Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,357 A→G L314L (TTG→CTG)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,3570AG99.6% 1804.9 / ‑7.1 467L314L (TTG→CTG) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (222/243);  minor base T (1/1);  total (223/244)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 467 total aligned reads displayed.

AGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCC  >  NC_008463/2955210‑2955503
                                                                                                                                                   |                                                                                                                                                  
aGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGc                                                                                                                                                  <  2:2924386/150‑1 (MQ=255)
 gATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCg                                                                                                                                                 <  1:5841591/150‑1 (MQ=255)
 gATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCg                                                                                                                                                 >  1:24046/1‑150 (MQ=255)
  agcagcagGCCGCTGAGCACCAACATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCgg                                                                                                                                                <  1:3186342/148‑1 (MQ=255)
  aTCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCgg                                                                                                                                                <  2:2741009/150‑1 (MQ=255)
      gcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCCGGGGGGTACGGTCGCTGAGCAGGCGggtgg                                                                                                                                            >  2:6332663/1‑150 (MQ=255)
      gcagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtgg                                                                                                                                            <  2:6852108/150‑1 (MQ=255)
       cagGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGggtggt                                                                                                                                           <  1:7008613/150‑1 (MQ=255)
         ggCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGttt                                                                                                                                         <  1:9874000/150‑1 (MQ=255)
         ggCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCGTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGAGTCGGTGACGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGttt                                                                                                                                         >  2:4571661/1‑150 (MQ=255)
          gCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTc                                                                                                                                        <  1:1868168/150‑1 (MQ=255)
           ccGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCg                                                                                                                                       >  1:1193552/1‑150 (MQ=255)
           ccGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCg                                                                                                                                       >  1:10802055/1‑150 (MQ=255)
            cGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGa                                                                                                                                      <  1:6536238/150‑1 (MQ=255)
             gCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGAt                                                                                                                                     <  2:3056655/150‑1 (MQ=255)
                gAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCtt                                                                                                                                  <  2:4061462/150‑1 (MQ=255)
                  gCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGAAGGCGGGTGGTTTCGATCTTGc                                                                                                                                >  1:9364974/1‑150 (MQ=255)
                    aCCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCg                                                                                                                              >  1:6791986/1‑150 (MQ=255)
                     ccATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCgg                                                                                                                             <  2:6514325/150‑1 (MQ=255)
                     ccATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCgg                                                                                                                             >  1:3825458/1‑150 (MQ=255)
                       atcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGta                                                                                                                           <  2:6987931/150‑1 (MQ=255)
                       atcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGta                                                                                                                           <  2:1407737/150‑1 (MQ=255)
                        tcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGtat                                                                                                                          >  1:7303292/1‑150 (MQ=255)
                        tcatGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGtat                                                                                                                          <  2:4823295/150‑1 (MQ=255)
                         catGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGtata                                                                                                                         <  2:5909166/150‑1 (MQ=255)
                           tGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAg                                                                                                                       >  2:6050259/1‑150 (MQ=255)
                            gCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAgg                                                                                                                      >  2:9680011/1‑150 (MQ=255)
                                  cagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAg                                                                                                                <  1:3651661/150‑1 (MQ=255)
                                  cagcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAg                                                                                                                <  1:8853018/150‑1 (MQ=255)
                                   agcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgc                                                                                                               <  1:5905849/150‑1 (MQ=255)
                                   agcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgc                                                                                                               <  2:1589919/150‑1 (MQ=255)
                                   agcagGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgc                                                                                                               >  1:2054059/1‑150 (MQ=255)
                                    gcagGCCGAAGACGCACCAGATCAGCTTGACCCAGATCCCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGACGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAgcg                                                                                                              <  2:3478578/150‑1 (MQ=255)
                                       ggCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGACCTGCAGGGGGGTCCGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCgg                                                                                                           >  2:9384203/1‑150 (MQ=255)
                                                cGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGa                                                                                                  <  2:5711792/150‑1 (MQ=255)
                                                   aCCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACa                                                                                               <  2:10303192/150‑1 (MQ=255)
                                                   aCCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACa                                                                                               <  1:10560947/150‑1 (MQ=255)
                                                     cAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATg                                                                                             >  1:6926977/1‑150 (MQ=255)
                                                      aGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGa                                                                                            <  1:6354928/150‑1 (MQ=255)
                                                       gATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGTCTGGACCATGAg                                                                                           >  2:1738476/1‑150 (MQ=255)
                                                        aTCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGc                                                                                          <  2:3178467/150‑1 (MQ=255)
                                                         tCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTACAGCGCGGCCGACTGGAACATGAGCg                                                                                         <  2:3587156/150‑1 (MQ=255)
                                                          cAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACCGGAACATGAGCgg                                                                                        <  1:1409539/150‑1 (MQ=255)
                                                            gCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGAt                                                                                      >  2:2463793/1‑150 (MQ=255)
                                                             cTTGACCCAGCTACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGGCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATACGGGTTCAGCGCGGCCGACTGGAACAGGCGCGGATa                                                                                     <  2:817605/150‑1 (MQ=255)
                                                              ttGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATAc                                                                                    >  2:7542688/1‑150 (MQ=255)
                                                              ttGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATAc                                                                                    >  1:2636501/1‑150 (MQ=255)
                                                               tGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATAcc                                                                                   <  2:9134029/150‑1 (MQ=255)
                                                                gACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCa                                                                                  >  1:2907008/1‑150 (MQ=255)
                                                                  cccAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGc                                                                                >  1:9807707/1‑150 (MQ=255)
                                                                   ccAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGACCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGcc                                                                               >  1:2798633/1‑150 (MQ=255)
                                                                            gccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGc                                                                      >  2:193732/1‑150 (MQ=255)
                                                                            gccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGc                                                                      <  1:7720854/150‑1 (MQ=255)
                                                                             ccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTAGAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGcc                                                                     >  2:7261265/1‑150 (MQ=255)
                                                                                 aaTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGa                                                                 >  2:8879449/1‑150 (MQ=255)
                                                                                 aaTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGa                                                                 <  1:4711999/150‑1 (MQ=255)
                                                                                 aaTCGCCGGTGGGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGa                                                                 >  1:5237200/1‑150 (MQ=255)
                                                                                  aTCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGaa                                                                >  1:745951/1‑150 (MQ=255)
                                                                                   tCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAAc                                                               <  1:2184368/150‑1 (MQ=255)
                                                                                       cGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCg                                                           >  1:8950196/1‑150 (MQ=255)
                                                                                        ggTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCgg                                                          >  1:5751003/1‑150 (MQ=255)
                                                                                        ggTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCgg                                                          >  2:8923571/1‑150 (MQ=255)
                                                                                         gtgtGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCggg                                                         <  1:5579091/150‑1 (MQ=255)
                                                                                         gtgtGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCggg                                                         <  2:4352777/150‑1 (MQ=255)
                                                                                          tgtgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGc                                                        >  2:4920374/1‑150 (MQ=255)
                                                                                            tgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTg                                                      <  1:345419/150‑1 (MQ=255)
                                                                                               aGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAg                                                   >  1:8248549/1‑150 (MQ=255)
                                                                                                 gggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGc                                                 <  2:8738357/150‑1 (MQ=255)
                                                                                                 gggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGc                                                 >  1:9192273/1‑150 (MQ=255)
                                                                                                      gCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGc                                            >  2:8514192/1‑150 (MQ=255)
                                                                                                       cATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGcc                                           >  1:1565262/1‑150 (MQ=255)
                                                                                                          ggATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCggg                                        <  1:6077615/150‑1 (MQ=255)
                                                                                                          ggATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGGATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCggg                                        >  2:5109145/1‑150 (MQ=255)
                                                                                                           gATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCGGTAGGCGTTGCCGGGc                                       >  2:544506/1‑150 (MQ=255)
                                                                                                            aTTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCa                                      >  2:2066140/1‑150 (MQ=255)
                                                                                                             ttCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCag                                     >  1:1663171/1‑149 (MQ=255)
                                                                                                             ttCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCag                                     >  2:2823703/1‑149 (MQ=255)
                                                                                                                 gTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAac                                 >  1:10263541/1‑150 (MQ=255)
                                                                                                                  tGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAaca                                <  1:4001758/150‑1 (MQ=255)
                                                                                                                   gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAacac                               <  1:10055124/150‑1 (MQ=255)
                                                                                                                   gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAacac                               <  2:8552384/150‑1 (MQ=255)
                                                                                                                   gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAacac                               >  1:8579719/1‑150 (MQ=255)
                                                                                                                     cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcg                             >  2:11142307/1‑150 (MQ=255)
                                                                                                                     cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcg                             <  2:7405704/150‑1 (MQ=255)
                                                                                                                     cGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTGTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcg                             <  2:8325240/150‑1 (MQ=255)
                                                                                                                           gCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTgg                       >  2:7028742/1‑150 (MQ=255)
                                                                                                                             aGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGcc                     >  1:7863094/1‑150 (MQ=255)
                                                                                                                              ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCCGGAACACCGCGCTGGCCt                    >  1:10687685/1‑150 (MQ=255)
                                                                                                                              ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCt                    >  1:11089075/1‑150 (MQ=255)
                                                                                                                              ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCt                    <  2:3731235/150‑1 (MQ=255)
                                                                                                                               gggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTc                   <  2:10055427/150‑1 (MQ=255)
                                                                                                                                      cGGTCACTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTg            >  2:8028175/1‑150 (MQ=255)
                                                                                                                                        gTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTggg          >  1:4535239/1‑150 (MQ=255)
                                                                                                                                         tCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgggg         <  2:10889359/150‑1 (MQ=255)
                                                                                                                                            cTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATa      <  2:10793140/150‑1 (MQ=255)
                                                                                                                                             tGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc     >  1:8689484/1‑150 (MQ=255)
                                                                                                                                             tGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATAc     >  1:7627207/1‑150 (MQ=255)
                                                                                                                                              gAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACg    <  2:1061076/150‑1 (MQ=255)
                                                                                                                                              gAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACg    >  1:3096484/1‑150 (MQ=255)
                                                                                                                                                gCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGcc  >  2:10123546/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGATCAGCAGGCCGCTGAGCACCATCATGCTCAGCAGCAGGCCGAAGACGAACCAGATCAGCTTGACCCAGATACCGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCC  >  NC_008463/2955210‑2955503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑