255)
                gCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcggcg                                                                                                                                   >  2:10254442/1‑151 (MQ=255)
                gCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcggcg                                                                                                                                   <  2:8086143/151‑1 (MQ=255)
                 ccGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcggcgg                                                                                                                                  >  2:1409921/1‑151 (MQ=255)
                 ccGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTcggcgg                                                                                                                                  >  1:10683501/1‑151 (MQ=255)
                  cGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGt                                                                                                                                 >  2:6975348/1‑151 (MQ=255)
                  cGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGt                                                                                                                                 <  2:10533003/151‑1 (MQ=255)
                  cGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGt                                                                                                                                 <  1:8978682/151‑1 (MQ=255)
                   gAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTg                                                                                                                                >  1:6018392/1‑151 (MQ=255)
                   gAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTg                                                                                                                                <  2:7125159/151‑1 (MQ=255)
                     aGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGcc                                                                                                                              >  1:4595008/1‑151 (MQ=255)
                         gtgCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATc                                                                                                                          >  2:10986799/1‑151 (MQ=255)
                         gtgCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATc                                                                                                                          >  2:10723953/1‑151 (MQ=255)
                           gCCAGGGGCGCGCGCCCACCGAGCGCAGGCTGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAg                                                                                                                        <  2:9411494/151‑1 (MQ=255)
                                   cgcgcgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCcaca                                                                                                                <  1:7782080/151‑1 (MQ=255)
                                       cgcCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTc                                                                                                            >  1:1553410/1‑151 (MQ=255)
                                         cccACCGCGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCt                                                                                                          <  2:1190768/151‑1 (MQ=255)
                                             ccGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAg                                                                                                      <  1:2884269/151‑1 (MQ=255)
                                               gggcgcAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAggg                                                                                                    >  1:7436407/3‑151 (MQ=255)
                                               gAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAggg                                                                                                    <  1:3432121/151‑1 (MQ=255)
                                                 gcgcAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGATCAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCTGGGCg                                                                                                  <  2:8364136/151‑1 (MQ=255)
                                                  cgcAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGAACAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGa                                                                                                 >  2:2114940/1‑151 (MQ=255)
                                                   gcAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGac                                                                                                >  1:5546960/1‑151 (MQ=255)
                                                    cAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGaca                                                                                               <  1:2586012/151‑1 (MQ=255)
                                                    cAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGAGCAGGCCGGGGAGGACGACGAACAGCGGGACCACGACCAGTGCCTTCTCGGCGGTGCCCCTCAGGCCCCACAGTGCCTGCAGGGCGaca                                                                                               >  1:3823424/1‑151 (MQ=255)
                                                      ggATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACAcc                                                                                             >  1:8628508/1‑151 (MQ=255)
                                                          ggCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCgggg                                                                                         >  1:9696159/1‑151 (MQ=255)
                                                          ggCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCgggg                                                                                         <  1:10032471/151‑1 (MQ=255)
                                                          ggCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCgggg                                                                                         <  2:3201436/151‑1 (MQ=255)
                                                          ggCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCgggg                                                                                         >  2:6709886/1‑151 (MQ=255)
                                                            ccATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGggag                                                                                       >  2:6406197/1‑151 (MQ=255)
                                                               tctcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGAt                                                                                    <  2:3023366/151‑1 (MQ=255)
                                                               tctcCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGAt                                                                                    >  2:4732659/1‑151 (MQ=255)
                                                                   ccGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCCTGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGcc                                                                                <  2:2455249/151‑1 (MQ=255)
                                                                      gcggcgCTCGTTGAGGCTGGTGAGGATCGCCGAGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATc                                                                             <  2:4139965/151‑1 (MQ=255)
                                                                         gcgcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGc                                                                          <  2:10094251/151‑1 (MQ=255)
                                                                           gcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCgg                                                                        >  2:4853241/1‑151 (MQ=255)
                                                                           gcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCgg                                                                        >  2:9944151/1‑151 (MQ=255)
                                                                           gcTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCgg                                                                        >  1:1175898/1‑151 (MQ=255)
                                                                            cTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGt                                                                       >  1:7998764/1‑151 (MQ=255)
                                                                             tCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGtt                                                                      <  1:587152/151‑1 (MQ=255)
                                                                               gTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCg                                                                    >  1:2208518/1‑151 (MQ=255)
                                                                               gTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCg                                                                    >  1:7010738/1‑151 (MQ=255)
                                                                               gTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCg                                                                    >  1:199774/1‑151 (MQ=255)
                                                                                 tGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGcc                                                                  >  1:7845024/1‑151 (MQ=255)
                                                                                  gAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCg                                                                 <  1:3319440/151‑1 (MQ=255)
                                                                                     gCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGa                                                              >  2:2213715/1‑151 (MQ=255)
                                                                                      cTGGTGAGGATCGCCGTGAGCATGCCGATCTGGCCGGTGAGGACGACTAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGAt                                                             <  1:7600484/151‑1 (MQ=255)
                                                                                      cTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGGTCCTGCAGGGCGACACCGGGGAGGATCGCCAGCGGCGGTTCGCCGGGAt                                                             >  2:4994996/1‑151 (MQ=255)
                                                                                          cgAGGCTCGCCGTGAGCTTGCCGATCCGGCGCGTGCGGACGACGACCTGGGAGAGCCCGCACGGGGGCTTCGCGGCGGTGCGCATCAGGCCCCTCAGTTCCTGGCGGGCGTCACCGGGGTGGATCGCCTTCAGCGGTTCGCCGGGATAGTc                                                         <  2:6831305/150‑1 (MQ=255)
                                                                                              gATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTg                                                     >  2:849479/1‑151 (MQ=255)
                                                                                                 cGCCGTGAGCATGCCGCTCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGAcc                                                  <  2:372591/151‑1 (MQ=255)
                                                                                                  gCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCt                                                 >  2:6025486/1‑151 (MQ=255)
                                                                                                   ccGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTc                                                >  2:5130474/1‑151 (MQ=255)
                                                                                                   ccGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTc                                                >  1:9842854/1‑151 (MQ=255)
                                                                                                     gTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcgc                                              >  2:2793164/1‑151 (MQ=255)
                                                                                                      tGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTcgcg                                             >  1:5417555/1‑151 (MQ=255)
                                                                                                       gAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGt                                            <  2:3505126/151‑1 (MQ=255)
                                                                                                         gCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTg                                          <  1:4471137/151‑1 (MQ=255)
                                                                                                          caggCCGATCAGGCCGGTGAGGACGACGAACAGCGATACCACTAACAGTGCCTTCTCGGCGGTGCCCTTCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGc                                         <  2:8918849/148‑1 (MQ=255)
                                                                                                            tGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAg                                       <  1:9193052/151‑1 (MQ=255)
                                                                                                             gCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAgg                                      >  2:11160768/1‑151 (MQ=255)
                                                                                                              ccGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAggg                                     <  2:9857719/151‑1 (MQ=255)
                                                                                                               cGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGc                                    <  2:10824172/151‑1 (MQ=255)
                                                                                                                gATCAGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCg                                   <  1:3599903/151‑1 (MQ=255)
                                                                                                                    aGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAgg                               >  2:7961510/1‑151 (MQ=255)
                                                                                                                    aGGCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAgg                               >  2:1765474/1‑151 (MQ=255)
                                                                                                                     ggCCGGTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGt                              <  1:4157514/151‑1 (MQ=255)
                                                                                                                         ggTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGcc                          >  1:10494459/1‑151 (MQ=255)
                                                                                                                         ggTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGcc                          >  2:9520847/1‑151 (MQ=255)
                                                                                                                         ggTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGcc                          >  1:5120232/1‑151 (MQ=255)
                                                                                                                          gTGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCa                         <  2:1642660/151‑1 (MQ=255)
                                                                                                                           tGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCAc                        <  1:3939616/151‑1 (MQ=255)
                                                                                                                           tGAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCAc                        <  1:3112708/151‑1 (MQ=255)
                                                                                                                            gAGGACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCAcc                       >  2:7395382/1‑151 (MQ=255)
                                                                                                                              ggACGACGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCtt                     <  2:187772/151‑1 (MQ=255)
                                                                                                                               gacgacGAACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCGCAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTGCGCCGGGATAGTCGTGGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTg                    >  2:3409493/1‑151 (MQ=255)
                                                                                                                                 cgacgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGct                  >  2:4468623/1‑151 (MQ=255)
                                                                                                                                 cgacgaACAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGcg                  >  2:780634/1‑150 (MQ=255)
                                                                                                                                        cAGCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAgg           <  2:1574125/151‑1 (MQ=255)
                                                                                                                                          gCGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGcc         <  1:6811710/151‑1 (MQ=255)
                                                                                                                                           cGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCg        <  1:7170063/151‑1 (MQ=255)
                                                                                                                                           cGAGACCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCg        >  2:9260448/1‑151 (MQ=255)
                                                                                                                                            gagaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCCTCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGa       >  2:5675111/1‑151 (MQ=255)
                                                                                                                                            gagaCCACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGa       >  2:5501872/1‑151 (MQ=255)
                                                                                                                                                ccACGAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAg   <  1:2464055/151‑1 (MQ=255)
                                                                                                                                                 cacgAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAgg  >  1:2717050/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCTCGGCCAGCAGCAGGCCGAAGATGTGCCAGGGGCGCGCGCCCACCGAGCGCAGGATGGCCATCTCCCGGCGGCGCTCGTTGAGGCTGGTGAGGATCGCCGTGAGCATGCCGATCAGGCCGGTGAGGACGACGAACAGCGAGACCACAAACAGTGCCTTCTCGGCGGTGCCCATCAGGCCCCACAGTTCCTGCAGGGCGACACCGGGGAGGATCGCCATCAGCGGTTCGCCGGGATAGTCGTTGACCTCGCGTTGCAGGGCGAAGGTCGCCACCTTGCTCTTCAGGCCGACCAGG  >  NC_008463/982792‑983087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑