Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,910,2560AC23.8% 743.1 / 24.4 308intergenic (+55/‑90)aceE/aceFpyruvate dehydrogenase (acetyl‑transferring), homodimeric type/dihydrolipoyllysine‑residue acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (172/62);  new base C (5/68);  total (177/131)
Fisher's exact test for biased strand distribution p-value = 1.61e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 314 total aligned reads displayed.

TGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAGCTG  >  NC_008463/5910107‑5910405
                                                                                                                                                     |                                                                                                                                                     
 ggCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAc                                                                                                                                                      <  2:6222380/150‑1 (MQ=255)
 ggCAGATCGCGGCGATATCGAGCCGTAAGTGGTGGCCGAGGCCCTCGCCAAGTACGGTATCGATCCGGAGAATCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCATCGGCAGCTAGATGTTACTAGCACGCCCGCGCCTCAAAc                                                                                                                                                      >  2:3314424/1‑150 (MQ=255)
  gCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACg                                                                                                                                                     <  1:6306620/150‑1 (MQ=255)
   ccGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACgg                                                                                                                                                    <  1:7524687/150‑1 (MQ=255)
   ccGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACgg                                                                                                                                                    >  2:7663283/1‑150 (MQ=255)
    cGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACggg                                                                                                                                                   <  1:8370782/150‑1 (MQ=255)
    cGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACggg                                                                                                                                                   <  1:9922766/150‑1 (MQ=255)
      aTCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTCTCGATCCGGAGAAGCGCAACCCGCTGGATTGCTCATTCGCTCCATACTCCCCTCGGCAGCGAGGGGAGTCGAGCACGCCGGCGCCGCCCACGggcg                                                                                                                                                 >  2:1024169/1‑150 (MQ=255)
      aTCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGggcg                                                                                                                                                 >  2:3372/1‑150 (MQ=255)
      aTCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGggcg                                                                                                                                                 >  2:6819729/1‑150 (MQ=255)
       tCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGggcgg                                                                                                                                                <  2:9346598/150‑1 (MQ=255)
         gcggcgATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGgcggcg                                                                                                                                              <  1:1859102/150‑1 (MQ=255)
         gcggcgATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGgcggcg                                                                                                                                              >  2:673082/1‑150 (MQ=255)
          cggcgATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGGACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATCCTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGgcgc                                                                                                                                             <  1:5595531/150‑1 (MQ=255)
            gcgATATCGCGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCg                                                                                                                                           <  1:6437169/150‑1 (MQ=255)
            gcgATATCGAGCCGAACGGGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGATAAGCGCAACCCGGTGGTTTGCTAATTCGCTCCATACTCCCCTCGGAAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCg                                                                                                                                           <  1:4106758/150‑1 (MQ=255)
              gttatCGAGCCGAACGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGc                                                                                                                                         >  2:11006948/3‑150 (MQ=255)
               atatCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCt                                                                                                                                        >  1:1284235/1‑150 (MQ=255)
               atatCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCt                                                                                                                                        >  2:6377626/1‑150 (MQ=255)
                tatCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTc                                                                                                                                       >  2:1742322/1‑150 (MQ=255)
                  tCGATCCTAATGGGGTGGCCGTGGCCATCGCAAAGTAGGGTATCTATCCGTAGACGCGCACCCCGCTGTTTTGATATTTCGCTCCATACTCCCCTCGGCTGGGTGGGTTTTCGTGCACGCCGGCGCCGCCACGGGGCGGCGCCGGCTcac                                                                                                                                     <  2:3564576/150‑1 (MQ=255)
                   cGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGGTcaca                                                                                                                                    >  1:10470410/1‑150 (MQ=255)
                   cGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTcaca                                                                                                                                    >  2:5403433/1‑150 (MQ=255)
                   cGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCCACCGGCGGCGCCGGCGcaca                                                                                                                                    >  1:2865035/1‑150 (MQ=255)
                   cGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCCCCCCCCCCGGGCGGCCCCCGCGcacc                                                                                                                                    >  1:8452192/1‑149 (MQ=255)
                          aaaGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAAcc                                                                                                                             >  2:3828886/1‑150 (MQ=255)
                          aaaGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACCGGCGGCGCCCGCGCACCACGAAcc                                                                                                                             >  1:7708918/1‑150 (MQ=255)
                           aaGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGCGCGGCCCCCGCTCACAACGAAACa                                                                                                                            >  1:908423/1‑150 (MQ=255)
                              tggtggCCTAGGCAATCGCCACGTCCGGTATCGATCCGTAGTTGCGCAACCCGCGGTTTTGCTAATTCGCTCCATCCTCCCCTCGGCAGCGAGGGGTTTCGACCACGCGGGCGCCCCAAACGGGCGGCGCCGGCTCACAACGAACCAAgg                                                                                                                         <  2:6123742/150‑1 (MQ=255)
                                gtggCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAAACAAGGAc                                                                                                                       >  1:6978326/1‑150 (MQ=255)
                                 tggCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGAcc                                                                                                                      >  2:820421/1‑150 (MQ=255)
                                       aGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGt                                                                                                                >  2:4980829/1‑150 (MQ=255)
                                       aGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAAAGAACCAAGGACCaaaatt                                                                                                                >  2:2053310/1‑148 (MQ=255)
                                         gCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCt                                                                                                              >  2:2681465/1‑150 (MQ=255)
                                          ccATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCAACGGGCGGCGGCGGCTCACAACGAACCAAGGACCAACAGTcgt                                                                                                             >  2:6984285/1‑148 (MQ=255)
                                                  aaGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTgc                                                                                                     >  2:10067403/1‑150 (MQ=255)
                                                    ctagtgTATCGACCCGGAGACGCCCACCCCGCGGTTTTGTTAATTCGCCCAAAACCCCCCCCGGCAGGGAGGGTTTTCGAGCACGCCGGCGCCGCCACCGGGCGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTgcgc                                                                                                   <  2:279849/145‑1 (MQ=255)
                                                     tACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAAGGGCGGCGCCGGCTCACAACGAAACAATGGCCAAAATTTTTTGTTTTgcgcg                                                                                                  >  1:4851994/1‑150 (MQ=255)
                                                        ggTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCAACGGGGGGCGCCGGCTCACAACGAAACCAGGACCAAAAGTGGTTTTTTTGCGcgccg                                                                                               >  1:682845/1‑150 (MQ=255)
                                                           acctaTCGGGATAAGCGCAACCCGCTGTTTTCAAAATCCGCCCAATACTCCCCTCGGCGGCGTGGGTTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCTCAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGcac                                                                                            <  2:852508/146‑1 (MQ=255)
                                                           aTCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGGCGCCAACGGGCGGGGCCGGCTCACAAAGAAACAAGGACCAAAAGTCTTTGTTTTGCGCGCCGcac                                                                                            >  1:5352687/1‑150 (MQ=255)
                                                           aTCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACCGGCGGCGCCGGCGCACAACGAACCAAGGGACAAAAGTAGTTGTTTTGCCCGCCGcac                                                                                            >  1:5971024/1‑150 (MQ=255)
                                                               aTCCGGTGACTCCCAACCCGCTTGTTGGCTAATTCGCTCCATACCCCCCGCGGCAGCGTGGGTTTTCGAGACCGCCGGCGCCGCCACGGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCg                                                                                        <  2:6344228/150‑1 (MQ=255)
                                                               aTCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCCCAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCg                                                                                        >  1:5484895/1‑150 (MQ=255)
                                                                tCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCgg                                                                                       >  2:4119721/1‑150 (MQ=255)
                                                                  cgcagaCGCGCAACCGGGGGTATTGCTACTTCGCTCCATCCTCCCCGCGGATGCGTGGGGTTGCGTGCCCGCCGGCGCCGCAACCGGGCGGCGCCGGCTCACACCGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCggtg                                                                                     <  2:8698589/147‑1 (MQ=255)
                                                                   ggAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCAATGTTTTGCGCGCCGCACAGCggtgg                                                                                    >  2:10675401/1‑150 (MQ=255)
                                                                   ggAGAACCCAAACCGGCTGTTTTGATAATACGCTCAATCCCCCCCACGGCCGCGTGGGGTTTCGAGCACGCCGGCGCCGCCACGGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCggtgg                                                                                    <  1:10120571/150‑1 (MQ=255)
                                                                    gagaCGCCCACCCCGCTGTTTTGATAATCCGCACCAAACCCCCCGCGCCAGCGTGGGTTTTCGCCCCCGCGGCCGCCCCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCggtggt                                                                                   <  1:8192921/150‑1 (MQ=255)
                                                                           cccaCCCGCGGGTTTGCTACTGCGCACCATCCTCCCCTCGGCTGCGAGGGTTTTGGACCACGCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGt                                                                            <  2:9632147/147‑1 (MQ=255)
                                                                             accccGCGGGATTCCTAATTCGCCCCAACCGCCCCGCGGCCGCGCGGGGTGACGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTaa                                                                          <  2:6167671/148‑1 (MQ=255)
                                                                                 cggggttttGCTTATTCGCTCCAAACCCCCCGCGGCTGCGGGGGTTTTCGCGCCCGCCGGCGCCGCCACCGGGGGGGGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAgg                                                                      <  2:1953493/143‑1 (MQ=255)
                                                                                 cGCTGGTTGGCTAATTCGCTCCATCCTCCCCTCGGCAGCGTGGGGTTTCGCGCCCGCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAgg                                                                      <  2:6885217/150‑1 (MQ=255)
                                                                                   cTGTATTGATAATTCGCTCCAAACCCCCCTCGGCCGCGCGGGGTTTCGCCCACGCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGgag                                                                    <  1:8271359/150‑1 (MQ=255)
                                                                                   cTGGATTGCTATTCCGCACCAGACACCCCTCGGCCGCGTGGGGTTTCGCGCACGCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGgag                                                                    <  2:5492198/150‑1 (MQ=255)
                                                                                    tGGATTCCTAAGCCGCCCCACCCCCCCCGCGCCCGGGTGGGGTTTCTCCCCCGCCGGCCCCGCCACGGGGGGGCGCCGGCTCACACCGCACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGgaga                                                                   <  2:10396194/150‑1 (MQ=255)
                                                                                     ggATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGGAAAAGGagag                                                                  >  2:2531564/1‑150 (MQ=255)
                                                                                     ggATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGTCCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGGGGCCCGGACAAGGagag                                                                  >  2:8113712/1‑150 (MQ=255)
                                                                                       aTTGATACTTCGCTCAACACGCCCCTCGGCCGCGTGGGGTTTCGAGCCCGCGGGCGCCGCCACGGGGGGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGa                                                                <  1:3490227/150‑1 (MQ=255)
                                                                                          gatAATTCGCTCCATACTCCCCTCGGCAGCGTGGGGTTTCGAGCACGCCGGCGCCCCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAAtg                                                             <  2:1402998/148‑1 (MQ=255)
                                                                                          gCTAATTCGCTCCATACTCCCCTCGGCTGCGTGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGGGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAAtg                                                             <  2:4564845/150‑1 (MQ=255)
                                                                                          gCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCACACCACGAACCAAGGGCCAAAAGTATTTGTTTTGCGCGCCGCCCAGCGGGGGTGGCCCGGATAAGGAGAGGGAtg                                                             >  1:4486418/1‑150 (MQ=255)
                                                                                            tAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCTCACAACGAAACAAGGACCAAAATTATTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAAtgtg                                                           >  2:9831937/1‑150 (MQ=255)
                                                                                            tAATTCGCTCCAACCTCCCCTCGGCCGCGCGGGTTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAAtgtg                                                           <  2:5877355/150‑1 (MQ=255)
                                                                                             aaTTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCCCCCAAGGGCGGCGCCGGCGCAAAACCAACCAAGGAACCAAATTCTTTGTTTTGGGCCCCGCACAACGGTGGTGGGCCGGAAAAGGAGAgggaagggt                                                          >  1:9380196/1‑143 (MQ=255)
                                                                                              tttCCCTCCATACGCCCCTGGGCTGCGTGGGGTTTGGCCCACGCCGGCGCCGCCATCGGGGGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAg                                                         <  2:7568746/149‑1 (MQ=255)
                                                                                                tCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACCGCGGTGGTGGCACGTAAAAGGGGAGGAATTTGAGCg                                                       >  2:4723738/1‑150 (MQ=255)
                                                                                                 cGCTCCATACGCCCCTCGGCAGCGAGGGGTTTCGCGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGa                                                      <  2:7226998/150‑1 (MQ=255)
                                                                                                  gCTCCATACTCCCCTCGGCCGGTAGGGGTTTCGAGCACGCGGGCGCCGCCACCGGGCGGCGCCGGCTCACACCGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGaa                                                     <  2:2391661/150‑1 (MQ=255)
                                                                                                   cTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCCAAAGTACTTGTTTTGGGCGCCCCACAGCGGGGGGGGCACGGAAAAGGAGAGGAATGTGAGGGAAc                                                    >  1:4361451/1‑150 (MQ=255)
                                                                                                     ccATAATCCCCTCGCCAGCGAGGGGTTTCGACCACGCCGCCGCCGCAAAGGGGGGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTc                                                  <  2:4528764/150‑1 (MQ=255)
                                                                                                              ccgcGGCTGCGTGGGGTTTCGAGCCCGCGGGCGCCCCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTa                                         <  2:1476127/147‑1 (MQ=255)
                                                                                                              ccgcGGCCGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACCGGCGGCGCCGGGAGCCGGCGAACCAAGGACCCAAAGTCGTTGTTTTGCGCGGCGCACAGCGGTGGTGGCAGGGAAACGGAGTGGTATGTGATCGGACTCATTCGGGtg                                         >  2:1132485/4‑149 (MQ=255)
                                                                                                              ccTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAAAGGGCGGCGCCGGCGCACAAAGCAACCAGGACCAAAATTATTTGTTTTGCGCGGCCCACAGCGGGGGTGGGACGGAAAAGGAGATGACTGTGAGCGTACACATTAGCGTa                                         >  1:1529271/1‑150 (MQ=255)
                                                                                                                   gCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCTCACAACGAACCAAGGACCCAAAGTCTTTGTTTTGCGCGCCGCACCGCGGGGGTGGCACGTCAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGa                                    >  2:9926582/1‑150 (MQ=255)
                                                                                                                     aGCGAGGGGTTTCGAGCACGCCGGCGCCGCCCACGGGCGGCGCCGGCTCACAACGAACCAAGGACCCAAAGGCTTTGTTTTGGGCGCCGCACAGCGGTGGTGGCACGGAAAAGGTGAGGGATGGGGGCGCACGCATTAGCGTTCCCggcc                                  >  1:10038586/1‑147 (MQ=255)
                                                                                                                     aGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTGGTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGGAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACa                                  >  2:4504707/1‑150 (MQ=255)
                                                                                                                      gCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTGTTTGTTTTGCGCCCCGCACAGCGGTGGGGGCCAGGAAAAGGCGCGGAAAGTGAGCGAACGCAGTCGCGTACCCGACAt                                 >  2:7987947/1‑150 (MQ=255)
                                                                                                                        gtgggtTTTCGAGCACGCCGGCGCCGCCAAGGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCg                               <  2:11152194/148‑1 (MQ=255)
                                                                                                                               ttCGCGCCCGCGGGCGCCCCCACGGGGGGGCCCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACgg                        <  1:10826429/150‑1 (MQ=255)
                                                                                                                                  gAGCACGCCGGCGCCCCCAACGGGCGGCCCCGGCTCACAACGAACCCAGGACCCAAAGTAGTTGTTTTGCGCGCCGCACAGCGGGGGGGGCCCGGCAAAGGAGCGGAAAGTGAGCGAACACAATCGCGTAACCGACAACCGCCACGGTGa                     >  2:1543473/1‑150 (MQ=255)
                                                                                                                                   cgCACGCGGGCCCCGCAACGGGGGGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAg                    <  1:8965361/149‑1 (MQ=255)
                                                                                                                                   cgCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAg                    <  2:4849415/149‑1 (MQ=255)
                                                                                                                                     cACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGAACAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGGACGTAAAAGGAGAGGAATGTTAGCGAACACATTAGCGTACCCGACAGCGGCAACGGTGAggg                  >  1:6412739/1‑150 (MQ=255)
                                                                                                                                      aCGCCGGCCCCCCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGt                 <  1:8680087/150‑1 (MQ=255)
                                                                                                                                       cGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTg                >  2:10800325/1‑150 (MQ=255)
                                                                                                                                        gCCGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGa               <  1:4156032/150‑1 (MQ=255)
                                                                                                                                         ccGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGGAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTAACCCACATCGGCAACGGTGAGGGTGaa              >  1:8618206/1‑150 (MQ=255)
                                                                                                                                          cGGCGCCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCAGTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAg             <  2:9065021/150‑1 (MQ=255)
                                                                                                                                          cGGCGCCCCCACGGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAg             <  2:2084360/150‑1 (MQ=255)
                                                                                                                                           ggCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCACCGGTGAGGGTGAAGt            >  2:8810316/1‑150 (MQ=255)
                                                                                                                                           ggCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGt            >  2:4915265/1‑150 (MQ=255)
                                                                                                                                            gcgcCGCCACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTc           <  1:3352092/150‑1 (MQ=255)
                                                                                                                                              gccgccACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCAt         <  2:1585502/150‑1 (MQ=255)
                                                                                                                                                cgccAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCg       >  2:9179464/1‑150 (MQ=255)
                                                                                                                                                 gccAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGa      >  1:8052528/1‑150 (MQ=255)
                                                                                                                                                 gccAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGa      >  2:6660559/1‑150 (MQ=255)
                                                                                                                                                  ccACCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAACGACTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCTCGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAg     <  2:9166852/150‑1 (MQ=255)
                                                                                                                                                   cAACGGGCGGCGCCGGCTCACAACAAAACAAGGACCAAAAGAATTTTTTTTTCGCGCCGCCCAGCCGTGGTGGCACGTAAAAGGAGAGGAATTTTATCGAACACAATAGCGTACCCCACAAACGCAACCGTGAGGGTTAACTAATccaac    >  2:1876690/1‑146 (MQ=255)
                                                                                                                                                    aaCGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAgct   <  2:8907519/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCCGATCGCGGCGATATCGAGCCGAAAGTGGTGGCCGAGGCCATCGCCAAGTACGGTATCGATCCGGAGAAGCGCAACCCGCTGGATTGCTAATTCGCTCCATACTCCCCTCGGCAGCGAGGGGTTTCGAGCACGCCGGCGCCGCCAACGGGCGGCGCCGGCTCACAACGAACCAAGGACCAAAAGTCTTTGTTTTGCGCGCCGCACAGCGGTGGTGGCACGTAAAAGGAGAGGAATGTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAGCTG  >  NC_008463/5910107‑5910405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑