Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463867,3780AC23.2% 746.0 / 24.7 387L390R (CTG→CGG) PA14_RS04080MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (232/39);  new base C (25/57);  total (257/130)
Fisher's exact test for biased strand distribution p-value = 7.03e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 393 total aligned reads displayed.

CTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGCT  >  NC_008463/867229‑867526
                                                                                                                                                     |                                                                                                                                                    
ctctATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCa                                                                                                                                                      >  1:1356695/1‑150 (MQ=255)
  ctATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAgg                                                                                                                                                    >  1:10134259/1‑150 (MQ=255)
  ctATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAgg                                                                                                                                                    >  1:2267999/1‑150 (MQ=255)
    aTCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGgcg                                                                                                                                                  >  2:4856249/1‑150 (MQ=255)
     tCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTTCTGAACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGgcgc                                                                                                                                                 >  1:7879024/1‑150 (MQ=255)
     tCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGgcgc                                                                                                                                                 <  1:7029931/150‑1 (MQ=255)
      ccGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGgcgcg                                                                                                                                                >  2:7896382/1‑150 (MQ=255)
        gTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGcgcgcg                                                                                                                                              <  1:6153693/150‑1 (MQ=255)
         tCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGcga                                                                                                                                             <  2:9757237/150‑1 (MQ=255)
         tCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCCCGcga                                                                                                                                             >  2:5750968/1‑150 (MQ=255)
           gCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGcgacg                                                                                                                                           >  1:461777/1‑150 (MQ=255)
              ccGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGacgacg                                                                                                                                        >  1:5410311/1‑150 (MQ=255)
               cGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACgg                                                                                                                                       >  1:11056835/1‑150 (MQ=255)
                 gCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGGCGCGCCAGGCGCGCGACGACgggg                                                                                                                                     >  2:4182005/1‑150 (MQ=255)
                    tCCGTCGACGATCGTCTGGTCCTACCTGGATTTCGCCCATCAGCACCTGGACGTGCTCCCCGAGGCCTGGCGGCTGGACGGCCCTGCGCCTGGCGGGGGGTAGCACGCTGGGGTCCGCGGCGGCGCGCCCGGCCCGCGACGACGGGGagc                                                                                                                                  <  2:2390098/150‑1 (MQ=255)
                    tCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGagc                                                                                                                                  >  1:7577292/1‑150 (MQ=255)
                     ccGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGagca                                                                                                                                 >  1:7390604/1‑150 (MQ=255)
                      cGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGagcag                                                                                                                                >  2:1557730/1‑150 (MQ=255)
                       gTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGagcagc                                                                                                                               >  2:1145542/1‑150 (MQ=255)
                        tCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGAAGGGGAGCAgcg                                                                                                                              >  1:406169/1‑150 (MQ=255)
                              aTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGAc                                                                                                                        >  2:4316906/1‑150 (MQ=255)
                              aTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGAc                                                                                                                        >  2:11046469/1‑150 (MQ=255)
                               tCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCTCCAGAcc                                                                                                                       >  1:5018104/1‑150 (MQ=255)
                                 gTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGa                                                                                                                     >  2:8077373/1‑150 (MQ=255)
                                   ctggTGCTACGTGTATTTCTGCCCGCAGCACCTGGACCTGCTGCACGAGGCGTGTCGGCTGGACGGCCCTGCGCCTGGGGGGGGGTCGGACGGTGGCGTCAGCGTCGTCGCGCCGGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGt                                                                                                                   <  2:399151/148‑1 (MQ=255)
                                   ccggTGCTCCCTGGATTACGCCCCGCAGCACCGGCACCTGCTCCACTCGGCCTGAGGGCGGGCCGGCCCTGCGCCGGGCGCGGGTTCGCACGCTGGCGTCCGCGCCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGt                                                                                                                   <  2:699963/148‑1 (MQ=255)
                                   cAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGt                                                                                                                   >  1:10504290/1‑150 (MQ=255)
                                      gTGCTTCGTGGTTTTCGGCCAGCCCCACGGGGCCCGGCTCCACGCTGCCTGTGGGGGGGACGGCCCTGCGCGTGGCGCGGGGTCGGCGGCGGGCGTCAGCGTCGCCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACg                                                                                                                <  2:3189785/150‑1 (MQ=255)
                                       tGCTACCTGTATTTCTGCCAGCACCACCTGGCCCTGCTGCACTTTGCCTGTCGGCGGGAGGGCCCTGCGCGTGGCGCGGGGTTGGACGGGGGCGTCAGGGTGGTCGCGCCGGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACgg                                                                                                               <  2:543740/150‑1 (MQ=255)
                                       tGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGTCCGAGTACgg                                                                                                               >  2:6627948/1‑150 (MQ=255)
                                       tGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACgg                                                                                                               >  2:4438676/1‑150 (MQ=255)
                                       tGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACgg                                                                                                               >  2:338882/1‑150 (MQ=255)
                                        gCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCAGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCCGCGCCAGACCGAGTACGGc                                                                                                              >  2:6672716/1‑150 (MQ=255)
                                        catACCGGGATTCCGGCCCGCCGCCCGGGGACCGGCGGCCGGAGGCGTGTCGGCGGGACGGCCCTGCGCCGGCCGGGGGTTAGGCCGCGGGCGTCCGCGTCGCCCCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGc                                                                                                              <  2:6088086/148‑1 (MQ=255)
                                         cTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCg                                                                                                             >  1:642304/1‑150 (MQ=255)
                                           aCCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGAAGGGGAGCAGCGCCAGGCCGTGTACGGCgtg                                                                                                           >  1:1199143/1‑148 (MQ=255)
                                            ccTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAgg                                                                                                          >  2:7939620/1‑150 (MQ=255)
                                             cTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGTCCGAGTACGGCGCggg                                                                                                         >  2:6478048/1‑150 (MQ=255)
                                                gATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCCGACCGAGTACGGCGAGGGCCg                                                                                                      >  2:4761502/1‑150 (MQ=255)
                                                 ttttcgcccaccaGCCCCGGGCCCTGCTGCTCGAGGCGTGTGGGGGGGACGGCCGTGCGCCGGGCGGGGGGTTGGAGGCGGGCGTCTGCGTGGTCGGGCCGGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGc                                                                                                     <  2:1356922/143‑1 (MQ=255)
                                                   ttCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTg                                                                                                   >  2:10864771/1‑150 (MQ=255)
                                                     cTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGcg                                                                                                 >  1:9749035/1‑150 (MQ=255)
                                                      tGCCCGCAGCACCTGCCCCGGCTGCCCGTTGCCTGTCGGCTGGACGGCCCTGCGCGTGGCGGGGGTTGGGACGCTGGCTTCAGCGTGGTCGCGCCCGCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGcga                                                                                                <  1:7243990/150‑1 (MQ=255)
                                                         ctgctgcaCCTGGCCGTGCTCCCCGAGGCCTGTGGGCTGTACGGCCCAGCGCCTGGGGCGGGGTAGGACGCTGGCTGCAGCGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGcgacga                                                                                             <  2:11131226/145‑1 (MQ=255)
                                                         cagcagCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGcgacga                                                                                             >  2:10751953/1‑150 (MQ=255)
                                                              gcaCCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAATCCg                                                                                        >  1:631333/1‑150 (MQ=255)
                                                                  cTGGCCCTGCTGCCCGAGGCCTGGCGGCTGGACGTCCGTGCGCCGGGCGGGGGGACGGCCGCGGGCTTCCGGGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTc                                                                                    <  2:515980/150‑1 (MQ=255)
                                                                     gcccgggtgcCCGATGCCTGTCGGCTGGCGGGCCCGGCGCGTGGCGGGGGGTCGGCCGCTGGCGTCCCCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGtt                                                                                 <  2:8509944/143‑1 (MQ=255)
                                                                       ccTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGGACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTcc                                                                               >  1:875274/1‑150 (MQ=255)
                                                                             gcACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCCGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCg                                                                         >  2:6849470/1‑150 (MQ=255)
                                                                              cACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCga                                                                        >  1:8160206/1‑150 (MQ=255)
                                                                               aCGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCgag                                                                       >  2:2455695/1‑150 (MQ=255)
                                                                                 gATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCCGCGCCCGACCGGGTACGGCGAGGGGCGCTGCGCCGAAGCCGCTTCGTTCCCGGCCGagag                                                                     >  1:3801344/1‑150 (MQ=255)
                                                                                 gATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCCGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGGTCCAGGCCGagag                                                                     >  2:9101110/1‑150 (MQ=255)
                                                                                  aTGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGTCGCCCGACGACCGGGAGCAGCGCCAGACCGAGTACTGCGAGGGCCTCAGCGACGAAGCCGATTCGTTCCAGGCCGagaga                                                                    >  2:3656481/1‑149 (MQ=255)
                                                                                  aTGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGt                                                                    >  2:9477694/1‑150 (MQ=255)
                                                                                   tggcggTCGCCTGGCCGCCCCTCCGCCTGGGGCGGGGTCGTCGGCTGGCGTACGCTTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTc                                                                   <  1:1669071/145‑1 (MQ=255)
                                                                                    gCCTGTCGGTGGGACGGCCCGGCGCGGGGCGGGGGGTCGGCCGCGGGCGTCAGCGTCGCCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTcg                                                                  <  2:7539506/150‑1 (MQ=255)
                                                                                        gTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGAAGTCGGGGAGCAGCGCCAGTCCGAGTACGGCGAGGGCCGCTGCGTCGAAGCCGATTCGGTCCAGGCCGAGAGTCGCGAt                                                              >  1:671192/1‑150 (MQ=255)
                                                                                         tCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGGAGCGCCAGACCGAGTAAGGCGAGGGCCGCGGCGACGAAGCCGCTTCGTTCCAGGCCGCGAGTCGCGATg                                                             >  1:2016379/1‑150 (MQ=255)
                                                                                         ggggCTGGCCGCCCCGGCGCCGGGCGGGGGGGAGGCCGGGGGCGTCAGGGTCGGCGCCCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATg                                                             <  1:4141829/148‑1 (MQ=255)
                                                                                            gctgtccGGCCGTGCGCCTGGCGCGGGTTTGGCCGCGGGCGTCTGCGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtc                                                          <  2:1441577/144‑1 (MQ=255)
                                                                                              tGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGTCCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGTGTCGCGATGGtctc                                                        >  2:10612953/1‑150 (MQ=255)
                                                                                                   ggCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCCGAACGCGTACGGCGCGGGGCGCGGCGACGAAGCCGATTGGTTGCAGGCCGAGAGTAGCGATGGTCTCTCCGc                                                   >  1:3137485/1‑150 (MQ=255)
                                                                                                    gCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGcc                                                  >  2:4948748/1‑150 (MQ=255)
                                                                                                    gCCCGGCGCCGGGGGGGGGGTTGGCGGCGGGCGCCCGGGTGGTCGCCCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGcc                                                  <  1:594853/150‑1 (MQ=255)
                                                                                                     cccTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGGTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCa                                                 >  2:8055289/1‑150 (MQ=255)
                                                                                                         gcgcCGGGGGGGGGGTAGGACGGTGGCGACTGCGCCGGCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTa                                             <  2:2823977/150‑1 (MQ=255)
                                                                                                           gcgtGGCGGGGGGTTGGACGCGGGGGTCTGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgc                                           <  1:1482665/147‑1 (MQ=255)
                                                                                                            cggggCGGGGGGTTGGCCGCTGGCGTCAGGGTCTTCGCCCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgcg                                          <  1:9006074/147‑1 (MQ=255)
                                                                                                             cTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgcgc                                         >  2:8842248/1‑150 (MQ=255)
                                                                                                             cTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgcgc                                         >  1:6356672/1‑150 (MQ=255)
                                                                                                              tGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTGGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCCTGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGGTCCAGGCCGAGTGTCGCGATGGTCTCTCCGCCAAGTAgcgcg                                        >  1:7772594/1‑150 (MQ=255)
                                                                                                              tGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTAGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAtcgcg                                        >  1:7117458/1‑150 (MQ=255)
                                                                                                                  gcgGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGTCGACGGGGAGCAGCGCCCGACCGCGTACGGCGTGGGCCGCTGCGCCGTAGCCGGTTCGTTCCAGGCCGAGTGTCGCGCTGGGCGCGCCGCCAAGTAGCGCGCCgc                                    >  2:10264685/1‑149 (MQ=255)
                                                                                                                   cgGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCCGCGCCAGACCGCGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGGTCCAGGCCCAGAGTCGCGATGGGCTCTCCGCCAAGTAGCGCGCCGAg                                   >  1:6088436/1‑150 (MQ=255)
                                                                                                                         cggACGCGGGCGACCGGGTCGTCCCCCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGAt                             <  1:516632/149‑1 (MQ=255)
                                                                                                                          ggACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCCGCGACGGGGAGCCGCGCCAGACCGATTACGGCGAGGGCCGCCGCGACGAAGCCCATTCGTTACAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGGAGCGCGCCGAGGCCgcac                            >  1:10817056/1‑147 (MQ=255)
                                                                                                                            ccgcgGGCTTCTGCGTCGTGGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATccc                          <  2:7101855/149‑1 (MQ=255)
                                                                                                                             cGCTGGCGTCCGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCg                         <  2:7244268/150‑1 (MQ=255)
                                                                                                                                 ggCGTCAGCGTCGTCGCCCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCAt                     <  2:8636689/150‑1 (MQ=255)
                                                                                                                                   cgactgCGTCGCGGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTg                   <  1:5745811/145‑1 (MQ=255)
                                                                                                                                      ccgCGTCGCCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcg                <  1:8461845/148‑1 (MQ=255)
                                                                                                                                      cAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcg                >  2:824451/1‑150 (MQ=255)
                                                                                                                                        gCGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggc              <  2:4816072/150‑1 (MQ=255)
                                                                                                                                        gCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCCATTAGTTCCAGGCCGAGAGTCGCGATGGTCTCTACGCCAAGTAGCGCGCCGAGGCCGATCCCGGCAATggcggc              >  2:7478071/1‑150 (MQ=255)
                                                                                                                                         cgtcgtCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcg             <  1:4410587/150‑1 (MQ=255)
                                                                                                                                         cgtcggCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcg             <  2:10990192/150‑1 (MQ=255)
                                                                                                                                          gtcgtcGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcgg            <  2:2594653/150‑1 (MQ=255)
                                                                                                                                          ggcgtcgCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcgg            <  1:4873379/148‑1 (MQ=255)
                                                                                                                                           tcgtcgCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcggc           <  2:5845238/150‑1 (MQ=255)
                                                                                                                                           tcgtcgCGCCCGGGGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcggc           <  2:677953/150‑1 (MQ=255)
                                                                                                                                           tcgtcgCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcggc           >  2:3563987/1‑150 (MQ=255)
                                                                                                                                           tcgtcgCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcggc           >  1:2447499/1‑150 (MQ=255)
                                                                                                                                           tcgtcgCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcggc           >  1:10031855/1‑150 (MQ=255)
                                                                                                                                           gcgccgcgCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcggcggc           <  1:4251254/146‑1 (MQ=255)
                                                                                                                                             gtcgCGCCGGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGa         <  2:9655329/150‑1 (MQ=255)
                                                                                                                                                gcgcCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGt      <  2:7705936/150‑1 (MQ=255)
                                                                                                                                                  gcCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTg    >  1:969364/1‑150 (MQ=255)
                                                                                                                                                   ccAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGc   <  2:2053145/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGCT  >  NC_008463/867229‑867526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑