BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,670,537	Δ1 bp	coding (537/1020 nt)	PA14_RS16675 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,670,534	0	T	.	98.5%	1890.4 / 20.9	394	coding (534/1020 nt)	PA14_RS16675	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (5/1); new base . (174/213); total (180/214)
Fisher's exact test for biased strand distribution p-value = 9.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 418 total aligned reads displayed.

GCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTC  >  NC_008463/3670385‑3670678

gCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGt                                                                                                                                                   <  1:9755478/150‑1 (MQ=255)

  ccGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGttt                                                                                                                                                 >  2:765707/1‑150 (MQ=255)

    gcgACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGtttcc                                                                                                                                               <  2:3231374/150‑3 (MQ=255)

      gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGtttcctg                                                                                                                                             >  1:8183461/1‑146 (MQ=255)

        cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                         <  2:5055702/150‑1 (MQ=255)

            cTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagtc                                                                                                                                     <  2:6660829/150‑5 (MQ=255)

             tCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagtct                                                                                                                                    <  1:6257563/150‑6 (MQ=255)

              cGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTg                                                                                                                                    >  2:3977607/1‑150 (MQ=255)

                  cGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCg                                                                                                                                <  1:6898647/150‑1 (MQ=255)

                   gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgg                                                                                                                               >  2:3846804/1‑150 (MQ=255)

                   gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgg                                                                                                                               >  1:3968666/1‑150 (MQ=255)

                     gTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgggg                                                                                                                             <  1:1162503/150‑1 (MQ=255)

                      tGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGCCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGc                                                                                                                            <  1:4314564/150‑1 (MQ=255)

                        aTTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTg                                                                                                                          >  2:1436089/1‑150 (MQ=255)

                        aTTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTg                                                                                                                          >  1:1084493/1‑150 (MQ=255)

                         tttACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGTTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGc                                                                                                                         >  1:7325367/1‑150 (MQ=255)

                            aCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCgg                                                                                                                      <  2:7896906/150‑1 (MQ=255)

                            aCGGCTTCCGCGTGTCGATCCTCGTCGCCCGGACCCTGACCGTGCTCAGTTCTCGGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTCCGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCgg                                                                                                                      <  2:5621882/150‑1 (MQ=255)

                                cTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGct                                                                                                                  <  1:10184558/150‑1 (MQ=255)

                                 atCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCCAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGctc                                                                                                                 <  2:4752690/149‑1 (MQ=38)

                                  tCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGctct                                                                                                                <  1:8772260/150‑1 (MQ=255)

                                   ccGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTa                                                                                                               >  1:2580097/1‑150 (MQ=255)

                                    cgcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTAc                                                                                                              >  2:7800123/1‑150 (MQ=255)

                                      cgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACct                                                                                                            >  2:9604970/1‑150 (MQ=255)

                                        tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                          <  2:6443475/150‑1 (MQ=255)

                                         gtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                         <  2:4919396/150‑1 (MQ=255)

                                         gtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                         >  2:7373566/1‑150 (MQ=255)

                                          tCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgctg                                                                                                        >  1:6690419/1‑150 (MQ=255)

                                            gATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGat                                                                                                      >  1:11161877/1‑150 (MQ=255)

                                             aTCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatc                                                                                                     <  2:575559/150‑1 (MQ=255)

                                              tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatca                                                                                                    <  2:651854/150‑1 (MQ=255)

                                               ccTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcat                                                                                                   <  2:2258127/150‑1 (MQ=255)

                                                ctctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCGGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcatc                                                                                                  >  2:6584734/1‑150 (MQ=255)

                                                 tctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATcc                                                                                                 >  1:3376366/1‑150 (MQ=255)

                                                  ctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTAGTACGGCGGCTGGGTCGACCTGCTCGGCCAG‑GGGTTCGTGGAAGTCTGGTCGGGGCGGCCGGTGCTCTACCTGCTGATCATccg                                                                                                >  2:7490150/1‑149 (MQ=255)

                                                  cgtCGCCCTGACCCGGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCt                                                                                                <  2:6786661/148‑1 (MQ=255)

                                                    tCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTgg                                                                                              >  1:2406837/1‑150 (MQ=255)

                                                    tCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTgg                                                                                              >  1:8989361/1‑150 (MQ=255)

                                                     cGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTggt                                                                                             <  2:10282677/150‑2 (MQ=255)

                                                       ccctgaccctgaCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCa                                                                                           >  1:854237/1‑150 (MQ=255)

                                                        cctgaccctgacCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                          <  2:2456334/150‑1 (MQ=255)

                                                        cctgaccctgacCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                          <  1:10399788/150‑1 (MQ=255)

                                                         ctgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGc                                                                                         >  1:9826352/1‑150 (MQ=255)

                                                           gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                       >  2:8985521/1‑150 (MQ=255)

                                                             ccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCg                                                                                     <  1:7148095/150‑1 (MQ=255)

                                                               ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTc                                                                                   >  1:9469850/1‑150 (MQ=255)

                                                               ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTc                                                                                   <  1:1373969/150‑1 (MQ=255)

                                                                tgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTcc                                                                                  <  1:1278457/150‑1 (MQ=255)

                                                                 gaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTCCTGGG‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCa                                                                                 >  2:2713929/1‑150 (MQ=255)

                                                                 gaccGTGCTCAGTTCTCTGAGCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCa                                                                                 <  2:10873441/150‑1 (MQ=255)

                                                                    cGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGcc                                                                              <  2:2393774/150‑1 (MQ=255)

                                                                      tGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TGTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGa                                                                            <  2:7859756/150‑1 (MQ=255)

                                                                        cTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAAc                                                                          <  2:8603512/150‑1 (MQ=255)

                                                                        cTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAAc                                                                          <  1:6352102/150‑1 (MQ=255)

                                                                          cAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACtt                                                                        <  2:10595366/150‑1 (MQ=255)

                                                                           aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGTTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTc                                                                       >  1:5800832/1‑150 (MQ=255)

                                                                           aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTc                                                                       <  2:8053035/150‑1 (MQ=255)

                                                                             ttCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtg                                                                     >  2:9421796/1‑150 (MQ=255)

                                                                              tctcTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtgg                                                                    >  1:8365112/1‑150 (MQ=255)

                                                                                  tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgc                                                                <  1:5285919/150‑1 (MQ=255)

                                                                                  tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGATGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgc                                                                <  2:5971955/150‑1 (MQ=255)

                                                                                    aTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctg                                                              >  1:7574771/1‑150 (MQ=255)

                                                                                      cGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgct                                                            <  2:2603173/150‑1 (MQ=255)

                                                                                          gTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGc                                                        <  2:8406421/150‑1 (MQ=255)

                                                                                              ccGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatca                                                    <  1:559779/150‑1 (MQ=255)

                                                                                                gTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtg                                                  >  2:3699597/1‑150 (MQ=255)

                                                                                                gTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtg                                                  >  1:1933719/1‑150 (MQ=255)

                                                                                                  cGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgct                                                <  1:6037554/150‑1 (MQ=255)

                                                                                                  cGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgct                                                <  2:1581826/150‑1 (MQ=255)

                                                                                                   ggCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctg                                               <  1:5016560/150‑1 (MQ=255)

                                                                                                   ggCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctg                                               <  1:2772224/150‑1 (MQ=255)

                                                                                                    gcgcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctgc                                              <  1:3190242/150‑1 (MQ=255)

                                                                                                     cgcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATgctgct                                             >  1:787444/1‑150 (MQ=255)

                                                                                                       cccTGCAAGGCTACTAAGGCGGCTGGGTCGACCAGCTCGGCCAGCGTTTACTGGA‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                           >  1:9964618/1‑150 (MQ=255)

                                                                                                        ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGtt                                          <  1:5478238/150‑1 (MQ=255)

                                                                                                              aGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTg                                    <  1:2143329/150‑1 (MQ=255)

                                                                                                                  tactacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATg                                >  2:10770574/1‑150 (MQ=255)

                                                                                                                  tactacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATg                                <  1:6992236/150‑1 (MQ=255)

                                                                                                                  tactacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGGCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATg                                >  1:1458063/1‑150 (MQ=255)

                                                                                                                   actacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGa                               >  1:3725971/1‑150 (MQ=255)

                                                                                                                    ctacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAg                              >  2:4212945/1‑150 (MQ=255)

                                                                                                                         gcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                         <  1:10029470/150‑1 (MQ=255)

                                                                                                                         gcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtgg                         >  1:267812/1‑150 (MQ=255)

                                                                                                                           ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtg                       <  1:1832224/150‑1 (MQ=255)

                                                                                                                            gcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGAGGAGCCtggtgg                      >  1:4684919/1‑150 (MQ=255)

                                                                                                                             cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGa                     <  1:7800123/150‑1 (MQ=255)

                                                                                                                              tGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                    >  1:4582480/1‑150 (MQ=255)

                                                                                                                               gggTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACg                   >  2:6956730/1‑150 (MQ=255)

                                                                                                                                     aCCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc             <  2:1903030/150‑1 (MQ=255)

                                                                                                                                       cTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgc           >  2:5233285/1‑150 (MQ=255)

                                                                                                                                        tGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgcg          >  2:11107182/1‑150 (MQ=255)

                                                                                                                                         gCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCgcgc         <  1:10932639/150‑1 (MQ=255)

                                                                                                                                         gCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCgcgc         <  2:4504149/150‑1 (MQ=255)

                                                                                                                                          cTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc        >  2:2916591/1‑150 (MQ=255)

                                                                                                                                          cTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc        <  1:3439314/150‑1 (MQ=255)

                                                                                                                                          cTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc        >  2:6246495/1‑150 (MQ=255)

                                                                                                                                           tCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCg       <  1:4969077/150‑1 (MQ=255)

                                                                                                                                              gCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt    >  1:10766163/1‑150 (MQ=255)

                                                                                                                                              gCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt    <  1:4063921/150‑1 (MQ=255)

                                                                                                                                                cAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtc  <  1:1485602/150‑1 (MQ=255)

GCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTC  >  NC_008463/3670385‑3670678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`