BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_008463	5,131,729	Δ34 bp	pseudogene (288‑321/1180 nt)	PA14_RS23490 ←	acyl‑CoA dehydrogenase family protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_008463	5131729	5131762	34	593 [0]	[1] 598	PA14_RS23490	acyl‑CoA dehydrogenase family protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_008463	= 5131728	0 (0.000)	507 (1.140)	203/262	0.1	100%	pseudogene (322/1180 nt)	PA14_RS23490	acyl‑CoA dehydrogenase family protein
*	?	NC_008463	5131763 =	0 (0.000)	507 (1.140)	203/262	0.1	100%	pseudogene (287/1180 nt)	PA14_RS23490	acyl‑CoA dehydrogenase family protein

Only 100 of 598 total aligned reads displayed.

GGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGGCGCAGCGATGTCCGAATGTAGGGCGCAGCGATGTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGTTCCAG  >  NC_008463/5131614‑5131912

ccgccgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgt                                                                                                                                                       >  1:8520529‑M2/150‑150 (MQ=255)

ccgccgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgt                                                                                                                                                       <  2:3768536‑M2/1‑1 (MQ=255)

ccgccgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgt                                                                                                                                                       <  2:6532039‑M2/1‑1 (MQ=255)

  gccgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtcgtggaggatgtagggcgcagcgatgTcc                                                                                                                                                     >  2:501065‑M2/148‑150 (MQ=255)

  gccgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTcc                                                                                                                                                     <  1:3371355‑M2/3‑1 (MQ=255)

   ccgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCg                                                                                                                                                    >  1:10002388‑M2/147‑150 (MQ=255)

   ccgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCg                                                                                                                                                    >  1:9520188‑M2/147‑150 (MQ=255)

   ccgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCg                                                                                                                                                    <  2:2686783‑M2/4‑1 (MQ=255)

   ccgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCg                                                                                                                                                    >  1:4806212‑M2/147‑150 (MQ=255)

    cgtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGa                                                                                                                                                   >  2:3516552‑M2/146‑150 (MQ=255)

     gtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGaa                                                                                                                                                  >  1:6113123‑M2/145‑150 (MQ=255)

     gtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGaa                                                                                                                                                  >  1:1317315‑M2/145‑150 (MQ=255)

     gtggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGaa                                                                                                                                                  >  1:10346013‑M2/145‑150 (MQ=255)

       ggtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATg                                                                                                                                                >  1:7392790‑M2/143‑150 (MQ=255)

        gtcttcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGc                                                                                                                                               >  1:8159357‑M2/142‑150 (MQ=255)

            tcaccccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAggg                                                                                                                                           >  2:11045610‑M2/138‑150 (MQ=255)

                cccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGaa                                                                                                                                       >  2:9825956‑M2/134‑150 (MQ=255)

                cccttgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGaa                                                                                                                                       <  2:5431058‑M2/17‑1 (MQ=255)

                    tgcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCg                                                                                                                                   <  2:5664833‑M2/21‑1 (MQ=255)

                     gcaggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGa                                                                                                                                  <  2:10940898‑M2/22‑1 (MQ=255)

                       aggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATc                                                                                                                                >  2:11151231‑M2/127‑150 (MQ=255)

                        ggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATcc                                                                                                                               >  2:7977754‑M2/126‑150 (MQ=255)

                        ggtcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATcc                                                                                                                               <  2:8672773‑M2/25‑1 (MQ=255)

                          tcggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccggagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCg                                                                                                                             >  1:6614238‑M2/124‑150 (MQ=255)

                            ggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGt                                                                                                                           >  2:3310617‑M2/122‑150 (MQ=255)

                            ggaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGt                                                                                                                           >  1:10372132‑M2/122‑150 (MQ=255)

                             gaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTc                                                                                                                          <  2:8335086‑M2/30‑1 (MQ=255)

                             gaaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTc                                                                                                                          <  1:1632006‑M2/30‑1 (MQ=255)

                              aaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCa                                                                                                                         <  1:4157309‑M2/31‑1 (MQ=255)

                              aaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCa                                                                                                                         >  1:11096255‑M2/120‑150 (MQ=255)

                              aaccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCa                                                                                                                         >  1:1841177‑M2/120‑150 (MQ=255)

                                ccggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAgg                                                                                                                       <  2:7520107‑M2/33‑1 (MQ=255)

                                 cggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAggc                                                                                                                      >  2:10337930‑M2/117‑150 (MQ=255)

                                  ggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAggcc                                                                                                                     <  2:4170800‑M2/35‑1 (MQ=255)

                                  ggcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAggcc                                                                                                                     >  1:2687956‑M2/116‑150 (MQ=255)

                                   gcccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAggccg                                                                                                                    >  1:2348491‑M2/115‑150 (MQ=255)

                                     ccccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAggccggc                                                                                                                  >  2:2935210‑M2/113‑150 (MQ=255)

                                      cccggctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAggccggcc                                                                                                                 <  2:1948576‑M2/39‑1 (MQ=255)

                                          gctcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCccgac                                                                                                             <  1:6252675‑M2/43‑1 (MQ=255)

                                            tcggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCccgaccg                                                                                                           <  1:10781219‑M2/45‑1 (MQ=255)

                                             cggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCccgaccga                                                                                                          >  1:5844928‑M2/105‑150 (MQ=255)

                                             cggtcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCccgaccga                                                                                                          <  2:5074616‑M2/46‑1 (MQ=255)

                                                tcatggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGAtct                                                                                                       >  1:8810909‑M2/102‑150 (MQ=255)

                                                  atggcgatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATctct                                                                                                     >  1:8997577‑M2/100‑150 (MQ=255)

                                                       gatggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGAt                                                                                                >  2:1684311‑M2/95‑150 (MQ=255)

                                                        atggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATg                                                                                               <  2:8267891‑M2/57‑1 (MQ=255)

                                                        atggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATg                                                                                               >  2:9316184‑M2/94‑150 (MQ=255)

                                                        atggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATg                                                                                               >  1:7793551‑M2/94‑150 (MQ=255)

                                                         tggccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGa                                                                                              <  2:4957335‑M2/58‑1 (MQ=255)

                                                           gccgcgaccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGAcc                                                                                            <  1:5159637‑M2/60‑1 (MQ=255)

                                                                  ccatctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGgcgc                                                                                     <  2:3866427‑M2/67‑1 (MQ=255)

                                                                   catctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCt                                                                                    >  2:7232478‑M2/83‑150 (MQ=255)

                                                                     tctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGt                                                                                  <  1:10120968‑M2/70‑1 (MQ=255)

                                                                     tctcgccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGt                                                                                  <  1:4431090‑M2/70‑1 (MQ=255)

                                                                         gccggataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTagag                                                                              >  2:5252260‑M2/77‑150 (MQ=255)

                                                                             gataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAg                                                                          >  2:877439‑M2/73‑150 (MQ=255)

                                                                             gataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAg                                                                          <  1:7114359‑M2/78‑1 (MQ=255)

                                                                              ataccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGt                                                                         <  1:6795756‑M2/79‑1 (MQ=255)

                                                                               taccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTc                                                                        <  2:6348318‑M2/80‑1 (MQ=255)

                                                                               taccagcttcggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTc                                                                        <  1:8411103‑M2/80‑1 (MQ=255)

                                                                                        cggcaggtacttgcgcgacagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGACTGCAGGGAGACACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGAGGACCACGGCGCTGTAGAGGAAGTCCGCGCCCAt                                                               <  2:5975362‑M2/89‑1 (MQ=255)

                                                                                         ggcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATc                                                              >  2:2351767‑M2/61‑150 (MQ=255)

                                                                                          gcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATcc                                                             <  2:10704357‑M2/91‑1 (MQ=255)

                                                                                          gcaggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATcc                                                             <  1:7297602‑M2/91‑1 (MQ=255)

                                                                                           caggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATccc                                                            <  2:10657038‑M2/92‑1 (MQ=255)

                                                                                           caggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATccc                                                            <  2:7640130‑M2/92‑1 (MQ=255)

                                                                                            aggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCg                                                           >  1:10180558‑M2/58‑150 (MQ=255)

                                                                                            aggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCg                                                           >  1:2986131‑M2/58‑150 (MQ=255)

                                                                                             ggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGc                                                          >  2:9594185‑M2/57‑150 (MQ=255)

                                                                                             ggtacttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGc                                                          >  2:2088305‑M2/57‑150 (MQ=255)

                                                                                                acttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCAt                                                       <  1:1746200‑M2/97‑1 (MQ=255)

                                                                                                  ttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATAc                                                     <  2:10046660‑M2/99‑1 (MQ=255)

                                                                                                  ttgcgcttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCGGTAGAGGAAGTCCGCGCCCATCCCGCCATAc                                                     >  2:9117156‑M2/52‑150 (MQ=255)

                                                                                                       cttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTc                                                <  1:2123882‑M2/104‑1 (MQ=255)

                                                                                                        ttcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTcc                                               <  2:3063709‑M2/105‑1 (MQ=255)

                                                                                                         tcagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCg                                              <  1:8687278‑M2/106‑1 (MQ=255)

                                                                                                          cagcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCgg                                             <  1:7945906‑M2/107‑1 (MQ=255)

                                                                                                           agcgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACAACGGCGCTGTAGAGGAAGTCAGCGCCCATCCCGCCATACTCTTCCGGc                                            >  1:3761416‑M2/43‑150 (MQ=255)

                                                                                                             cgcctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAg                                          >  1:3990609‑M2/41‑150 (MQ=255)

                                                                                                               cctcgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGt                                        <  2:10436590‑M2/112‑1 (MQ=255)

                                                                                                                ctcgctgccgtagtggaggaggtagggcgcagcgatgTCCGAATGCAGGGAGAACCCGATCCAGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTg                                       >  2:6169111‑M2/38‑150 (MQ=255)

                                                                                                                  cgctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCg                                     <  1:4909784‑M2/115‑1 (MQ=255)

                                                                                                                    ctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGaa                                   <  1:6345351‑M2/117‑1 (MQ=255)

                                                                                                                    ctgccgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGaa                                   >  2:8470918‑M2/34‑150 (MQ=255)

                                                                                                                        cgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACaga                               >  1:8088585‑M2/30‑150 (MQ=255)

                                                                                                                        cgtagtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACaga                               <  1:356675‑M2/121‑1 (MQ=255)

                                                                                                                           agtggaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCa                            >  1:5009169‑M2/27‑150 (MQ=255)

                                                                                                                               gaggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGACCAGAGCATTcc                        >  2:6939923‑M2/23‑150 (MQ=255)

                                                                                                                                 ggatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCGACGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCgg                      <  2:7740224‑M2/130‑1 (MQ=255)

                                                                                                                                  gatgtagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGc                     <  1:2839714‑M2/131‑1 (MQ=255)

                                                                                                                                      tagggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCg                 >  2:1337880‑M2/16‑150 (MQ=255)

                                                                                                                                       agggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGc                >  1:7531601‑M2/15‑150 (MQ=255)

                                                                                                                                       agggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGc                >  1:5516256‑M2/15‑150 (MQ=255)

                                                                                                                                       agggcgcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGc                <  1:9830908‑M2/136‑1 (MQ=255)

                                                                                                                                            gcagcgatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGcc           >  1:11137539‑M2/10‑150 (MQ=255)

                                                                                                                                             cAGCGATGTCCGAATGCAGGGAGAACCCGATCCCGGTCCGGCCGGCCCGACCGATCTCTTAGATGACCACGGCGCGGTAGAGGACGTCCGCGCCCATCCCGCCATACTCGAGCGGCAGGGGCGCCCAGAGCATTCCGGGCTCGCCGGccg          >  2:6773106/1‑149 (MQ=255)

                                                                                                                                                 gatgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGtt      >  1:933014‑M2/5‑150 (MQ=255)

                                                                                                                                                   tgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGTTcc    >  2:6380982‑M2/3‑150 (MQ=255)

                                                                                                                                                   tgTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGTTcc    >  1:10538000‑M2/3‑150 (MQ=255)

                                                                                                                                                    gTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGTTCCa   >  2:4669627‑M2/2‑150 (MQ=255)

GGCCCCCGGCTCGGTCATGGCGATGGCCGCGACCATCTCGCCGGATACCAGCTTCGGCAGGTACTTGCGCTTCAGCGCCTCGCTGCCGTAGTGGAGGATGTAGGGCGCAGCGATGGCGCAGCGATGTCCGAATGTAGGGCGCAGCGATGTCCGAATGCAGGGAGAAACCGATCCCGGTCAGGCCGGCCCGACCGATCTCTTCGATGACCACGGCGCTGTAGAGGAAGTCCGCGCCCATCCCGCCATACTCTTCCGGCAGGTGCGAACAGAGCATTCCGGCCTCGCCGGCCTTGTTCCAG  >  NC_008463/5131614‑5131912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`