Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 791,862 3 bp→GCT coding (1842‑1844/5430 nt) pchF → pyochelin non‑ribosomal peptide synthetase PchF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463791,8620CG99.6% 1900.3 / ‑2.1 487A614A (GCC→GCG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (306/179);  total (308/179)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
*NC_008463791,8630TC99.6% 1940.1 / ‑1.8 487W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (306/179);  total (308/179)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
*NC_008463791,8640GT98.6% 2019.6 / NA 487W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base T (304/175);  total (308/179)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 487 total aligned reads displayed.

TCGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCGGCT  >  NC_008463/791720‑792007
                                                                                                                                              |||                                                                                                                                               
tCGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTg                                                                                                                                           <  2:7611979/151‑1 (MQ=255)
 cgcgGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGc                                                                                                                                          >  1:6302754/1‑151 (MQ=255)
 cgcgGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGc                                                                                                                                          >  2:6960416/1‑151 (MQ=255)
   cgGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCt                                                                                                                                        >  2:3998621/1‑151 (MQ=255)
    ggTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTg                                                                                                                                       >  2:7351545/1‑151 (MQ=255)
     gTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTgg                                                                                                                                      >  2:7764373/1‑151 (MQ=255)
        aTGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGaaa                                                                                                                                   <  2:5194144/151‑1 (MQ=255)
           ctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCg                                                                                                                                <  1:4348606/151‑1 (MQ=255)
            tgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGa                                                                                                                               <  2:1896117/151‑1 (MQ=255)
              ccGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATg                                                                                                                             >  1:6625288/1‑151 (MQ=255)
               cGAAAAGCGCCGCGCACTTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGt                                                                                                                            <  2:6173630/151‑1 (MQ=255)
                  aaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTccc                                                                                                                         <  1:5937988/151‑1 (MQ=255)
                  aaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTccc                                                                                                                         <  1:5096213/151‑1 (MQ=255)
                   aaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGCTGTCCCg                                                                                                                        >  2:9520366/1‑151 (MQ=255)
                   aaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCg                                                                                                                        >  2:2996784/1‑151 (MQ=255)
                   aaGCGCCGCGCAACTGGTCGCGGTGCTCGGCATCCTCCAGGCCGGCGCCGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCg                                                                                                                        <  1:6856174/151‑1 (MQ=255)
                     gcgcCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCgga                                                                                                                      >  2:1188247/1‑149 (MQ=255)
                      cgccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCAGCGGCGCCAGGCGATCCTCGCCAGCGCCGATGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGAc                                                                                                                     <  2:10169042/151‑1 (MQ=255)
                         cgcgCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGcc                                                                                                                  <  1:3096304/151‑1 (MQ=255)
                          gcgcAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCg                                                                                                                 <  2:11141376/151‑1 (MQ=255)
                             cAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCt                                                                                                              <  1:9174161/151‑1 (MQ=255)
                             cAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCt                                                                                                              <  2:8534906/151‑1 (MQ=255)
                                 tGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGACAGCGATGTCCCGGACGCCGGCTgcgc                                                                                                          >  1:146126/1‑151 (MQ=255)
                                     cgcgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGACAGCGATGTCCCGGACGCCGGCTGCGCCTGc                                                                                                      >  2:10581567/1‑151 (MQ=255)
                                      gcgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCg                                                                                                     >  1:4826194/1‑151 (MQ=255)
                                          tGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGc                                                                                                 <  1:278261/151‑1 (MQ=255)
                                            aTCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCa                                                                                               >  2:4139710/1‑151 (MQ=255)
                                               ggCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGACAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCg                                                                                            >  1:10857104/1‑151 (MQ=255)
                                               ggCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCg                                                                                            >  1:2972243/1‑151 (MQ=255)
                                                gCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGa                                                                                           <  1:6399367/151‑1 (MQ=255)
                                                 cATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAc                                                                                          >  2:4615166/1‑151 (MQ=255)
                                                  aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAcc                                                                                         >  1:2287682/1‑151 (MQ=255)
                                                  aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAcc                                                                                         <  1:3193118/151‑1 (MQ=255)
                                                  aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAcc                                                                                         <  2:5834426/151‑1 (MQ=255)
                                                   tcctccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCg                                                                                        >  2:1963393/1‑151 (MQ=255)
                                                     ctccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGc                                                                                      >  2:7452522/1‑151 (MQ=255)
                                                       ccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTg                                                                                    <  1:9699746/151‑1 (MQ=255)
                                                       ccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTg                                                                                    >  2:9919603/1‑151 (MQ=255)
                                                         aGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgc                                                                                  <  1:3431008/151‑1 (MQ=255)
                                                         aGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgc                                                                                  >  2:8069112/1‑151 (MQ=255)
                                                            ccGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgc                                                                               >  2:3104679/1‑151 (MQ=255)
                                                             cGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgcc                                                                              <  1:4964306/151‑1 (MQ=255)
                                                             cGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgcc                                                                              >  1:536034/1‑151 (MQ=255)
                                                                cgcAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGAc                                                                           <  2:2223864/151‑1 (MQ=255)
                                                                 gcAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACa                                                                          >  2:2532066/1‑151 (MQ=255)
                                                                 gcAGCCTAGGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACa                                                                          <  2:10367166/151‑1 (MQ=255)
                                                                    gCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAgcg                                                                       <  1:4082441/151‑1 (MQ=255)
                                                                     ccTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAgcgc                                                                      >  1:3545357/1‑151 (MQ=255)
                                                                     ccTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAgcgc                                                                      >  1:8585542/1‑151 (MQ=255)
                                                                       tATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCt                                                                    >  2:242565/1‑151 (MQ=255)
                                                                        aTGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCTATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTg                                                                   <  2:7196525/151‑1 (MQ=255)
                                                                        aTGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTg                                                                   >  1:9934825/1‑151 (MQ=255)
                                                                         tgtgCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTgg                                                                  >  1:8833160/1‑151 (MQ=255)
                                                                            gCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgccg                                                               >  1:2557739/1‑151 (MQ=255)
                                                                              cGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgccgcc                                                             >  2:9024762/1‑151 (MQ=255)
                                                                                 tGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAcc                                                          >  2:3354990/1‑151 (MQ=255)
                                                                                 tGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAcc                                                          >  1:10385190/1‑151 (MQ=255)
                                                                                  ggACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccg                                                         <  2:9272413/151‑1 (MQ=255)
                                                                                   gACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgc                                                        >  1:7552942/1‑151 (MQ=255)
                                                                                      aTCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGTCCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcccg                                                     >  2:6649952/1‑151 (MQ=255)
                                                                                        ccGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCcgcg                                                   >  2:35772/1‑151 (MQ=255)
                                                                                          gCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCGCTCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcggc                                                 >  1:5721005/1‑151 (MQ=255)
                                                                                          gCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcggc                                                 >  1:9002057/1‑151 (MQ=255)
                                                                                            cAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGcggcgg                                               <  1:11102263/151‑1 (MQ=255)
                                                                                             aGCCGCCGCTGCGGCGCCAGGCGAGCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGa                                              >  1:1014975/1‑151 (MQ=255)
                                                                                                cgccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGt                                           >  1:8116363/1‑151 (MQ=255)
                                                                                                  ccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTgg                                         <  1:4128748/151‑1 (MQ=255)
                                                                                                  ccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTgg                                         >  2:6436648/1‑151 (MQ=255)
                                                                                                   cgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggc                                        <  2:546577/151‑1 (MQ=255)
                                                                                                    gcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcg                                       <  1:1760819/151‑1 (MQ=255)
                                                                                                    gcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcg                                       >  1:7433623/1‑151 (MQ=255)
                                                                                                     cTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcgg                                      <  1:10731258/151‑1 (MQ=255)
                                                                                                       gcggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGgcggcg                                    <  2:571972/151‑1 (MQ=255)
                                                                                                         ggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGga                                  >  1:1338756/1‑151 (MQ=255)
                                                                                                         ggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGga                                  >  1:4381551/1‑151 (MQ=255)
                                                                                                         ggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGga                                  >  2:11067876/1‑151 (MQ=255)
                                                                                                              cAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGAcc                             >  2:6508387/1‑151 (MQ=255)
                                                                                                              cAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGAcc                             >  1:10501275/1‑151 (MQ=255)
                                                                                                                ggCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTc                           >  1:7143012/1‑151 (MQ=255)
                                                                                                                ggCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTc                           >  2:5917566/1‑151 (MQ=255)
                                                                                                                ggCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTc                           >  2:7868239/1‑151 (MQ=255)
                                                                                                                  cGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGc                         >  2:3768861/1‑151 (MQ=255)
                                                                                                                  cGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCAGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCAGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGc                         >  1:8675100/1‑151 (MQ=255)
                                                                                                                   gATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGcc                        >  2:2936115/1‑151 (MQ=255)
                                                                                                                    aTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCt                       >  2:8812261/1‑151 (MQ=255)
                                                                                                                    aTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCt                       <  2:6934534/151‑1 (MQ=255)
                                                                                                                     tCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGAGGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTa                      <  1:786639/151‑1 (MQ=255)
                                                                                                                      ccTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTAc                     <  1:6171330/151‑1 (MQ=255)
                                                                                                                       cTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACg                    >  1:1901151/1‑151 (MQ=255)
                                                                                                                            cAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATc               >  2:4325819/1‑151 (MQ=255)
                                                                                                                             aGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCt              <  2:4961637/151‑1 (MQ=255)
                                                                                                                              gcgccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTa             <  1:9101160/148‑1 (MQ=255)
                                                                                                                               cgccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTac            >  2:4767863/3‑151 (MQ=255)
                                                                                                                               cgccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTac            >  1:3965103/3‑151 (MQ=255)
                                                                                                                               cgccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTac            >  1:9593109/3‑151 (MQ=255)
                                                                                                                                 ccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTacac          >  2:2625612/1‑151 (MQ=255)
                                                                                                                                  cGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGCTCTACAcc         >  2:1619709/1‑151 (MQ=255)
                                                                                                                                   gAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCt        <  1:5611576/151‑1 (MQ=255)
                                                                                                                                   gAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCt        >  2:9763113/1‑151 (MQ=255)
                                                                                                                                         gTCGCGCGGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAGCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCGGCt  <  1:9762181/151‑1 (MQ=255)
                                                                                                                                              |||                                                                                                                                               
TCGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCGGCT  >  NC_008463/791720‑792007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑