BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	155,993	Δ1 bp	coding (922/981 nt)	PA14_RS00700 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	155,990	0	G	.	99.8%	2651.4 / ‑2.4	536	coding (919/981 nt)	PA14_RS00700	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (156/379); total (157/379)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 549 total aligned reads displayed.

TGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAAC  >  NC_008463/155841‑156134

tggCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCGGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCg                                                                                                                                                  <  1:10694104/150‑1 (MQ=255)

       ggggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTg                                                                                                                                          >  1:2301503/1‑150 (MQ=255)

         ggATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGAc                                                                                                                                        <  1:4563170/150‑1 (MQ=255)

           aTCGGGCTGTCCATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGATTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGa                                                                                                                                      >  2:5255303/1‑150 (MQ=255)

            tCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAg                                                                                                                                     <  2:2454427/150‑1 (MQ=255)

             cGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAgg                                                                                                                                    <  1:4251193/150‑1 (MQ=255)

              gggCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTGTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAggg                                                                                                                                   <  2:10354418/150‑1 (MQ=255)

               ggCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGc                                                                                                                                  >  2:6466173/1‑150 (MQ=255)

                gCTGTCGTTTCCGGCCAACCTGCTGGAGATGCTGCACTATGCGGCGACCATCCTGGTGCTGGTCCAGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCa                                                                                                                                 <  1:5498094/150‑1 (MQ=255)

                gCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCa                                                                                                                                 <  2:5084657/150‑1 (MQ=255)

                gCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCa                                                                                                                                 >  2:10101322/1‑150 (MQ=255)

                gCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCa                                                                                                                                 <  2:2656533/150‑1 (MQ=255)

                  tGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAgg                                                                                                                               <  1:9666907/150‑1 (MQ=255)

                   gTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGt                                                                                                                              >  1:3327619/1‑150 (MQ=255)

                    tCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTACTGCTCTCCCGCAATGCCCTGCGCACCAGGATGTATGCCCCGGTTTCCCTGGGCCAGCCGAGGAAGGCC‑GGGCACTGACGAGGGCAGGtt                                                                                                                             >  2:4114415/1‑150 (MQ=255)

                      gATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttct                                                                                                                           >  2:1073163/1‑150 (MQ=255)

                       cttCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttctt                                                                                                                          <  1:7891615/149‑1 (MQ=255)

                       aTTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttctt                                                                                                                          <  1:6502619/150‑1 (MQ=255)

                        ttCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttcttc                                                                                                                         <  1:6278355/150‑1 (MQ=255)

                        ttCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGttcttc                                                                                                                         <  2:6135879/150‑1 (MQ=255)

                           cGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAt                                                                                                                      >  2:9540269/1‑150 (MQ=255)

                           cGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGAt                                                                                                                      >  1:5330232/1‑150 (MQ=255)

                                 aCCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttg                                                                                                                >  2:3212290/1‑150 (MQ=255)

                                 aCCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttg                                                                                                                >  2:3730134/1‑150 (MQ=255)

                                 aCCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCGGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttg                                                                                                                <  2:5547626/150‑1 (MQ=255)

                                   ctgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAgttgtt                                                                                                              <  2:7470747/150‑1 (MQ=255)

                                    tgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGttt                                                                                                             <  2:6934250/150‑1 (MQ=255)

                                    tgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGttt                                                                                                             <  1:8862160/150‑1 (MQ=255)

                                    tgctgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGttt                                                                                                             <  2:8701883/150‑1 (MQ=255)

                                       tgGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTaa                                                                                                          <  1:1404597/150‑1 (MQ=255)

                                         gCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACt                                                                                                        >  2:3884236/1‑150 (MQ=255)

                                           gcggctccCCGATGCGGCGACCATCCTGGTGCTGGTCCTGCTCACCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTag                                                                                                      <  2:6209548/147‑2 (MQ=255)

                                           gATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAt                                                                                                      <  2:9343017/150‑1 (MQ=255)

                                           gATGCTGCCCGATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAt                                                                                                      <  1:8522239/150‑1 (MQ=255)

                                            aTGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAtt                                                                                                     <  1:9930477/150‑1 (MQ=255)

                                            aTGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAtt                                                                                                     <  1:9483795/150‑1 (MQ=255)

                                              gctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTta                                                                                                   <  2:1287729/150‑1 (MQ=255)

                                              gctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTta                                                                                                   <  2:4471656/150‑1 (MQ=255)

                                               ctgcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtat                                                                                                  <  1:6024574/150‑1 (MQ=255)

                                                 gcCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatat                                                                                                <  1:4001869/150‑1 (MQ=255)

                                                  cccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTtatata                                                                                               <  2:1588069/150‑1 (MQ=255)

                                                   ccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTatatat                                                                                              >  1:10138290/1‑150 (MQ=255)

                                                   ccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTatatat                                                                                              >  2:7025000/1‑150 (MQ=255)

                                                   ccTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTatatat                                                                                              <  2:8165816/150‑1 (MQ=255)

                                                    cTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAtt                                                                                             <  1:10321800/150‑1 (MQ=255)

                                                       tGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttctt                                                                                          <  1:500500/150‑1 (MQ=255)

                                                        gcggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttcttc                                                                                         <  1:6064003/150‑1 (MQ=255)

                                                        gcggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATAttcttc                                                                                         <  2:10815210/150‑1 (MQ=255)

                                                         cggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCg                                                                                        >  1:8275775/1‑150 (MQ=255)

                                                         cggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCg                                                                                        <  1:4654561/150‑1 (MQ=255)

                                                         cggcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCg                                                                                        <  1:1771496/150‑1 (MQ=255)

                                                           gcgACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGAt                                                                                      >  2:1904684/1‑150 (MQ=255)

                                                             gACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAg                                                                                    <  1:4745831/150‑1 (MQ=255)

                                                             gACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAg                                                                                    <  2:6657719/150‑1 (MQ=255)

                                                                 aTCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGTCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAttt                                                                                >  2:8379443/1‑150 (MQ=255)

                                                                 aTCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAttt                                                                                <  1:7285113/150‑1 (MQ=255)

                                                                 aTCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAttt                                                                                >  2:7950733/1‑150 (MQ=255)

                                                                    cTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGa                                                                             <  2:4783834/150‑1 (MQ=255)

                                                                    cTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGa                                                                             >  1:6732917/1‑150 (MQ=255)

                                                                     tGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGAt                                                                            >  1:9187319/1‑150 (MQ=255)

                                                                      ggTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATa                                                                           <  1:11007903/150‑1 (MQ=255)

                                                                      ggTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGAATTAGATa                                                                           >  2:9745417/1‑150 (MQ=255)

                                                                         gCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAtt                                                                        <  2:2024589/150‑1 (MQ=255)

                                                                         gCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAtt                                                                        >  2:3420034/1‑150 (MQ=255)

                                                                         gCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAtt                                                                        >  1:3288575/1‑150 (MQ=255)

                                                                          cTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAttt                                                                       <  1:10461081/150‑1 (MQ=255)

                                                                          cTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAAttt                                                                       <  2:1507682/150‑1 (MQ=255)

                                                                              tCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACt                                                                   <  1:217016/150‑1 (MQ=255)

                                                                              tCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACt                                                                   <  1:5776839/150‑1 (MQ=255)

                                                                                  gCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTAGTTATATATTCTTCGATAGAGTTAGATAATTTCACTGATg                                                               >  2:10184675/1‑150 (MQ=255)

                                                                                    tctcCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGc                                                             >  1:257138/1‑150 (MQ=255)

                                                                                    tctcCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGc                                                             >  1:7994288/1‑150 (MQ=255)

                                                                                    tctcCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGc                                                             <  2:5867025/150‑1 (MQ=255)

                                                                                     ctcCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGcc                                                            <  2:2170227/150‑1 (MQ=255)

                                                                                       cccGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTa                                                          <  1:8780544/150‑1 (MQ=255)

                                                                                             aTGCCCTGCGCACCCGGCTGTATGCCCCGGGTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCaa                                                    <  1:4858466/150‑1 (MQ=255)

                                                                                               gCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATg                                                  <  2:5358725/150‑1 (MQ=255)

                                                                                               gCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATg                                                  >  1:3144926/1‑150 (MQ=255)

                                                                                                cccTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATgg                                                 <  2:7742141/150‑1 (MQ=255)

                                                                                                  cTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATgggg                                               >  1:1141586/1‑150 (MQ=255)

                                                                                                  cTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATgggg                                               >  2:2248703/1‑150 (MQ=255)

                                                                                                      gcACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAtt                                           <  2:7235619/150‑1 (MQ=255)

                                                                                                       cACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGAGGAAGGCC‑GGGCACTGACGAGGGCAGGTTATTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCAttt                                          <  2:714853/150‑1 (MQ=255)

                                                                                                          ccGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTCAATCAATGGGGCATTTTTg                                       <  1:3700411/150‑1 (MQ=255)

                                                                                                                gTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAAc                                 <  1:2032261/150‑1 (MQ=255)

                                                                                                                     ccccGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGt                            <  2:3976463/150‑1 (MQ=255)

                                                                                                                      cccGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGtt                           <  1:2983335/150‑1 (MQ=255)

                                                                                                                       ccGGTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTa                          <  1:7398159/150‑1 (MQ=255)

                                                                                                                         ggTTTCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGGTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCACTGGGGCATGTTTGCCGCACCCCGTTAAg                        >  1:6948850/1‑150 (MQ=255)

                                                                                                                           tttCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGAt                      <  1:2746970/150‑1 (MQ=255)

                                                                                                                           tttCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGAt                      <  2:1178032/150‑1 (MQ=255)

                                                                                                                           tttCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGACGAACCCCGTTAAGAt                      >  2:2862460/1‑150 (MQ=255)

                                                                                                                            ttCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGAtt                     >  1:6642069/1‑150 (MQ=255)

                                                                                                                             tCCCTGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATtc                    <  2:4314925/150‑1 (MQ=255)

                                                                                                                                 tGGGCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCATCGATTAGTTGTTTAAACTATTTATATATTCTTCGATAGATTAAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATtctcgc                >  2:3082777/1‑148 (MQ=255)

                                                                                                                                   ggCCAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATGTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAg              <  2:9438018/150‑1 (MQ=255)

                                                                                                                                     ccAGCCGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTc            <  1:10014580/150‑1 (MQ=255)

                                                                                                                                         ccGTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCCGTCGAtt        <  2:8534753/150‑1 (MQ=255)

                                                                                                                                           gTGGAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTGAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTg      >  1:1494299/1‑150 (MQ=255)

                                                                                                                                             ggAAGGCC‑GGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGaa    <  2:9190100/150‑1 (MQ=255)

TGGCCCAGGGGATCGGGCTGTCGATTCCGGCCAACCTGCTGGCGATGCTGCCCTATGCGGCGACCATCCTGGTGCTGGTCCTGCTCTCCCGCGATGCCCTGCGCACCCGGCTGTATGCCCCGGTTTCCCTGGGCCAGCCGTGGAAGGCCGGGGCACTGACGAGGGCAGGTTCTTCGATTAGTTGTTTTAACTATTTATATATTCTTCGATAGATTTAGATAATTTCACTGATGGCCTAAATCAATGGGGCATTTTTGCCGAACCCCGTTAAGATTCTCCCAGTCGATTTGAAAC  >  NC_008463/155841‑156134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`